Osteoblast-like cell mineralization induced by multiphasic calcium phosphate ceramic

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• 作者：Reed Ayers ; Sheila Nielsen-Preiss ; Virginia Ferguson ; Guglielmo Gotolli ; John J. Moore and Hans-Joachim Kleebe
• 刊名：Materials Science and Engineering: C
• 出版年：2006
• 出版时间：September 2006
• 年：2006
• 卷：26
• 期：8
• 页码：1333-1337
• 全文大小：370 K

文摘

The work presented here examines the effect of multiphasic CaP materials on the activity of SaOS-2 osteoblast like cells. Heterogeneous calcium phosphate (HCaP) was synthesized using self-propagating high temperature combustion synthesis (SHS). All samples were characterized using XRD, SEM, and FTIR. Cell culture procedures of HCaP with SaOS-2 cells followed ASTM 813-01. Samples were examined by Environmental SEM (ESEM) and EDS to characterize the mineral content of the cultures. Gold-coated samples were used for high-resolution imaging. Biomineralization was noted in unconditioned cultures exposed to HCaP while the controls (cells only in media and HCaP only in media) showed no mineralization. Calcium phosphate plate-like structures were identified adjacent to cells expressing calcium phosphate containing vesicles. These structures are similar to the organophosphate crystals seen in other published works. Plate-like crystals were noted in the larger membrane vesicles of SaOS-2 cells indicating the occurrence of cellularly mediated biomineralization.