Development of a M13 bacteriophage-based SPR detection using Salmonella as a case study
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- 作者：Nitsara Karoonuthaisiri ; Ratthaphol Charlermroj ; M. Josephine Morton ; Michalina Oplatowska-Stachowiak ; Irene R. Grant ; Christopher T. Elliott
- 关键词：Bacteriophage ; Peptide binders ; SPR ; Salmonella
- 刊名：Sensors and Actuators B: Chemical
- 出版年：January, 2014
- 年：2014
- 卷：190
- 期：Complete
- 页码：214-220
- 全文大小：1135 K
文摘
Surface plasmon resonance (SPR)-based biosensor is a popular platform for real-time monitoring and sensitive detection for a myriad of targets. However, only a few studies have reported the use of bacteriophages as specific binders for SPR-based detection. This study aimed to demonstrate how filamentous M13 bacteriophages expressing 12-mer peptides can be employed in an SPR-based assay, using a Salmonella-specific bacteriophage as a model binder to detect the foodborne bacterium Salmonella. Several important factors (immobilization buffers and methods, and interaction buffers) for a successful bacteriophage-based SPR assay were optimized. As a result, a Salmonella-specific bacteriophage-based SPR assay was achieved, with very low cross reactivity with other non-target foodborne pathogens and detection limits of 8.0 脳 107 and 1.3 脳 107 CFU/mL for one-time and five-time immobilized sensors, respectively. This proof-of-concept study demonstrates the feasibility of using M13 bacteriophages expressing target-specific peptides as a binder in a rapid and label-free SPR assay for pathogen detection.