Alleviation of Calcium on Toxicity of Cadmium to Rice Seedlings by Inhibiting Cadmium Accumulation and Relieving Oxidative Damage
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摘要
Accumulation characteristics and antioxidant enzyme activities in rice seedlings from a high-cadmium(Cd)-accumulating cultivar Tyou705(705)and low-Cd-accumulating cultivar Xiangzao 24(X24)were studied,based on hydroponic experiment.Three levels of calcium(Ca)(0,1.0 and 2.0 mmol·L~(-1))and two levels of Cd(0 and 2.7μmol·L~(-1))were designed in this study.Experimental results showed that the tolerance of rice seedlings to Cd stress was significantly improved by adding 1.0 or 2.0 mmol·L~(-1)Ca.Comparing with Cd control,adding 1.0 mmol·L~(-1) Ca made the seedling biomass of 705 and X24 increased by 68.9%and116.2%,respectively.Addition of 2.0 mmol·L~(-1) Ca was more effective than that of 1.0 mmol·L~(-1) Ca in promoting rice biomass,root tip number,total root length,and total root surface area.After addition of 2.0 mmol·L~(-1) Ca and Cd contents in roots and shoots of 705 decreased by 23.5%and 35.2%,Cd in roots and shoots of X24 decreased by 22.2%and 18.7%,respectively.Addition of Ca significantly promoted the accumulation of Mg,K and Zn in shoots and roots of X24 under Cd stress environment and eliminated the inhibitory effect of Cd on the uptake of these elements.Both 1.0 and 2.0 mmol·L~(-1) Ca significantly decreased the activities of superoxide dismutase(SOD)and peroxidase(POD)in rice seedlings under Cd stress.These results indicated that 1.0-2.0 mmol·L~(-1 )Ca could significantly reduce Cd content in rice roots and shoots,promote the uptake and accumulation of essential elements and alleviate the oxidative damage caused by Cd stress in rice seedlings.
Accumulation characteristics and antioxidant enzyme activities in rice seedlings from a high-cadmium(Cd)-accumulating cultivar Tyou705(705)and low-Cd-accumulating cultivar Xiangzao 24(X24)were studied,based on hydroponic experiment.Three levels of calcium(Ca)(0,1.0 and 2.0 mmol·L~(-1))and two levels of Cd(0 and 2.7μmol·L~(-1))were designed in this study.Experimental results showed that the tolerance of rice seedlings to Cd stress was significantly improved by adding 1.0 or 2.0 mmol·L~(-1)Ca.Comparing with Cd control,adding 1.0 mmol·L~(-1) Ca made the seedling biomass of 705 and X24 increased by 68.9%and116.2%,respectively.Addition of 2.0 mmol·L~(-1) Ca was more effective than that of 1.0 mmol·L~(-1) Ca in promoting rice biomass,root tip number,total root length,and total root surface area.After addition of 2.0 mmol·L~(-1) Ca and Cd contents in roots and shoots of 705 decreased by 23.5%and 35.2%,Cd in roots and shoots of X24 decreased by 22.2%and 18.7%,respectively.Addition of Ca significantly promoted the accumulation of Mg,K and Zn in shoots and roots of X24 under Cd stress environment and eliminated the inhibitory effect of Cd on the uptake of these elements.Both 1.0 and 2.0 mmol·L~(-1) Ca significantly decreased the activities of superoxide dismutase(SOD)and peroxidase(POD)in rice seedlings under Cd stress.These results indicated that 1.0-2.0 mmol·L~(-1 )Ca could significantly reduce Cd content in rice roots and shoots,promote the uptake and accumulation of essential elements and alleviate the oxidative damage caused by Cd stress in rice seedlings.
Accumulation characteristics and antioxidant enzyme activities in rice seedlings from a high-cadmium(Cd)-accumulating cultivar Tyou705(705)and low-Cd-accumulating cultivar Xiangzao 24(X24)were studied,based on hydroponic experiment.Three levels of calcium(Ca)(0,1.0 and 2.0 mmol·L~(-1))and two levels of Cd(0 and 2.7μmol·L~(-1))were designed in this study.Experimental results showed that the tolerance of rice seedlings to Cd stress was significantly improved by adding 1.0 or 2.0 mmol·L~(-1)Ca.Comparing with Cd control,adding 1.0 mmol·L~(-1) Ca made the seedling biomass of 705 and X24 increased by 68.9%and116.2%,respectively.Addition of 2.0 mmol·L~(-1) Ca was more effective than that of 1.0 mmol·L~(-1) Ca in promoting rice biomass,root tip number,total root length,and total root surface area.After addition of 2.0 mmol·L~(-1) Ca and Cd contents in roots and shoots of 705 decreased by 23.5%and 35.2%,Cd in roots and shoots of X24 decreased by 22.2%and 18.7%,respectively.Addition of Ca significantly promoted the accumulation of Mg,K and Zn in shoots and roots of X24 under Cd stress environment and eliminated the inhibitory effect of Cd on the uptake of these elements.Both 1.0 and 2.0 mmol·L~(-1) Ca significantly decreased the activities of superoxide dismutase(SOD)and peroxidase(POD)in rice seedlings under Cd stress.These results indicated that 1.0-2.0 mmol·L~(-1 )Ca could significantly reduce Cd content in rice roots and shoots,promote the uptake and accumulation of essential elements and alleviate the oxidative damage caused by Cd stress in rice seedlings.
引文
Ahmad P,Sarwat M,Bhat N A,et al.2015.Alleviation of cadmium toxicity in Brassica juncea L.(Czern.&Coss.)by calcium application involves various physiological and biochemical strategies.PLoSONE,10(1):e0114571.doi:10.1371/journal.pone.0114571
Basta N T,Ryan J A,Chaney R L.2005.Trace element chemistry in residual-treated soil:key concepts and metal bioavailability.Journal of Environmental Quality,34:49-63.
Belimov A A,Malkov N V,Puhalsky J V,et al.2018.The crucial role of roots in increased cadmium-tolerance and Cd-accumulation in the pea mutant SGECd.Biologia Plantarum,62(3):543-550.
Bush D S.1995.Calcium regulation in plant cells and its role in signaling.Annual Review of Plant Physiology Molecular Biology,46:95-112.
Chen R,Zhang C,Zhao Y,et al.2018.Foliar application with nanosilicon reduced cadmium accumulation in grains by inhibiting cadmium translocation in rice plants.Environ Sci Pollut Res,25:2361-2368.
Farooq M A,Detterbeck A,Clemens S,et al.2016.Silicon-induced reversibility of cadmium toxicity in rice.J Exp Bot,67:3573-3585.
Feng R W,Qiu W W,Lian F,et al.2013.Field evaluation of in situ remediation of Cd-contaminated soil using four additives,two foliar fertilisers and two varieties of pakchoi.Journal of Environmental Management,124:17-24.
Gong M,Chen S N,Song Y Q.1997.Effect of calcium and calmodulin on instrinsic heat tolerance in relation to antioxidant system in maize.Seedlings Australian Journal of Plant Physiology,24:371-379.
Hamilton D W A,Hills A,K?hler B,et al.2000.Ca2+channels at the plasma membrane of stomatal guard cells are activated by hyperpolarization and abscisic acid.Proceedings of the National Academy of Sciences USA,97:4967-4972.
Li P,Zhao C Z,Zhang Y Q,et al.2016.Calcium alleviates cadmiuminduced inhibition on root growth by maintaining auxin homeostasis in Arabidopsis seedlings.Protoplasma,253:185-200.DOI 10.1007/s00709-015-0810-9.
Li T Q,Yang X E,Lu L L,et al.2009.Effects of zinc and cadmium interactions on root morphology and metal translocation in a hyperaccumulating species under hydroponic conditions.Journal of Hazardous Materials,169:734-741.
Lux A,Martinka M,Vaculík M,et al.2011.Root responses to cadmium in the rhizosphere:a review.Journal of Experimental Botany,62:21-37.
Martin T A,Ruby M V.2004.Review of in situ remediation technologies for lead,zinc,and cadmium in soil.Remediation Journal,14:35-53.
Pei Z M,Murata Y,Benning G,et al.2000.Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells.Nature,406:731-734.
Perfus-Barbeoch L,Leonhardt N,Vavasseur A,et al.2002.Heavy metal toxicity:cadmium permeates through calcium channels and disturbs the plant water status.The Plant Journal,32:539-548.
Pinto E,Ferreira I M.2015.Cation transporters/channels in plants:tools for nutrient biofortification.J Plant Physiol,179:64-82.
Rodríguez-Serrano M,Romero-Puertas M C,Zabalza A,et al.2006.Cadmium effect on oxidative metabolism of pea(Pisum sativum L.)roots.Imaging of reactive oxygen species and nitric oxide accumulation in vivo.Plant Cell and Environment,29:1532-1544.
Sarwar N,Saifullah M S S,Zia M H,et al.2010.Role of mineral nutrition in minimizing cadmium accumulation by plants.Sci Food Agric,90:925-937.
StaňováA,?uri?ováE,BanásováV,et al.2012.Root system morphology and primary root anatomy in natural non-metallicolous and metallicolous populations of three Arabidopsis species differing in heavy metal tolerance.Biologia,67:505-516.
Wang S,Wang F,Gao S.2015.Foliar application with nano-silicon alleviates Cd toxicity in rice seedlings.Environ Sci Pollut Res,22:2837-2845.DOI 10.1007/s11356-014-3525-0.
Wei S H,Li Y M,Zhan J,et al.2012.Tolerant mechanisms of Rorippa globosa(Turcz.)Thell.hyperaccumulating Cd explored from root morphology.Bioresource Technology,118:455-459.
Zeng H,Xu L,Amarjeet S,et al.2015.Involvement of calmodulin and calmodulin-like proteins in plant responses to abiotic stresses.Frontiers in Plant Science,6:600.
Zhang L,Chen Z,Zhu C.2012.Endogenous nitric oxide mediates alleviation of cadmium toxicity induced by calcium in rice seedlings.Journal of Environmental Sciences,24:940-948.
Zhao Y,Zhang S,Wen N,et al.2017.Modeling uptake of cadmium from solution outside of root to cell wall of shoot in rice seedling.Plant Growth Regulation,82:11-20.
Basta N T,Ryan J A,Chaney R L.2005.Trace element chemistry in residual-treated soil:key concepts and metal bioavailability.Journal of Environmental Quality,34:49-63.
Belimov A A,Malkov N V,Puhalsky J V,et al.2018.The crucial role of roots in increased cadmium-tolerance and Cd-accumulation in the pea mutant SGECd.Biologia Plantarum,62(3):543-550.
Bush D S.1995.Calcium regulation in plant cells and its role in signaling.Annual Review of Plant Physiology Molecular Biology,46:95-112.
Chen R,Zhang C,Zhao Y,et al.2018.Foliar application with nanosilicon reduced cadmium accumulation in grains by inhibiting cadmium translocation in rice plants.Environ Sci Pollut Res,25:2361-2368.
Farooq M A,Detterbeck A,Clemens S,et al.2016.Silicon-induced reversibility of cadmium toxicity in rice.J Exp Bot,67:3573-3585.
Feng R W,Qiu W W,Lian F,et al.2013.Field evaluation of in situ remediation of Cd-contaminated soil using four additives,two foliar fertilisers and two varieties of pakchoi.Journal of Environmental Management,124:17-24.
Gong M,Chen S N,Song Y Q.1997.Effect of calcium and calmodulin on instrinsic heat tolerance in relation to antioxidant system in maize.Seedlings Australian Journal of Plant Physiology,24:371-379.
Hamilton D W A,Hills A,K?hler B,et al.2000.Ca2+channels at the plasma membrane of stomatal guard cells are activated by hyperpolarization and abscisic acid.Proceedings of the National Academy of Sciences USA,97:4967-4972.
Li P,Zhao C Z,Zhang Y Q,et al.2016.Calcium alleviates cadmiuminduced inhibition on root growth by maintaining auxin homeostasis in Arabidopsis seedlings.Protoplasma,253:185-200.DOI 10.1007/s00709-015-0810-9.
Li T Q,Yang X E,Lu L L,et al.2009.Effects of zinc and cadmium interactions on root morphology and metal translocation in a hyperaccumulating species under hydroponic conditions.Journal of Hazardous Materials,169:734-741.
Lux A,Martinka M,Vaculík M,et al.2011.Root responses to cadmium in the rhizosphere:a review.Journal of Experimental Botany,62:21-37.
Martin T A,Ruby M V.2004.Review of in situ remediation technologies for lead,zinc,and cadmium in soil.Remediation Journal,14:35-53.
Pei Z M,Murata Y,Benning G,et al.2000.Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells.Nature,406:731-734.
Perfus-Barbeoch L,Leonhardt N,Vavasseur A,et al.2002.Heavy metal toxicity:cadmium permeates through calcium channels and disturbs the plant water status.The Plant Journal,32:539-548.
Pinto E,Ferreira I M.2015.Cation transporters/channels in plants:tools for nutrient biofortification.J Plant Physiol,179:64-82.
Rodríguez-Serrano M,Romero-Puertas M C,Zabalza A,et al.2006.Cadmium effect on oxidative metabolism of pea(Pisum sativum L.)roots.Imaging of reactive oxygen species and nitric oxide accumulation in vivo.Plant Cell and Environment,29:1532-1544.
Sarwar N,Saifullah M S S,Zia M H,et al.2010.Role of mineral nutrition in minimizing cadmium accumulation by plants.Sci Food Agric,90:925-937.
StaňováA,?uri?ováE,BanásováV,et al.2012.Root system morphology and primary root anatomy in natural non-metallicolous and metallicolous populations of three Arabidopsis species differing in heavy metal tolerance.Biologia,67:505-516.
Wang S,Wang F,Gao S.2015.Foliar application with nano-silicon alleviates Cd toxicity in rice seedlings.Environ Sci Pollut Res,22:2837-2845.DOI 10.1007/s11356-014-3525-0.
Wei S H,Li Y M,Zhan J,et al.2012.Tolerant mechanisms of Rorippa globosa(Turcz.)Thell.hyperaccumulating Cd explored from root morphology.Bioresource Technology,118:455-459.
Zeng H,Xu L,Amarjeet S,et al.2015.Involvement of calmodulin and calmodulin-like proteins in plant responses to abiotic stresses.Frontiers in Plant Science,6:600.
Zhang L,Chen Z,Zhu C.2012.Endogenous nitric oxide mediates alleviation of cadmium toxicity induced by calcium in rice seedlings.Journal of Environmental Sciences,24:940-948.
Zhao Y,Zhang S,Wen N,et al.2017.Modeling uptake of cadmium from solution outside of root to cell wall of shoot in rice seedling.Plant Growth Regulation,82:11-20.
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