鲁中肉羊BMPR1B、BMP15和GDF9基因多态性与产羔数关联分析
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摘要
为分析绵羊已知多羔主效基因BMPR1B、BMP15和GDF9的多态性与鲁中肉羊产羔数之间的关系,采用Sequenom MassARRAY誖SNP技术检测鲁中肉羊BMPR1B、BMP15和GDF9基因中已知主效位点多态性,并与产羔数进行关联分析。结果表明:BMPR1B基因在鲁中肉羊中存在FecB突变,GDF9基因6个分型位点仅4个位点(G1、G3、G4、G5)在鲁中肉羊中存在多态,BMP15基因中5个分型位点在鲁中肉羊中均不存在多态性。FecB基因3种基因型(CC、CT、TT)突变频率分别为0.16、0.58和0.26,此位点多态性较高(0.25多态性较高(0.25 To elucidate the association of polymorphisms of BMPR1 B,BMP15 and GDF9 genes and litter size in sheep. Luzhong mutton sheep were selected and Sequenom Mass ARRAY ?SNP assay was applied to genotype BMPR1 B,BMP15 and GDF9 genes.Then the association was analyzed between BMPR1 B,BMP15 and GDF9 genes and litter size in Luzhong mutton sheep. The typing results showed that there were FecB mutation in BMPR1 B gene and 4 mutations(G1,G3,G4,G5)in GDF9 gene. There were no polymorphisms of BMP15 in our population. FecB had CC,CT and TT genotypes,and the genotype frequencies were 0.16,0.58 and0.26,respectively. This locus showed medium polymorphism(0.25
引文
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[20]田志龙,刘秋月,王翔宇,等.绵羊BMP15基因多态性及其与产羔数的关联分析[J].中国畜牧杂志,2018,54(7):23-27.
[21]《国家畜禽遗传资源委员会》编写组.中国畜禽遗传资源志·羊志[M].北京:中国农业出版社,2011.
[22]张坤.宁夏4个绵羊群体繁殖相关基因SNP的检测与分析[D].银川:宁夏大学,2018.
[23]董新龙,胡文萍,贺小云,等.绵羊BMP15组织表达特征及FecX~(Gr)、FecX~O和G971A突变的检测[J].农业生物技术学报,2016,24(12):1810-1819.
[24]狄冉,冯涛,储明星,等.绵羊和山羊BMP15基因FecXR突变的检测[J].畜牧兽医学报,2009,40(9):1303-1307.
[25]Tang J,Hu W,Di R,et al.Expression Analysis of the Prolific Candidate Genes,BMPR1B,BMP15,and GDF9 in Small Tail Han ewes with Three Fecundity(FecB Gene)Genotypes[J].Animals(Basel),2018,8(10):E166.
[26]金慧慧,储明星,潘章源,等.16种山羊和6种绵羊GDF9基因G7和Fec G^V突变检测[J].安徽农业大学学报,2015,42(1):104-109.
[27]左北瑶,钱宏光,刘佳森,等.德国肉用美利奴羊BMPR-IB、BMP15和GDF9基因10个突变位点的多态性检测分析[J].南京农业大学学报,2012,35(3):114-120.
[28]雷梦媛,潘章源,狄冉,等.山羊和绵羊GDF9基因Fec G~E突变检测[J].扬州大学学报(农业与生命科学版),2015(1):38-41.
[29]潘章源,贺小云,刘秋月,等.绵羊GDF9基因m RNA、DNA和调控区序列克隆及其在11个品种中遗传多态性检测[J].畜牧兽医学报,2016,47(8):1555-1564.
[2] Davis G H,Montgomery G W,Allison A J,et al.Segregation of a major gene influencing fecundity in progeny of Booroola sheep[J].New Zeal J Agr Res,1982,25(4):525-529.
[3] Montgomery G,Crawford A,Penty J,et al.The ovine Booroola fecundity gene(FecB)is linked to markers from a region of human chromosome 4q[J].Nature Genetics,1993,4(4):410-414.
[4] Christoforou E R,Pitman J L.Intra-follicular growth differentiation factor 9:Bone morphogenetic 15 ratio determines litter size in mammals[J].Biology of Reproduction,2019.DOI:10.1093/biolre/ioz011.
[5] Zamani P,Nadri S,Saffaripour R,et al.A new mutation in exon 2 of the bone morphogenetic protein 15 gene is associated with increase in prolificacy of Mehraban and Lori sheep[J].Trop Anim Health Prod,2015,47(5):855-860.
[6] Bodin L,Di Pasquale E,Fabre S,et al.A novel mutation in the bone morphogenetic protein 15 gene causing defective protein secretion is associated with both increased ovulation rate and sterility in Lacaune sheep[J].Endocrinology,2007,148(1):393-400.
[7] Galloway S M,Mcnatty K P,Cambridge L M,et al.Mutations in an oocyte-derived growth factor gene(BMP15)cause increased ovulation rate and infertility in a dosage-sensitive manner[J].Nat Genet,2000,25(3):279-283.
[8] Martinez-Royo A,Jurado J J,Smulders J P,et al.A deletion in the bone morphogenetic protein 15 gene causes sterility and increased prolificacy in Rasa Aragonesa sheep[J].Animal Genetics,2008,39(3):294-297.
[9] Monteagudo L V,Ponz R,Tejedor M T,et al.A 17 bp deletion in the bone morphogenetic protein 15(BMP15)gene is associated to increased prolificacy in the Rasa Aragonesa sheep breed[J].Animal Reproduction Science,2009,110(1/2):139-146.
[10] Liu Q,Pan Z,Wang X,et al.Progress on major genes for high fecundity in ewes[J].Frontier Journal of Agricultural Science and Engineering,2014,1(4):282-290.
[11]Ortega M S,Denicol A C,Cole J B,et al.Use of single nucleotide polymorphisms in candidate genes associated with daughter pregnancy rate for prediction of genetic merit for reproduction in Holstein cows[J].Animal Genetics,2016,47(3):288-297.
[12]周梅,曹晓涵,贺小云,等.绵羊FGF 7基因组织表达及其多态性与产羔数之间的关系[J].畜牧兽医学报,2018,49(3):525-533.
[13]Mulsant P,Lecerf F,Fabre S,et al.Mutation in bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booroola Merino ewes[J].Proceedings of the National Academy of Sciences of the United States of America,2001,98(9):5104-5109.
[14] Souza C J H,Macdougall C,Campbell B K,et al.The Booroola(FecB)phenotype is associated with a mutation in the bone morphogenetic receptor type 1B(BMPR1B)gene[J].Journal of Endocrinology,2001,169(2):R1-R6.
[15]Wilson T,Wu X Y,Juengel J L,et al.Highly prolific Booroola sheep have a mutation in the intracellular kinase domain of bone morphogenetic protein IB receptor(ALK-6)that is expressed in both oocytes and granulosa cells[J].Biology of Reproduction,2001,64(4):1225-1235.
[16]Davis G,Bruce G,Dodds K.Ovulation rate and litter size of prolific Inverdale(FecXI)and Hanna(FecXH)sheep[J].Proceeding of Association for the Advancement of Animal Breeding and Genetics,2001,14:175-178.
[17]Hanrahan J P,Gregan S M,Mulsant P,et al.Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep(Ovis aries)[J].Biology of Reproduction,2004,70(4):900-909.
[18]Shackell G,Hudson N,Heath D,et al.Plasma gonadotropin concentrations and ovarian characteristics in Inverdale ewes that are heterozygous for a major gene(FecXI)on the X chromosome that influences ovulation rate[J].Biology of Reproduction,1993,48(5):1150-1156.
[19]Lassoued N,Benkhlil Z,Woloszyn F,et al.FecX Bar a Novel BMP15mutation responsible for prolificacy and female sterility in Tunisian Barbarine sheep[J].Bmc Genetics,2017,18(1):43-52.
[20]田志龙,刘秋月,王翔宇,等.绵羊BMP15基因多态性及其与产羔数的关联分析[J].中国畜牧杂志,2018,54(7):23-27.
[21]《国家畜禽遗传资源委员会》编写组.中国畜禽遗传资源志·羊志[M].北京:中国农业出版社,2011.
[22]张坤.宁夏4个绵羊群体繁殖相关基因SNP的检测与分析[D].银川:宁夏大学,2018.
[23]董新龙,胡文萍,贺小云,等.绵羊BMP15组织表达特征及FecX~(Gr)、FecX~O和G971A突变的检测[J].农业生物技术学报,2016,24(12):1810-1819.
[24]狄冉,冯涛,储明星,等.绵羊和山羊BMP15基因FecXR突变的检测[J].畜牧兽医学报,2009,40(9):1303-1307.
[25]Tang J,Hu W,Di R,et al.Expression Analysis of the Prolific Candidate Genes,BMPR1B,BMP15,and GDF9 in Small Tail Han ewes with Three Fecundity(FecB Gene)Genotypes[J].Animals(Basel),2018,8(10):E166.
[26]金慧慧,储明星,潘章源,等.16种山羊和6种绵羊GDF9基因G7和Fec G^V突变检测[J].安徽农业大学学报,2015,42(1):104-109.
[27]左北瑶,钱宏光,刘佳森,等.德国肉用美利奴羊BMPR-IB、BMP15和GDF9基因10个突变位点的多态性检测分析[J].南京农业大学学报,2012,35(3):114-120.
[28]雷梦媛,潘章源,狄冉,等.山羊和绵羊GDF9基因Fec G~E突变检测[J].扬州大学学报(农业与生命科学版),2015(1):38-41.
[29]潘章源,贺小云,刘秋月,等.绵羊GDF9基因m RNA、DNA和调控区序列克隆及其在11个品种中遗传多态性检测[J].畜牧兽医学报,2016,47(8):1555-1564.
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