地震患者耐甲氧西林金黄色葡萄球菌基因分型及同源性比较研究
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摘要
目的了解从地震受伤患者分离到的耐甲氧西林金黄色葡萄球菌(MRSA)基因特征,并与同期非灾区患者分离的MRSA进行比较。方法用葡萄球菌盒式染色体(SCCmec)多重PCR分型技术进行基因分型,并检测其杀白细胞毒素基因(PVL),采用Diversilab进行同源性比较,并进行药敏分析。结果地震受伤患者与非灾区患者分离到的MRSA均表现为SCCmecⅢ型,PVL阴性;同源性分析显示,分离到的MRSA与社区获得性MRSA代表株中MLST分型ST8型同源性相对较好。结论医院获得性MRSA与社区获得性MRSA可能存在交叉传播,在灾后急救现场及当地医院要加强无菌观念,高度警惕医源性交叉感染的传播。
OBJECTIVE To investigate the genetic feature of meticillin-resistant Staphylococcus aures isolated from earthquake victims and compare them with those of common patients.METHODS The MRSA isolates underwent SCCmec(Staphylococcal Cassette Chromosome mec) multi-PCR and PVL gene detection.Diversilab method was used for homology comparison and antibiotic resistance was also analyzed.RESULTS All the MRSA isolates were confirmed to be type Ⅲ SCCmec with PVL gene negative.Homology analysis showed relatively consistent with community-acquired MRSA confirmed to be ST8 by MLST typing.CONCLUSIONS There may be cross-spread of MRSA between hospital and community.We should pay more attation even in emergent time.
OBJECTIVE To investigate the genetic feature of meticillin-resistant Staphylococcus aures isolated from earthquake victims and compare them with those of common patients.METHODS The MRSA isolates underwent SCCmec(Staphylococcal Cassette Chromosome mec) multi-PCR and PVL gene detection.Diversilab method was used for homology comparison and antibiotic resistance was also analyzed.RESULTS All the MRSA isolates were confirmed to be type Ⅲ SCCmec with PVL gene negative.Homology analysis showed relatively consistent with community-acquired MRSA confirmed to be ST8 by MLST typing.CONCLUSIONS There may be cross-spread of MRSA between hospital and community.We should pay more attation even in emergent time.
引文
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[6]Shore A,Rossney AS,Keane CT,et al.Seven novel vari-ants of the staphylococcal cassette chromosome mec in methi-cillin-resistantStaphylococcus aureusisolates from Ireland[J].Antimicrob Agents Chemother,2005,49:2070-2083.
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[9]Hanssen AM,Kjeldsen G,Johanna U,et al.Local variantsof staphylococcal cassette chromosome mec types in sporadicmethicillin-resistantStaphylococcus aureusand methicillin-re-sistant coagulase negativeStaphylococcus:evidence of Hori-zontal gene transfer[J].Antimicrob agents Chemother,2004,48:285-296.
[10]Ko KS,Lee JW,Suh JY,et al.Distribution of major geno-types among methicillin-resistantStaphylococcus aureusclones in Asian countries[J].J Clin Microbiol,2005,43:421-426.
[11]Francis JS,Garroll K.Community-acquired MRSA:a notableadversary[J].South Med J,2005,98(110):1061.
[12]Enright MC,Robinson DA.The evolutionary history of me-thicillin-resistantStaphylococcus aureus(MRSA)[J].ProcNatl Acad Sci USA,2002,99:7687-7692.
[13]巴特尔,郭淑英,张亚荣.耐甲氧西林凝固酶阴性葡萄球菌产酶耐药基因分子流行病学研究[J].中华医院感染学杂志,2005,15(6):605-607.
[14]韩黎,朱士俊,郭燕红,等.中国医务人员执行手卫生的现状调查[J].中华医院感染学杂志,2006,16(2):140-142.
[2]Jonas D,Grundmann H,Hartung D,et al.Evaluation of themecA femB duplex polymerase chain reaction for detection ofmethicillin-resistantStaphylococcus aureus[J].Eur J ClinMicrobiol Infect Dis,1999,18:643-647.
[3]Zhang K,McClure JA,Elsayed S,et al.Novel multiplex PCRassay for characterization and concomitant subtyping of staphylo-coccal cassette chromosome mec typesⅠtoⅤin methicillin-resist-antStaphylococcus aureus[J].J Clin Microbiol,2005,42:5026-5033.
[4]Lina G,Piemont Y,Godail-Gamot F,et al.Involvement ofpanton-valentine leukocidin producingStaphylococcus aureusin primary skin infections and pneumonia[J].Clin Infect Dis,1999,29:1128-1132.
[5]李囡,范红,陈慧莉,等.三株社区获得性耐甲氧西林金黄色葡萄球菌的基因分型研究[J].中华医学杂志,2008,88(20):1380-1383.
[6]Shore A,Rossney AS,Keane CT,et al.Seven novel vari-ants of the staphylococcal cassette chromosome mec in methi-cillin-resistantStaphylococcus aureusisolates from Ireland[J].Antimicrob Agents Chemother,2005,49:2070-2083.
[7]Daum RS,Ito T,Hiramatsu K,et al.A novel of methicillin-resistance cassette chromosome mec in community-acquiredmethicillin-resistantStaphylococcus aureusisolates of diversegenetic bakgrounds[J].J Infect Dis,2002,186:1344-1347.
[8]Ito T,Ma XX,Takeuchi F,et al.Novel typeⅤstaphylo-coccal cassette chromosome mec driven by a novel cassettechromosome recombinase,ccrC[J].Antimicrob Agents Che-mother,2004,48:2637-2651.
[9]Hanssen AM,Kjeldsen G,Johanna U,et al.Local variantsof staphylococcal cassette chromosome mec types in sporadicmethicillin-resistantStaphylococcus aureusand methicillin-re-sistant coagulase negativeStaphylococcus:evidence of Hori-zontal gene transfer[J].Antimicrob agents Chemother,2004,48:285-296.
[10]Ko KS,Lee JW,Suh JY,et al.Distribution of major geno-types among methicillin-resistantStaphylococcus aureusclones in Asian countries[J].J Clin Microbiol,2005,43:421-426.
[11]Francis JS,Garroll K.Community-acquired MRSA:a notableadversary[J].South Med J,2005,98(110):1061.
[12]Enright MC,Robinson DA.The evolutionary history of me-thicillin-resistantStaphylococcus aureus(MRSA)[J].ProcNatl Acad Sci USA,2002,99:7687-7692.
[13]巴特尔,郭淑英,张亚荣.耐甲氧西林凝固酶阴性葡萄球菌产酶耐药基因分子流行病学研究[J].中华医院感染学杂志,2005,15(6):605-607.
[14]韩黎,朱士俊,郭燕红,等.中国医务人员执行手卫生的现状调查[J].中华医院感染学杂志,2006,16(2):140-142.
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