不同地区成体大熊猫肠道微生物结构差异性及其与纤维素消化能力的相关性
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摘要
肠道微生物对大熊猫的消化吸收和代谢免疫具有重要的意义.为了解上海、四川两地成体大熊猫肠道微生物结构的差异及其与消化纤维素能力的关系,对两地大熊猫肠道微生物多样性、纤维素酶活性以及两地环境差异、食物性质等进行分析,并对成体大熊猫肠道微生物性质与纤维素酶活性进行相关性分析.结果表明,上海成体大熊猫肠道菌群结构较四川成体大熊猫更加丰富,两地成体大熊猫肠道优势菌和特异性菌有较大差异.上海成体大熊猫具有更高的纤维素消化能力,这可能得益于其肠道中具有更丰富的细菌多样性和较丰富的芽孢杆菌属(Bacillus)及山野壳菌科未知菌属(Unclassified_f_Montagnulaceae).两地成体大熊猫肠道微生物多样性及消化纤维素能力的差异,可能是由食物性质、海拔等外在环境因素导致的.总体而言,不同地区成体大熊猫肠道微生物结构差异明显,且对大熊猫肠道的消化能力具有一定的影响.(图3表7参37)
The giant panda is one of the rarest species in our country. People focus on raising, breeding, and conservation of giant pandas. The intestinal microorganisms are very important for giant pandas' digestion, absorption, metabolism, and immunity. The purpose of this study was to investigate the differences in the intestinal microbial structure of adult giant pandas between Shanghai and Sichuan and the relationship with their ability to digest cellulose. The gut microbial diversity and cellulase activity of giant pandas, as well as the environmental differences and food properties in two places were analyzed.The correlation of the microbial properties and cellulase activities of adult giant pandas were analyzed. The microbial diversity was greater in the gut of adult giant pandas in Shanghai, and the dominant bacteria and unique genus of giant pandas in Shanghai were vastly different than that in Sichuan pandas. Moreover, the cellulose digestibility was higher in Shanghai because of its richer bacterial diversity and more abundant Bacillus and Unclassified_f_Montagnulaceae. The differences in the intestinal microbial structure and cellulose digestive abilities of adult giant pandas might be affected by some external environmental factors, such as food and attitude. Overall, the difference in the intestine microbial diversity of adult giant pandas in different regions could partly affect cellulolytic capacity.
The giant panda is one of the rarest species in our country. People focus on raising, breeding, and conservation of giant pandas. The intestinal microorganisms are very important for giant pandas' digestion, absorption, metabolism, and immunity. The purpose of this study was to investigate the differences in the intestinal microbial structure of adult giant pandas between Shanghai and Sichuan and the relationship with their ability to digest cellulose. The gut microbial diversity and cellulase activity of giant pandas, as well as the environmental differences and food properties in two places were analyzed.The correlation of the microbial properties and cellulase activities of adult giant pandas were analyzed. The microbial diversity was greater in the gut of adult giant pandas in Shanghai, and the dominant bacteria and unique genus of giant pandas in Shanghai were vastly different than that in Sichuan pandas. Moreover, the cellulose digestibility was higher in Shanghai because of its richer bacterial diversity and more abundant Bacillus and Unclassified_f_Montagnulaceae. The differences in the intestinal microbial structure and cellulose digestive abilities of adult giant pandas might be affected by some external environmental factors, such as food and attitude. Overall, the difference in the intestine microbial diversity of adult giant pandas in different regions could partly affect cellulolytic capacity.
引文
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21袁楠,亢宗静,卢圣鄂,王莹燕,张小平,辜运富.富集培养下的若尔盖高原湿地低温纤维素降解细菌群落结构[J].应用与环境生物学报,2016,22(3):402-408[Yuan N,Kang ZJ,Lu SE,Wang YY,Zhang XP,Gu YF.Community structures of the cold-adapted cellulosedegrading bacteria in the Zoige plateau wetland under enrichment culture conditions[J].Chin J Appl Environ Biol,2016,22(3):402-408]
22曾文慧,钟俊鸿,李秋健,刘炳荣.反应温度对台湾乳白蚁内外源纤维素酶活力的差异影响[J].江苏农业科学,2017,45(8):91-94[Zeng WH,ZhongJH,Li QJ,Liu BR.Effects of reaction temperature on endogenous and exogenous cellulase activities of C.formosanus in Taiwan[J].Jiangsu Agric Sci,2017,45(8):91-94.]
23骆伦伦.秸秆对黄粉虫生长发育、消化酶和肠道微生物的影响[D].杭州:浙江农林大学,2017[Luo LL.Effects of straw on growth,development,digestive enzymes and intestinal microbes of Tenebrio molitor[D].Hangzhou:Zhejiang A&F University,2017]
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25 Justyna MC.Transcriptome remodeling of Pseudomonas putida KT2440during mcl-PHAs synthesis:effect of different carbon sources and response to nitrogen stress[J].J Ind Microbiol Biotechnol,2018,45(6):1-14
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2 Guo L.PGRP-SC2 promotes gut immune homeostasis to limit commensal dysbiosis and extend lifespan[J].Cell,2014,156(1-2):109-122
3 Chu H.Gene-microbiota interactions contribute to the pathogenesis of inflammatory bowel disease[J].Science,2016,352(6289):1116
4 Perry RJ.Acetate mediates a microbiome-brain-βcell axis promoting metabolic syndrome[J].Nature,2016,534(7606):213-217
5 Rangan KJ.A secreted bacterial peptidoglycan hydrolase enhances tolerance to enteric pathogens.Science,2016,353(6306):1434
6 Shao Y.Symbiont-derived antimicrobials contribute to the control of the lepidopteran gut microbiota[J].Cell Chem Biol,2017,24(1):66
7 Hesla HM.Impact of lifestyle on the gut microbiota of healthy infants and their mothers-the ALADDIN birth cohort[J].FEMS Microbiol Ecol,2015,90(3):791-801
8 Gohir W.Pregnancy-related changes in the maternal gut microbiota are dependent upon the mother’s periconceptional diet[J].Gut Microb,2015,6(5):310-320
9朱立猛.菊粉对小鼠肠道微生物调节作用的研究[D].北京:中国科学院大学,2017[Zhu LM.Study on intestinal microorganism regulation effect of inulin on mice[D].Beijing:University of Chinese Academy of Science,2017]
10 Ohland CL.Effects of Lactobacillus helveticus on murine behavior are dependent on diet and genotype and correlate with alterations in the gut microbiome[J].Psychoneuroendocrinology,2013,38(9):1738-174
11何永果,晋蕾,李果,李才武,李蓓,李伟,张亚辉,胡正泉,黄炎,张和民,邹立扣.基于高通量测序技术研究成年大熊猫肠道菌群[J].应用与环境生物学报,2017,23(5):771-777[He YG,Jin L.Li G,Li CW,Li B,Li W,Zhang YH,Hu ZQ,Huang Y,Zhang HM,Zou LK.Gut microbiome of adult giant pandas based on high-throughput sequencing technology[J].Chin J Appl Environ Biol,2017,23(5):771-777]
12李蓓,郭莉娟,龙梅,汤纯香,周晓平,罗燕,邹立扣.圈养大熊猫肠道微生物分离、鉴定及细菌耐药性研究[J].四川动物,2014,33(2):161-166[Li B,Guo LJ,Long M,Tang CX,Zhou XP,Luo Y,Zou LK.Isolation and antimicrobial resistance of bacteria from intestinal tract of giant panda[J].Sichuan J Zool,2014,33(2):161-166]
13曹涵文,吴珑韬,甘乾福,鲍若虹,梁学武.熊猫粪便中纤维素降解菌的筛选与鉴定[J].家畜生态学报,2015,36(6):19-25[Cao HW,Wu LT,Gan QF,Bao RH,Liang XW.Isolation and Identification of cellulosedegradation bacteria from panda dung[J].Acta Ecol Anim Dom,2015,36(6):19-25.]
14马缨,殷红涛.大熊猫源纤维素分解菌的分离及产酶条件研究[J].黑龙江畜牧兽医,2017(2):172-174[Ma Y,Yin HY.Isolation and enzyme production of giant panda derived cellulolytic bacteria[J].Heilongjiang Ani Husb,2017(2):172-174]
15张文平,张志和.大熊猫肠道宏基因组发育分析[R].第十三届全国野生动物生态与资源保护学术研讨会暨第六届中国西部动物学学术研讨会,成都,2017[Zhang WP,Zhang ZH.Macro genome development analysis of giant panda’s intestine[R].The 13th National Symposium on Wildlife Conservation and the 6th Western China Zoological Symposium,Chengdu,2017]
16房伟,方泽民,张学成,肖亚中.肠道微生物协助大熊猫转化降解木质素的证据[R].全国微生物资源学术暨国家微生物资源平台运行服务研讨会,呼和浩特,2012[Fang W,Fang ZM,Zhang XC,Xiao YZ.Intestinal microorganisms assisting giant pandas in transforming and degrading lignin[R].National Microbial Resources Academic and National Microbial Resource Platform Operation Service Seminar,Huhhot,2012]
17 Li R.Erratum:the sequence and de novo assembly of the giant panda genome[J].Nature,2010,463:311-317
18陆海燕,宋美,史卫东,郭铁.蒽酮光度法测定羧甲基纤维素钠[J].天津科技大学学报,1996(2):86-88[Lu HY,Song M,Shi WD,Guo T.Anthrone photometric on the determination of sodium carboxymethyl cellulose[J].J Tianjin Univ Light Ind,1996(2):86-88]
19范鹏程,田静,黄静美,雷文泉,邱会东.花生壳中纤维素和木质素含量的测定方法[J].重庆科技学院学报:自然科学版,2008,10(5):64-65.[Fan PC,Tian J,Huang JM,Lei WQ,Qiu HD.On the determination of cellulose and lignin of peanut shells[J].J Chongqing Univ Sci Technol Nat Sci Ed,2008,10(5):64-65.]
20徐昶,龙敏南,邬小兵,徐惠娟,陈重安,张凤章,许良树.高产纤维素酶菌株的筛选及产酶条件研究[J].厦门大学学报(自然版),2005,44(1):107-111[Xu C,Long MN,Wu XB,Xu HJ,Chen CA,Zhang FZ,Xu LS.Screening and characterization of a high cellulase producing strain Aspergillus glaucus XC9[J].J Xiamen Univ(Nat Sci),2005,44(1):107-111]
21袁楠,亢宗静,卢圣鄂,王莹燕,张小平,辜运富.富集培养下的若尔盖高原湿地低温纤维素降解细菌群落结构[J].应用与环境生物学报,2016,22(3):402-408[Yuan N,Kang ZJ,Lu SE,Wang YY,Zhang XP,Gu YF.Community structures of the cold-adapted cellulosedegrading bacteria in the Zoige plateau wetland under enrichment culture conditions[J].Chin J Appl Environ Biol,2016,22(3):402-408]
22曾文慧,钟俊鸿,李秋健,刘炳荣.反应温度对台湾乳白蚁内外源纤维素酶活力的差异影响[J].江苏农业科学,2017,45(8):91-94[Zeng WH,ZhongJH,Li QJ,Liu BR.Effects of reaction temperature on endogenous and exogenous cellulase activities of C.formosanus in Taiwan[J].Jiangsu Agric Sci,2017,45(8):91-94.]
23骆伦伦.秸秆对黄粉虫生长发育、消化酶和肠道微生物的影响[D].杭州:浙江农林大学,2017[Luo LL.Effects of straw on growth,development,digestive enzymes and intestinal microbes of Tenebrio molitor[D].Hangzhou:Zhejiang A&F University,2017]
24段云峰,吴晓丽,金锋.饮食对自闭症的影响研究进展[J].科学通报,2015,60(30):2845-2861[Duan YF,Wu XL,Jin F.Advances in research on the effects of diet on autism[J].Chin Sci Bull,2015,60(30):2845-2861]
25 Justyna MC.Transcriptome remodeling of Pseudomonas putida KT2440during mcl-PHAs synthesis:effect of different carbon sources and response to nitrogen stress[J].J Ind Microbiol Biotechnol,2018,45(6):1-14
26步长英.丝兰皂甙、芽孢杆菌对肉鸡生产性能、氮代谢和排泄物氨气散发量的影响[D].泰安:山东农业大学,2008[Bu CY.Effect of saponin and Bacillus on broiler performance,nitrogen metabolism and excretion ammonia emissions[D].Tai’an:Shandong Agricultural University,2008]
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