Structural properties and release of insulin-loaded reverse hexagonal (HII) liquid crystalline mesophase

详细信息    查看全文

• 作者：Tehila Mishraki-Berkowitz¹ ; Abraham Aserin ; Nissim Garti ; ^{garti@mail.huji.ac.il" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Reverse hexagonal mesophase ; Glycerol monooleate ; Insulin ; Phosphatidylcholine ; Glycerol ; SAXS ; ATR-FTIR ; Rheology ; Release
• 刊名：Journal of Colloid and Interface Science
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：486
• 期：Complete
• 页码：184-193
• 全文大小：1437 K

文摘

Insulin loading into the H_II mesophases was examined as a function of its concentration, with addition of glycerol as a cosolvent and with addition of phosphatidylcholine (PC) as a structural stabilizer. The structural properties, the molecular interactions, the viscoelastic properties, and the dynamic behavior were investigated by SAXS, ATR-FTIR, and rheological measurements. Insulin release was then monitored and analyzed. Insulin incorporation into the H_II systems shrank the cylinders as it competed with the lipids in water-bonding. Insulin interrupted the interface while increasing τ_max and creating a more solid-like response. Upon addition of PC, cooperative flow behavior was detected, which is probably the reason for increase in insulin cumulative release from 28% to 52% after 300 min. In the presence of glycerol, the system was less cooperative but insulin was more compactly folded, resulting in a slight improvement in insulin release (up to 6%). Addition of both PC and glycerol caused the maximum release (55%). The addition of additives into the H_II system demonstrates how structural modifications can improve insulin release, and influence future design of encapsulated drug delivery systems.