MBL2与MASPs家族蛋白质相互作用的生物信息学分析

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英文篇名：Bioinformatics analysis of interaction between MBL2 and MASPs family proteins 作者：程少文 ; 陈扬平 ; 张安强 ; 彭磊 ; 梁华平 ; 吕传柱 英文作者：CHENG Shao-wen;CHEN Yang-ping;ZHANG An-qiang;PENG Lei;LIANG Hua-ping;LYU Chuan-zhu;Trauma Center,First Affiliated Hospital of Hainan Medical University;Key Laboratory of Emergency and Trauma of Ministry of Education,Emergency and Trauma College,Hainan Medical University;State Key Laboratory of Trauma,Burns and Combined Injury,Institute of Surgery Research,Daping Hospital,Army Medical University;Emergency and Trauma College,Hainan Medical University; 关键词：甘露糖结合凝集素2 ; 丝氨酸蛋白酶 ; 生物信息学 英文关键词：Mannose-binding lectin 2;;Serine protease;;Bioinformatics 中文刊名：海南医学院学报 英文刊名：Journal of Hainan Medical University 机构：海南医学院第一附属医院创伤中心;急救与创伤研究教育部重点实验室;陆军军医大学大坪医院野战外科研究所创伤烧伤与复合伤国家重点实验室;海南医学院急诊创伤学院; 出版日期：2019-08-08 15:29 出版单位：海南医学院学报 年：2019 期：15 基金：国家自然科学基金(81860347);; 海南省自然科学基金(818MS140);; 海南省科协青年科技英才创新计划项目(QCXM201816);; 海南省卫生计生行业科研项目(18A200178));; 军事医学创新工程专项(18CXZ002)~~ 语种：中文; 页：6-9+14 页数：5 CN：46-1049/R ISSN：1007-1237 分类号：Q811.4;R392

摘要

目的:通过生物信息学方法对甘露糖结合凝集素2(MBL2)蛋白与甘露聚糖结合凝集素相关丝氨酸蛋白酶(MASPs)家族蛋白质的相互作用进行全面预测分析。方法:使用同源建模(Swiss-model)和折叠识别(Phyre2)分别来构建MBL2蛋白与MASPs家族蛋白质的结构,使用STRING数据库及ZDOCK3.02预测分析MBL2与MASP1、MASP2两个蛋白质的相互作用情况。结果:MBL2与MASP1和MASP2均存在直接相互作用,并且和COLEC11、COLEC10、FCN2、C4B等构成相互作用网络。MBL2与MASP1结合位点和MASP2的结合位点高度重叠。MBL2参与结合MASP1的残基,大部分(77%)也参与了与MASP2的结合,表明MBL2是通过同一个区域,分别与MASPs家族的蛋白质相互作用。结论:MBL2与MASPs家族蛋白质之间作用密切,从而可能在补体系统激活及机体免疫防御等方面发挥重要的作用。
        Objective: The interaction between mannose-binding lectin 2(MBL2) protein and mannose-binding lectin-related serine proteases(MASPs) family proteins was comprehensively predicted and analyzed by bioinformatics. Methods: Homology modeling(Swiss-model) and fold recognition(Phyre 2) were used to construct the structure of MBL2 protein and MASPs family proteins respectively. STRING database and ZDOCK 3.02 were used to predict and analyze the interaction between MBL2 and MASP1 and MASP2 proteins. Results: MBL2 had direct interaction with MASP1 and MASP2, and formed interaction network with COLEEC11, COLEC10, FCN2 and C4 B. The binding sites of MBL2 and MASP1 and MASP2 overlap highly. MBL2 participates in the binding of MASP1 residues, and most of them(77%) are also involved in the binding of MASP2, suggesting that MBL2 interacts with the proteins of MASPs family through the same region. Conclusion: MBL2 and MASPs family proteins play an important role in complement system activation and immune defense.

引文

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