Screening, Identification, and Potential Interaction of Active Compounds from Eucommia ulmodies Leaves Binding with Bovine Serum Albumin
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- 作者：Yuping Zhang ; Mijun Peng ; Liangliang Liu ; Shuyun Shi ; Sheng Peng
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：March 28, 2012
- 年：2012
- 卷：60
- 期：12
- 页码：3119-3125
- 全文大小：319K
- 年卷期：v.60,no.12(March 28, 2012)
- ISSN：1520-5118
文摘
The aqueous extract of Eucommia ulmoides leaves has been commonly known as Du-zhong tea as a functional health food for the treatment of hypertension, hypercholesterolemia, and fatty liver. This study developed a centrifugal ultrafiltration鈥揾igh-performance liquid chromatography (HPLC) method for screening and identification of bioactive compounds in E. ulmoides leaves binding with bovine serum albumin (BSA). Six active compounds were screened, isolated, and elucidated by their ultraviolet (UV), electrospray ionization鈥搈ass spectrometry (ESI鈥揗S), and nuclear magnetic resonance (NMR) data as geniposidic acid (1), caffeic acid (2), chlorogenic acid (3), quercetin-3-O-sambubioside (4), rutin (5), and isoquercitrin (6). The interaction between active compounds and BSA was investigated in the absence and presence of other compounds by quenching the intrinsic BSA fluorescence. The results indicated that the structures significantly affected the binding process. The values of binding constants for compounds 2鈥?b>6 were in the range of 105鈥?06 mol L鈥?, while geniposidic acid (1) hardly quenching the BSA intrinsic fluorescence. However, the quenching process of geniposidic acid was easily affected in the presence of other active compounds. The formation of the geniposidic acid鈥損henylpropanoid (flavonoid) complex could increase the binding affinity of geniposidic acid with BSA; however, the increased steric hindrance of the complex may make phenylpropanoid or flavonoid dissociate from BSA and then decrease their affinities.