高、低糖甘蔗品种成熟期糖分积累特征及代谢相关酶活性分析
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摘要
为分析高、低糖甘蔗品种蔗糖代谢相关酶与糖分积累的相关性和差异性,对甘蔗高糖(GT35)和低糖(B8)品种成熟期不同成熟度的叶和茎中的蔗糖合成和分解方向酶活性、蔗糖、葡萄糖和果糖含量进行分析。结果表明,2个甘蔗品种叶中己糖含量与NI酶活性显著负相关,茎中蔗糖含量与SPS和CIN酶活性显著正相关,而与SS-s、SS-c、SAI和NI酶活性显著负相关。在GT35成熟茎和老茎中蔗糖含量显著高于B8,己糖含量则显著低于B8。GT35成熟叶、成熟茎和老茎中SS-s酶活性显著高于B8,高SS-s酶活性有利于蔗糖合成。茎中SS-c、SAI和NI酶活性由高到低依次为幼茎、成熟茎和老茎,分解酶活性降低有利于甘蔗茎间蔗糖积累。而随着节间成熟,CIN酶活性提高,有利于茎间蔗糖运输和积累。基于以上结果,认为叶中高SS-s和SPS酶活性,茎中高SPS、SS-s和CIN酶活性,低SAI、SS-c和NI酶活性,可能是高糖甘蔗品种成熟期茎中蔗糖积累的重要调节因素。
To analyze the correlation and difference of sucrose metabolic enzymes and sucrose accumulation in high and low sugar sugarcane,the experiment was conducted with the split plot to analyze the sucrose synthesis and decomposition direction enzyme activity,sucrose,glucose and fructose contents in leaves and stems of different maturity at the maturing stage of high sugar(GT35)and low sugar(B8)sugarcane.The results showed that in the GT35 and B8 the content of sucrose in the leaves was positively correlated with the activity of neutral/alkaline invertase(NI),the sucrose content in the stem was positively correlated with the activity of sucrose phosphate synthase(SPS)and cell wall-bound invertase(CIN),and negatively correlated with the activity of the sucrose synthase in the synthesis direction(SS-s),the sucrose synthetase in the cleavage direction(SS-c),soluble acid invertase(SAI)and NI.The sucrose content in the mature and matured stems of GT35 was significantly higher than that of B8,while the hexose content was significantly lower than that of B8.During the sugarcane maturing stage,the activity of SS-s in the mature leaves,mature stems and matured stems of GT35 was significantly higher than that of B8,and high SS-s activity was beneficial to sucrose synthesis.The activities in SS-c,SAI and NI in sugarcane stems were in the order of immature stem >maturing stem > matured stem,and its enzyme activity reduced to facilitate the sucrose accumulation in the stem.With the increase of CIN enzyme activity during the mafuring period of the internode,it is beneficial to the transportation and accumulation of sucrose in sugarcane stems.The analysis demonstrated that high SS-s and SPS activities in leaves,high SPS,SS-s and CIN activities in stems,and low SAI,SS-c and NI activities in stems may be important factors for regulating sucrose accumulation in high sugar varieties of sugarcane at the maturing stage.
To analyze the correlation and difference of sucrose metabolic enzymes and sucrose accumulation in high and low sugar sugarcane,the experiment was conducted with the split plot to analyze the sucrose synthesis and decomposition direction enzyme activity,sucrose,glucose and fructose contents in leaves and stems of different maturity at the maturing stage of high sugar(GT35)and low sugar(B8)sugarcane.The results showed that in the GT35 and B8 the content of sucrose in the leaves was positively correlated with the activity of neutral/alkaline invertase(NI),the sucrose content in the stem was positively correlated with the activity of sucrose phosphate synthase(SPS)and cell wall-bound invertase(CIN),and negatively correlated with the activity of the sucrose synthase in the synthesis direction(SS-s),the sucrose synthetase in the cleavage direction(SS-c),soluble acid invertase(SAI)and NI.The sucrose content in the mature and matured stems of GT35 was significantly higher than that of B8,while the hexose content was significantly lower than that of B8.During the sugarcane maturing stage,the activity of SS-s in the mature leaves,mature stems and matured stems of GT35 was significantly higher than that of B8,and high SS-s activity was beneficial to sucrose synthesis.The activities in SS-c,SAI and NI in sugarcane stems were in the order of immature stem >maturing stem > matured stem,and its enzyme activity reduced to facilitate the sucrose accumulation in the stem.With the increase of CIN enzyme activity during the mafuring period of the internode,it is beneficial to the transportation and accumulation of sucrose in sugarcane stems.The analysis demonstrated that high SS-s and SPS activities in leaves,high SPS,SS-s and CIN activities in stems,and low SAI,SS-c and NI activities in stems may be important factors for regulating sucrose accumulation in high sugar varieties of sugarcane at the maturing stage.
引文
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[11]Szarka A,Horemans N,Passarella S,et al.Demonstration of an intramitochondrial invertase activity and the corresponding sugar transporters of the inner mitochondrial membrane in Jerusalem artichoke(Helianthus tuberosus L.)tubers[J].Planta,2008,228(5):765-775.
[12]Murayama S,Handa H.Genes for alkaline/neutral invertase in rice:alkaline/neutral invertases are located in plant mitochondria and also in plastids[J].Planta,2007,225(5):1193-1203.
[13]闫梅玲,代红军,单守明,等.蔗糖代谢相关酶对果实糖积累影响的研究进展[J].安徽农业科学,2009,37(29):14021-14023.Yan M L,Dai H J,Shan S M,et al.Research progress of the influence of sucrose-metabolizing enzymes on the sugar accumulationin fruit[J].Journal of Anhui Agricultural Sciences,2009,37(29):14021-14023.
[14]牛俊奇,Phan Thi Thu,邵敏,等.甘蔗工艺成熟期转化酶及其抑制子与蔗糖积累的相关性研究[J].西南农业学报,2015,28(4):1606-1611.Niu J Q,Phan T T,Shao M.Correlation analysis among invertases activities,invertase inhibitor gene expression and sugar content at technical maturing stage of sugarcane[J].Southwest China Journal of Agricultural Sciences,2015,28(4):1606-1611.
[15]牛俊奇,黄静丽,赵文慧,等.甘蔗工艺成熟期SS和SPS酶活性与糖分积累的相关性研究[J].生物技术通报,2015,31(9):105-110.Niu J Q,Huang J L,Zhao W H.Correlations between sucrose accumulation and activities of SPS,SS and processing maturing stage of sugarcane[J].Biotechnology Bulletin,2015,31(9):105-110.
[16]罗海玲,潘有强,李杨瑞.不同甘蔗品种的含糖特性及成熟期分析[J].广西农业科学,2006,37(3):245-248.Luo H L,Pan Y Q,Li Y R.Analysis of sucrose quality and maturing stage on dif ferent sugarcane varieties[J].Guangxi Agricultural Sciences,2006,37(3):245-248.
[17]李丹,王晔,赫磊,等.甜菜蔗糖代谢相关酶活性与糖积累关系的研究[J].作物杂志,2009(3):27-31.Li D,Wang Y,He L,et al.Relaitonship between sucrose meatbolisim relaetd enzymes and sucrose accumulation in Sugar beet[J].Corp,2009(3):27-31.
[18]陈兰平,陈由强,方静平,等.甘蔗SPS基因家族成员表达与糖分积累关系解析[J].热带作物学报,2014,35(7):1354-1361.Chen L P,Chen Y Q,Fang J P,et al.Analysis of relation between SPS gene family expression and sugar accumulation in sugarcane[J].Chinese Journal of Tropical Crops,2014,35(7):1354-1361.
[19]Huang D L,Qin C X,Gui Y Y,et al.Role of the SPS,gene families in the regulation of sucrose accumulation in sugarcane[J].Sugar Tech,2016,19(2):1-8.
[20]Verma A K,Upadhyay S K,Verma P C,et al.Functional analysis of sucrose phosphate synthase(SPS)and sucrose synthase(SS)in sugarcane(Saccharum)cultivars[J].Plant Biology,2011,13(2):325-332.
[21]景红霞,章英才,陈亚萍,等.糖代谢相关酶在灵武长枣叶片和果柄糖积累中的作用[J].西北植物学报,2014,34(9):1821-1827.Jing H X,Zhang Y C,Chen Y P.Roles of sucrose-metabolizing enzymes in sugar accumulation in leaves and carpopodiums of Ziziphus jujuba Mill. cv. Lingwuchangzao[J].Acta Botanica Boreali-Occidentalia Sinica,2014,34(9):1821-1827.
[22]章英才,陈亚萍,景红霞,等.‘灵武长枣’果实糖积累与蔗糖代谢相关酶的关系[J].果树学报,2014,31(2):250-257.Zhang Y C,Chen Y P,Jing H X,et al.Relation between sugar accumulation and sucrose-metabolizing enzymes in fruit of‘Lingwuchangzao’(Zizyphus jujuba Mill.)[J].Journal of Fruit Science,2014,31(2):250-257.
[23]Pan Y Q,Lou H L,Li Y R.Soluble acid invertase and sucrose phosphate synthase:Key enzymes in regulating sucrose accumulation in sugarcane stalk[J].Sugar Tech,2009,11(1):28-33.
[24]Liu Y,Offler C E,Ruan Y L.Cell wall invertase promotes fruit set under heat stress by suppressing ROS-independent plant cell death[J].Plant Physiology,2016,172(1):1-6.
[25]牛俊奇,苗小荣,王露蓉,等.甘蔗细胞壁转化酶基因(SoCIN1)克隆及其在转基因烟草中的表达特性分析[J].南方农业学报,2017,48(10):1727-1733.Niu J Q,Miao X R,Wang L R,et al.Cloning of cell wall invertase gene(SoCIN1)in sugarcane and its expression characteristics in transgenic tobacco[J].Journal of Southern Agriculture,2017,48(10):1727-1733.
[26]Heyer A G,Raap M,Schroeer B,et al.Cell wall invertase expression at the apical meristem alters floral,architectural,and reproductive traits in Arabidopsis thaliana[J].Plant Journal,2004,39(2):161-169.
[27]Kocal N,Sonnewald U,Sonnewald S.Cell wall-bound invertase limits sucrose export and is involved in symptom development and inhibition of photosynthesis during compatible interaction between tomato and Xanthomonas campestris pv vesicatoria[J].Plant Physiology,2008,148(3):1523-1536.
[28]Tang X,Su T,Han M,et al.Suppression of extracellular invertase inhibitor gene expression improves seed weight in soybean(Glycine max)[J].Journal of Experimental Botany,2017,68(3):469-482.
[29]周文灵,江永,李奇伟,等.甘蔗蔗糖积累的规律、影响因素及其调控机制的研究进展[J].甘蔗糖业,2011(6):11-17.Zhou W L,Jiang Y,Li J W,et al.Advances in the rules,influencing factors and regulatory mechanisms of sucrose accumulation in sugarcane[J].Sugarcane Canesugar,2011(6):11-17.
[2]Uys L,Botha F C,Hofmeyr J H,et al.Kinetic model of sucrose accumulation in maturing sugarcane culm tissue[J].Phytochemistry,2007,68(16/18):2375-2392.
[3]Mccormick A J,Watt D A,Cramer M D. Supply and demand:sink regulation of sugar accumulation in sugarcane[J].Journal of Experimental Botany,2009,60(2):357-364.
[4]赵丽宏,王俊刚,杨本鹏,等.甘蔗体内的蔗糖积累[J].基因组学与应用生物学,2009,28(2):385-390.Zhao L H,Wang J G,Yang B P,et al.Sucrose accumulation in the sugarcane[J].Genomics Applied Biology,2009,28(2):385-390.
[5]黄东亮,李双喜,廖青,等.植物蔗糖磷酸合成酶研究进展[J].中国生物工程杂志,2012,32(6):109-119.Huang D L,Li S X,Liao Q,et al.Advances on sucrose phosphate synthase in plants[J].China Biotechnology,2012,32(6):109-119.
[6]Li X,Du J,Guo J,et al.The functions of cucumber sucrose phosphate synthases 4(CsSPS4)in carbon metabolism and transport in sucrose-and stachyose-transporting plants[J].Journal of Plant Physiology,2018,228:150-157.
[7]房经贵,朱旭东,贾海锋,等.植物蔗糖合酶生理功能研究进展[J].南京农业大学学报,2017,40(5):759-768.Fang J G,Zhu X D,Jia H F,et al.Research advances on physiological function of plant sucrose synthase[J].Journal of Nanjing Agricultural University,2017,40(5):759-768.
[8]俞锞,李志邈,万红建,等.高等植物蔗糖转化酶功能的研究进展[J].安徽农业科学,2013,41(33):12815-12818.Yu K,Li Z M,Wang H J,et al.Research advances on the function of invertase in higher plants[J].Journal of Anhui Agricultural Sciences,2013,41(33):12815-12818.
[9]Niu J Q,Wang A Q,Huang J L,et al.Isolation,Characterization and promoter analysis of cell wall invertase gene SoCIN1from sugarcane(Saccharum spp.)[J].Sugar Tech,2015,17(1):65-76.
[10]赵杰堂.蔗糖转化酶在高等植物生长发育及胁迫响应中的功能研究进展[J].热带亚热带植物学报,2016,24(3):352-358.Zhao J T.Advances in research on invertase in plant development and response to abiotic and biotic stresses[J].Journal of Tropical and Subtropical Botany,2016,24(3):352-358.
[11]Szarka A,Horemans N,Passarella S,et al.Demonstration of an intramitochondrial invertase activity and the corresponding sugar transporters of the inner mitochondrial membrane in Jerusalem artichoke(Helianthus tuberosus L.)tubers[J].Planta,2008,228(5):765-775.
[12]Murayama S,Handa H.Genes for alkaline/neutral invertase in rice:alkaline/neutral invertases are located in plant mitochondria and also in plastids[J].Planta,2007,225(5):1193-1203.
[13]闫梅玲,代红军,单守明,等.蔗糖代谢相关酶对果实糖积累影响的研究进展[J].安徽农业科学,2009,37(29):14021-14023.Yan M L,Dai H J,Shan S M,et al.Research progress of the influence of sucrose-metabolizing enzymes on the sugar accumulationin fruit[J].Journal of Anhui Agricultural Sciences,2009,37(29):14021-14023.
[14]牛俊奇,Phan Thi Thu,邵敏,等.甘蔗工艺成熟期转化酶及其抑制子与蔗糖积累的相关性研究[J].西南农业学报,2015,28(4):1606-1611.Niu J Q,Phan T T,Shao M.Correlation analysis among invertases activities,invertase inhibitor gene expression and sugar content at technical maturing stage of sugarcane[J].Southwest China Journal of Agricultural Sciences,2015,28(4):1606-1611.
[15]牛俊奇,黄静丽,赵文慧,等.甘蔗工艺成熟期SS和SPS酶活性与糖分积累的相关性研究[J].生物技术通报,2015,31(9):105-110.Niu J Q,Huang J L,Zhao W H.Correlations between sucrose accumulation and activities of SPS,SS and processing maturing stage of sugarcane[J].Biotechnology Bulletin,2015,31(9):105-110.
[16]罗海玲,潘有强,李杨瑞.不同甘蔗品种的含糖特性及成熟期分析[J].广西农业科学,2006,37(3):245-248.Luo H L,Pan Y Q,Li Y R.Analysis of sucrose quality and maturing stage on dif ferent sugarcane varieties[J].Guangxi Agricultural Sciences,2006,37(3):245-248.
[17]李丹,王晔,赫磊,等.甜菜蔗糖代谢相关酶活性与糖积累关系的研究[J].作物杂志,2009(3):27-31.Li D,Wang Y,He L,et al.Relaitonship between sucrose meatbolisim relaetd enzymes and sucrose accumulation in Sugar beet[J].Corp,2009(3):27-31.
[18]陈兰平,陈由强,方静平,等.甘蔗SPS基因家族成员表达与糖分积累关系解析[J].热带作物学报,2014,35(7):1354-1361.Chen L P,Chen Y Q,Fang J P,et al.Analysis of relation between SPS gene family expression and sugar accumulation in sugarcane[J].Chinese Journal of Tropical Crops,2014,35(7):1354-1361.
[19]Huang D L,Qin C X,Gui Y Y,et al.Role of the SPS,gene families in the regulation of sucrose accumulation in sugarcane[J].Sugar Tech,2016,19(2):1-8.
[20]Verma A K,Upadhyay S K,Verma P C,et al.Functional analysis of sucrose phosphate synthase(SPS)and sucrose synthase(SS)in sugarcane(Saccharum)cultivars[J].Plant Biology,2011,13(2):325-332.
[21]景红霞,章英才,陈亚萍,等.糖代谢相关酶在灵武长枣叶片和果柄糖积累中的作用[J].西北植物学报,2014,34(9):1821-1827.Jing H X,Zhang Y C,Chen Y P.Roles of sucrose-metabolizing enzymes in sugar accumulation in leaves and carpopodiums of Ziziphus jujuba Mill. cv. Lingwuchangzao[J].Acta Botanica Boreali-Occidentalia Sinica,2014,34(9):1821-1827.
[22]章英才,陈亚萍,景红霞,等.‘灵武长枣’果实糖积累与蔗糖代谢相关酶的关系[J].果树学报,2014,31(2):250-257.Zhang Y C,Chen Y P,Jing H X,et al.Relation between sugar accumulation and sucrose-metabolizing enzymes in fruit of‘Lingwuchangzao’(Zizyphus jujuba Mill.)[J].Journal of Fruit Science,2014,31(2):250-257.
[23]Pan Y Q,Lou H L,Li Y R.Soluble acid invertase and sucrose phosphate synthase:Key enzymes in regulating sucrose accumulation in sugarcane stalk[J].Sugar Tech,2009,11(1):28-33.
[24]Liu Y,Offler C E,Ruan Y L.Cell wall invertase promotes fruit set under heat stress by suppressing ROS-independent plant cell death[J].Plant Physiology,2016,172(1):1-6.
[25]牛俊奇,苗小荣,王露蓉,等.甘蔗细胞壁转化酶基因(SoCIN1)克隆及其在转基因烟草中的表达特性分析[J].南方农业学报,2017,48(10):1727-1733.Niu J Q,Miao X R,Wang L R,et al.Cloning of cell wall invertase gene(SoCIN1)in sugarcane and its expression characteristics in transgenic tobacco[J].Journal of Southern Agriculture,2017,48(10):1727-1733.
[26]Heyer A G,Raap M,Schroeer B,et al.Cell wall invertase expression at the apical meristem alters floral,architectural,and reproductive traits in Arabidopsis thaliana[J].Plant Journal,2004,39(2):161-169.
[27]Kocal N,Sonnewald U,Sonnewald S.Cell wall-bound invertase limits sucrose export and is involved in symptom development and inhibition of photosynthesis during compatible interaction between tomato and Xanthomonas campestris pv vesicatoria[J].Plant Physiology,2008,148(3):1523-1536.
[28]Tang X,Su T,Han M,et al.Suppression of extracellular invertase inhibitor gene expression improves seed weight in soybean(Glycine max)[J].Journal of Experimental Botany,2017,68(3):469-482.
[29]周文灵,江永,李奇伟,等.甘蔗蔗糖积累的规律、影响因素及其调控机制的研究进展[J].甘蔗糖业,2011(6):11-17.Zhou W L,Jiang Y,Li J W,et al.Advances in the rules,influencing factors and regulatory mechanisms of sucrose accumulation in sugarcane[J].Sugarcane Canesugar,2011(6):11-17.
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