RNA Sequence Context Effects Measured In Vitro Predict In Vivo Protein Binding and Regulation
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- 作者：J. Matthew Taliaferro1 ; 3 ; Nicole J. Lambert1 ; 3 ; Peter H. Sudmant1 ; Daniel Dominguez1 ; Jason J. Merkin1 ; Maria S. Alexis1 ; 2 ; Cassandra A. Bazile1 ; Christopher B. Burge1 ; 2 ; 4 ; cburge@mit.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：RBNS ; post-transcriptional ; RNA processing
- 刊名：Molecular Cell
- 出版年：2016
- 出版时间：20 October 2016
- 年：2016
- 卷：64
- 期：2
- 页码：294-306
- 全文大小：4788 K
文摘
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Most high-affinity RBP motifs in expressed RNAs are not bound in vivo
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A high-throughput in vitro interaction assay discriminates in vivo-bound motifs
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Motifs near ancient exons have reduced RNA structure and stronger RBP binding
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Deep mutagenesis reveals local RNA structure as a key binding determinant