QuEChERS/超高效液相色谱-串联质谱法测定茶叶中17种氨基甲酸酯类农药及其代谢物残留

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英文篇名：Simultaneous determination of 17 carbamate pesticides residues in tea by QuEChERS-UPLC-MS/MS 作者：张艳侠 ; 张婷 ; 薛霞 ; 卢兰香 ; 杨昊 ; 周禹君 ; 祝建华 ; 刘艳明 英文作者：ZHANG Yan-xia;ZHANG Ting;XUE Xia;LU Lan-xiang;YANG Hao;ZHOU Yu-jun;ZHU Jian-hua;LIU Yan-ming;Shandong Institute for Food and Drug Control;Bioengineering Department,Shandong Polytechnic; 关键词：茶叶 ; 氨基甲酸酯类农药 ; QuEChERS ; 超高效液相色谱-串联质谱法(UPLC-MS/MS) 英文关键词：Tea;;Carbamate pesticide residues;;QuEChERS;;UPLC-MS/MS 中文刊名：分析试验室 英文刊名：Chinese Journal of Analysis Laboratory 机构：山东省食品药品检验研究院;山东职业学院生物工程系; 出版日期：2019-01-17 16:02 出版单位：分析试验室 年：2019 期：01 基金：国家重点研发计划(2017YFC1601600)资助 语种：中文; 页：18-23 页数：6 CN：11-2017/TF ISSN：1000-0720 分类号：O657.63;TS272.7

摘要

建立了检测茶叶中灭多威等17种氨基甲酸酯类农药残留的超高效液相色谱-串联质谱(UPLC-MS/MS)方法。详细讨论了加水对茶叶中氨基甲酸酯类农药提取效率的影响。茶叶样品加水后,用乙腈提取,经QuEChERS方法净化,C18色谱柱分离,三重四极杆电喷雾质谱检测,基质外标法定量。17种氨基甲酸酯类农药残留在各自质量浓度范围内线性关系良好,相关系数(R~2)均大于0. 99。方法检出限(S/N=3)为0. 001～0. 02 mg/kg,定量限(S/N=10)为0. 002～0. 05 mg/kg。在0. 05,0. 5,5. 0 mg/kg 3个添加水平下,17种农药残留平均加标回收率在82. 1%～119. 2%之间,相对标准偏差均小于8. 5%。
        A method for determination of 17 carbamate pesticide residues in tea by ultra performance liquid chromatography-tandem mass spectrometry( UPLC-MS/MS) was established. After rehydrated,the samples were extracted with acetonitrile and purified by the Qu ECh ERS method. The separation was carried out on a C18 column by ultra performance liquid chromatography( UPLC). The target compound was detected by MS/MS system with eletrospray ionization( ESI) under a multiple reaction monitoring( MRM) mode. The calibration curves of 17 carbamate pesticides showed good linearity in their own ranges with correlation coefficients higher than 0. 99. The average recoveries ranged from 82. 1% to 119. 2%,at three spiking levels with RSD < 8. 5%. The limit of detection( LOD) and the limit of quantitation( LOQ) of carbamate pesticide residues in tea were 0. 001 ～ 0. 02 mg/kg and 0. 002 ～ 0. 05 mg/kg,respectively.

引文

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