掌叶覆盆子套种对板栗林土壤细菌群落结构演变及多样性的影响
|
摘要
通过研究掌叶覆盆子套种对栗林土壤细菌群落结构及多样性的影响,为科学评价该种植模式的生态风险提供科学依据。采集不同掌叶覆盆子套种密度(0、100和400株/亩)栗林土样,进行土壤主要化学指标及土壤细菌类群测定与分析。结果表明:不同模式间板栗林土壤细菌组成较一致,变形菌门(31. 20%)与酸杆菌门(29. 70%)为最多门类细菌。套种掌叶覆盆子后,土壤细菌Chao1指数显著提高,而Simpson指数显著提高,土壤细菌优势度增加,但多样性下降。套种和套种密度对土壤细菌群落的影响分别主要表现在门水平与目水平。板栗林套种掌叶覆盆子后,土壤有机质含量、全氮含量、水解性氮含量、有效磷含量、全磷含量显著增加。而土壤钾元素随套种密度提升呈现显著下降趋势。土壤理化性质的改变在一定程度上解释板栗-掌叶覆盆子模式中细菌群落结构变化,其中酸杆菌门、酸杆菌目与土壤氮含量变化相关性最大,绿弯菌门的大幅度减少可能同土壤中有机质增加有关。
In order to provide a scientific basis for evaluating the management measure of Castanea mollissima inter-planting with Rubus chingii,the impact of soil bacterial structure and biodiversity was studied. Soil samples from Castanea mollissima inter-planting with different density of Rubus chingii( 0,100 and 400 plants/Mu) were collected. Soil bacteria were sequenced by Illumina High Throughput Sequencing technique. And,the soil physical and chemical indexes were also analyzed. The results showed that soil bacterial structure of Castanea mollissima forest was similar among different modes, Proteobacteria( 31. 20%) and Acidobacteria( 29. 70%) were the most common bacteria. After inter-planting with Rubus chingii,Chao1 and Simpson index indicated dominance of soil bacteria were increased significantly,but diversity was decreased. The influences of inter-planting and density were mainly affected by soil bacterial community on the Phylum level and Order level,respectively. Castanea mollissima forest soil organic matter,total nitrogen,hydrolyzable nitrogen,available phosphorus and total phosphorus content were increased significantly by inter-planting with Rubus chingii. The soil potassium content was decreased with the increase of inter-planting density. The changes of soil physical and chemical property caused by inter-planting may explain the influence of bacterial community structure. The changes of Acidobacteria and Acidobacteriales were correlated with nitrogen content. The change of Chloroflexi was correlated with organic matter.
In order to provide a scientific basis for evaluating the management measure of Castanea mollissima inter-planting with Rubus chingii,the impact of soil bacterial structure and biodiversity was studied. Soil samples from Castanea mollissima inter-planting with different density of Rubus chingii( 0,100 and 400 plants/Mu) were collected. Soil bacteria were sequenced by Illumina High Throughput Sequencing technique. And,the soil physical and chemical indexes were also analyzed. The results showed that soil bacterial structure of Castanea mollissima forest was similar among different modes, Proteobacteria( 31. 20%) and Acidobacteria( 29. 70%) were the most common bacteria. After inter-planting with Rubus chingii,Chao1 and Simpson index indicated dominance of soil bacteria were increased significantly,but diversity was decreased. The influences of inter-planting and density were mainly affected by soil bacterial community on the Phylum level and Order level,respectively. Castanea mollissima forest soil organic matter,total nitrogen,hydrolyzable nitrogen,available phosphorus and total phosphorus content were increased significantly by inter-planting with Rubus chingii. The soil potassium content was decreased with the increase of inter-planting density. The changes of soil physical and chemical property caused by inter-planting may explain the influence of bacterial community structure. The changes of Acidobacteria and Acidobacteriales were correlated with nitrogen content. The change of Chloroflexi was correlated with organic matter.
引文
[1]马海泉,江锡兵,龚榜初,等.我国锥栗研究进展及发展对策[J].浙江林业科技,2013,33(1):62-67.
[2]陈云龙,叶浩然,徐永星,等.板栗低产林改造技术探讨及经济效益分析[J].经济林研究,2007,25(1):42-45.
[3]谢一辉,苗菊茹,刘文琴.覆盆子化学成分的研究[J].中药材,2005,28(2):99-100.
[4]郭启雷,杨峻山.掌叶覆盆子的化学成分研究[J].中国中药杂志,2007,42(15):198-200.
[5]孙健,沈晓霞.覆盆子的药用研究进展与鲜食产业分析[J].科技通报,2017,33(6):82-85.
[6]王光华,金剑,徐美娜,等.植物、土壤及土壤管理对土壤微生物群落结构的影响[J].生态学杂志,2006,25(5):550-556.
[7]KASCHUK G,ALBERTON O,HUNGRIA M. Quantifying effects of different agricultural land uses on soil microbial biomass and activity in Brazilian biomes:inferences to improve soil quality[J]. Plant&Soil,2011,338(1/2):467-481.
[8]宋彦君,季志平,吕平会,等.镇安板栗外生菌根真菌多样性研究[J].西北林学院学报,2016,31(4):188-194.
[9]秦岭,徐践,马萱,等.板栗共生菌根真菌种类及其发生规律的研究[J].北京农学院学报,1995(1):71-76.
[10]徐秋芳,姜培坤,邬奇峰,等.集约经营板栗林土壤微生物量碳与微生物多样性研究[J].林业科学,2007,43(3):15-19.
[11]张桂玲.秸秆腐熟物覆盖对板栗园土壤微生物、养分含量和产量的影响[J].果树学报,2012,29(6):1057-1062.
[12]ZHANG J,KOBERT K,FLOURI T,et al. PEAR:a fast and accurate Illumina Paired-End reAd mergeR[J]. Bioinformatics,2014,30(5):614.
[13]CHRISTINA J A,KEITH D,LAURA S W,et al. Sequencing ancient calcified dental plaque shows changes in oral microbiota with dietary shifts of the Neolithic and Industrial revolutions[J]. Nature Genetics,2013,45(4):450-455.
[14]邬枭楠.掌叶覆盆子山地种植技术[J].现代农业科技,2016(15):170-170.
[15]董敏,丁之恩,闫晗,等.板栗种实生长发育中营养物质积累及动态模型建立[J].核农学报,2016,30(6):1143-1148.
[16]周敏,毛曦,陈环,等.葡萄钾营养及其在果实中积累的研究进展[J].果树学报,2017,34(6):752-761.
[17]陈月星.覆盖对渭北旱作苹果园土壤微生物群落特征的影响[D].杨凌:西北农林科技大学,2015.
[18]BARNS S M,TAKALA S L,KUSKE C R. Wide distribution and diversity of members of the bacterial kingdom Acidobacterium in the environment.[J]. Applied&Environmental Microbiology,1999,65(4):1731-1737.
[19]翟婉璐,钟哲科,高贵宾,等.覆盖经营对雷竹林土壤细菌群落结构演变及多样性的影响[J].林业科学,2017,53(9):133-142.
[20]CAMPOS S B,LISBOA B B,CAMARGO F A O,et al. Soil suppressiveness and its relations with the microbial community in a Brazilian subtropical agroecosystem under different management systems[J]. Soil Biology&Biochemistry,2016,96:191-197.
[21]汪华.茶园土壤微生物群落结构对植茶年限、施肥和高温条件的响应研究[D].杭州:浙江大学,2014.
[22]ZHAO Y,WU L,CHU L,et al. Interaction of Pseudostellaria heterophylla with Fusarium oxysporum f. sp. heterophylla mediated by its root exudates in a consecutive monoculture system[J]. Scientific Reports,2015,5:81-97.
[23]FIERER N,BRADFORD M A,JACKSON R B. Toward an ecological classification of soil bacteria[J]. Ecology,2007,88(6):1354-1364.
[24]ZHANG Y,CONG J,LU H,et al. Community structure and elevational diversity patterns of soil Acidobacteria[J]. Jourmal of Environmental Sciences,2014,26(8):1717-1724.
[25]LIU J,SUI Y,YU Z,et al. Soil carbon content drives the biogeographical distribution of fungal communities in the black soil zone of northeast China[J]. Soil Biology&Biochemistry,2015,83:29-39.
[26]张俊华,张翼,李明.药用植物宁夏枸杞(Lycium barbarum L.)土壤细菌群落演替特征[J].植物营养与肥料学报,2017,23(3):686-695.
[27]刘文娜,吴文良,王秀斌,等.不同土壤类型和农业用地方式对土壤微生物量碳的影响[J].植物营养与肥料学报,2006,12(3):406-411.
[28]LATZ E,EISENHAUER N,RALL B C,et al. Plant diversity improves protection against soil-borne pathogens by fostering antagonistic bacterial communities[J]. Journal of Ecology,2012,100(3):597-604.
[29]MAGILL A H,ABER J D. Variation in soil net mineralization rates with dissolved organic carbon additions[J]. Soil Biology&Biochemistry,2000,32(5):597-601.
[30]李晨华,张彩霞,唐立松,等.长期施肥土壤微生物群落的剖面变化及其与土壤性质的关系[J].微生物学报,2014,54(3):319-329.
[2]陈云龙,叶浩然,徐永星,等.板栗低产林改造技术探讨及经济效益分析[J].经济林研究,2007,25(1):42-45.
[3]谢一辉,苗菊茹,刘文琴.覆盆子化学成分的研究[J].中药材,2005,28(2):99-100.
[4]郭启雷,杨峻山.掌叶覆盆子的化学成分研究[J].中国中药杂志,2007,42(15):198-200.
[5]孙健,沈晓霞.覆盆子的药用研究进展与鲜食产业分析[J].科技通报,2017,33(6):82-85.
[6]王光华,金剑,徐美娜,等.植物、土壤及土壤管理对土壤微生物群落结构的影响[J].生态学杂志,2006,25(5):550-556.
[7]KASCHUK G,ALBERTON O,HUNGRIA M. Quantifying effects of different agricultural land uses on soil microbial biomass and activity in Brazilian biomes:inferences to improve soil quality[J]. Plant&Soil,2011,338(1/2):467-481.
[8]宋彦君,季志平,吕平会,等.镇安板栗外生菌根真菌多样性研究[J].西北林学院学报,2016,31(4):188-194.
[9]秦岭,徐践,马萱,等.板栗共生菌根真菌种类及其发生规律的研究[J].北京农学院学报,1995(1):71-76.
[10]徐秋芳,姜培坤,邬奇峰,等.集约经营板栗林土壤微生物量碳与微生物多样性研究[J].林业科学,2007,43(3):15-19.
[11]张桂玲.秸秆腐熟物覆盖对板栗园土壤微生物、养分含量和产量的影响[J].果树学报,2012,29(6):1057-1062.
[12]ZHANG J,KOBERT K,FLOURI T,et al. PEAR:a fast and accurate Illumina Paired-End reAd mergeR[J]. Bioinformatics,2014,30(5):614.
[13]CHRISTINA J A,KEITH D,LAURA S W,et al. Sequencing ancient calcified dental plaque shows changes in oral microbiota with dietary shifts of the Neolithic and Industrial revolutions[J]. Nature Genetics,2013,45(4):450-455.
[14]邬枭楠.掌叶覆盆子山地种植技术[J].现代农业科技,2016(15):170-170.
[15]董敏,丁之恩,闫晗,等.板栗种实生长发育中营养物质积累及动态模型建立[J].核农学报,2016,30(6):1143-1148.
[16]周敏,毛曦,陈环,等.葡萄钾营养及其在果实中积累的研究进展[J].果树学报,2017,34(6):752-761.
[17]陈月星.覆盖对渭北旱作苹果园土壤微生物群落特征的影响[D].杨凌:西北农林科技大学,2015.
[18]BARNS S M,TAKALA S L,KUSKE C R. Wide distribution and diversity of members of the bacterial kingdom Acidobacterium in the environment.[J]. Applied&Environmental Microbiology,1999,65(4):1731-1737.
[19]翟婉璐,钟哲科,高贵宾,等.覆盖经营对雷竹林土壤细菌群落结构演变及多样性的影响[J].林业科学,2017,53(9):133-142.
[20]CAMPOS S B,LISBOA B B,CAMARGO F A O,et al. Soil suppressiveness and its relations with the microbial community in a Brazilian subtropical agroecosystem under different management systems[J]. Soil Biology&Biochemistry,2016,96:191-197.
[21]汪华.茶园土壤微生物群落结构对植茶年限、施肥和高温条件的响应研究[D].杭州:浙江大学,2014.
[22]ZHAO Y,WU L,CHU L,et al. Interaction of Pseudostellaria heterophylla with Fusarium oxysporum f. sp. heterophylla mediated by its root exudates in a consecutive monoculture system[J]. Scientific Reports,2015,5:81-97.
[23]FIERER N,BRADFORD M A,JACKSON R B. Toward an ecological classification of soil bacteria[J]. Ecology,2007,88(6):1354-1364.
[24]ZHANG Y,CONG J,LU H,et al. Community structure and elevational diversity patterns of soil Acidobacteria[J]. Jourmal of Environmental Sciences,2014,26(8):1717-1724.
[25]LIU J,SUI Y,YU Z,et al. Soil carbon content drives the biogeographical distribution of fungal communities in the black soil zone of northeast China[J]. Soil Biology&Biochemistry,2015,83:29-39.
[26]张俊华,张翼,李明.药用植物宁夏枸杞(Lycium barbarum L.)土壤细菌群落演替特征[J].植物营养与肥料学报,2017,23(3):686-695.
[27]刘文娜,吴文良,王秀斌,等.不同土壤类型和农业用地方式对土壤微生物量碳的影响[J].植物营养与肥料学报,2006,12(3):406-411.
[28]LATZ E,EISENHAUER N,RALL B C,et al. Plant diversity improves protection against soil-borne pathogens by fostering antagonistic bacterial communities[J]. Journal of Ecology,2012,100(3):597-604.
[29]MAGILL A H,ABER J D. Variation in soil net mineralization rates with dissolved organic carbon additions[J]. Soil Biology&Biochemistry,2000,32(5):597-601.
[30]李晨华,张彩霞,唐立松,等.长期施肥土壤微生物群落的剖面变化及其与土壤性质的关系[J].微生物学报,2014,54(3):319-329.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |