库区植物塘水生植物附着细菌群落季节演替特征及影响因素
|
摘要
采集天然湿地中的狐尾藻、凤眼莲、睡莲3种水生植物,采用高通量测序分析3种水生植物表面附着细菌群落,探究其细菌群落结构的季节演替,并分析环境因子对群落变化的影响。结果表明:(a)3种水生植物附着细菌主要有蓝细菌门(Cyanobacteria)、变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria),这3种植物表面附着的细菌群落结构在门水平上类似,在属水平上丰富度及演替特征存在差异,主要与植物茎叶面积、植物分泌物质及植物茎叶光合作用强度等因素有关;(b)在属水平上,这3种水生植物附着细菌群落季节演替是具有一定方向性的自发进行的过程,不受外在单一因子的限制,是宿主植物和外部水环境共同作用的结果;(c)通过聚类分析发现,环境变化对附着细菌群落结构的影响程度高于植物种类,环境因子的CCA分析表明温度、pH、TN是水生植物附着细菌群落演替的主要影响因素。
Three aquatic plants, Foxtail algae, Hyacinth and Water lily, were collected from natural wetlands, and the bacterial community attached to the surface of each plant was analyzed by the high-throughput sequencing. The seasonal succession of bacterial community structure was explored, and the influence of environmental factors on the change of community was also analyzed. The results show that:(a) Cyanobacteria, Proteobacteria, Bacteroidetes and Actinobacteria were the main bacteria adhering to these aquatic plants. The community structure of bacteria adhering to the surface of the three aquatic plants was similar at the phylum level, while the abundance and succession characteristics were different at the generic level, which are mainly related to the plant stem and leaf area, the plant secretion and the photosynthetic intensity.(b) At the generic level, the seasonal succession of bacterial community attached to three aquatic plants is a spontaneous process with a certain direction, which is not limited by the external single factor and is the affected result of host plant and external water environment.(c) Through the cluster analysis, it was found that the effect of environmental change on the community structure of attached bacteria was higher than that of plant species. CCA analysis of environmental factors showed that temperature, pH and TN were the main factors affecting the succession of attached bacterial community of aquatic plants.
Three aquatic plants, Foxtail algae, Hyacinth and Water lily, were collected from natural wetlands, and the bacterial community attached to the surface of each plant was analyzed by the high-throughput sequencing. The seasonal succession of bacterial community structure was explored, and the influence of environmental factors on the change of community was also analyzed. The results show that:(a) Cyanobacteria, Proteobacteria, Bacteroidetes and Actinobacteria were the main bacteria adhering to these aquatic plants. The community structure of bacteria adhering to the surface of the three aquatic plants was similar at the phylum level, while the abundance and succession characteristics were different at the generic level, which are mainly related to the plant stem and leaf area, the plant secretion and the photosynthetic intensity.(b) At the generic level, the seasonal succession of bacterial community attached to three aquatic plants is a spontaneous process with a certain direction, which is not limited by the external single factor and is the affected result of host plant and external water environment.(c) Through the cluster analysis, it was found that the effect of environmental change on the community structure of attached bacteria was higher than that of plant species. CCA analysis of environmental factors showed that temperature, pH and TN were the main factors affecting the succession of attached bacterial community of aquatic plants.
引文
[1]何聃,任丽娟,邢鹏,等.沉水植物附着细菌群落结构及其多样性研究进展[J].生命科学,2014,26(2):161-168.(HEDan,REN Lijuan,XING Peng,et al.Research progress of diversity and community structure of epiphytic bacteria on submerged macrophytes[J].Chinese Bulletin of Life Sciences,2014,26(2):161-168.(in Chinese))
[2]刘凯辉,张松贺,吕小央,等.南京花神湖3种沉水植物表面附着微生物群落特征[J].湖泊科学,2015,27(1):103-112.(LIU Kaihui,ZHANG Songhe,LYU Xiaoyang,et al.The characteristics of epiphytic microbes of three submerged macrophytes in Lake Huashen[J].Journal of Lake Sciences,2015,27(1):103-112.(in Chinese))
[3]HE D,REN L,WU Q.Epiphytic bacterial communities on two common submerged macrophytes in Taihu Lake:diversity and host-specificity[J].Chinese Journal of Oceanology and Limnology,2012,30(2):237-247.
[4]PANG S,ZHANG S,LYU X Y,et al.Characterization of bacterial community in biofilm and sediments of wetlands dominated by aquatic macrophytes[J].Ecological Engineering,2016,97:242-250.
[5]马杰.浅水湖泊典型沉水植物附着细菌群落结构特征[D].南京:南京师范大学,2016.
[6]POLLARD P C.Bacterial activity in plant(Schoenoplectus validus)biofilms of constructed wetlands[J].Water Research,2010,44(20):5939-5948.
[7]马杰,范婤,王国祥,等.洪泽湖3种沉水植物附着细菌群落结构及多样性[J].湖泊科学,2016,28(4):852-858.(MA Jie,FAN Zhou,WANG Guoxiang,et al.Diversity and community structure of epiphytic bacteria on different submerged macrophytes in Lake Hongze[J].Journal of Lake Sciences,2016,28(4):852-858.(in Chinese))
[8]由文辉.淀山湖着生藻类群落结构与数量特征[J].环境科学,1999,20(5):62-65.(YOU Wenhui.The species composition and quantitative features of the periphytic algae communities in Dianshan Lake[J].Environmental Science,1999,20(5):62-65.(in Chinese))
[9]常会庆,丁学峰,蔡景波.水生植物分泌物对微生物影响的研究[J].水土保持研究,2007,14(4):57-60.(CHANGHuiqing,DING Xuefeng,CAI Jingbo.Eeffects of crude exudates of a aquatic macrophytes on bacteria[J].Reasearch of Soil and Water Conservation,2007,14(4):57-60.(in Chinese))
[10]董彬,韩睿明,王国祥.沉水植物茎叶微界面特性研究进展[J].生态学报,2017,37(6):1769-1776.(DONG Bin,HANRuiming,WANG Guoxiang.Research advances in and perspectives on characteristics of the micro-boundary layer around leaves and stems of submerged macrophytes[J].Acta Ecologica Sinica,2017,37(6):1769-1776.(in Chinese))
[11]RAEDER U,RUZICKA J,GOOS C.Characterization of the light attenuation by periphyton in lakes of different trophic state[J].Limnologica,2010,40(1):40-46.
[12]杨飞,尹杰,张毅敏,等.两种沉水植物附着生物种群特征对水深的响应研究[J].环境科学学报,2018,38(6):1-14.(YANG Fei,YIN Jie,ZHANG Yimin,et al.Research on characteristics of epiphytic organism of two submerged macrophytes and its response to water depth[J].Acta Scientiae Circumstantiae,2018,38(6):1-14.(in Chinese))
[13]RUSCONI R,LECUYER S,AUTRUSSON N,et al.Secondary flow as a mechanism for the formation of biofilm streamers[J].Biophysical Journal,2011,100(12):3054.
[14]SANDJENSEN K,REVSBECH N P,RGENSEN B J.Microprofiles of oxygen in epiphyte communities on submerged macrophytes[J].Marine Biology,1985,89(1):55-62.
[15]宋玉芝,秦伯强,高光.附着生物对富营养化水体氮磷的去除效果[J].长江流域资源与环境,2009,18(2):180-185.(SONG Yuzhi,QIN Boqiang,GAO Guang.Removal effects of periphyton on nitrogen and phosphorus in the eutrophic water body[J].Resources and Environment in the Yangtze Basin,2009,18(2):180-185.(in Chinese))
[16]SORENSEN J,JORGENSEN T,BRANDT S.Denitrification in stream epilithon:seasonal variation in gelbk and rabis baek denmark[J].Fems Microbiology Letters,1988,53(6):345-353.
[17]PALIJAN G,FUKS D,VIDAKOVIC J.Spatial and temporal distribution of net nitrite and nitrate production on submersed macrophyth ceratophyllum demersum[J].Fresenius Environmental Bulletin,2009,18(1):64-69.
[18]张来甲,叶春,李春华,等.沉水植物腐解对水体水质的影响[J].环境科学研究,2013,26(2):145-151.(ZHANG Laijia,YE Chun,LI Chunhua,et al.The effect of submerged macrophytes decomposition on water quality[J].Research of Environmental Sciences,2013,26(2):145-151.(in Chinese))
[19]VIS C,HUDON C,CARIGNAN R.Influence of the vertical structure of macrophyte stands on epiphyte community metabolism[J].Canadian Journal of Fisheries and Aquatic Sciences,2006,63(5):1014-1026.
[20]吕小央,张松贺,刘凯辉,等.水生植物-生物膜体系的生态功能与互作机制研究进展[J].水资源保护,2015,21(2):20-25.(LYU Xiaoyang,ZHANG Songhe,LIU Kaihui,et al.Advances in ecological function and interaction mechanism of aquatic macrophyte-biofilm system[J].Water Resources Protection,2015,21(2):20-25.(in Chinese))
[21]HIFNEY A F,FAWZY M A,ISSA A A,et al.Biodegradation of palisada perforata(rhodophyceae)and sargassum sp.(phaeophyceae)biomass by crude enzyme preparations from algicolous fungi[J].Journal of Applied Phycology,2015,27(6):2395-2404.
[22]ZWART G,CRUMP B C,AGTERVELD M,et al.Typical freshwater bacteria:an analysis of available 16S rRNA gene sequences from plankton of lakes and rivers[J].Aquatic Microbial Ecology,2002,28(2):141-155.
[23]杜瑞芳,李靖宇,赵吉.乌梁素海湖滨湿地细菌群落结构多样性[J].微生物学报,2014,54(10):1116-1128.(DU Ruifang,LI Jingyu,ZHAO Ji.Bacterial diversity in littoral wetland of Wuliangsuhai Lake[J].Acta Microbiologica Sinica,2014,54(10):1116-1128.(in Chinese))
[24]张雅洁,李珂,朱浩然,等.北海湖微生物群落结构随季节变化特征[J].环境科学,2017,38(8):3319-3329.(ZHANGYajie,LI Ke,ZHU Haoran,et al.Community structure of microorganisms and its seasonal variation in Beihai Lake[J].Environmental Science,2017,38(8):3319-3329.(in Chinese))
[25]金梦婷,朱亮,朱彧,等.城市缓流水体生物膜群落与环境因子响应关系[J].湖泊科学,2015,7(1):58-66.(JINMengying,ZHU Liang,ZHU Yu,et al.Relationship between the biofilm microbial communities and the environmental factors of municipal slow-flow water[J].Journal of Lake Sciences,2015,7(1):58-66.(in Chinese))
[26]朱亮,赵林多,刘钢,等.大运河沉积微生物群落结构特征分析[J].中国矿业大学学报,2010,39(2):295-301.(ZHULiang,ZHAO Linduo,LIU Gang,et al.Research on seasonal and spatial shifts in river sediments microbial community structure[J].Journal of China University of Mining and Technology,2010,39(2):295-301.(in Chinese))
[2]刘凯辉,张松贺,吕小央,等.南京花神湖3种沉水植物表面附着微生物群落特征[J].湖泊科学,2015,27(1):103-112.(LIU Kaihui,ZHANG Songhe,LYU Xiaoyang,et al.The characteristics of epiphytic microbes of three submerged macrophytes in Lake Huashen[J].Journal of Lake Sciences,2015,27(1):103-112.(in Chinese))
[3]HE D,REN L,WU Q.Epiphytic bacterial communities on two common submerged macrophytes in Taihu Lake:diversity and host-specificity[J].Chinese Journal of Oceanology and Limnology,2012,30(2):237-247.
[4]PANG S,ZHANG S,LYU X Y,et al.Characterization of bacterial community in biofilm and sediments of wetlands dominated by aquatic macrophytes[J].Ecological Engineering,2016,97:242-250.
[5]马杰.浅水湖泊典型沉水植物附着细菌群落结构特征[D].南京:南京师范大学,2016.
[6]POLLARD P C.Bacterial activity in plant(Schoenoplectus validus)biofilms of constructed wetlands[J].Water Research,2010,44(20):5939-5948.
[7]马杰,范婤,王国祥,等.洪泽湖3种沉水植物附着细菌群落结构及多样性[J].湖泊科学,2016,28(4):852-858.(MA Jie,FAN Zhou,WANG Guoxiang,et al.Diversity and community structure of epiphytic bacteria on different submerged macrophytes in Lake Hongze[J].Journal of Lake Sciences,2016,28(4):852-858.(in Chinese))
[8]由文辉.淀山湖着生藻类群落结构与数量特征[J].环境科学,1999,20(5):62-65.(YOU Wenhui.The species composition and quantitative features of the periphytic algae communities in Dianshan Lake[J].Environmental Science,1999,20(5):62-65.(in Chinese))
[9]常会庆,丁学峰,蔡景波.水生植物分泌物对微生物影响的研究[J].水土保持研究,2007,14(4):57-60.(CHANGHuiqing,DING Xuefeng,CAI Jingbo.Eeffects of crude exudates of a aquatic macrophytes on bacteria[J].Reasearch of Soil and Water Conservation,2007,14(4):57-60.(in Chinese))
[10]董彬,韩睿明,王国祥.沉水植物茎叶微界面特性研究进展[J].生态学报,2017,37(6):1769-1776.(DONG Bin,HANRuiming,WANG Guoxiang.Research advances in and perspectives on characteristics of the micro-boundary layer around leaves and stems of submerged macrophytes[J].Acta Ecologica Sinica,2017,37(6):1769-1776.(in Chinese))
[11]RAEDER U,RUZICKA J,GOOS C.Characterization of the light attenuation by periphyton in lakes of different trophic state[J].Limnologica,2010,40(1):40-46.
[12]杨飞,尹杰,张毅敏,等.两种沉水植物附着生物种群特征对水深的响应研究[J].环境科学学报,2018,38(6):1-14.(YANG Fei,YIN Jie,ZHANG Yimin,et al.Research on characteristics of epiphytic organism of two submerged macrophytes and its response to water depth[J].Acta Scientiae Circumstantiae,2018,38(6):1-14.(in Chinese))
[13]RUSCONI R,LECUYER S,AUTRUSSON N,et al.Secondary flow as a mechanism for the formation of biofilm streamers[J].Biophysical Journal,2011,100(12):3054.
[14]SANDJENSEN K,REVSBECH N P,RGENSEN B J.Microprofiles of oxygen in epiphyte communities on submerged macrophytes[J].Marine Biology,1985,89(1):55-62.
[15]宋玉芝,秦伯强,高光.附着生物对富营养化水体氮磷的去除效果[J].长江流域资源与环境,2009,18(2):180-185.(SONG Yuzhi,QIN Boqiang,GAO Guang.Removal effects of periphyton on nitrogen and phosphorus in the eutrophic water body[J].Resources and Environment in the Yangtze Basin,2009,18(2):180-185.(in Chinese))
[16]SORENSEN J,JORGENSEN T,BRANDT S.Denitrification in stream epilithon:seasonal variation in gelbk and rabis baek denmark[J].Fems Microbiology Letters,1988,53(6):345-353.
[17]PALIJAN G,FUKS D,VIDAKOVIC J.Spatial and temporal distribution of net nitrite and nitrate production on submersed macrophyth ceratophyllum demersum[J].Fresenius Environmental Bulletin,2009,18(1):64-69.
[18]张来甲,叶春,李春华,等.沉水植物腐解对水体水质的影响[J].环境科学研究,2013,26(2):145-151.(ZHANG Laijia,YE Chun,LI Chunhua,et al.The effect of submerged macrophytes decomposition on water quality[J].Research of Environmental Sciences,2013,26(2):145-151.(in Chinese))
[19]VIS C,HUDON C,CARIGNAN R.Influence of the vertical structure of macrophyte stands on epiphyte community metabolism[J].Canadian Journal of Fisheries and Aquatic Sciences,2006,63(5):1014-1026.
[20]吕小央,张松贺,刘凯辉,等.水生植物-生物膜体系的生态功能与互作机制研究进展[J].水资源保护,2015,21(2):20-25.(LYU Xiaoyang,ZHANG Songhe,LIU Kaihui,et al.Advances in ecological function and interaction mechanism of aquatic macrophyte-biofilm system[J].Water Resources Protection,2015,21(2):20-25.(in Chinese))
[21]HIFNEY A F,FAWZY M A,ISSA A A,et al.Biodegradation of palisada perforata(rhodophyceae)and sargassum sp.(phaeophyceae)biomass by crude enzyme preparations from algicolous fungi[J].Journal of Applied Phycology,2015,27(6):2395-2404.
[22]ZWART G,CRUMP B C,AGTERVELD M,et al.Typical freshwater bacteria:an analysis of available 16S rRNA gene sequences from plankton of lakes and rivers[J].Aquatic Microbial Ecology,2002,28(2):141-155.
[23]杜瑞芳,李靖宇,赵吉.乌梁素海湖滨湿地细菌群落结构多样性[J].微生物学报,2014,54(10):1116-1128.(DU Ruifang,LI Jingyu,ZHAO Ji.Bacterial diversity in littoral wetland of Wuliangsuhai Lake[J].Acta Microbiologica Sinica,2014,54(10):1116-1128.(in Chinese))
[24]张雅洁,李珂,朱浩然,等.北海湖微生物群落结构随季节变化特征[J].环境科学,2017,38(8):3319-3329.(ZHANGYajie,LI Ke,ZHU Haoran,et al.Community structure of microorganisms and its seasonal variation in Beihai Lake[J].Environmental Science,2017,38(8):3319-3329.(in Chinese))
[25]金梦婷,朱亮,朱彧,等.城市缓流水体生物膜群落与环境因子响应关系[J].湖泊科学,2015,7(1):58-66.(JINMengying,ZHU Liang,ZHU Yu,et al.Relationship between the biofilm microbial communities and the environmental factors of municipal slow-flow water[J].Journal of Lake Sciences,2015,7(1):58-66.(in Chinese))
[26]朱亮,赵林多,刘钢,等.大运河沉积微生物群落结构特征分析[J].中国矿业大学学报,2010,39(2):295-301.(ZHULiang,ZHAO Linduo,LIU Gang,et al.Research on seasonal and spatial shifts in river sediments microbial community structure[J].Journal of China University of Mining and Technology,2010,39(2):295-301.(in Chinese))
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |