Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens

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• 作者：Atray Dixit¹ ; ² ; ⁹ ; Oren Parnas¹ ; ⁹ ; ¹⁰ ; Biyu Li¹ ; Jenny Chen¹ ; ² ; Charles P. Fulco¹ ; ⁴ ; Livnat Jerby-Arnon¹ ; Nemanja D. Marjanovic¹ ; ³ ; Danielle Dionne¹ ; Tyler Burks¹ ; Raktima Raychowdhury¹ ; Britt Adamson⁵ ; Thomas M. Norman⁵ ; Eric S. Lander¹ ; ⁴ ; ⁶ ; Jonathan S. Weissman⁵ ; ⁷ ; Nir Friedman¹ ; ⁸ ; Aviv Regev¹ ; ⁶ ; ⁷ ; ¹¹ ; ^{aregev@broadinstitute.org}
• 关键词：single-cell RNA-seq ; pooled screen ; CRISPR ; epistasis ; genetic interactions
• 刊名：Cell
• 出版年：2016
• 出版时间：15 December 2016
• 年：2016
• 卷：167
• 期：7
• 页码：1853-1866.e17
• 全文大小：5792 K
• 卷排序：167

文摘

Pooled CRISPR screen with scRNA-seq readout Integrated model of perturbations, single cell phenotypes, and epistatic interactions Effect of TFs on genes, programs, and states in LPS response in immune cells Downsampling assessment of feasibility of genome-wide or combinatorial screens