Evaluation of different RNA extraction methods of filamentous fungi in various food matrices

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• 作者：Victoria Berná ; ldez ; Alicia Rodrí ; guez ; ^{aliciarj@unex.es} ; ; Mar Rodrí ; guez ; Lourdes Sá ; nchez-Montero ; Juan J. Có ; rdoba
• 关键词：RNA isolation ; Aflatoxins ; Mould
• 刊名：LWT - Food Science and Technology
• 出版年：2017
• 出版时间：May 2017
• 年：2017
• 卷：78
• 期：Complete
• 页码：47-53
• 全文大小：821 K
• 卷排序：78

文摘

Six mould RNA extraction protocols from contaminated foods were evaluated. Yield, quality, integrity and absence of genomic DNA were checked for all extracted RNA. The highest efficient method includes lysis using mortar and pestle and RNeasy kit.