北京市售桶装饮用水中双酚A的污染水平
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摘要
目的建立定量分析桶装饮用水中双酚A的方法,研究北京市桶装饮用水中双酚A的污染水平。方法采用固相萃取技术进行样品前处理,运用液相色谱?质谱联用法进行样品检测。结果在质量浓度为122.9~1190.7 ng/L范围内,在最优的质谱检测条件下,一级质谱的线性回归方程为y=69.4x+2186.1,相关系数为0.993,检出限为7.0 ng/L,定量限为23.5 ng/L。方法的回收率为78.8%~108.1%,相对标准偏差(n=6)≤13.5%。在随机检测的北京市6个水站的53种桶装饮用水中有36种检出双酚A,但浓度均低于《生活饮用水卫生标准》(GB 5749—2006)中规定的双酚A限值(0.01 mg/L)。结论该方法选择性好、灵敏度高、重现性好,能够满足定量检测桶装饮用水中双酚A的要求。北京市售桶装水中较为普遍地存在双酚A,长期饮用有健康风险。
The work aims to establish a quantitative method for detecting bisphenol A in barrelled drinking water and to investigate the pollution level of bisphenol A in barrelled drinking water products sold in Beijing market. After sample pretreatment by a solid phase extraction method, bisphenol A was detected by liquid chromatography-tandem mass spectrometry. Under the optimal mass spectrum conditions for bisphenol A detection, the linear regression equation of level 1 mass spectrometry was y=69.4x+2186.1 with the correlation coefficient of 0.993 within the concentration range of 122.9~1190.7 ng/L. The limit of detection was 7.0 ng/L, and the limit of quantitation was 23.5 ng/L. The recoveries were between 78.8% and 108.1%, and the relative standard deviations(n=6) were less than 13.5%. Among 53 barrelled drinking water products from 6 water stations in Beijing, bisphenol A was detected in 36 products, but the concentrations were all lower than 0.01 mg/L that was the concentration limit required in Standards for Drinking Water Quality(GB 5749—2006). Owing to the advantages of excellent selectivity, high sensitivity and good reproducibility, this method can meet the detection demands of bisphenol A in barrelled drinking water. Bisphenol A widely exists in barrelled drinking water products sold in Beijing market, and long-term drinking thus leads to health risks.
The work aims to establish a quantitative method for detecting bisphenol A in barrelled drinking water and to investigate the pollution level of bisphenol A in barrelled drinking water products sold in Beijing market. After sample pretreatment by a solid phase extraction method, bisphenol A was detected by liquid chromatography-tandem mass spectrometry. Under the optimal mass spectrum conditions for bisphenol A detection, the linear regression equation of level 1 mass spectrometry was y=69.4x+2186.1 with the correlation coefficient of 0.993 within the concentration range of 122.9~1190.7 ng/L. The limit of detection was 7.0 ng/L, and the limit of quantitation was 23.5 ng/L. The recoveries were between 78.8% and 108.1%, and the relative standard deviations(n=6) were less than 13.5%. Among 53 barrelled drinking water products from 6 water stations in Beijing, bisphenol A was detected in 36 products, but the concentrations were all lower than 0.01 mg/L that was the concentration limit required in Standards for Drinking Water Quality(GB 5749—2006). Owing to the advantages of excellent selectivity, high sensitivity and good reproducibility, this method can meet the detection demands of bisphenol A in barrelled drinking water. Bisphenol A widely exists in barrelled drinking water products sold in Beijing market, and long-term drinking thus leads to health risks.
引文
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[17]陈虎,念东,甘一萍,等.北京市再生水与地表水中的内分泌干扰物分析[J].环境科学与技术,2014,37(S2):352—356.CHEN Hu,NIAN Dong,GAN Yi-ping,et al.Analysis of Endocrine Disrupting Chemicals in Beijing City of Reclaimed Water and Surface Water[J].Environmental Science&Technology,2014,37(S2):352—356.
[18]周自严,黄仁德,钟嶷,等.广州市饮用水中双酚A及邻苯二甲酸酯的调查[J].环境与健康杂志,2015,32(1):43—45.ZHOU Zi-yan,HUANG Ren-de,ZHONG Yi,et al.Investigation of Bisphenol A and Phthalates in Drinking Water in Guangzhou[J].Journal of Environment and Health,2015,32(1):43—45.
[19]董金狮.饮用水桶现状、问题及发展趋势[J].中国包装,2011(4):66—70.DONG Jin-shi.The Situation,Problems and Development Trend of Drinking Water Barrel[J].China Packaging,2011(4):66—70.
[20]王君,许超,慕春玲,等.重复使用下聚碳酸酯饮水桶中双酚A的迁移规律[J].包装工程,2016,37(9):44—48.WANG Jun,XU Chao,MU Chun-ling,et al.Migration of Bisphenol A from Polycarbonate(PC)Drinking Bucket under the Reused Condition[J].Packaging Engineering,2016,37(9):44—48.
[21]CANDACE R,NORA K,HUGUES P'H.Is Container Type the Biggest Predictor of Trace Element and BPA Leaching from Drinking Water Bottles?[J].Food Chemistry,2016,202:88—93.
[22]FREDERICK S S,WADE V W.Evidence that Bisphenol A(BPA)can be Accurately Measured without Contamination in Human Serum and Urine,and that BPA Causes Numerous Hazards from Multiple Routes of Exposure[J].Environment,Epigenetics and Reproduction,2014,398(1/2):101—113.
[2]MATTHEW L,AMOLD S,OLAF P,et al.Exposure Assessment of Adult Intake of Bisphenol A(BPA)with Emphasis on Canned Food Dietary Exposures[J].Environment International,2015,77:55—62.
[3]董文丽,王伟华,巩雪,等.PC制品中双酚A定量分析方法的研究[J].包装工程,2014,35(5):5—8.DONG Wen-li,WANG Wei-hua,GONG Xue,et al.Quantitative Analysis Method of Bisphenol A in PC Products[J].Packaging Engineering,2014,35(5):5—8.
[4]孙汉文,李挥,高文慧,等.食品包装材料中双酚A迁移量的测定[J].食品科学,2012,33(2):235—239.SUN Han-wen,LI Hui,GAO Wen-hui,et al.Determination of Bisphenol A Migration in Food Packaging Materials[J].Food Science,2012,33(2):235—239.
[5]孙希岚,朱争礼,单营营,等.高效液相色谱-荧光检测法检测金属食品罐用涂料中的双酚A含量[J].包装工程,2013,34(3):27—30.SUN Xi-lan,ZHU Zheng-li,SHAN Ying-ying,et al.Determination of Bisphenol A in Coatings of Metal Food Cans by HPLC Fluorescence Detection Method[J].Packaging Engineering,2013,34(3):27—30.
[6]FREDERICK S S,SUSAN C N,BENJAMIN L C,et al.The Estrogenic Endocrine Disrupting Chemical Bisphenol A(BPA)and Obesity[J].Environment,Epigenetics and Reproduction,2012,354(1/2):74—84.
[7]JUDY S L,DANIEL Q N.Temporal Trends in Bisphenol A Exposure in the United States from 2003—2012and Factors Associated with BPA Exposure:Spot Samples and Urine Dilution Complicate Data Interpretation[J].Environmental Research,2015,142:84—95.
[8]张彦丽,任佳丽,李忠海,等.食品包装材料中双酚A的研究进展[J].食品与机械,2011,27(1):155—157.ZHANG Yan-li,REN Jia-li,LI Zhong-hai,et al.Progress on Bisphenol A in Food Packing Materirals[J].Food and Machinery,2011,27(1):155—157.
[9]PFEIFER D,CHUNG Y M,HU M C-T.Effects of Low-dose Bisphenol A on DNA Damage and Proliferation of Breast Cells:the Role of c-Myc[J].Environmental Health Perspectives,2015,123(12):1271—1279.
[10]KUNDAKOVIC M,GUDSNUK K,FRANKS B,et al.Sex-specific Epigenetic Disruption and Behavioral Changes Following Low-dose in Utero Bisphenol A Exposure[J].Proceedings of the National Academy of Sciences of the United States of America,2013,110(24):9956—9961.
[11]JINHUA L,KATHERINE Z F,SAI V,et al.Studying Developmental Neurotoxic Effects of Bisphenol A(BPA)Using Embryonic Stem Cells[J].Journal of Environmental Sciences,2015,36:173—177.
[12]VICENTE M,ROCíO P L,NICOLáS O,et al.Bisphenol A:Human Exposure and Neuro Behavior[J].Neuro Toxicology,2015,49:174—184.
[13]郭永梅.双酚A的危害及相关限制法规[J].现代食品科技,2012,28(5):549—551.GUO Yong-mei.Analysis of Health Hazards,Legal Restrictions and Regulations for BPA[J].Modern Food Science and Technology,2012,28(5):549—551.
[14]徐述坚.桶装饮用水卫生质量状况[J].实用预防医学,2015,22(1):124—126.XU Shu-jian.Analysis on the Hygienic Quality of Bottled Drinking Water[J].Practical Preventive Medicine,2015,22(1):124—126.
[15]葛耀溶.瓶装水、桶装水造假为何屡禁不止[J].中国防伪报道,2015(10):112—118.GE Yao-rong.Why Bottled Water and Barrelled Water Repeated Fraud[J].China Anti-Counterfeiting Report,2015(10):112—118.
[16]王元元.北京“水站”黑幕[N].民主与法制时报,2014-03-24(10).WANG Yuan-yuan.The Shady of Water Stations in Beijing[N].The Democracy and Law Times,2014-03-24(10).
[17]陈虎,念东,甘一萍,等.北京市再生水与地表水中的内分泌干扰物分析[J].环境科学与技术,2014,37(S2):352—356.CHEN Hu,NIAN Dong,GAN Yi-ping,et al.Analysis of Endocrine Disrupting Chemicals in Beijing City of Reclaimed Water and Surface Water[J].Environmental Science&Technology,2014,37(S2):352—356.
[18]周自严,黄仁德,钟嶷,等.广州市饮用水中双酚A及邻苯二甲酸酯的调查[J].环境与健康杂志,2015,32(1):43—45.ZHOU Zi-yan,HUANG Ren-de,ZHONG Yi,et al.Investigation of Bisphenol A and Phthalates in Drinking Water in Guangzhou[J].Journal of Environment and Health,2015,32(1):43—45.
[19]董金狮.饮用水桶现状、问题及发展趋势[J].中国包装,2011(4):66—70.DONG Jin-shi.The Situation,Problems and Development Trend of Drinking Water Barrel[J].China Packaging,2011(4):66—70.
[20]王君,许超,慕春玲,等.重复使用下聚碳酸酯饮水桶中双酚A的迁移规律[J].包装工程,2016,37(9):44—48.WANG Jun,XU Chao,MU Chun-ling,et al.Migration of Bisphenol A from Polycarbonate(PC)Drinking Bucket under the Reused Condition[J].Packaging Engineering,2016,37(9):44—48.
[21]CANDACE R,NORA K,HUGUES P'H.Is Container Type the Biggest Predictor of Trace Element and BPA Leaching from Drinking Water Bottles?[J].Food Chemistry,2016,202:88—93.
[22]FREDERICK S S,WADE V W.Evidence that Bisphenol A(BPA)can be Accurately Measured without Contamination in Human Serum and Urine,and that BPA Causes Numerous Hazards from Multiple Routes of Exposure[J].Environment,Epigenetics and Reproduction,2014,398(1/2):101—113.
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