痕量氨基酸分析方法研究进展
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摘要
本文评述了近年来痕量氨基酸(AAs)的分析检测方法,包括气相色谱法(GC)、高效液相色谱法(HPLC)、毛细管电泳法(CE)和直接检测法,以及已经商业化的氨基酸分析仪法。重点介绍了基于通用型仪器方法所涉及的色谱条件、衍生化试剂,讨论了衍生化试剂类型、稳定性、潜在危害性、衍生化时间等衍生化过程中的关键影响步骤,以及商业化的AAs分析仪法。通过综合分析上述方法的优缺点,发现对于各种不同基体中痕量AAs的分析,高效液相色谱柱前衍生法和AAs分析仪法是优先选择。
Trace amino acids play an important role in the fields of environment, food, biology, medicine, etc. Due to their wide variety, complex matrix and selective signal response, the rapid and accurate analysis of trace amino acids is difficult but urgent. This paper reviews the analytical methods for the determination of trace amino acids in recent years, including gas chromatography, high performance liquid chromatography, capillary electrophoresis and direct detection based on general-purpose instruments, as well as the commercially available amino acid analyzer. The chromatographic conditions, derivatization reagents, corresponding detectors and detection limits based on the general instrument method are introduced. The key influence steps in the derivatization process such as the type of derivatization reagent, stability, potential hazard and derivatization time are discussed. By comprehensively considering the advantages and disadvantages of the above methods, it is found that the high-performance liquid chromatography pre-column derivatization method and the amino acid analyzer are preferred for the determination of trace amino acids in various matrix, and can provide effective reference for analysis and detection of trace amino acids in unknown samples.
Trace amino acids play an important role in the fields of environment, food, biology, medicine, etc. Due to their wide variety, complex matrix and selective signal response, the rapid and accurate analysis of trace amino acids is difficult but urgent. This paper reviews the analytical methods for the determination of trace amino acids in recent years, including gas chromatography, high performance liquid chromatography, capillary electrophoresis and direct detection based on general-purpose instruments, as well as the commercially available amino acid analyzer. The chromatographic conditions, derivatization reagents, corresponding detectors and detection limits based on the general instrument method are introduced. The key influence steps in the derivatization process such as the type of derivatization reagent, stability, potential hazard and derivatization time are discussed. By comprehensively considering the advantages and disadvantages of the above methods, it is found that the high-performance liquid chromatography pre-column derivatization method and the amino acid analyzer are preferred for the determination of trace amino acids in various matrix, and can provide effective reference for analysis and detection of trace amino acids in unknown samples.
引文
[1] Roccaro P,Vagliasindi F G A,KorShin G V.Water Supply Res Technol Aqua,2014,50:1.
[2] Jin D,Miyahara T,Oe T,et al.Analytical Biochemistry,1999,269(1):124.
[3] Csapó J,Schmidt J,Csapó-Kiss Z,et al.Chromatographia,2001,56(1):S169.
[4] Gandolfi I,Palla G,Marchelli R,et al.Journal of Food Science,2010,59(1):152.
[5] Song Y,Xu C,KuRoki H,et al.Journal of Pharmaceutical & Biomedical Analysis,2018,147:35.
[6] YU H,MU S F.Chinese Journal of Analytical Chemistry(于泓,牟世芬.分析化学),2005,22(3):398.
[7] XING J,LI Q L,GENG T H,et al.China Food Additives(邢健,李巧玲,耿涛华,等.中国食品添加剂),2012,(5):187.
[8] Menestrina F,Grisales J O,Castells C B.Microchemical Journal,2016,128:267.
[9] Khuhawar M Y,Majidano S A.Chromatographia,2011,73(7-8):701.
[10] Waldhier M C,Dettmer K,Gruber M A,et al.Journal of Chromatography B,2010,878(16):1103.
[11] Paredes R M,Pinto C G,Pavón J L,et al.Journal of Separation Science,2016,39(17):3375.
[12] Podea P.Analytical Letters,2015,48(1):37.
[13] HUANG Z,LONG S,SHA Y F,et al.Chinese Journal of Analysis Laboratory(黄志,龙帅,沙云菲,等.分析试验室),2017,(11):1341.
[14] HOU J,LEI W,DU X N,et al.Journal of Instrumental Analysis(侯捷,雷雯,杜晓宁,等.分析测试学报),2016,35(9):1132.
[15] Guo M,Shi T,Duan Y,et al.Journal of Food Composition & Analysis,2015,42:84.
[16] Shi Z,Li H,Li Z,et al.Ieri Procedia,2013,5(84):351.
[17] ZHAO Y L,YAN D H,LI Y.Food Science(赵英莲,牟德华,李艳.食品科学),2015,36(6):178.
[18] Liyanaarachchi G,Mahanama K,Somasiri H,et al.Food Chemistry,2017,240:196.
[19] Zhang L,Li Y,Zhou H,et al.Journal of Pharmaceutical & Biomedical Analysis,2012,66(1):356.
[20] Zhu Y,Luo Y,Wang P,et al.Food Chemistry,2016,194:643.
[21] YANG Z,YANG L L,ZHU W B,et al.Chinese Journal of Clinical Pharmacology and Therapeutics(杨智,阳利龙,祝文兵,等.中国临床药理学与治疗学),2011,16(5):549.
[22] Li Y,Tang A G,Mu S,et al.Clinica Chimica Acta,2011,412(11-12):1032.
[23] Wu J L,Yu S Y,Wu S H,et al.Neuroscience Letters,2016,616:32.
[24] Tang W,Li X,Wu X,et al.Open Journal of Social Sciences,2013,01(6):23.
[25] Wang M,Shen J.Chinese Pharmacology,2013,22(3):239.
[26] YANG B M,YAO L X,GUO B,et al.Food Science(杨苞梅,姚丽贤,国彬,等.食品科学),2011,32(16):249.
[27] SUN Y C,XU X Z,XU Y L,et al.Chinese Journal of Chromatography(孙言春,许宪祝,徐衍岭,等.色谱),2013,31(3):275.
[28] SU J K,WANG X,LU J Q,et al.Chinese Journal of Experimental Traditional Medical Formulae(苏建坤,王雪,卢建秋,等.中国实验方剂学杂志),2012,18(15):135.
[29] LI Q,LI G,ZHANG H N.Food and Nutrition in China(李琪,李广,张会妮.中国食物与营养),2013,19(2):68.
[30] MIAO Y T,YANG Y Y,WANG H,et al.Journal of Food Safety & Quality(苗雨田,杨悠悠,王浩,等.食品安全质量检测学报),2015,(4):1154.
[31] AIZEZ M H M T,SHA L N,ABULIMITI Y L.China Pharmacy(艾则孜·莫合买提,沙丽娜,阿布力米提·伊力.中国药房),2017,28(12):1680.
[32] LI Y,QIU G Y,SHI X F,et al.Chinese Journal of Pharmaceutical Analysis(李运,邱国玉,石晓峰,等.药物分析杂志),2017,(6):988.
[33] LI Q,LI G,ZHANG H N.Food and Nutrition in China(李琪,李广,张会妮.中国食物与营养),2013,19(2):68.
[34] SHI J L,LI Y,YAN T,et al.Chinese Journal of Analysis Laboratory(师君丽,李勇,逄涛,等.分析试验室),2013,32(5):57.
[35] ZHOU X Y,SHI Y P.Chinese Journal of Chromatography(周贤婧,师彦平.色谱),2013,31(7):661.
[36] DING H M,GE E N,XU J D.Chinese Journal of Experimental Traditional Medical Formulae(丁红梅,葛尔宁,许家栋.中国实验方剂学杂志),2013,19(2):117.
[37] HU Y F,HUANG Z Q,LI J F.Journal of Instrumental Analysis(胡月芳,黄志强,李金芳.分析测试学报),2016,35(4):471.
[38] TONG P.Analysis and Testing Technology and Instruments(童萍.分析测试技术与仪器),2014,(4):197-203.
[39] Zhao D,Lu M,Cai Z.Electrophoresis,2012,33(15):2424.
[40] Wagner Z,Tábi T,Jakó T,et al.Analytical & Bioanalytical Chemistry,2012,404(8):2363.
[41] Lorenzo M P,Navarrete A,Balderas C,et al.Journal of Pharmaceutical & Biomedical Analysis,2013,73(2):116.
[42] REN T Y,QIU J,XU Z Q.Chinese Journal of Analysis Laboratory(任挺钧,邱俊,徐中其.分析试验室),2017,(6).693.
[43] WANG Y F,CAI Y L,LIN X,et al.Chemical Research and Application(王宇飞,蔡元丽,林夏,等.化学研究与应用),2013,25(3):311.
[44] Li,Ruiting & Liu,Pei fang,et al.Amino Acids,2016,48(6):1523.
[45] Zuo T H,Bu setti F,Linge K L,et al.Journal of Chromatography A,2014,1370:135.
[46] ZHENG Z,SUN Q,SHI Y W,et al.Chinese Journal of Chromatography(郑重,孙琦,石永伟,等.色谱),2015,33(3):309.
[47] CHEN X Y,PU P,KANG J Q,et al.Journal of Northwest Sci-Tech University of A & F(Natural Science Edition)(陈惜燕,蒲鹏,康靖全,等.西北农林科技大学学报:自然科学版),2017,45(5):183.
[48] ZHONG T H,HUANG L Y,FANG J.Analysis and Testing Technology and Instrument(钟添华,黄丽英,房静.分析测试技术与仪器),2011,17(2):74.
[49] YU H X,XU H T,MA Q,et al.Chinese Tobacco Science(于宏晓,徐海涛,马强,等.中国烟草科学),2012,(4):51.
[50] WANG X F,CHEN M F,LIU J J,et al.Journal of Analytical Science(王晓芬,陈美芬,刘俊俊,等.分析科学学报),2016,32(3):330.
[51] Li X,Xiao D,Sanders T,Liu Y.Analytical & Bioanalytical Chemistry,2013,405(25):8131.
[52] Atherton T,Croxton R,Baron M.Journal of Separation Science,2012,35(21):2994.
[53] LI P F,TAO W,ZHANG X D,et al.Chinese Journal of Analytical Chemistry(李鹏飞,陶蓓蓓,张绪得,等.分析化学),2013,41(9):1347.
[54] LIN X J,XU Y X,LIU X H,et al.China Brewing(林小娟,许杨彪,刘向红,等.中国酿造),2017,36(6):158.
[55] MO R H,TANG F B,DING M,et al.Chemistry(莫润宏,汤富彬,丁明,等.化学通报),2012,75(12):1126.
[56] CHEN X,LI H M,WANG J,et al.The Food Industry(陈霞,李红敏,王静,等.食品工业),2015,(2):255.
[57] JIA P Q,SUN R,YAN F,et al.Chinese Journal of Biologicals(贾鹏禹,孙蕊,寇芳,等.中国生物制品学杂志),2018,31(08):869.
[58] WANG H J,ZHOU X Q,FENG Z Q,et al.Modern Food Science and Technology(王洪健,周兴起,冯志强,等.现代食品科技),2012,28(3):348.
[59] WANG J,PAN X Y,YANG H W.Chinese Journal of Pharmaceutical Analysis(王棘,潘雪妍,杨宏伟.药物分析杂志),2012,(6):1085.
[60] Shim Y S,Yoon W J,Ha J,et al.Food Science & Biotechnology,2013,22(6):1567.
[2] Jin D,Miyahara T,Oe T,et al.Analytical Biochemistry,1999,269(1):124.
[3] Csapó J,Schmidt J,Csapó-Kiss Z,et al.Chromatographia,2001,56(1):S169.
[4] Gandolfi I,Palla G,Marchelli R,et al.Journal of Food Science,2010,59(1):152.
[5] Song Y,Xu C,KuRoki H,et al.Journal of Pharmaceutical & Biomedical Analysis,2018,147:35.
[6] YU H,MU S F.Chinese Journal of Analytical Chemistry(于泓,牟世芬.分析化学),2005,22(3):398.
[7] XING J,LI Q L,GENG T H,et al.China Food Additives(邢健,李巧玲,耿涛华,等.中国食品添加剂),2012,(5):187.
[8] Menestrina F,Grisales J O,Castells C B.Microchemical Journal,2016,128:267.
[9] Khuhawar M Y,Majidano S A.Chromatographia,2011,73(7-8):701.
[10] Waldhier M C,Dettmer K,Gruber M A,et al.Journal of Chromatography B,2010,878(16):1103.
[11] Paredes R M,Pinto C G,Pavón J L,et al.Journal of Separation Science,2016,39(17):3375.
[12] Podea P.Analytical Letters,2015,48(1):37.
[13] HUANG Z,LONG S,SHA Y F,et al.Chinese Journal of Analysis Laboratory(黄志,龙帅,沙云菲,等.分析试验室),2017,(11):1341.
[14] HOU J,LEI W,DU X N,et al.Journal of Instrumental Analysis(侯捷,雷雯,杜晓宁,等.分析测试学报),2016,35(9):1132.
[15] Guo M,Shi T,Duan Y,et al.Journal of Food Composition & Analysis,2015,42:84.
[16] Shi Z,Li H,Li Z,et al.Ieri Procedia,2013,5(84):351.
[17] ZHAO Y L,YAN D H,LI Y.Food Science(赵英莲,牟德华,李艳.食品科学),2015,36(6):178.
[18] Liyanaarachchi G,Mahanama K,Somasiri H,et al.Food Chemistry,2017,240:196.
[19] Zhang L,Li Y,Zhou H,et al.Journal of Pharmaceutical & Biomedical Analysis,2012,66(1):356.
[20] Zhu Y,Luo Y,Wang P,et al.Food Chemistry,2016,194:643.
[21] YANG Z,YANG L L,ZHU W B,et al.Chinese Journal of Clinical Pharmacology and Therapeutics(杨智,阳利龙,祝文兵,等.中国临床药理学与治疗学),2011,16(5):549.
[22] Li Y,Tang A G,Mu S,et al.Clinica Chimica Acta,2011,412(11-12):1032.
[23] Wu J L,Yu S Y,Wu S H,et al.Neuroscience Letters,2016,616:32.
[24] Tang W,Li X,Wu X,et al.Open Journal of Social Sciences,2013,01(6):23.
[25] Wang M,Shen J.Chinese Pharmacology,2013,22(3):239.
[26] YANG B M,YAO L X,GUO B,et al.Food Science(杨苞梅,姚丽贤,国彬,等.食品科学),2011,32(16):249.
[27] SUN Y C,XU X Z,XU Y L,et al.Chinese Journal of Chromatography(孙言春,许宪祝,徐衍岭,等.色谱),2013,31(3):275.
[28] SU J K,WANG X,LU J Q,et al.Chinese Journal of Experimental Traditional Medical Formulae(苏建坤,王雪,卢建秋,等.中国实验方剂学杂志),2012,18(15):135.
[29] LI Q,LI G,ZHANG H N.Food and Nutrition in China(李琪,李广,张会妮.中国食物与营养),2013,19(2):68.
[30] MIAO Y T,YANG Y Y,WANG H,et al.Journal of Food Safety & Quality(苗雨田,杨悠悠,王浩,等.食品安全质量检测学报),2015,(4):1154.
[31] AIZEZ M H M T,SHA L N,ABULIMITI Y L.China Pharmacy(艾则孜·莫合买提,沙丽娜,阿布力米提·伊力.中国药房),2017,28(12):1680.
[32] LI Y,QIU G Y,SHI X F,et al.Chinese Journal of Pharmaceutical Analysis(李运,邱国玉,石晓峰,等.药物分析杂志),2017,(6):988.
[33] LI Q,LI G,ZHANG H N.Food and Nutrition in China(李琪,李广,张会妮.中国食物与营养),2013,19(2):68.
[34] SHI J L,LI Y,YAN T,et al.Chinese Journal of Analysis Laboratory(师君丽,李勇,逄涛,等.分析试验室),2013,32(5):57.
[35] ZHOU X Y,SHI Y P.Chinese Journal of Chromatography(周贤婧,师彦平.色谱),2013,31(7):661.
[36] DING H M,GE E N,XU J D.Chinese Journal of Experimental Traditional Medical Formulae(丁红梅,葛尔宁,许家栋.中国实验方剂学杂志),2013,19(2):117.
[37] HU Y F,HUANG Z Q,LI J F.Journal of Instrumental Analysis(胡月芳,黄志强,李金芳.分析测试学报),2016,35(4):471.
[38] TONG P.Analysis and Testing Technology and Instruments(童萍.分析测试技术与仪器),2014,(4):197-203.
[39] Zhao D,Lu M,Cai Z.Electrophoresis,2012,33(15):2424.
[40] Wagner Z,Tábi T,Jakó T,et al.Analytical & Bioanalytical Chemistry,2012,404(8):2363.
[41] Lorenzo M P,Navarrete A,Balderas C,et al.Journal of Pharmaceutical & Biomedical Analysis,2013,73(2):116.
[42] REN T Y,QIU J,XU Z Q.Chinese Journal of Analysis Laboratory(任挺钧,邱俊,徐中其.分析试验室),2017,(6).693.
[43] WANG Y F,CAI Y L,LIN X,et al.Chemical Research and Application(王宇飞,蔡元丽,林夏,等.化学研究与应用),2013,25(3):311.
[44] Li,Ruiting & Liu,Pei fang,et al.Amino Acids,2016,48(6):1523.
[45] Zuo T H,Bu setti F,Linge K L,et al.Journal of Chromatography A,2014,1370:135.
[46] ZHENG Z,SUN Q,SHI Y W,et al.Chinese Journal of Chromatography(郑重,孙琦,石永伟,等.色谱),2015,33(3):309.
[47] CHEN X Y,PU P,KANG J Q,et al.Journal of Northwest Sci-Tech University of A & F(Natural Science Edition)(陈惜燕,蒲鹏,康靖全,等.西北农林科技大学学报:自然科学版),2017,45(5):183.
[48] ZHONG T H,HUANG L Y,FANG J.Analysis and Testing Technology and Instrument(钟添华,黄丽英,房静.分析测试技术与仪器),2011,17(2):74.
[49] YU H X,XU H T,MA Q,et al.Chinese Tobacco Science(于宏晓,徐海涛,马强,等.中国烟草科学),2012,(4):51.
[50] WANG X F,CHEN M F,LIU J J,et al.Journal of Analytical Science(王晓芬,陈美芬,刘俊俊,等.分析科学学报),2016,32(3):330.
[51] Li X,Xiao D,Sanders T,Liu Y.Analytical & Bioanalytical Chemistry,2013,405(25):8131.
[52] Atherton T,Croxton R,Baron M.Journal of Separation Science,2012,35(21):2994.
[53] LI P F,TAO W,ZHANG X D,et al.Chinese Journal of Analytical Chemistry(李鹏飞,陶蓓蓓,张绪得,等.分析化学),2013,41(9):1347.
[54] LIN X J,XU Y X,LIU X H,et al.China Brewing(林小娟,许杨彪,刘向红,等.中国酿造),2017,36(6):158.
[55] MO R H,TANG F B,DING M,et al.Chemistry(莫润宏,汤富彬,丁明,等.化学通报),2012,75(12):1126.
[56] CHEN X,LI H M,WANG J,et al.The Food Industry(陈霞,李红敏,王静,等.食品工业),2015,(2):255.
[57] JIA P Q,SUN R,YAN F,et al.Chinese Journal of Biologicals(贾鹏禹,孙蕊,寇芳,等.中国生物制品学杂志),2018,31(08):869.
[58] WANG H J,ZHOU X Q,FENG Z Q,et al.Modern Food Science and Technology(王洪健,周兴起,冯志强,等.现代食品科技),2012,28(3):348.
[59] WANG J,PAN X Y,YANG H W.Chinese Journal of Pharmaceutical Analysis(王棘,潘雪妍,杨宏伟.药物分析杂志),2012,(6):1085.
[60] Shim Y S,Yoon W J,Ha J,et al.Food Science & Biotechnology,2013,22(6):1567.
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