紫花苜蓿对陇东黄土高原油污土壤场地生态修复的综合响应
|
摘要
为明确紫花苜蓿(Medicago sativa)在陇东黄土高原地区油污土壤场地生态修复过程中的响应情况,在长庆油田公司第二采油厂陇东油泥处理站进行为期3个月的实际场地修复试验,采用常规方法测定4种油污浓度对紫花苜蓿生长和生理指标的影响。结果显示,株高、根长、地下生物量、石油降解菌数量和初始总石油烃(TPH)降解率随油污浓度增加而逐步降低(P <0.05);油污浓度在7%时对其生物富集系数(BCF)抑制程度最大,但在1%和4%浓度时其生物转移系数(TF)变化不显著(P> 0.05);抗氧化酶体系随油污浓度增加呈先增后降的变化趋势,且在油污浓度为5%时达到最高。综上所述,采用紫花苜蓿在陇东黄土高原地区开展油污土壤场地修复时油污浓度不应超过5%。
In order to clarify the comprehensive response of Medicago sativa when used to ecologically remediate a site in eastern Gansu Province that contained oil-contaminated soil, a 3 month ecological site remediation program was implemented at the Sludge disposal station of a secondary oil production plant affiliated to the Petro china Changqing Oilfield Company. The growth responses and physiological indicators of Medicago sativa under stress at different total petroleum hydrocarbons(TPH) concentrations were determined. We found that plant height, root length, plant underground biomass, amount of TPH degrading bacteria, and the TPH degradation rate of Medicago sativa decreased as the TPH concentration increased(P < 0.05). The 7% TPH concentration significantly reduced the rhizosphere BCF index, but there were no significant differences between the 1% and 4% concentrations(P > 0.05). The activities of antioxidant enzymes showed a single hump-shaped tend, and the greatest antioxidant enzyme activity was observed when the TPH concentration was 5%. All the results indicated that the TPH concentration should not exceed 5% when Medicago sativa is used to ecologically remediate sites containing oil-contaminated soil in the eastern region of the Loess Plateau.
In order to clarify the comprehensive response of Medicago sativa when used to ecologically remediate a site in eastern Gansu Province that contained oil-contaminated soil, a 3 month ecological site remediation program was implemented at the Sludge disposal station of a secondary oil production plant affiliated to the Petro china Changqing Oilfield Company. The growth responses and physiological indicators of Medicago sativa under stress at different total petroleum hydrocarbons(TPH) concentrations were determined. We found that plant height, root length, plant underground biomass, amount of TPH degrading bacteria, and the TPH degradation rate of Medicago sativa decreased as the TPH concentration increased(P < 0.05). The 7% TPH concentration significantly reduced the rhizosphere BCF index, but there were no significant differences between the 1% and 4% concentrations(P > 0.05). The activities of antioxidant enzymes showed a single hump-shaped tend, and the greatest antioxidant enzyme activity was observed when the TPH concentration was 5%. All the results indicated that the TPH concentration should not exceed 5% when Medicago sativa is used to ecologically remediate sites containing oil-contaminated soil in the eastern region of the Loess Plateau.
引文
[1]王金成,井明博,段春燕,王国锋,石国玺,周天林.石油烃污染对陇东黄土高原土壤生物学及非生物学特性的影响.水土保持通报,2017,37(1):9-16.WANG J C,JING M B,DUAN C Y,WANG G F,SHI G X,ZHOU T L.Impact of crude oil pollution on soil biological and abiological properties in eastern Gansu Province.Bulletin of Soil and Water Conservation,2017,37(1):9-16.
[2]ALI E,NAYER A K S,MOHSEN O,MARYAM H,REZA G N.Remediation of saline soils contaminated with crude oil using the halophyte Salicornia persica in conjunction with hydrocarbon degrading bacteria.Journal of Environmental Management,2018,219:260-268.
[3]ROMAN M,LUKASZ C,AGNIESZKA P C,WOJCIECH J,ROZA B M.Rhizosphere as a tool to introduce a soil-isolated hydrocarbon-degrading bacterial consortium into a wetland environment.International Biodeterioration and Biodegradation,2015,97:135-142.
[4]黄建,田长彦,卞卫国,尹海龙,任静,陈春秀.4种盐生植物生长对土壤石油污染的响应.干旱区研究,2014,31(1):100-104.HUANG J,TIAN C Y,BIAN W G,YIN H L,REN J,CHEN C X.Response of growth of four halophyte species in oilcontaminated soil.Arid Zone Research,2014,31(1):100-104.
[5]张敏,梁红,高大文,张百慧,李欣平,郭晓虎.东北地区23种杂草对多环芳烃超积累特性研究.环境科学,2011,32(10):3088-3093.ZHANG M,LIANG H,GAO D W,ZHANG B H,LI X P,GUO X H.Characteristics of 23 species of weed in northeast of china hyperaccumulating pahs in contaminated soils.Environmental Science,2011,32(10):3088-3093.
[6]祁迎春,王建,王宏,任绵绵,山宝琴,同延安.陕北7种杂草种子对石油污染的耐受性研究.环境科技,2014,27(3):5-14.QI Y C,WANG J,WANG H,REN M M,SHAN B Q,TONG Y A.Responses of seed germination of 7 weed plants to petroleum contamination.Environmental Science and Technology,2014,27(3):5-14.
[7]JANMEJAY P,ARCHANA C,RAKESH K J.Integrative approaches for assessing the ecological sustainability of in situ bioremediation.Microbiology Review,2013,33:324-375.
[8]王金成,井明博,段春艳,张希彪,刘建新,肖朝霞,周天林.陇东黄土高原石油污染土壤环境因子对金盏菊(Calendula officinalis)-微生物联合修复的响应.环境科学学报,2015,35(9):2971-2981.WANG J C,JING M B,DUAN C Y,ZHANG X B,LIU J X,XIAO Z X,ZHOU T L.Environmental factors responses to petroleum-contaminated soil in situ remediation by using a combination of Calendula officinalis and microbial agent in eastern Gansu Province.Acta Scientiae Circumstantiae,2015,35(9):2971-2981.
[9]王传远,杨翠云,孙志高,杨玉玮,瞿成利,王允周.黄河三角洲生态区土壤石油污染及其与理化性质的关系.水土保持学报,2010,24(2):214-217.WANG C H,YANG C Y,SUN Z G,YANG Y W,QU C L,WANG Y Z.Contamination characteristics and its relationship with physico-chemical properties of oil polluted soils in the yellow river delta swamp.Journal of Soil and Water Conservation,2010,24(2):214-217.
[10]王金成,井明博,陈拓,杨蕊琪,王国锋,周天林.陇东黄土高原油污土壤环境因子对场地生态修复的季节响应.水土保持通报,2017,37(5):1-9.WANG J C,JING M B,CHEN T,YANG R Q,WANG G F,ZHOU T L.Season response and soil environmental factors to in-situ ecological remediation of crude-oil contaminated soil in eastern Gansu Province of loess plateau.Bulletin of Soil and Water Conservation,2017,37(5):1-9.
[11]李欢,张俊伶,王冲,向丹.丛枝菌根真菌对紫花苜蓿凋落物降解的研究.草业科学,2009,26(7):40-43.LI H,ZHANG J L,WANG C,XIANG D.Study of arbuscular mycorrhzizal fungi on decomposition of alfalfa litter.Pratacultural Science,2009,26(7):40-43.
[12]岳冰冰,李鑫,任芳菲,孟凡娟,孙广玉.石油污染对紫花苜蓿部分生理指标的影响.草业科学,2011,28(2):236-240.YUE B B,LI X,REN F F,MENG F J,SUN G Y.Effects of petroleum contamination on some of physiological indexes of alfalfa.Pratacultural Science,2011,28(2):236-240.
[13]张丽妍,杨恒山,张宏宇,葛选良,谷永丽.灌溉条件下不同生长年限紫花苜蓿生长及生产能力的研究.草业科学,2010,27(1):85-91.ZHANG L Y,YANG H S,ZHANG H Y,GE X L,GU Y L.Study on the growth and productivity of alfalfa with different growth years under irrigation condition.Pratacultural Science,2010,27(1):85-91.
[14]程立娟,周启星.野生观赏植物长药八宝对石油烃污染土壤的修复研究.环境科学学报,2014,34(4):980-986.CHENG L J,ZHOU Q X.Phytoremediation of petroleum hydrocarbon contaminated soils using a wild ornamental plant Hylotelephium spectabile(Boreau)H.Ohba.Acta Scientiae Circumstantiae,2014,34(4):980-986.
[15]张怀山,赵桂琴,栗孟飞,夏曾润,王春梅.中型狼尾草幼苗对PEG、低温和盐胁迫的生理应答.草业学报,2014,23(2):180-188.ZHANG H S,ZHAO G Q,LI M F,XIA Z R,WANG C M.Physiological responses of Pennisetum longissimum var.intermedium seedlings to PEG low temperature and salt stress treatments.Acta Pratculturae Sinica,2014,23(2):180-188.
[16]赵世杰,许长城,邹琦,孟庆伟.植物组织中丙二醛测定方法的改进.植物生理学通讯,1991,30(3):207-210.ZHAO S J,XU C C,ZOU Q,MENG Q W.Improvements of method for measurement of Malondialdehvde in plant tissues.Plant Physiology Communications,1991,30(3):207-210.
[17]DHINDSA R,MATOWE W.Drought tolerance in two mosses:Correlated with enzymatic defence Against Lipid Peroxidation.Journal of Experimental Botany,1981,33:32(1):79-91.
[18]RAO M V,PALIYATH G,ORMROD D P.Ultraviolet-B-and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana.Plant Physiology,1996,110(1):125-136.
[19]ALI M B,VAJPAYEE P,TRIPATHI R D,RAI U N,SINGH S N,SINGH S P.Phytoremediation of Lead,Nickel,and Copper by Salix acmophylla Boiss.:Role of antioxidant enzymes and antioxidant substances.Bulletin of Environmental Contamination and Toxicology,2003,70:462-469.
[20]王如刚,王敏,牛晓伟,唐景春.超声-索氏萃取-重量法测定土壤中总石油烃含量.分析化学,2010,38(3):417-420.WANG R G,WANG M,NIU X W,TANG J C.Determination of total petroleum hydrocarbons content in soil by Ultrasonic-Soxhlet Extraction-Gravimetric analysis.Chinese Journal of Analytical Chemistry,2010,38(3):417-420.
[21]TANG T C,WANG R G,NIU X W,ZHOU Q X.Enhancement of soil petroleum remediation by using a combination of ryegrass(Lolium perenne)and different microorganisms.Soil&Tillage Research,2009,110:87-93.
[22]李先梅,肖易,吴芸紫,韦政伟,金涛,周建利,田应兵.华北油田石油污染土壤的修复植物筛选.环境科学与技术,2015,38(6):14-19.LI X M,XIAO Y,WU Y Z,WEI Z W,JIN T,ZHOU J L,TIAN Y B.Screening of remediation plant for petroleum contaminated soils in Huabei Oilfield.Environmental Science&Technology,2015,38(6):14-19.
[23]张麟君,李凯荣,张晓阳.陕北黄土高原不同植物对石油污染物的吸收与积累.西北农林科技大学学报(自然科学版),2013,41(8):110-116.ZHANG L J,LI K R,ZHANG X Y.Absorption and accumulation of petroleum contaminant by different plants in the Loess Plateau.Journal of Northwest A&F University(Nature and Science Edition),2013,41(8):110-116.
[24]山宝琴,张永涛,曹巧玲,康振妍,李淑媛.6种陕北适生豆科植物生长对原油污染土壤的响应.环境科学,2014,35(4):1125-1130.SHAN B Q,ZHANG Y T,CAO Q L,KANG Z Y,LI S Y.Growth responses of six Leguminous plants adaptable in northern Shaanxi to petroleum contaminated soil.Environmental Science,2014,35(4):1125-1130.
[25]时腾飞,刘增文,田楠,李俊,陈昕,王国保.黄土区石油污染对土壤及豆科灌草植物的潜在影响.草地学报,2013,21(2):295-301.SHI T F,LIU Z W,TIAN N,LI J,CHEN X,WANG B G.Potential influences of petroleum pollution on soil and Legume Shrubs and grasses in the Loess area.Acta Agrectir Sinica,2013,21(2):295-301.
[26]雒晓芳,陈丽华,王冬梅,陈芳艳.不同石油浓度对两种作物生理生化的影响.生物技术通报,2016,32(10):135-140.LUO X F,CHEN L H,WANG D M,CHEN F Y.The influence for two corps physiological index by the different petroleum density.Biotechnology Bulletin,2016,32(10):135-140.
[27]沈伟航,朱能武,王华金,党志,陈小强.石油污染土壤生物修复过程中毒性的植物指示.农业环境科学学报,2015,34(1):22-28.SHEN W H,ZHU N W,WANG H J,DANG Z,CHEN X Q.Plant bio-indicators of eco-toxicity of oil-contaminated soil during bioremediation.Journal of Agro-Environment Science,2015,34(1):22-28.
[28]JEAN P S.Phytoremediation of soils contaminated by organic compounds:Hype,hope and facts.Journal of Soils and Sediments,2017,17:1492-1502.
[29]于君宝,阚兴艳,王雪宏,韩广轩,管博,谢文军,林乾新.黄河三角洲石油污染对湿地芦苇和碱蓬幼苗生长影响的模拟研究.地理科学,2012,32(10):1254-1260.YU J B,KAN X Y,WANG X H,HAN G X,GUAN B,XIE W J,LIN Q X.Simulative Study on effects of petroleum contamination on seedling growth of Phragmites australis and Suaeda salsa in coastal wetland of Yellow river delta.Scientia Geographica Sinica,2012,32(10):1254-1260.
[30]弓晓峰,荣亮,杨丽珍,刘足根,方红亚.石油污染土壤对玉米生长的影响及其生态毒性研究.环境科学与技术,2011,34(10):71-75.GONG X F,RONG L,YANG L Z,LIU Z G,FANG H Y.Effect and eco-toxicity of maize growth in petroleum contaminated soil.Environmental Science&Technology,2011,34(10):71-75.
[31]崔碧霄,韩刚,李凯荣,王波.土壤石油污染下柠条生长反应与抗氧化保护响应.草业科学,2014,31(4):632-640.CUI B X,HAN G,LI K R,WANG B.Response of growth and antioxidant protection system of Caragana korshins oilcontaminated soil.Pratacultural Science,2014,31(4):632-640.
[32]宋雪英,宋玉芳,孙铁珩,李昕馨,张薇,周启星.石油污染土壤植物修复后对陆生高等植物的生态毒性.环境科学,2006,27(9):1866-1871.SONG X Y,SONG Y F,SUN T H,LI X X,ZHANG W,ZHOU Q X.Soil-based eco-toxicity of petroleum to terrestrial higher plant after phytoremediation.Environmental Science,2006,27(9):1866-1871.
[33]CHANG S J,ZHANG G S,CHEN X M,LONG H Z,WANG Y L,CHEN T,LIU G X.The complete genome sequence of the cold adapted crude-oil degrader:Pedobacter steynii DX4.Standards in Genomic Sciences,2017,45(12):1-8.
[2]ALI E,NAYER A K S,MOHSEN O,MARYAM H,REZA G N.Remediation of saline soils contaminated with crude oil using the halophyte Salicornia persica in conjunction with hydrocarbon degrading bacteria.Journal of Environmental Management,2018,219:260-268.
[3]ROMAN M,LUKASZ C,AGNIESZKA P C,WOJCIECH J,ROZA B M.Rhizosphere as a tool to introduce a soil-isolated hydrocarbon-degrading bacterial consortium into a wetland environment.International Biodeterioration and Biodegradation,2015,97:135-142.
[4]黄建,田长彦,卞卫国,尹海龙,任静,陈春秀.4种盐生植物生长对土壤石油污染的响应.干旱区研究,2014,31(1):100-104.HUANG J,TIAN C Y,BIAN W G,YIN H L,REN J,CHEN C X.Response of growth of four halophyte species in oilcontaminated soil.Arid Zone Research,2014,31(1):100-104.
[5]张敏,梁红,高大文,张百慧,李欣平,郭晓虎.东北地区23种杂草对多环芳烃超积累特性研究.环境科学,2011,32(10):3088-3093.ZHANG M,LIANG H,GAO D W,ZHANG B H,LI X P,GUO X H.Characteristics of 23 species of weed in northeast of china hyperaccumulating pahs in contaminated soils.Environmental Science,2011,32(10):3088-3093.
[6]祁迎春,王建,王宏,任绵绵,山宝琴,同延安.陕北7种杂草种子对石油污染的耐受性研究.环境科技,2014,27(3):5-14.QI Y C,WANG J,WANG H,REN M M,SHAN B Q,TONG Y A.Responses of seed germination of 7 weed plants to petroleum contamination.Environmental Science and Technology,2014,27(3):5-14.
[7]JANMEJAY P,ARCHANA C,RAKESH K J.Integrative approaches for assessing the ecological sustainability of in situ bioremediation.Microbiology Review,2013,33:324-375.
[8]王金成,井明博,段春艳,张希彪,刘建新,肖朝霞,周天林.陇东黄土高原石油污染土壤环境因子对金盏菊(Calendula officinalis)-微生物联合修复的响应.环境科学学报,2015,35(9):2971-2981.WANG J C,JING M B,DUAN C Y,ZHANG X B,LIU J X,XIAO Z X,ZHOU T L.Environmental factors responses to petroleum-contaminated soil in situ remediation by using a combination of Calendula officinalis and microbial agent in eastern Gansu Province.Acta Scientiae Circumstantiae,2015,35(9):2971-2981.
[9]王传远,杨翠云,孙志高,杨玉玮,瞿成利,王允周.黄河三角洲生态区土壤石油污染及其与理化性质的关系.水土保持学报,2010,24(2):214-217.WANG C H,YANG C Y,SUN Z G,YANG Y W,QU C L,WANG Y Z.Contamination characteristics and its relationship with physico-chemical properties of oil polluted soils in the yellow river delta swamp.Journal of Soil and Water Conservation,2010,24(2):214-217.
[10]王金成,井明博,陈拓,杨蕊琪,王国锋,周天林.陇东黄土高原油污土壤环境因子对场地生态修复的季节响应.水土保持通报,2017,37(5):1-9.WANG J C,JING M B,CHEN T,YANG R Q,WANG G F,ZHOU T L.Season response and soil environmental factors to in-situ ecological remediation of crude-oil contaminated soil in eastern Gansu Province of loess plateau.Bulletin of Soil and Water Conservation,2017,37(5):1-9.
[11]李欢,张俊伶,王冲,向丹.丛枝菌根真菌对紫花苜蓿凋落物降解的研究.草业科学,2009,26(7):40-43.LI H,ZHANG J L,WANG C,XIANG D.Study of arbuscular mycorrhzizal fungi on decomposition of alfalfa litter.Pratacultural Science,2009,26(7):40-43.
[12]岳冰冰,李鑫,任芳菲,孟凡娟,孙广玉.石油污染对紫花苜蓿部分生理指标的影响.草业科学,2011,28(2):236-240.YUE B B,LI X,REN F F,MENG F J,SUN G Y.Effects of petroleum contamination on some of physiological indexes of alfalfa.Pratacultural Science,2011,28(2):236-240.
[13]张丽妍,杨恒山,张宏宇,葛选良,谷永丽.灌溉条件下不同生长年限紫花苜蓿生长及生产能力的研究.草业科学,2010,27(1):85-91.ZHANG L Y,YANG H S,ZHANG H Y,GE X L,GU Y L.Study on the growth and productivity of alfalfa with different growth years under irrigation condition.Pratacultural Science,2010,27(1):85-91.
[14]程立娟,周启星.野生观赏植物长药八宝对石油烃污染土壤的修复研究.环境科学学报,2014,34(4):980-986.CHENG L J,ZHOU Q X.Phytoremediation of petroleum hydrocarbon contaminated soils using a wild ornamental plant Hylotelephium spectabile(Boreau)H.Ohba.Acta Scientiae Circumstantiae,2014,34(4):980-986.
[15]张怀山,赵桂琴,栗孟飞,夏曾润,王春梅.中型狼尾草幼苗对PEG、低温和盐胁迫的生理应答.草业学报,2014,23(2):180-188.ZHANG H S,ZHAO G Q,LI M F,XIA Z R,WANG C M.Physiological responses of Pennisetum longissimum var.intermedium seedlings to PEG low temperature and salt stress treatments.Acta Pratculturae Sinica,2014,23(2):180-188.
[16]赵世杰,许长城,邹琦,孟庆伟.植物组织中丙二醛测定方法的改进.植物生理学通讯,1991,30(3):207-210.ZHAO S J,XU C C,ZOU Q,MENG Q W.Improvements of method for measurement of Malondialdehvde in plant tissues.Plant Physiology Communications,1991,30(3):207-210.
[17]DHINDSA R,MATOWE W.Drought tolerance in two mosses:Correlated with enzymatic defence Against Lipid Peroxidation.Journal of Experimental Botany,1981,33:32(1):79-91.
[18]RAO M V,PALIYATH G,ORMROD D P.Ultraviolet-B-and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana.Plant Physiology,1996,110(1):125-136.
[19]ALI M B,VAJPAYEE P,TRIPATHI R D,RAI U N,SINGH S N,SINGH S P.Phytoremediation of Lead,Nickel,and Copper by Salix acmophylla Boiss.:Role of antioxidant enzymes and antioxidant substances.Bulletin of Environmental Contamination and Toxicology,2003,70:462-469.
[20]王如刚,王敏,牛晓伟,唐景春.超声-索氏萃取-重量法测定土壤中总石油烃含量.分析化学,2010,38(3):417-420.WANG R G,WANG M,NIU X W,TANG J C.Determination of total petroleum hydrocarbons content in soil by Ultrasonic-Soxhlet Extraction-Gravimetric analysis.Chinese Journal of Analytical Chemistry,2010,38(3):417-420.
[21]TANG T C,WANG R G,NIU X W,ZHOU Q X.Enhancement of soil petroleum remediation by using a combination of ryegrass(Lolium perenne)and different microorganisms.Soil&Tillage Research,2009,110:87-93.
[22]李先梅,肖易,吴芸紫,韦政伟,金涛,周建利,田应兵.华北油田石油污染土壤的修复植物筛选.环境科学与技术,2015,38(6):14-19.LI X M,XIAO Y,WU Y Z,WEI Z W,JIN T,ZHOU J L,TIAN Y B.Screening of remediation plant for petroleum contaminated soils in Huabei Oilfield.Environmental Science&Technology,2015,38(6):14-19.
[23]张麟君,李凯荣,张晓阳.陕北黄土高原不同植物对石油污染物的吸收与积累.西北农林科技大学学报(自然科学版),2013,41(8):110-116.ZHANG L J,LI K R,ZHANG X Y.Absorption and accumulation of petroleum contaminant by different plants in the Loess Plateau.Journal of Northwest A&F University(Nature and Science Edition),2013,41(8):110-116.
[24]山宝琴,张永涛,曹巧玲,康振妍,李淑媛.6种陕北适生豆科植物生长对原油污染土壤的响应.环境科学,2014,35(4):1125-1130.SHAN B Q,ZHANG Y T,CAO Q L,KANG Z Y,LI S Y.Growth responses of six Leguminous plants adaptable in northern Shaanxi to petroleum contaminated soil.Environmental Science,2014,35(4):1125-1130.
[25]时腾飞,刘增文,田楠,李俊,陈昕,王国保.黄土区石油污染对土壤及豆科灌草植物的潜在影响.草地学报,2013,21(2):295-301.SHI T F,LIU Z W,TIAN N,LI J,CHEN X,WANG B G.Potential influences of petroleum pollution on soil and Legume Shrubs and grasses in the Loess area.Acta Agrectir Sinica,2013,21(2):295-301.
[26]雒晓芳,陈丽华,王冬梅,陈芳艳.不同石油浓度对两种作物生理生化的影响.生物技术通报,2016,32(10):135-140.LUO X F,CHEN L H,WANG D M,CHEN F Y.The influence for two corps physiological index by the different petroleum density.Biotechnology Bulletin,2016,32(10):135-140.
[27]沈伟航,朱能武,王华金,党志,陈小强.石油污染土壤生物修复过程中毒性的植物指示.农业环境科学学报,2015,34(1):22-28.SHEN W H,ZHU N W,WANG H J,DANG Z,CHEN X Q.Plant bio-indicators of eco-toxicity of oil-contaminated soil during bioremediation.Journal of Agro-Environment Science,2015,34(1):22-28.
[28]JEAN P S.Phytoremediation of soils contaminated by organic compounds:Hype,hope and facts.Journal of Soils and Sediments,2017,17:1492-1502.
[29]于君宝,阚兴艳,王雪宏,韩广轩,管博,谢文军,林乾新.黄河三角洲石油污染对湿地芦苇和碱蓬幼苗生长影响的模拟研究.地理科学,2012,32(10):1254-1260.YU J B,KAN X Y,WANG X H,HAN G X,GUAN B,XIE W J,LIN Q X.Simulative Study on effects of petroleum contamination on seedling growth of Phragmites australis and Suaeda salsa in coastal wetland of Yellow river delta.Scientia Geographica Sinica,2012,32(10):1254-1260.
[30]弓晓峰,荣亮,杨丽珍,刘足根,方红亚.石油污染土壤对玉米生长的影响及其生态毒性研究.环境科学与技术,2011,34(10):71-75.GONG X F,RONG L,YANG L Z,LIU Z G,FANG H Y.Effect and eco-toxicity of maize growth in petroleum contaminated soil.Environmental Science&Technology,2011,34(10):71-75.
[31]崔碧霄,韩刚,李凯荣,王波.土壤石油污染下柠条生长反应与抗氧化保护响应.草业科学,2014,31(4):632-640.CUI B X,HAN G,LI K R,WANG B.Response of growth and antioxidant protection system of Caragana korshins oilcontaminated soil.Pratacultural Science,2014,31(4):632-640.
[32]宋雪英,宋玉芳,孙铁珩,李昕馨,张薇,周启星.石油污染土壤植物修复后对陆生高等植物的生态毒性.环境科学,2006,27(9):1866-1871.SONG X Y,SONG Y F,SUN T H,LI X X,ZHANG W,ZHOU Q X.Soil-based eco-toxicity of petroleum to terrestrial higher plant after phytoremediation.Environmental Science,2006,27(9):1866-1871.
[33]CHANG S J,ZHANG G S,CHEN X M,LONG H Z,WANG Y L,CHEN T,LIU G X.The complete genome sequence of the cold adapted crude-oil degrader:Pedobacter steynii DX4.Standards in Genomic Sciences,2017,45(12):1-8.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |