截形叶螨对阿维·哒螨灵的抗性遗传方式研究
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摘要
【目的】明确截形叶螨Tetranychus truncatus Ehara对阿维·哒螨灵药剂的抗性遗传方式,为今后合理使用药剂,有效地制定害螨抗性治理策略提供一定的理论指导。【方法】通过杂交和回交试验,确定截形叶螨抗阿维·哒螨灵种群的显性度(D)、细胞质影响因子和遗传基因。【结果】所测得的截形叶螨抗性种群的杂交F1代(SS♀?RR♂和RR♀SS♂)的显性度D值分别为0.625和0.802,均在0 0.05),证明截形叶螨对阿维·哒螨灵的抗性遗传受母体或核外效应的影响不大,其抗性基因在常染色体上;回交F2代两个种群的(BC1SR和BC1RS)的实际死亡率和期望值经χ2检验,均差异显著,证实由多基因控制。【结论】不完全显性多基因控制的遗传方式产生的抗谱很宽,抗性一旦产生则很难延缓其抗性的迅速发展,因此田间应用该药剂时应交替轮换不同作用机制的药剂,重点采用预防性抗性治理策略。
[Objectives] To clarify the inheritance of resistance to avermectin+pyridaben in Tetranychus truncatus Ehara.[Methods] Resistant(RR)and susceptible(SS) strains of Tetranychus truncatus Ehara were crossed and backcrossed to investigate the genes involved in resistance to avermectin+pyridaben, their dominance(D) and cytoplasmic factors involved in resistance. [Results] D values for F1 females from SS♀?RR♂ crosses and RR♀?SS♂ crosses were in the range of 0 < D < 1(0.625 and 0.802, respectively), indicating incomplete dominance. The 95% confidence limits of these two D values overlap,indicating that there is no significant difference between DSR and DRS. This reveals that the gene responsible for resistance is located in the autosome. A Chi-square(χ2) goodness-of-fit analysis indicated that mortality levels in F2 females(BC1 SR and BC1 RS) were significantly different(χ2= 16.92, df = 9, P < 0.05) from the expected values for multiple gene control.[Conclusion] The inheritance of resistance to abamectin+pyridaben in T. truncatus can easily cause a wide spectrum of resistance that is difficult to reduce. Consequently, abamectin+pyridaben should be used in rotation with the other insecticides to reduce selection for resistance to this combination of insecticides. It's important to implement a preventive resistance management strategy and measures to manage resistance to abamectin+pyridaben.
[Objectives] To clarify the inheritance of resistance to avermectin+pyridaben in Tetranychus truncatus Ehara.[Methods] Resistant(RR)and susceptible(SS) strains of Tetranychus truncatus Ehara were crossed and backcrossed to investigate the genes involved in resistance to avermectin+pyridaben, their dominance(D) and cytoplasmic factors involved in resistance. [Results] D values for F1 females from SS♀?RR♂ crosses and RR♀?SS♂ crosses were in the range of 0 < D < 1(0.625 and 0.802, respectively), indicating incomplete dominance. The 95% confidence limits of these two D values overlap,indicating that there is no significant difference between DSR and DRS. This reveals that the gene responsible for resistance is located in the autosome. A Chi-square(χ2) goodness-of-fit analysis indicated that mortality levels in F2 females(BC1 SR and BC1 RS) were significantly different(χ2= 16.92, df = 9, P < 0.05) from the expected values for multiple gene control.[Conclusion] The inheritance of resistance to abamectin+pyridaben in T. truncatus can easily cause a wide spectrum of resistance that is difficult to reduce. Consequently, abamectin+pyridaben should be used in rotation with the other insecticides to reduce selection for resistance to this combination of insecticides. It's important to implement a preventive resistance management strategy and measures to manage resistance to abamectin+pyridaben.
引文
Ay R,Kara FE,2014.Toxicity,inheritance and biochemistry of clofentezine resistance in Tetranychus urticae.Insect Science,18(10):503-511.
Bai YL,2005.The various mechanism of acaricides.Modern Agrochemicals,4(3):27-30.[柏亚罗,2005.具有不同作用机理的杀螨剂.现代农药,4(3):27-30.]
FAO,1980.Revised method for spider mites and their eggs(e.g.Tetranychus spp.and Panonychus ulmi Koch).FAO Plant Production and Protection,21:49-54.
Ferreira CBS,Andrade FHN,Rodrigues ARS,Siqueira HAA,Gondim MGC,2015.Resistance in field populations of Tetranychus urticae to acaricides and characterization of the inheritance of abamectin resistance.Crop Protection,67(1):77-83.
Georghiou GP,Garber MJ,1965.Studies on the inheritance of carbamate-resistance in the housefly(Musca domestica).Bulletin of the World Health Organization,32(2):181.
He L,Gao X,Wang J,Zhao Z,Liu N,2009.Genetic analysis of abamectin resistance in Tetranychus cinnabarinus.Pesticide Biochemistry&Physiology,95(3):147-151.
He L,Zhao ZM,Cao XF,Deng XP,Wang JJ,2005.Resistance selection of Tetranychus cinnabarinus to fenpropathrin and genetic analysis.Scientia Agricultura Sinica,38(4):719-724.[何林,赵志模,曹小芳,邓新平,王进军,2005.朱砂叶螨抗甲氰菊酯品系选育及遗传分析.中国农业科学,38(4):719-724.]
He SW,Zhang JC,Wang ZH,Gao YM,2017.The formula study of avermectin-pyridaben microemulsion.Gansu Agricultural Science and Technology,(12):58-60.[何树文,张建朝,王泽浩,高宜明,2017.不同药剂处理对制种玉米叶螨的防效.甘肃农业科技,(12):58-60.]
Liu QX,Wu YQ,1994.Review on inheritance of pesticide resistance in phytophagous spider mites.Hereditas,16(5):45-48.[刘芹轩,武予清,1994.植食叶螨抗药性遗传学研究的进展.遗传,16(5):45-48.]
Preisler HK,Hoy MA,Robertson JL,1990.Statistical analysis of modes of inheritance for pesticide resistance.Journal of Economic Entomology,83(5):1649-1655.
Song LW,Li MW,Shen HM,2016.Selection for pyridaben,avermectin and abamectin+pyridaben resistance in Tetranychus truncatus Ehara.Chinese Journal of Applied Entomology,53(1):89-94.[宋丽雯,李妙雯,沈慧敏,2016.截形叶螨对哒螨灵、阿维菌素和阿维·哒螨灵的抗性选育和抗性稳定性研究.应用昆虫学报,53(1):89-94.]
Stone BF,1968.A formula for determining degree of dominance in cases of monofactorial inheritance of resistance to chemicals.Bulletin of the World Health Organization,38(2):325.
Uesugi R,Goka K,Osakabe M,2002.Genetic basis of resistances to chlorfenapyr and etoxazole in the two-spotted spider mite(Acari:Tetranychidae).Journal of Economic Entomology,95(6):1267-1274.
Van Leeuwen T,Tirry L,Yamamoto A,Nauen R,Dermauw W,2015.The economic importance of acaricides in the control of phytophagous mites and an update on recent acaricide mode of action research.Pesticide Biochemistry&Physiology,121(6):12-21.
Van Pottelberge S,Van Leeuwen T,Nauen R,Tirry L,2009.Resistance mechanisms to mitochondrial electron transport inhibitors in a field-collected strain of Tetranychus urticae Koch(Acari:Tetranychidae).Bulletin of Entomological Research,99(1):23-31.
Wang Y,Lu HR,2017.Patent overview of avermectin structural modification.Statistics&Analysis,14(5):21-37.[万玥,陆皞然,2017.基于阿维菌素结构衍生的专利技术综述.中国发明与专利,14(5):21-37.]
Wang ZH,Wu QJ,Xu BY,Zhang YJ,2011.Laboratory selection and inheritance mode of resistance to spinosad in the western flower thrips,Frankliniella occidentalis.Chinese Journal of Applied Entomology,48(3):553-558.[王泽华,吴青君,徐宝云,张友军,2011.西花蓟马对多杀菌素的抗性汰选和遗传方式.应用昆虫学报,48(3):553-558.]
Zhao BS,Li JC,2003.The study on avermectin+pyridaben microemulsion.Jiangsu Chemical Industry,21(2):44-46.[赵宝顺,李嘉诚,2003.阿维菌素-哒螨灵微乳剂的研究.江苏化工,21(2):44-46.]
Bai YL,2005.The various mechanism of acaricides.Modern Agrochemicals,4(3):27-30.[柏亚罗,2005.具有不同作用机理的杀螨剂.现代农药,4(3):27-30.]
FAO,1980.Revised method for spider mites and their eggs(e.g.Tetranychus spp.and Panonychus ulmi Koch).FAO Plant Production and Protection,21:49-54.
Ferreira CBS,Andrade FHN,Rodrigues ARS,Siqueira HAA,Gondim MGC,2015.Resistance in field populations of Tetranychus urticae to acaricides and characterization of the inheritance of abamectin resistance.Crop Protection,67(1):77-83.
Georghiou GP,Garber MJ,1965.Studies on the inheritance of carbamate-resistance in the housefly(Musca domestica).Bulletin of the World Health Organization,32(2):181.
He L,Gao X,Wang J,Zhao Z,Liu N,2009.Genetic analysis of abamectin resistance in Tetranychus cinnabarinus.Pesticide Biochemistry&Physiology,95(3):147-151.
He L,Zhao ZM,Cao XF,Deng XP,Wang JJ,2005.Resistance selection of Tetranychus cinnabarinus to fenpropathrin and genetic analysis.Scientia Agricultura Sinica,38(4):719-724.[何林,赵志模,曹小芳,邓新平,王进军,2005.朱砂叶螨抗甲氰菊酯品系选育及遗传分析.中国农业科学,38(4):719-724.]
He SW,Zhang JC,Wang ZH,Gao YM,2017.The formula study of avermectin-pyridaben microemulsion.Gansu Agricultural Science and Technology,(12):58-60.[何树文,张建朝,王泽浩,高宜明,2017.不同药剂处理对制种玉米叶螨的防效.甘肃农业科技,(12):58-60.]
Liu QX,Wu YQ,1994.Review on inheritance of pesticide resistance in phytophagous spider mites.Hereditas,16(5):45-48.[刘芹轩,武予清,1994.植食叶螨抗药性遗传学研究的进展.遗传,16(5):45-48.]
Preisler HK,Hoy MA,Robertson JL,1990.Statistical analysis of modes of inheritance for pesticide resistance.Journal of Economic Entomology,83(5):1649-1655.
Song LW,Li MW,Shen HM,2016.Selection for pyridaben,avermectin and abamectin+pyridaben resistance in Tetranychus truncatus Ehara.Chinese Journal of Applied Entomology,53(1):89-94.[宋丽雯,李妙雯,沈慧敏,2016.截形叶螨对哒螨灵、阿维菌素和阿维·哒螨灵的抗性选育和抗性稳定性研究.应用昆虫学报,53(1):89-94.]
Stone BF,1968.A formula for determining degree of dominance in cases of monofactorial inheritance of resistance to chemicals.Bulletin of the World Health Organization,38(2):325.
Uesugi R,Goka K,Osakabe M,2002.Genetic basis of resistances to chlorfenapyr and etoxazole in the two-spotted spider mite(Acari:Tetranychidae).Journal of Economic Entomology,95(6):1267-1274.
Van Leeuwen T,Tirry L,Yamamoto A,Nauen R,Dermauw W,2015.The economic importance of acaricides in the control of phytophagous mites and an update on recent acaricide mode of action research.Pesticide Biochemistry&Physiology,121(6):12-21.
Van Pottelberge S,Van Leeuwen T,Nauen R,Tirry L,2009.Resistance mechanisms to mitochondrial electron transport inhibitors in a field-collected strain of Tetranychus urticae Koch(Acari:Tetranychidae).Bulletin of Entomological Research,99(1):23-31.
Wang Y,Lu HR,2017.Patent overview of avermectin structural modification.Statistics&Analysis,14(5):21-37.[万玥,陆皞然,2017.基于阿维菌素结构衍生的专利技术综述.中国发明与专利,14(5):21-37.]
Wang ZH,Wu QJ,Xu BY,Zhang YJ,2011.Laboratory selection and inheritance mode of resistance to spinosad in the western flower thrips,Frankliniella occidentalis.Chinese Journal of Applied Entomology,48(3):553-558.[王泽华,吴青君,徐宝云,张友军,2011.西花蓟马对多杀菌素的抗性汰选和遗传方式.应用昆虫学报,48(3):553-558.]
Zhao BS,Li JC,2003.The study on avermectin+pyridaben microemulsion.Jiangsu Chemical Industry,21(2):44-46.[赵宝顺,李嘉诚,2003.阿维菌素-哒螨灵微乳剂的研究.江苏化工,21(2):44-46.]
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