Comparison of real-time PCR and ELISA-based methods for the detection of beef and pork in processed meat products
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- 作者：Adam T. Perestam ; Kayleigh K. Fujisaki ; Omar Nava ; Rosalee S. Hellberg ; hellberg@chapman.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Real-time PCR ; ELISA ; Mislabeling ; Species identification ; Beef ; Pork
- 刊名：Food Control
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：71
- 期：Complete
- 页码：346-352
- 全文大小：261 K
文摘
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Real-time PCR detected beef consistently at 0.50%, compared to 1.00% for ELISA.
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Real-time PCR detected pork consistently at 0.10%, compared to 10.0% for ELISA.
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Compared to ELISA, real-time PCR showed greater agreement among duplicate samples.
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ELISA was found to be less time consuming and easier to perform than real-time PCR.
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ELISA and real-time PCR showed 100% specificity during reference sample testing.