Safety of the intravenous administration of neurotensin-polyplex nanoparticles in BALB/c mice

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• 作者：Maria E. Hernandez ; PhD^a ; Jesus D. Rembao ; MD ; PhD^b ; Daniel Hernandez-Baltazar ; PhD^f ; Rosa A. Castillo-Rodriguez ; MSc^f ; Victor M. Tellez-Lopez ; MSc^f ; Yazmin M. Flores-Martinez ; MSc^f ; Carlos E. Orozco-Barrios ; PhD^f ; Hector A. Rubio ; MD ; PhD^c ; Aurora Sá ; nchez-Garcí ; a ; BSc^b ; Jose Ayala-Davila ; BSc^f ; Martha L. Arango-Rodriguez ; PhD^d ; Lenin Pav&oacute ; n ; PhD^a ; Teresa Mejia-Castillo ; PhD^f ; Patricia Forgez ; PhD^e ; Daniel Martinez-Fong ; MD ; PhD^f ; ^g ; ^{dmartine@fisio.cinvestav.mx" class="auth_mail} ; ^{martinez.fong@gmail.com" class="auth_mail}
• 关键词：Nanomaterials ; Pro-inflammatory cytokines ; Biosafety ; Cytotoxicity
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：May 2014
• 年：2014
• 卷：10
• 期：4
• 页码：745-754
• 全文大小：1847 K

文摘

Neurotensin (NTS)-polyplex is a gene nanocarrier that has potential nanomedicine-based applications for the treatment of Parkinson's disease and cancers of cells expressing NTS receptor type 1. We assessed the acute inflammatory response to NTS-polyplex carrying a reporter gene in BALB/c mice. The intravenous injection of NTS-polyplex caused the specific expression of the reporter gene in gastrointestinal cells. Six hours after an intravenous injection of propidium iodide labeled-NTS-polyplex, fluorescent spots were located in the cells of the organs with a mononuclear phagocyte system, suggesting NTS-polyplex clearance. In contrast to lipopolysaccharide and carbon tetrachloride, NTS-polyplex did not increase the serum levels of tumor necrosis factor alpha, interleukin (IL)-1尾, IL-6, bilirubin, aspartate transaminase, and alanine transaminase. NTS-polyplex increased the levels of serum amyloid A and alkaline phosphatase, but these levels normalized after 24 h. Compared to carrageenan, the local injection of NTS-polyplex did not produce inflammation. Our results support the safety of NTS-polyplex.

From the Clinical Editor

This study focuses on the safety of neurotensin (NTS)-polyplex, a gene nanocarrier that has potential in the treatment of Parkinson's disease and cancers of cells expressing NTS receptor type 1. NTS polyplex demonstrates a better safety profile compared with carrageenan, lipopolysaccharide, and carbon tetrachloride in a murine model.