Super RLuc8: A novel engineered Renilla luciferase with a red-shifted spectrum and stable light emission
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- 作者：Somaieh Rahnamaa ; Behnaz Saffarb ; Zahra Fanaei Kahranic ; Mahboobeh Nazarid ; Rahman Emamzadehc ; Sci_rahman@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; r.emamzadeh@sci.ui.ac.ir" class="auth_mail" title="E-mail the corresponding author
- 关键词：Renilla luciferase ; Enzyme kinetics ; Emission spectrum ; MD simulation ; Super RLuc8
- 刊名：Enzyme and Microbial Technology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：96
- 期：Complete
- 页码：60-66
- 全文大小：1409 K
文摘
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A novel engineered Renilla luciferase, super RLuc8 namely, was developed by directed evolution.
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Super RLuc8 has a red-shifted spectrum and shows stable light emission.
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The relationship between the protein structure, stability and emission spectrum has been studied in super RLuc8 using MD simulations.