Detection of Thrombin with an Aptamer-Based Macromolecule Biosensor Using Bacterial Ghost System
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- 作者：Jiasheng Wang ; Ke Ding ; Yujie Chen ; Lifeng Zhang ; Zukai Liu ; Angli Xue ; Wenjia Gu ; Xiaoyue Yang ; Xihan Li ; Jin Huang ; Congcong Xing ; Yunlong Cao ; Ming Chen
- 刊名：ACS Synthetic Biology
- 出版年：2014
- 出版时间：December 19, 2014
- 年：2014
- 卷：3
- 期：12
- 页码：963-965
- 全文大小：173K
- ISSN：2161-5063
文摘
A rapid on-site detection of exogenous proteins without the need for equipped laboratories or skilled personnel would benefit many areas. We built a rapid protein detection platform based on aptamer-induced inner-membrane scaffolds dimerization by virtue of bacterial ghost system. When the detection platform was coincubated with two kinds of aptamers targeting two different sites of thrombin, green fluorescence or 尾-lactamase activity were yielded with two different designs. The latter was detected by commercially available testing strips.