外源尿黑酸对拟南芥幼苗花青素生物合成的影响

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英文篇名：Effect of exogenous homogentisate on anthocyanin biosynthesis in Arabidopsis thaliana seedlings 作者：汤睿 ; 雷雨婷 ; 任春梅 英文作者：TANG Rui;LEI Yuting;REN Chunmei;College of Bioscience and Biotechnology,Hunan Agricultural University;Crop Gene Engineering Key Laboratory of Hunan Province; 关键词：拟南芥 ; sscd1突变体 ; 花青素 ; 尿黑酸 ; 酪氨酸降解途径 英文关键词：Arabidopsis;;sscd1 mutant;;anthocyanin;;homogentisate;;tyrosine degradation pathway 中文刊名：湖南农业大学学报(自然科学版) 英文刊名：Journal of Hunan Agricultural University(Natural Sciences) 机构：湖南农业大学生物科学技术学院;作物基因工程湖南省重点实验室; 出版日期：2019-01-30 出版单位：湖南农业大学学报(自然科学版) 年：2019 期：01 基金：国家“973”计划前期研究专项(2014CB160308) 语种：中文; 页：39-43 页数：5 CN：43-1257/S ISSN：1007-1032 分类号：Q946

摘要

以拟南芥Col–0(CK)和酪氨酸降解最后一步缺陷突变体sscd1为试验材料,采用外源尿黑酸处理幼苗,研究尿黑酸对花青素生物合成的影响。结果显示:外源尿黑酸能诱导拟南芥幼苗花青素的积累,而且在sscd1突变体中花青素的积累效果更显著;荧光定量PCR分析发现,尿黑酸处理能促进花青素生物合成基因(包括花青素生物合成的下游基因DFR、LDOX、UF3GT和上游基因PAL、CHI、CHS)的表达,且在sscd1突变体中,这些基因表达上调的幅度更大。以上结果表明,尿黑酸处理能激活花青素生物合成途径,从而促进花青素的积累;酪氨酸降解最后一步缺陷能促进尿黑酸诱导花青素的生物合成。
        In this study, we used Arabidopsis model plant of wild-type and tyrosine pathway-deficient mutant sscd1 in the last step as the experimental materials, analyzed the effect of homogentisate on the synthesis of anthocyanin with the treatment of exogenous homogentisate. The results showed that the exogenous homogentisate could induce anthocyanin accumulation, the accumulation of mutant sscd1 was more significant. Furthermore, the expression of anthocyanin synthesis was analyzed by qRT-PCR. The results showed that homogentisate could promote the expression of upstream genes PAL, CHI, CHS and downstream genes DFR, LDOX, UF3 GT of anthocyanin biosynthesis, similarly, the gene expression insscd1 mutants was up-regulated more. The above results indicated that: the treatment of homogentisate activated the anthocyanin biosynthetic pathway and lead to anthocyanin accumulation. Defects in the final step of the tyrosine degradation pathway could increase the biosynthesis ofanthocyanin which induced by homogentisate.

引文

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