汤旺河国家公园红松林土壤真菌多样性研究
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摘要
为了解汤旺河国家公园不同林型的土壤真菌群落结构特征,探讨土壤真菌群落结构变化的影响因子,进一步明确土壤真菌群落结构与土壤环境因子之间的关系和作用。以白桦次生林、阔叶红松林和云冷杉红松林的森林土壤为研究对象,采用高通量测序技术测定0～20 cm土壤层真菌的群落组成和多样性。结果表明,土壤养分中的土壤有机碳、全氮、碱解氮、有效磷和速效钾含量从阔叶林到针叶林依次升高,而土壤pH值降低。此次分析土壤真菌包括3门9纲31目64科82属75种。云冷杉红松林和阔叶红松林中Basidiomycota门为主要优势菌门,白桦次生林中优势菌门为Mucoromycota;相对丰度差别较大的菌属包括Russula、Mortierella、Cenococcum、Pyrenochaetopsis、Solicoccozyma、Saitozyma、Humicolopsis、Tylospora菌属。通过冗余分析和相关性分析发现,汤旺河国家公园不同类型森林土壤真菌多样性可能受土壤p H值、全氮、碱解氮、土壤含水率的影响。
The study aims to understand the community structure characteristics of soil fungi from different forest types in Tangwanghe National Park, discuss the factors affected the community structure change of soil fungi, so as to clarify the relationships and functions of community structure of soil fungi and soil environmental factors. We analyzed the community structure and diversity of fungi in 0-20 cm soil layer collected from 3 forest types(brich secondary forest, broad-leaved Korean pine forest and spruce pine red pine forest) by using the high-throughput sequencing technology. The results showed that: the concentration of soil organic carbon, total nitrogen, alkali nitrogen, available phosphorus and available potassium increased from broad-leaved forests to coniferous forests, but the soil pH value decreased; the soil fungi taxon included 3 phyla, 9 classes, 31 orders, 64 families, 82 genera and 75 species; the Basidiomycota was the dominant phylum in the spruce forest and the broad-leaved Korean pine forests; the Mucoromycota was the dominant phylum in the birch secondary forests; the relative genus abundance of Russula, Mortierella, Cenococcum,Pyrenochaetopsis, Solicoccozyma, Saitozyma, Humicolopsis and Tylospora differed significantly. Based on the redundancy analysis and correlation analysis, the soil fungi diversity of different forest types in Tangwanghe National Park are affected by soil pH value, total nitrogen, available nitrogen and soil moisture.
The study aims to understand the community structure characteristics of soil fungi from different forest types in Tangwanghe National Park, discuss the factors affected the community structure change of soil fungi, so as to clarify the relationships and functions of community structure of soil fungi and soil environmental factors. We analyzed the community structure and diversity of fungi in 0-20 cm soil layer collected from 3 forest types(brich secondary forest, broad-leaved Korean pine forest and spruce pine red pine forest) by using the high-throughput sequencing technology. The results showed that: the concentration of soil organic carbon, total nitrogen, alkali nitrogen, available phosphorus and available potassium increased from broad-leaved forests to coniferous forests, but the soil pH value decreased; the soil fungi taxon included 3 phyla, 9 classes, 31 orders, 64 families, 82 genera and 75 species; the Basidiomycota was the dominant phylum in the spruce forest and the broad-leaved Korean pine forests; the Mucoromycota was the dominant phylum in the birch secondary forests; the relative genus abundance of Russula, Mortierella, Cenococcum,Pyrenochaetopsis, Solicoccozyma, Saitozyma, Humicolopsis and Tylospora differed significantly. Based on the redundancy analysis and correlation analysis, the soil fungi diversity of different forest types in Tangwanghe National Park are affected by soil pH value, total nitrogen, available nitrogen and soil moisture.
引文
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[12]徐飞,蔡体久,杨雪,等.三江平原沼泽湿地垦殖及自然恢复对土壤细菌群落多样性的影响[J].生态学报,2016,36(22):7412-7421.
[13]曲国辉,郭继勋.松嫩平原不同演替阶段植物群落和土壤特性的关系[J].草业学报,2003,12(1):18-22.
[14]岳琳艳,郑俊强,韩士杰,等.长白山温带森林不同演替阶段土壤化学性质及微生物群落结构的变化[J].生态学杂志,2015,34(9):2590-2597.
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[16] Wang W, Wang H, Zu Y. Temporal changes in SOM, N, P, K, and their stoichiometric ratios during reforestation in China and interactions with soil depths:Importance of deep-layer soil and management implications[J]. Forest Ecology&Management,2014,325(325):8-17.
[17]林德喜,樊后保,苏兵强,等.马尾松林下套种阔叶树土壤理化性质的研究[J].土壤学报,2004(4):655-659.
[18]宋洪涛,张劲峰,田昆,等.滇西北亚高山地区黄背栎林植被演替过程中的林地土壤化学响应[J].西部林业科学,2007,36(2):65-70.
[19]丁秋祎,白军红,高海峰,等.黄河三角洲湿地不同植被群落下土壤养分含量特征[J].农业环境科学学报,2009,28(10):2092-2097.
[20]杨菁,周国英,田媛媛,等.降香黄檀不同混交林土壤细菌多样性差异分析[J].生态学报,2015,35(24):8117-8127.
[21] Nyb N, Achouak W, Christen R, et al. Characteristics of microbial habitats in a tropical soil subject to different fallow management[J].Applied Soil Ecology,2008,38(1):51-61.
[22]隋心,张荣涛,许楠,等.三江平原不同退化阶段小叶章湿地土壤真菌群落结构组成变化[J].环境科学,2006,37(9),3598-3605.
[23]隋心,张荣涛,杨立宾,等.三江平原不同类型小叶章湿地土壤细菌群落功能多样性[J].环境科学研究,2016,29(10):1479-1486.
[24] Roxane A, Laurent G, Markusn T, et al. Changes in microbial community structure and function following Sphagnum peatland restoration[J]. Soil Biology&Biochemistry,2010,42(2):291-301.
[25] Tscherko D, Hammesfahr U, Zeltner G, et al. Plant succession and rhizosphere microbial communities in a recently deglaciated alpine terrain[J]. Basic&Applied Ecology,2005,6(4):367-383.
[26]王薇,图力古尔.长白山地区大型真菌的区系组成及生态分布[J].吉林农业大学学报,2015,37(1):26-36.
[2]贾姗姗.汤旺河国家公园的旅游发展实证研究[D].合肥:安徽大学,2013:1-3.
[3]李学成,杨继承.汤旺河首个国家公园建设的意义及管理模式浅析[J].内蒙古林业,2009(10):24-25.
[4]孙雪,隋心,韩冬雪,等.原始红松林退化演替后土壤微生物功能多样性的变化[J].环境科学研究,2017,30(6):911-919.
[5]张玲,张东来,毛子军,等.小兴安岭阔叶红松林不同演替系列土壤有机碳及各组分特征[J].林业科学,2017,53(9):11-17.
[6]郝敬梅,张韫,崔晓阳,等.原始阔叶红松林次生林土壤矿质氮特征及树种吸收反应[J].北京林业大学学报,2014,36(1):21-25.
[7]胡嵩,张颖,史荣久,等.长白山原始红松林次生演替过程中土壤微生物生物量和酶活性变化[J].应用生态学报,2013,24(2):366-372.
[8]周国英,陈小艳,李倩茹,等.油茶林土壤微生物生态分布及土壤酶活性的研究[J].经济林研究,2001,19(1):9-12.
[9]黄志宏,田大伦,梁瑞友,等.南岭不同林型土壤微生物数量特征分析[J].中南林业科技大学学报,2007,27(3):1-13.
[10]吴愉萍.基于磷脂脂肪酸PLFA分析技术的土壤微生物群落结构多样性的研究[D].杭州:浙江大学,2009.
[11]杨立宾,隋心,朱道光,等.大兴安岭兴安落叶松林土壤真菌群落特征研究[J].中南林业科技大学学报,2017,37(12):76-84.
[12]徐飞,蔡体久,杨雪,等.三江平原沼泽湿地垦殖及自然恢复对土壤细菌群落多样性的影响[J].生态学报,2016,36(22):7412-7421.
[13]曲国辉,郭继勋.松嫩平原不同演替阶段植物群落和土壤特性的关系[J].草业学报,2003,12(1):18-22.
[14]岳琳艳,郑俊强,韩士杰,等.长白山温带森林不同演替阶段土壤化学性质及微生物群落结构的变化[J].生态学杂志,2015,34(9):2590-2597.
[15]汲常萍,王文杰韩士杰,等.东北次生杨桦林土壤碳氮动态特征[J].生态学报,2015,35(17):5675-5685.
[16] Wang W, Wang H, Zu Y. Temporal changes in SOM, N, P, K, and their stoichiometric ratios during reforestation in China and interactions with soil depths:Importance of deep-layer soil and management implications[J]. Forest Ecology&Management,2014,325(325):8-17.
[17]林德喜,樊后保,苏兵强,等.马尾松林下套种阔叶树土壤理化性质的研究[J].土壤学报,2004(4):655-659.
[18]宋洪涛,张劲峰,田昆,等.滇西北亚高山地区黄背栎林植被演替过程中的林地土壤化学响应[J].西部林业科学,2007,36(2):65-70.
[19]丁秋祎,白军红,高海峰,等.黄河三角洲湿地不同植被群落下土壤养分含量特征[J].农业环境科学学报,2009,28(10):2092-2097.
[20]杨菁,周国英,田媛媛,等.降香黄檀不同混交林土壤细菌多样性差异分析[J].生态学报,2015,35(24):8117-8127.
[21] Nyb N, Achouak W, Christen R, et al. Characteristics of microbial habitats in a tropical soil subject to different fallow management[J].Applied Soil Ecology,2008,38(1):51-61.
[22]隋心,张荣涛,许楠,等.三江平原不同退化阶段小叶章湿地土壤真菌群落结构组成变化[J].环境科学,2006,37(9),3598-3605.
[23]隋心,张荣涛,杨立宾,等.三江平原不同类型小叶章湿地土壤细菌群落功能多样性[J].环境科学研究,2016,29(10):1479-1486.
[24] Roxane A, Laurent G, Markusn T, et al. Changes in microbial community structure and function following Sphagnum peatland restoration[J]. Soil Biology&Biochemistry,2010,42(2):291-301.
[25] Tscherko D, Hammesfahr U, Zeltner G, et al. Plant succession and rhizosphere microbial communities in a recently deglaciated alpine terrain[J]. Basic&Applied Ecology,2005,6(4):367-383.
[26]王薇,图力古尔.长白山地区大型真菌的区系组成及生态分布[J].吉林农业大学学报,2015,37(1):26-36.
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