景天三七主要活性成分与土壤、气象因子的相关性
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摘要
以3个引种区12个试验点的景天三七(Sedumaizoon)的茎叶为研究对象,探究景天三七活性成分与生态因子间的相关性,通过冗余分析(RDA)研究了9个土壤因子和14个地理气候因子与景天三七主要活性成分的关系,确定影响景天庚糖、谷甾醇、总黄酮和生物碱的主要生态因子,为景天三七的引种栽培提供技术依据。结果表明,景天三七各活性成分均存在明显的地域性差异,大荔引种区景天庚糖和谷甾醇的含量显著高于鄠邑和洛南引种区,洛南引种区的总黄酮含量又显著高于其他引种区。景天庚糖含量与谷甾醇之间呈极显著正相关(r=0.751),与生物碱和总黄酮呈负相关。土壤中铜(F=16.81,P=0.002)、钙(F=7.49,P=0.006)、铁(F=4.03,P=0.030)的含量及日温差(F=53.44,P=0.002)、平均气温(F=31.04,P=0.002)是造成景天三七活性成分差异的主要生态因子。景天庚糖和谷甾醇累积效应与土壤中钙含量、平均气温呈显著正相关,与速效钾、镁、锰、锌及温度因子呈正相关,与降水量呈负相关;生物碱含量对生态因子的响应与谷甾醇和景天庚糖总体相反;景天三七中总黄酮含量与日温差呈显著正相关,与土壤中锰、锌、有效磷、镁、钙、降雨量呈正相关,与温度因子呈负相关。产地的地理位置和生态因子是影响景天三七活性成分分化和形成的重要因素,关中东部大荔引种区土壤中钙含量高、气温较高,降水较少,有利于景天三七的活性成分的形成与累积,尤其以景天庚糖和谷甾醇含量增加最为明显,是景天三七适宜引种区。
Samples of Sedum aizoon were collected from 12 different fields of 3 introduction areas, the correlations between active components and ecological factors of Sedum aizoon were studied by correlation analysis, and the relationship between 9 soil factors,14 geographical climatic factors and 4 active components of Sedum aizoon were studied by redundancy analysis(RDA) to determine the main ecological factors affecting sedoheptulose, sitosterol, total flavonoids and alkaloids, which provided a technical basis for introduction and cultivation of Sedum aizoon. Results indicated that there were significant regional differences in the active constituents of Sedum aizoon(P<0.05). The content of sedoheptulose and stearol of Sedum aizoon collected from Dali was significantly higher than that collected from Huyi and Luonan. The total flavonoid content of Sedum aizoon which cultured in the introduction area of Luonan was significant higher than other areas. There was a significant positive correlation between the content of sedoheptulose and sitosterol(r=0.751), and a negative correlation between alkaloids and total flavonoids. The RDA results indicated that the contents of Cu(F=16.81, P=0.002), Ca(F=7.49, P=0.006) and Fe(F=4.03, P=0.030) in soil, as well as the daily temperature(F=53.44, P=0.002) and average temperature(F=31.04, P=0.002), was the main ecological factor causing the difference in active ingredients of Sedum aizoon. The cumulative effect of sedoheptulose and sitosterol was obviously positive correlated with soil Ca content and mean temperature, and positive correlated with available K, Mg, Mn, Zn and multiple temperature factors, and negatively correlated with precipitation. The response of alkaloids content to the ecological factors was opposite to that of sitosterol and sedoheptulose. The content of total flavonoids of Sedum aizoon was significantly positively correlated with the daily temperature,and was positive with Mn, Zn, available P, Mg, and Ca in soil and rainfall, while negatively correlated with multiple temperature factors. Thus, it is concluded that geographic position and ecological elements are main factors to determine the component differentiation and formation of Sedum aizoon. The central Shaanxi Plain is conducive to the formation and accumulation of active ingredients of Sedum aizoon, especially the increase of sedoheptulose and sitosterol, which due to more Ca in soil, the relatively higher average temperature and the relatively less precipitation. It is a suitable introduction area for Sedum aizoon L.
Samples of Sedum aizoon were collected from 12 different fields of 3 introduction areas, the correlations between active components and ecological factors of Sedum aizoon were studied by correlation analysis, and the relationship between 9 soil factors,14 geographical climatic factors and 4 active components of Sedum aizoon were studied by redundancy analysis(RDA) to determine the main ecological factors affecting sedoheptulose, sitosterol, total flavonoids and alkaloids, which provided a technical basis for introduction and cultivation of Sedum aizoon. Results indicated that there were significant regional differences in the active constituents of Sedum aizoon(P<0.05). The content of sedoheptulose and stearol of Sedum aizoon collected from Dali was significantly higher than that collected from Huyi and Luonan. The total flavonoid content of Sedum aizoon which cultured in the introduction area of Luonan was significant higher than other areas. There was a significant positive correlation between the content of sedoheptulose and sitosterol(r=0.751), and a negative correlation between alkaloids and total flavonoids. The RDA results indicated that the contents of Cu(F=16.81, P=0.002), Ca(F=7.49, P=0.006) and Fe(F=4.03, P=0.030) in soil, as well as the daily temperature(F=53.44, P=0.002) and average temperature(F=31.04, P=0.002), was the main ecological factor causing the difference in active ingredients of Sedum aizoon. The cumulative effect of sedoheptulose and sitosterol was obviously positive correlated with soil Ca content and mean temperature, and positive correlated with available K, Mg, Mn, Zn and multiple temperature factors, and negatively correlated with precipitation. The response of alkaloids content to the ecological factors was opposite to that of sitosterol and sedoheptulose. The content of total flavonoids of Sedum aizoon was significantly positively correlated with the daily temperature,and was positive with Mn, Zn, available P, Mg, and Ca in soil and rainfall, while negatively correlated with multiple temperature factors. Thus, it is concluded that geographic position and ecological elements are main factors to determine the component differentiation and formation of Sedum aizoon. The central Shaanxi Plain is conducive to the formation and accumulation of active ingredients of Sedum aizoon, especially the increase of sedoheptulose and sitosterol, which due to more Ca in soil, the relatively higher average temperature and the relatively less precipitation. It is a suitable introduction area for Sedum aizoon L.
引文
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李贺,刘世琦,王越,等,2013.钙对水培大蒜光合特性和品质的影响[J].园艺学报,40(6):1169-1177.LI H,LI S Q,WANG Y,et al.,2013.Effects of calcium on photosynthetic characteristics and quality of garlic[J].Acta Horticulturae Sinica,40(6):1169-1177.
李小伟,张宏涛,张泽婧,2018.西藏沙棘叶片黄酮含量与生态因子的相关性[J].生态环境学报,27(2):239-245.LI X W,ZHANG H T,ZHANG Z J,2018.The Relation between contents of flavonoids in leaves of Hippophae tibetana Schlecht.and ecological factors[J].Ecology and Environmental Sciences,27(2):239-245.
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