An optimized multiplex flow cytometry protocol for the analysis of intracellular signaling in peripheral blood mononuclear cells
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- 作者：Richard Daviesa ; Richard.Davies@uib.no" class="auth_mail" title="E-mail the corresponding author ; Petra Vogelsanga ; 1 ; Roland Jonssona ; b ; Silke Appela
- 关键词：Flow cytometry ; Signaling ; Phosphorylation ; Multiparameter ; Barcoding ; PBMC
- 刊名：Journal of Immunological Methods
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：436
- 期：Complete
- 页码：58-63
- 全文大小：990 K
文摘
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Optimized flow cytometry protocol for the study of intracellular signaling in PBMC
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The protocol was shown to be precise, robust and reliable.
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Intracellular phosphorylation effect of 3 blood collection tubes are evaluated.