地震灾区某板房社区流脑带菌率调查
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摘要
[目的]了解汶川大地震后某板房社区内流行性脑膜炎奈瑟菌(Neisseria meningitides,Nm)带菌率状况和主要流行的血清群,为评价灾区卫生防疫效果和调整卫生防疫措施提供依据。[方法]对灾区某板房社区人群进行单纯随机抽样,采集咽拭子样品107份,采用传统的细菌学分离培养方法和分子生物学Taqman-MGB探针RT-PCR技术对流脑带菌率进行检测;对分离所得菌株进行血清学鉴定和PCR方法分群。[结果]对所采集的107份样品采用分子生物学Taqman-MGB探针RT-PCR技术检出3例阳性;细菌学分离培养得到3株阳性菌株,两种检测方法阳性样品编号一致,检测结果吻合,阳性率为2.80%。3株阳性菌株经血清学和分子生物学PCR方法分群,分别为B群Nm2株,C群Nm1株。[结论]与四川省流脑检测点及全国其他地区流脑带菌水平相比,社区流脑带菌率没有明显差别,流行仍以B群为主,但是流脑C群的出现应该引起足够的重视,防止流脑疫情的出现。
[Objective] To understand the Neissria meningitides-carrying rate and major epidemic serogroups in one prefab community of disaster area. To provide basis for evaluating the effects of epidemic prevention and adjustment of health and epidemic prevention measures for disaster area. [Methods] 107 throat swabs were collected from one prefab community of disaster area, used the traditional isolation and culture method and TaqMan assay for detection. The isolated strains were indentified by serotyping of and grouped by PCR. [Results] 3 positive samples were identified by the traditional method and TaqMan assay. Results were consistent with the two detection methods, the positive rate was 2.80%. 2 strains were Group B and 1 was Group C. [Conclusion] Comparing with epidemic cerebrospinal meningitis detection point in Sichuan Province and other parts of China, the germ-carrying rate is not high enough in the prefab community of disaster area. Group B is still the main epidemic flora. But we should pay attention to the emergence of Group C to prevent the outbreak of epidemic cerebrospinal meningitis.
[Objective] To understand the Neissria meningitides-carrying rate and major epidemic serogroups in one prefab community of disaster area. To provide basis for evaluating the effects of epidemic prevention and adjustment of health and epidemic prevention measures for disaster area. [Methods] 107 throat swabs were collected from one prefab community of disaster area, used the traditional isolation and culture method and TaqMan assay for detection. The isolated strains were indentified by serotyping of and grouped by PCR. [Results] 3 positive samples were identified by the traditional method and TaqMan assay. Results were consistent with the two detection methods, the positive rate was 2.80%. 2 strains were Group B and 1 was Group C. [Conclusion] Comparing with epidemic cerebrospinal meningitis detection point in Sichuan Province and other parts of China, the germ-carrying rate is not high enough in the prefab community of disaster area. Group B is still the main epidemic flora. But we should pay attention to the emergence of Group C to prevent the outbreak of epidemic cerebrospinal meningitis.
引文
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[2]蔡刚.实时定量PCR应用中的问题及优化方案[J].国外医学临床生物化学与检验学分册,2003,24(6):330-332.
[3]张晓春,朱昆蓉,龙海英,等.嵌合荧光PCR法在调查成都市正常人群脑膜炎奈瑟氏菌携带率中的应用评价[J].现代预防医学,2009,36(9):1710-1712.
[4]石晓柳,李富春.射洪县健康人群流脑抗体水平和带菌率调查[J].预防医学情报杂志,2007,23(5):613-615.
[5]贾有菊.青海省健康人群脑膜炎奈瑟菌和抗体水平调查[J].中国疫苗和免疫,2008,14(5):460.
[6]吴兴华,林玫,李永红,等.2006年广西流脑监测结果分析[J].应用预防医学,2008,14(3):161-163.
[7]俞树荣.微生物学和微生物学检验[M].北京:人民卫生出版社,1997.1552-1581.
[8]叶应妩,李健斋,王玉琛.临床实验诊断学[M].北京:人民卫生出版社,1994.1991-1992.
[9]余录根.2000年溧水县正常人群流脑带菌与免疫水平监测[J].预防医学情报杂志,2002,18(1):71-72.
[10]廖育煌,刘俊华,张欣强,等.广州1996～2003年健康人群流脑病原菌监测分析[J].华南预防医学,2004,30(4):291.
[11]刘丹清,王斌冰,陈霞,等.安徽省流行性脑脊髓膜炎流行病学分析及防制对策[J].中国计划免疫,2006,12(1):40-431.
[12]戴冰,倪进东,靳玉惠,等.合肥市流行性脑脊髓膜炎流行特征分析[J].中国公共卫生,2009,25(1):79-80.
[13]王隽,闫立群.2006年宁夏中卫市流行性脑脊髓膜炎监测结果分析[J].中国卫生检验杂志,2007,17(3):495.
[14]韩茂昌,陈德惠,宋光军,等.滁州市2004～2005年C群流脑流行特征分析[J].安徽预防医学杂志,2006,12(2):85-86.
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