外源水杨酸对盐碱胁迫下燕麦抗氧化酶活性的影响
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摘要
为探究外源水杨酸对盐碱胁迫下燕麦抗氧化酶活性的影响,施加中性盐溶液和碱性盐溶液产生胁迫,并施加2种浓度外源水杨酸。结果表明:碱胁迫较盐胁迫显著抑制抗氧化酶活性;盐碱胁迫下抗性品种蒙燕一号抗氧化酶活性显著提高,丙二醛含量较品种太阳神少;施加外源水杨酸能显著提高抗氧化酶活性,对碱胁迫改善效果较好,0.05 mmol/L浓度较0.10 mmol/L浓度效果良好。
To explore the effect of exogenous salicylic acid on the antioxidant enzyme activities of Avena sativa L.under saline-alkali stress,a neutral salt solution and an alkaline salt solution were applied to generate stress followed by the application two concentrations of exogenous salicylic acid.The results showed that alkali stress significantly inhibits antioxidant enzyme activity compared with salt stress,and the antioxidant enzyme activity of the resistant variety "Monyan No.1" was significantly improved under salt-alkali stress.The content of malondialdehyde was less than that of the taiyangshen,application of exogenous salicylic acid significantly increased antioxidant enzyme activity,which had a better effect on improving alkali stress,and the 0.05 mmol/L concentration was more effective than the 0.1 mmol/L concentration.
To explore the effect of exogenous salicylic acid on the antioxidant enzyme activities of Avena sativa L.under saline-alkali stress,a neutral salt solution and an alkaline salt solution were applied to generate stress followed by the application two concentrations of exogenous salicylic acid.The results showed that alkali stress significantly inhibits antioxidant enzyme activity compared with salt stress,and the antioxidant enzyme activity of the resistant variety "Monyan No.1" was significantly improved under salt-alkali stress.The content of malondialdehyde was less than that of the taiyangshen,application of exogenous salicylic acid significantly increased antioxidant enzyme activity,which had a better effect on improving alkali stress,and the 0.05 mmol/L concentration was more effective than the 0.1 mmol/L concentration.
引文
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[18]张凤银,陈禅友,胡志辉,等.外源水杨酸对盐胁迫下菜豆种子萌发和幼苗生理特性的影响[J].东北农业大学学报,2013,44(10):39-43.
[19]孙丽娜,曲敏,任广涛,等.水杨酸对盐胁迫下黄瓜种子萌发和幼苗生长发育的影响[J].东北农业大学学报,2006,37(4):449-453.
[20]王玉萍,董雯,张鑫,等.水杨酸对盐胁迫下花椰菜种子萌发及幼苗生理特性的影响[J].草业学报,2012,21(1):213-219.
[21]陈颖,徐彩平,汪南阳,等.盐胁迫下水杨酸对南林895杨组培苗抗氧化系统的影响[J].南京林业大学学报,2012,36(6):17-22.
[22]李华,贺洪军,朱金英,等.外源水杨酸对黄瓜幼苗盐胁迫伤害的影响[J].北方园艺,2010(13):19-21.
[2]肖萍,仲伟鉴,张荣泉.SOD活性的化学发光和化学比色测定法的比较[J].上海预防医学杂志,1999,11(2):54-56.
[3]张志良,瞿伟菁.植物生理学实验指导[M].3版.北京:高等教育出报社,2003:123-124.
[4]赵世杰,许长成,邹琦,等.植物组织中丙二醛测定方法的改进[J].植物生理学通讯,1994,30(3):207-210.
[5]李璇,岳红,王升,等.影响植物抗氧化酶活性的因素及其研究热点和现状[J].中国中药杂志,2013,38(7):973-978.
[6]陈俊.碱地肤幼苗抗氧化酶系统对盐碱混合胁迫的生理响应特点[D].长春:东北师范大学,2006.
[7]KHARE T,KUMAR V,KISHOR P B.Na+and Cl-ions show additive effects under NaCl stress on induction of oxidative stress and the responsive antioxidative defense in rice[J].Protoplasma,2015,252(4):1149-1165.
[8]BRAY R C,COCKLE S A,FIELDEN E M,et al.Reduction and inactivation of superoxide dismutase by hydrogen peroxide[J].Biochemical Journal,1974,139(1):43-48.
[9]康爱平,刘艳,王殿,等.钾对能源植物杂交狼尾草耐盐性的影响[J].生态学报,2014,34(20):5793-5801.
[10]MANDHANIA S,MADAN S,SAWHNEY V.Antioxidant defense mechanism under salt stress in wheat seedlings[J].Biologia Plantarum,2006,50(2):227-231.
[11]SOHN Y G,LEE B H,KANG K Y,et al.Effects of NaCl stress on germination,antioxidant responses,and proline content in two rice cultivars[J].Journal of Plant Biology,2005,48(2):201-208.
[12]WANG X S,HAN J G.Changes of Proline Content,Activity,and Active Isoforms of Antioxidative Enzymes in Two Alfalfa Cultivars Under Salt Stress[J].Agricultural Sciences in China,2009,8(4):431-440.
[13]HU L,LI H,PANG H,et al.Responses of antioxidant gene,protein and enzymes to salinity stress in two genotypes of perennial ryegrass(Lolium perenne)differing in salt tolerance[J].Journal of Plant Physiology,2012,169(2):146-156.
[14]薛建平,王兴,张爱民,等.水杨酸对高温胁迫下半夏抗氧化系统的影响[J].中国中药杂志,2008,33(23):2847-2849.
[15]杜朝昆,李忠光,龚明.水杨酸诱导的玉米幼苗适应高温和低温胁迫的能力与抗氧化酶系统的关系[J].植物生理学通讯,2005(1):19-22.
[16]周旋.盐胁迫下茶树对外源水杨酸的生理响应[D].杨凌:西北农林科技大学,2014.
[17]张爱慧,朱士农.外源水杨酸对盐胁迫下丝瓜幼苗生长和抗氧化酶活性的影响[J].江西农业学报,2013,25(10):27-29.
[18]张凤银,陈禅友,胡志辉,等.外源水杨酸对盐胁迫下菜豆种子萌发和幼苗生理特性的影响[J].东北农业大学学报,2013,44(10):39-43.
[19]孙丽娜,曲敏,任广涛,等.水杨酸对盐胁迫下黄瓜种子萌发和幼苗生长发育的影响[J].东北农业大学学报,2006,37(4):449-453.
[20]王玉萍,董雯,张鑫,等.水杨酸对盐胁迫下花椰菜种子萌发及幼苗生理特性的影响[J].草业学报,2012,21(1):213-219.
[21]陈颖,徐彩平,汪南阳,等.盐胁迫下水杨酸对南林895杨组培苗抗氧化系统的影响[J].南京林业大学学报,2012,36(6):17-22.
[22]李华,贺洪军,朱金英,等.外源水杨酸对黄瓜幼苗盐胁迫伤害的影响[J].北方园艺,2010(13):19-21.
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