西藏色季拉山毛壳属及形态相似属真菌的多样性及系统发育分析
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摘要
从西藏色季拉山不同海拔采集的323份标本中分离毛壳属(Chaetomium)及形态相似属真菌,共分离到29个菌株,通过形态学特征将其中的27个菌株分别鉴定为3属5种:球毛壳(C.globosum)、马德拉斯毛壳(C.madrasense)、近缘毛壳(C.subaffine)、绳生二叉毛壳(Dichotomopilus funicola)和粗壮围领毛壳(Colariella robusta).通过ITS rDNA和28SrDNA D1/D2区序列构建系统发育树,分离的29个菌株被划分为6个种.碱基变异分析结果表明,ITS rDNA序列较28SrDNA D1/D2区序列变异更大,结合这两段基因序列可进行毛壳属及形态相似属真菌属内种间的系统发育研究.这些结果可丰富西藏毛壳属及形态相似属真菌资源库,为开发毛壳属及形态相似属真菌代谢产物奠定基础.
323 samples were collected from different altitudes of Sejila Mountains in Tibet,and 29 strains of Chaetomium and Chaetomium-like fungi were isolated.The results showed that 27 strains of them were identified as 3 genera 5 species,C.globosum,C.madrasense,C.subaffine,Dichotomopilus funicola and Colariella robusta.Phylogenetic tree was constructed based on sequences of ITS rDNA and 28 SrDNA D1/D2 region,showing that the 29 isolated strains were divided into 6 groups.The results of base mutation analysis showed that the ITS rDNA region had more variations than that of the 28 SrDNA D1/D2 region,and the combination with these two gene sequences could be used in the phylogenetic study between species within genus of Chaetomiumand Chaetomium-like fungi.Taken together,this study enriched the resources of Chaetomiumand Chaetomium-like fungi in Tibet and laid a foundation for exploring metabolites of Chaetomiumand Chaetomium-like fungi.
323 samples were collected from different altitudes of Sejila Mountains in Tibet,and 29 strains of Chaetomium and Chaetomium-like fungi were isolated.The results showed that 27 strains of them were identified as 3 genera 5 species,C.globosum,C.madrasense,C.subaffine,Dichotomopilus funicola and Colariella robusta.Phylogenetic tree was constructed based on sequences of ITS rDNA and 28 SrDNA D1/D2 region,showing that the 29 isolated strains were divided into 6 groups.The results of base mutation analysis showed that the ITS rDNA region had more variations than that of the 28 SrDNA D1/D2 region,and the combination with these two gene sequences could be used in the phylogenetic study between species within genus of Chaetomiumand Chaetomium-like fungi.Taken together,this study enriched the resources of Chaetomiumand Chaetomium-like fungi in Tibet and laid a foundation for exploring metabolites of Chaetomiumand Chaetomium-like fungi.
引文
[1]KIRK P M,CANNON P F,DAVID J C,et al.Ainsworth&Bisby’s dictionary of the fungi[M].9th ed.Wallingford:CAB International,2001:610-614.
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[3]陈庆涛.毛壳菌和壳针孢菌的新种[J].微生物学报,1973(2):124-128.
[4]戴芳澜.中国真菌总汇[M].北京:科学技术出版社,1979.
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[9]李凯,袁鹤.植物病害生物防治概述[J].山西农业科学,2012,40(7):807-810.
[10]印容,高慧娟,赵秀云.球毛壳菌及其产生的鞘氨醇对油菜根肿病的室内生防作用[J].华中农业大学学报,2016,35(5):58-62.
[11]宋富海,王森,张先富,等.球毛壳ND35菌肥对苹果连作土壤微生物和平邑甜茶幼苗生物量的影响[J].园艺学报,2015,42(2):205-213.
[12]丛国强,尹成林,何邦令,等.水分胁迫下内生真菌球毛壳ND35对冬小麦苗期生长和抗旱性的影响[J].生态学报,2015,35(18):1-13.
[13]夏宣宣,张淑勇,张光灿,等.连作土壤中施加球毛壳ND35菌肥对杨树生理特性的影响[J].应用生态学报,2016,27(7):2249-2256.
[14]辛雅芬,米士伟,张淑玲,等.球毛壳菌在黄瓜上的侵染定殖及其对黄瓜生长和产量的影响[J].吉林农业大学学报,2013,35(4):450-456.
[15]GREIF M D,STCHIGE L A M,HUHNDOR F S M.Are-evaluation of genus Chaetomidiumbased on molecular and morphological characters[J].Mycologia,2009,101:554-564.
[16]ASGARI B,ZARE R.The genus Chaetomiumin Iran,a phylogenetic study including six new species[J].Mycologia,2011,103:863-882.
[17]WANG X W,HOUBRAKEN J,GROENEWALD J Z,et al.Diversity and taxonomy of Chaetomium and Chaetomium-like fungi from indoor environments[J].Studies in Mycology,2016,84:145-224.
[18]徐阿生,罗建.中国盘菌属Peziza一新记录种[J].菌物学报,2007,26(1):148-149.
[19]张惠,范宇光,图力古尔.采自西藏的盔孢菌属2个中国新记录种[J].东北林业大学学报,2012,40(5):134-135.
[20]蔡晓布,彭岳林,盖京苹,等.藏北高寒草原土壤活性有机碳对AM真菌物种多样性的影响[J].农业工程学报,2012,28(5):216-221.
[21]戴玉成,庄剑云.中国菌物已知种属[J].菌物学报,2010,29(5):625-628.
[22]DAI Y C,CUI B K,SI J,et al.Dynamics of the worldwide number of fungi with emphasis on fungal diversity in China[J].Mycological Progress,2015,14:62.
[23]刘小娇,徐阿生,邓丽君.西藏色季拉山区红菇科资源[J].中国食用菌,2010,29(4):8-9.
[24]邓丽君,徐阿生,刘小娇.西藏色季拉山丝膜菌属真菌资源[J].中国食用菌,2011,30(5):6-7.
[25]刘述春,孙炳达,旺姆,等.一株毛壳霉属真菌中新结构活性聚酮类化合物研究[J].菌物学报,2010,29(5):726-731.
[26]GUO Y Z,ZHU M Q,SUN G Y.The family Chaetomiaceae from China 4.Two newly recorded species of Chaetomium[J].Mycotaxon,2011,116:247-251.
[27]ARX J A,VON G J,FIGUERAS M J.The Ascomycete genus Chaetomium[J].Nova Hedwigia Beiheft,1986,84:1-162.
[28]NAKADA M,TANAKA C,TSUNEWAKI K.RFLPanalysis for species separation in the genera Bipolaris and Curvularis[J].Mycoscience,1994,35:271-278.
[29]WANG X W,WANG X L,LIU F J,et al.Phylogenetic assessment of Chaetomium indicum and allied species,with the introduction of three new species and epitypification of C.funicola and C.indicum[J].Mycological Progress,2014,13(3):719-732.
[30]张桂珍.玉米大斑病菌生理小种鉴定及球毛壳菌对其生防作用研究[D].长春:吉林大学,2012:1-69.
[31]SCHOCH C L,SEIFERT K A,HUHNDORF S,et al.Nuclear ribosomal internal transcribed spacer(ITS)region as a universal DNA barcode marker for fungi[J].Proceedings of National Academy of Sciences of the United States of America,2012,109(16):6241-6246.
[32]杨成龙,陈章娥,吴小平,等.基因组ITS序列分析鉴定红曲霉菌株[J].核农学报,2015,29(2):252-259.
[2]KIRK P M,CANNON P F,MINTER D W,et al.Ainsworth&Bisby’s dictionary of the fungi[M].10th ed.Wallingford:CAB International,2008:420-450.
[3]陈庆涛.毛壳菌和壳针孢菌的新种[J].微生物学报,1973(2):124-128.
[4]戴芳澜.中国真菌总汇[M].北京:科学技术出版社,1979.
[5]孙广宇,谭悠久,张荣.中国毛壳菌科研究Ⅰ.毛壳菌属的种[J].菌物学报,2004,23(3):333-337.
[6]谭悠久.毛壳菌科(Chaetomiace)分类及分子系统发育研究[D].杨凌:西北农林科技大学,2005:1-93.
[7]王雪薇.中国毛壳菌属Chaetomium Kunze及其形态相似类群的系统分析研究[D].北京:中国科学院大学,2005.
[8]刘富江.中国部分省(区)毛壳菌属真菌的分类研究[D].乌鲁木齐:新疆农业大学,2009:1-93.
[9]李凯,袁鹤.植物病害生物防治概述[J].山西农业科学,2012,40(7):807-810.
[10]印容,高慧娟,赵秀云.球毛壳菌及其产生的鞘氨醇对油菜根肿病的室内生防作用[J].华中农业大学学报,2016,35(5):58-62.
[11]宋富海,王森,张先富,等.球毛壳ND35菌肥对苹果连作土壤微生物和平邑甜茶幼苗生物量的影响[J].园艺学报,2015,42(2):205-213.
[12]丛国强,尹成林,何邦令,等.水分胁迫下内生真菌球毛壳ND35对冬小麦苗期生长和抗旱性的影响[J].生态学报,2015,35(18):1-13.
[13]夏宣宣,张淑勇,张光灿,等.连作土壤中施加球毛壳ND35菌肥对杨树生理特性的影响[J].应用生态学报,2016,27(7):2249-2256.
[14]辛雅芬,米士伟,张淑玲,等.球毛壳菌在黄瓜上的侵染定殖及其对黄瓜生长和产量的影响[J].吉林农业大学学报,2013,35(4):450-456.
[15]GREIF M D,STCHIGE L A M,HUHNDOR F S M.Are-evaluation of genus Chaetomidiumbased on molecular and morphological characters[J].Mycologia,2009,101:554-564.
[16]ASGARI B,ZARE R.The genus Chaetomiumin Iran,a phylogenetic study including six new species[J].Mycologia,2011,103:863-882.
[17]WANG X W,HOUBRAKEN J,GROENEWALD J Z,et al.Diversity and taxonomy of Chaetomium and Chaetomium-like fungi from indoor environments[J].Studies in Mycology,2016,84:145-224.
[18]徐阿生,罗建.中国盘菌属Peziza一新记录种[J].菌物学报,2007,26(1):148-149.
[19]张惠,范宇光,图力古尔.采自西藏的盔孢菌属2个中国新记录种[J].东北林业大学学报,2012,40(5):134-135.
[20]蔡晓布,彭岳林,盖京苹,等.藏北高寒草原土壤活性有机碳对AM真菌物种多样性的影响[J].农业工程学报,2012,28(5):216-221.
[21]戴玉成,庄剑云.中国菌物已知种属[J].菌物学报,2010,29(5):625-628.
[22]DAI Y C,CUI B K,SI J,et al.Dynamics of the worldwide number of fungi with emphasis on fungal diversity in China[J].Mycological Progress,2015,14:62.
[23]刘小娇,徐阿生,邓丽君.西藏色季拉山区红菇科资源[J].中国食用菌,2010,29(4):8-9.
[24]邓丽君,徐阿生,刘小娇.西藏色季拉山丝膜菌属真菌资源[J].中国食用菌,2011,30(5):6-7.
[25]刘述春,孙炳达,旺姆,等.一株毛壳霉属真菌中新结构活性聚酮类化合物研究[J].菌物学报,2010,29(5):726-731.
[26]GUO Y Z,ZHU M Q,SUN G Y.The family Chaetomiaceae from China 4.Two newly recorded species of Chaetomium[J].Mycotaxon,2011,116:247-251.
[27]ARX J A,VON G J,FIGUERAS M J.The Ascomycete genus Chaetomium[J].Nova Hedwigia Beiheft,1986,84:1-162.
[28]NAKADA M,TANAKA C,TSUNEWAKI K.RFLPanalysis for species separation in the genera Bipolaris and Curvularis[J].Mycoscience,1994,35:271-278.
[29]WANG X W,WANG X L,LIU F J,et al.Phylogenetic assessment of Chaetomium indicum and allied species,with the introduction of three new species and epitypification of C.funicola and C.indicum[J].Mycological Progress,2014,13(3):719-732.
[30]张桂珍.玉米大斑病菌生理小种鉴定及球毛壳菌对其生防作用研究[D].长春:吉林大学,2012:1-69.
[31]SCHOCH C L,SEIFERT K A,HUHNDORF S,et al.Nuclear ribosomal internal transcribed spacer(ITS)region as a universal DNA barcode marker for fungi[J].Proceedings of National Academy of Sciences of the United States of America,2012,109(16):6241-6246.
[32]杨成龙,陈章娥,吴小平,等.基因组ITS序列分析鉴定红曲霉菌株[J].核农学报,2015,29(2):252-259.
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