绵竹地震灾民集中安置点健康人群脑膜炎奈瑟菌带菌率调查
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摘要
目的了解汶川大地震后绵竹灾民集中安置点流行性脑膜炎奈瑟菌(Neisseria meningitides,Nm)带菌状况,为预测疫情和制定防治措施提供依据。方法对绵竹灾区二号桥灾民集中安置点和体育场灾民集中安置点进行单纯随机抽样,采取咽拭子样品887份,采用TaqMan-MGB探针RT-PCR技术以及试剂盒RT-PCR法进行检测。结果887份咽拭子检出3例阳性。结论灾区与四川省及全国其它地区往年带菌率相比流脑带菌率较低,发生流脑疫情的可能性不大,说明灾后卫生防疫等相关部实施的防御措施积极有效,但针对灾区实际环境,进一步的卫生防疫工作不可懈怠。
Objective To predict the possibility of epidemic outbreak of meningitis by testing Neissria Meningitides in a healthy population in the Mianzhu post-earthquake residential area. Methods A simple random sampling strategy was adopted to collect 887 throat swabs from a healthy population in the Mainzhu post-earthquake residential area. The TaqMan assay were performed to detect Neissria Meningitides. Results Three positive samples were identified. Conclusion The carrying rate of Neissria Meningitides is not high enough to bring about an epidemic outbreak of Meningitis. However,efforts to maintain a hygienic environment in the post-earthquake residential area should be continued.
Objective To predict the possibility of epidemic outbreak of meningitis by testing Neissria Meningitides in a healthy population in the Mianzhu post-earthquake residential area. Methods A simple random sampling strategy was adopted to collect 887 throat swabs from a healthy population in the Mainzhu post-earthquake residential area. The TaqMan assay were performed to detect Neissria Meningitides. Results Three positive samples were identified. Conclusion The carrying rate of Neissria Meningitides is not high enough to bring about an epidemic outbreak of Meningitis. However,efforts to maintain a hygienic environment in the post-earthquake residential area should be continued.
引文
1俞树荣主编.微生物学和微生物学检验.北京:人民卫生出版社,1997:155-158.
2蔡刚.实时定量PCR应用中的问题及优化方案.国外医学临床生物化学与检验学分册,2003;24(6):330-332.
3张晓春,朱昆蓉,龙海英等.嵌合荧光PCR法在调查成都市正常人群脑膜炎奈瑟氏菌携带率中的应用评价.现代预防医学,2009;36(9):1710-1712.
4石晓柳,李富春.射洪县健康人群评价水平和带菌率调查.预防医学情报杂志,2007;23(5):613-615.
5贾有菊.青海省健康人群脑膜炎奈瑟菌和抗体水平调查.中国疫苗和免疫,2008;14(5):460.
6吴兴华,林玫,李永红等.2006年广西流脑监测结果分析.应用预防医学,2008;14(3):161-163.
7林杰,郭维植,董新平等.PCR方法从脑脊液中检测脑膜炎奈瑟菌的实验.海峡预防医学杂志,2006;12(1):13-15.
8Landens JZ,Williams JN,Jones GR.Detection of meningococcal carriage by culture and PCR of throat swabs and mouth gargles.J Clin Microbiol,2002;40(1):75-79.
2蔡刚.实时定量PCR应用中的问题及优化方案.国外医学临床生物化学与检验学分册,2003;24(6):330-332.
3张晓春,朱昆蓉,龙海英等.嵌合荧光PCR法在调查成都市正常人群脑膜炎奈瑟氏菌携带率中的应用评价.现代预防医学,2009;36(9):1710-1712.
4石晓柳,李富春.射洪县健康人群评价水平和带菌率调查.预防医学情报杂志,2007;23(5):613-615.
5贾有菊.青海省健康人群脑膜炎奈瑟菌和抗体水平调查.中国疫苗和免疫,2008;14(5):460.
6吴兴华,林玫,李永红等.2006年广西流脑监测结果分析.应用预防医学,2008;14(3):161-163.
7林杰,郭维植,董新平等.PCR方法从脑脊液中检测脑膜炎奈瑟菌的实验.海峡预防医学杂志,2006;12(1):13-15.
8Landens JZ,Williams JN,Jones GR.Detection of meningococcal carriage by culture and PCR of throat swabs and mouth gargles.J Clin Microbiol,2002;40(1):75-79.
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