Characterization of West Nile virus live vaccine candidates attenuated by capsid deletion mutations

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• 作者：Petra Schlick ; Regina M. Kofler ; Beate Schittl ; Christian Taucher ; Eszter Nagy ; Andreas Meinke ; Christian W. M ; l
• 关键词：West Nile virus ; Capsid deletion ; Mouse model
• 刊名：Vaccine
• 出版年：2010
• 出版时间：16 August 2010
• 年：2010
• 卷：28
• 期：36
• 页码：5903-5909
• 全文大小：216 K

文摘

Protein C deletion mutants of West Nile virus (WNV) were evaluated for their potential use as live virus vaccine candidates in vivo. Double and triple mutants carrying small deletions and second-site point mutations, as well as mutants with large deletions of 36 and 37 amino acid residues were tested in a stringent mouse challenge model. The mutant viruses were found to be non-pathogenic and to induce protective immunity in a wide range of inoculation doses (10¹–10⁶ FFU). Furthermore, the effects of combining three different previously identified resuscitating point mutations, as well as the combination of a large protein C deletion with a deletion mutation in the 3′ non-coding region were studied. The data indicate that the production of safe and efficacious WNV live vaccines based on protein C deletion mutations is feasible.