小球藻适宜培养营养盐以及添加单糖对生长的影响

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英文篇名：The Effect of Chlorella on the Cultivation of Nutrients and the Effect of Adding Monosaccharides on Growth 作者：刘秀花 ; 梁梁 ; 杜风光 ; 王有名 ; 梁峰 英文作者：LIU Xiuhua;LIANG Liang;DU Fengguang;WANG Youming;LIANG Feng;Biorefinery Engineering Lab of Henan Province, Shangqiu Normal University;State Key Laboratory of Motor Vehicle Biofuel Technology; 关键词：小球藻 ; 木糖 ; 葡萄糖 ; 薄层层析 英文关键词：Chlorella;;xylose;;glucose;;thin layer chromatography 中文刊名：河南科技 英文刊名：Henan Science and Technology 机构：商丘师范学院生物精炼河南省工程实验室;车用生物燃料技术国家重点实验室; 出版日期：2019-06-15 出版单位：河南科技 年：2019 期：17 基金：车用生物燃料技术国家重点实验室开放基金资助项目(KFKT2014018) 语种：中文; 页：140-145 页数：6 CN：41-1081/T ISSN：1003-5168 分类号：Q949.9

摘要

木糖为自然界中最丰富和经济的糖资源之一,但木糖很少被证明是野生型微藻的碳源。本实验的目的是确定小球藻的最适培养基以及对糖的具体利用情况。通过对小球藻干重和培养后的藻液中糖含量的测定发现,在HSM培养基中,小球藻生长较好,最适pH为6.5。添加木糖对小球藻的生长存在一定的抑制作用,随着糖含量的增加,抑制越来越严重。在木糖含量扩大培养中发现,木糖耐受在6g/L左右。之后进行混合糖(木糖和葡萄糖)培养小球藻,确定混合糖培养效果明显好于单糖培养;经过薄层层析和DNS对培养后的藻液进行测定,结果发现,有葡萄糖存在时,小球藻优先利用葡萄糖。
        Xylose is one of the most abundant and economical sugar resources in nature, but xylose is rarely proven to be a carbon source for wild-type microalgae. The purpose of this experiment was to determine the optimum medium for Chlorella and the specific use of sugar. By measuring the dry weight of the Chlorella and the sugar content in the algae after the culture, it was found that the Chlorella grew well in the HSM medium, and the optimum pH was 6.5.The addition of xylose had a certain inhibition on the growth of Chlorella, and as the sugar content increases, the inhibition became more and more serious. In the expansion of xylose content, xylose tolerance was found to be around 6 g/L. After the cultivation of Chlorella with mixed sugar(xylose and glucose), it could be confirmed that the mixed sugar culture effect was significantly better than the monosaccharide culture, and the cultured algae liquid was measured by thin layer chromatography and DNS, and it was found that there was glucose present. Chlorella preferentially utilized glucose.

引文

[1]Kapdan I K,Fikret Kargi. Bio-hydrogen production from waste materials[J]. Enzyme&Microbial Technology,2006(5):569-582.
    [2]Yang C,Hua Q,Shimizu K. Energetics and carbon metabolism during growth of microalgal cells under photoautotrophic, mixotrophic and cyclic light-autotrophic/dark-heterotrophic conditions[J]. Biochemical Engineering Journal,2000(2):87-102.
    [3]Bumbak F,Stella Cook,Vilém Zachleder,et al. Best practices in heterotrophic high-cell-density microalgal processes:achievements, potential and possible limitations[J]. Applied Microbiology&Biotechnology,2011(1):31-46.
    [4]Neish A. C. Carbohydrate nutrition of Chlorella vulgaris[J]. Can J Bot,1951(29):68-78.
    [5]Samejima H,Myers J. On the Heterotrophic Growth of Chlorella pyrenoidosa[J]. Journal of General Microbiology,1958(1):107.
    [6]Hassall K A. Xylose as a Specific Inhibitor of Photosynthesis[J]. Nature,1958(4618):1273-1274.
    [7]刘秀花，崔孟岐，王莉，等.双向薄层色谱法分析混合糖组分[J].商丘师范学院学报，2015(6):69-73.
    [8]Miller G L. Use of dinitrosalicylic acid reagent for determination of reducing sugars[J]. Analytical Chemistry,1959(3):426-428.
    [9]刘秀花，王莉，张淑红，等.薄层色谱法分析玉米芯降解液糖类成分优化研究[J].商丘师范学院学报，2014(9):55-59.