外泌体在流感病毒感染及其引发的肺部炎症中作用的研究进展
|
摘要
外泌体是细胞膜衍生出的一类通过在细胞间传递RNA和蛋白质来调控细胞间通讯的小囊泡。研究发现,当细胞受到压力释放外泌体后,可加重各种病理变化的进程,如病毒的感染过程。而外泌体microRNA(miRNA)在病毒感染及产生炎症时又起着调控作用。流感病毒感染是引起流行性感冒及病毒性肺炎的重要原因,感染机制复杂且尚未得到全面阐述。流感病毒可入侵人体呼吸道,多引发肺部炎症加重肺部损伤,甚至导致肺衰竭。本文总结目前研究进展,重点阐述了外泌体在流感病毒感染中的作用机制及对肺部炎症的影响,为后续研究提供了相关思路及理论指导,以期为流感病毒引起的疾病带来新的有效诊疗方法。
Exosomes are small vesicles derived from cell membranes that regulate intercellular communication by transmitting RNA and proteins between cells. Studies have shown that if cells are subjected to pressure to release exosomes,the progression of various pathological changes can be aggravated,such as the infection process of a virus. The microRNAs(miRNAs)in the exosome have a regulatory role in viral infection and inflammation. Influenza virus infection is an important cause of influenza and viral pneumonia. The infection mechanism is complex and has not been fully elaborated. Influenza viruses can invade the human respiratory tract,causing lung inflammation to aggravate lung damage and even lead to lung failure.This paper summarizes the current research progress,highlights the mechanism of action of exosomes in influenza virus infection and its impact on lung inflammation,to provide relevant ideas and theoretical guidance for follow-up research,with a view to bringing new effective treatment methods for diseases caused by influenza viruses.
Exosomes are small vesicles derived from cell membranes that regulate intercellular communication by transmitting RNA and proteins between cells. Studies have shown that if cells are subjected to pressure to release exosomes,the progression of various pathological changes can be aggravated,such as the infection process of a virus. The microRNAs(miRNAs)in the exosome have a regulatory role in viral infection and inflammation. Influenza virus infection is an important cause of influenza and viral pneumonia. The infection mechanism is complex and has not been fully elaborated. Influenza viruses can invade the human respiratory tract,causing lung inflammation to aggravate lung damage and even lead to lung failure.This paper summarizes the current research progress,highlights the mechanism of action of exosomes in influenza virus infection and its impact on lung inflammation,to provide relevant ideas and theoretical guidance for follow-up research,with a view to bringing new effective treatment methods for diseases caused by influenza viruses.
引文
[1] Bao Y, Gao Y, Jin Y, Cong W, Pan X, Cui X.MicroRNA expression profiles and networks in mouse lung infected with H1N1 influenza virus[J]. Mol Genet Genomics,2015,290:1885-1897.
[2] Buggele W A,Krause K E,Horvath C M. Small RNA profiling of influenza A virus-infected cells ientifies miR-449b as a regulator of histone deacetylase 1 and interferon beta[J/OL]. PLoS One,2013,8(9):e76560.
[3] Othumpangat S,Noti J D,Blachere F M,Beezhold D H. Expression of non-structural-1A binding protein in lung epithelial cells is modulated by miRNA-548an on exposure to influenza A virus[J]. Virology,2013,447(1-2):84-94.
[4] Yoon Y J,Kim O Y,Gho Y S. Extracellular vesicles as emerging intercellular communicasomes[J]. Bmb Rep,2014,47(10):531-539.
[5] Raposo G, Stoorvogel W. Extracellular vesicles:Exosomes,microvesicles,and friends[J]. J Cell Biol,2013,200(4):373-383.
[6] Gon Y,Maruoka S,Inoue T,Kuroda K,Yamagishi K,Kozu Y,Shikano S,Soda K,Lotvall J,Hashimoto S. Selective release of miRNAs via extracellular vesicles is associated with house dust mite allergen-induced airway inflammation[J]. Clin Exp Allergy,2017,47:1586-1598.,
[7] Yuan Z,Bedi B,Sadikot R T. Bronchoalveolar lavage exosomes in lipopolysaccharide-induced septic lung injury[J/OL]. J Vis Exp,2018,(135),e57737.
[8] Herold S, Becker C, Ridge K M, Budinger G R.Influenza virus-induced lung injury:Pathogenesis and implications for treatment[J]. Eur Respir J,2015,45(5):1463-1478.
[9] Shinya K,Ebina M,Yamada S,Ono M,Kasai N,Kawaoka Y. Avian flu:Influenza virus receptors in the human airway[J]. Nature,2006,440(7083):435-436.
[10]Gupta R, Radicioni G, Abdelwahab S, Dang H,Carpenter J,Chua M,Mieczkowski P A,Sheridan J T,Randell S H,Kesimer M. Intercellular communication between airway epithelial cells is mediated by exosomelike vesicles[J]. Am J Respir Cell Mol Biol,2019,60(2):209-220.
[11]Trobaugh D W,Klimstra W B. MicroRNA regulation of RNA virus replication and pathogenesis[J]. Trends Mol Med,2017,23(1):80-93.
[12]Makkoch J, Poomipak W, Saengchoowong S,Khongnomnan K, Praianantathavorn K, Jinato T,Poovorawan Y, Payungporn S. Human microRNAs profiling in response to influenza A viruses(subtypes pH1N1, H3N2, and H5N1)[J]. Exp Biol Med(Maywood),2016,241(4):409-420.
[13]Buggele W A,Johnson K E,Horvath C M. Influenza A virus infection of human respiratory cells induces primary microRNA expression[J]. J Biol Chem,2012,287(37):31027-1040.
[14]Peng S,Wang J,Wei S,Li C,Zhou K,Hu J,Ye X,Yan J, Liu W, Gao G F, Fang M, Meng S.Endogenous cellular microRNAs mediate antiviral defense against influenza A virus[J]. Mol Ther Nucleic Acids,2018,10:361-375.
[15]Kesimer M,Scull M,Brighton B,DeMaria G,Burns K, O'Neal W, Pickles R J, Sheehan J K.Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium:A possible role in innate defense[J]. FASEB J,2009,23(6):1858-1868.
[16]Scheller N,Herold S,Kellner R,Bertrams W,Jung L,Janga H,Greulich T,Schulte L N,Vogelmeier C F, Lohmeyer J, Schmeck B. Proviral microRNAs detected in extracellular vesicles from bronchoalveolar lavage fluid of patients with influenza virus-induced acute respiratory distress syndrome[J]. J Infect Dis,2019,219(4):540-543.
[17]Maemura T,Fukuyama S,Sugita Y,Lopes T J S,Nakao T, Noda T, Kawaoka Y. Lung-derived exosomal miR-483-3p regulates the innate immune response to influenza virus infection[J]. J Infect Dis,2018,217(9):1372-1382.
[18]Fujita Y,Kadota T,Araya,Ochiya T,Kuwano K.Extracellular vesicles:New players in lung immunity[J]. Am J Respir Cell Mol Biol,2018,58(5):560-565.
[19]Schneider C,Nobs S P,Heer A K,Kurrer M,Klinke G, van Rooijen N, Vogel J, Kopf M. Alveolar macrophages are essential for protection from respiratory failure and associated morbidity following influenza virus infection[J/OL]. PLoS Pathog, 2014, 10(4):e1004053.
[20]Moon H G,Cao Y,Yang J,Lee J H,Choi H S,Jin Y.Lung epithelial cell-derived extracellular vesicles activate macrophage-mediated inflammatory responses via ROCK1 pathway[J/OL]. Cell Death Dis,2015,6:e2016.
[21]Bonjoch L, Casas V, Carrascal M, Closa D.Involvement of exosomes in lung inflammation associated with experimental acute pancreatitis[J]. J Pathol,2016,240(2):235-245.
[22]Lee H, Zhang D, Zhu Z, Dela Cruz C S, Jin Y.Epithelialcell-derivedmicrovesiclesactivate macrophages and promote inflammation via microvesiclecontaining microRNAs[J]. Sci Rep,2016,6:35250.
[23]Zhang D,Lee H,Wang X,Rai A,Groot M,Jin Y.Exosome-mediated small RNA delivery:A novel therapeutic approach for inflammatory lung responses[J]. Mol Ther,2018,26(9):2119-2130.
[24]Kulshreshtha A, Ahmad T, Agrawal A, Ghosh B.Proinflammatory role of epithelial cell–derived exosomes in allergic airway inflammation[J]. J Allergy Clin Immunol,2013,131(4):1194-1203.
[2] Buggele W A,Krause K E,Horvath C M. Small RNA profiling of influenza A virus-infected cells ientifies miR-449b as a regulator of histone deacetylase 1 and interferon beta[J/OL]. PLoS One,2013,8(9):e76560.
[3] Othumpangat S,Noti J D,Blachere F M,Beezhold D H. Expression of non-structural-1A binding protein in lung epithelial cells is modulated by miRNA-548an on exposure to influenza A virus[J]. Virology,2013,447(1-2):84-94.
[4] Yoon Y J,Kim O Y,Gho Y S. Extracellular vesicles as emerging intercellular communicasomes[J]. Bmb Rep,2014,47(10):531-539.
[5] Raposo G, Stoorvogel W. Extracellular vesicles:Exosomes,microvesicles,and friends[J]. J Cell Biol,2013,200(4):373-383.
[6] Gon Y,Maruoka S,Inoue T,Kuroda K,Yamagishi K,Kozu Y,Shikano S,Soda K,Lotvall J,Hashimoto S. Selective release of miRNAs via extracellular vesicles is associated with house dust mite allergen-induced airway inflammation[J]. Clin Exp Allergy,2017,47:1586-1598.,
[7] Yuan Z,Bedi B,Sadikot R T. Bronchoalveolar lavage exosomes in lipopolysaccharide-induced septic lung injury[J/OL]. J Vis Exp,2018,(135),e57737.
[8] Herold S, Becker C, Ridge K M, Budinger G R.Influenza virus-induced lung injury:Pathogenesis and implications for treatment[J]. Eur Respir J,2015,45(5):1463-1478.
[9] Shinya K,Ebina M,Yamada S,Ono M,Kasai N,Kawaoka Y. Avian flu:Influenza virus receptors in the human airway[J]. Nature,2006,440(7083):435-436.
[10]Gupta R, Radicioni G, Abdelwahab S, Dang H,Carpenter J,Chua M,Mieczkowski P A,Sheridan J T,Randell S H,Kesimer M. Intercellular communication between airway epithelial cells is mediated by exosomelike vesicles[J]. Am J Respir Cell Mol Biol,2019,60(2):209-220.
[11]Trobaugh D W,Klimstra W B. MicroRNA regulation of RNA virus replication and pathogenesis[J]. Trends Mol Med,2017,23(1):80-93.
[12]Makkoch J, Poomipak W, Saengchoowong S,Khongnomnan K, Praianantathavorn K, Jinato T,Poovorawan Y, Payungporn S. Human microRNAs profiling in response to influenza A viruses(subtypes pH1N1, H3N2, and H5N1)[J]. Exp Biol Med(Maywood),2016,241(4):409-420.
[13]Buggele W A,Johnson K E,Horvath C M. Influenza A virus infection of human respiratory cells induces primary microRNA expression[J]. J Biol Chem,2012,287(37):31027-1040.
[14]Peng S,Wang J,Wei S,Li C,Zhou K,Hu J,Ye X,Yan J, Liu W, Gao G F, Fang M, Meng S.Endogenous cellular microRNAs mediate antiviral defense against influenza A virus[J]. Mol Ther Nucleic Acids,2018,10:361-375.
[15]Kesimer M,Scull M,Brighton B,DeMaria G,Burns K, O'Neal W, Pickles R J, Sheehan J K.Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium:A possible role in innate defense[J]. FASEB J,2009,23(6):1858-1868.
[16]Scheller N,Herold S,Kellner R,Bertrams W,Jung L,Janga H,Greulich T,Schulte L N,Vogelmeier C F, Lohmeyer J, Schmeck B. Proviral microRNAs detected in extracellular vesicles from bronchoalveolar lavage fluid of patients with influenza virus-induced acute respiratory distress syndrome[J]. J Infect Dis,2019,219(4):540-543.
[17]Maemura T,Fukuyama S,Sugita Y,Lopes T J S,Nakao T, Noda T, Kawaoka Y. Lung-derived exosomal miR-483-3p regulates the innate immune response to influenza virus infection[J]. J Infect Dis,2018,217(9):1372-1382.
[18]Fujita Y,Kadota T,Araya,Ochiya T,Kuwano K.Extracellular vesicles:New players in lung immunity[J]. Am J Respir Cell Mol Biol,2018,58(5):560-565.
[19]Schneider C,Nobs S P,Heer A K,Kurrer M,Klinke G, van Rooijen N, Vogel J, Kopf M. Alveolar macrophages are essential for protection from respiratory failure and associated morbidity following influenza virus infection[J/OL]. PLoS Pathog, 2014, 10(4):e1004053.
[20]Moon H G,Cao Y,Yang J,Lee J H,Choi H S,Jin Y.Lung epithelial cell-derived extracellular vesicles activate macrophage-mediated inflammatory responses via ROCK1 pathway[J/OL]. Cell Death Dis,2015,6:e2016.
[21]Bonjoch L, Casas V, Carrascal M, Closa D.Involvement of exosomes in lung inflammation associated with experimental acute pancreatitis[J]. J Pathol,2016,240(2):235-245.
[22]Lee H, Zhang D, Zhu Z, Dela Cruz C S, Jin Y.Epithelialcell-derivedmicrovesiclesactivate macrophages and promote inflammation via microvesiclecontaining microRNAs[J]. Sci Rep,2016,6:35250.
[23]Zhang D,Lee H,Wang X,Rai A,Groot M,Jin Y.Exosome-mediated small RNA delivery:A novel therapeutic approach for inflammatory lung responses[J]. Mol Ther,2018,26(9):2119-2130.
[24]Kulshreshtha A, Ahmad T, Agrawal A, Ghosh B.Proinflammatory role of epithelial cell–derived exosomes in allergic airway inflammation[J]. J Allergy Clin Immunol,2013,131(4):1194-1203.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |