H_2S调节小麦干旱胁迫依赖于钙信号途径
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摘要
探讨了Ca~(2+)和H_2S调节小麦干旱胁迫的交互关系。结果表明,H_2S和Ca~(2+)均可缓解干旱胁迫诱导的氧化伤害,与干旱处理组相比,H_2S和Ca~(2+)预处理显著降低了叶片中丙二醛(MDA)和H_2O_2含量,提高了超氧化物歧化酶(SOD)活性,减少了干旱胁迫对过氧化物酶(POD)和过氧化氢酶(CAT)活性的诱导,但乙二醇双(2-氨基乙基醚)四乙酸(EGTA,钙螯合剂)处理显著削弱了H_2S对干旱胁迫的缓解作用,而亚牛磺酸(HT,H_2S清除剂)处理对干旱胁迫下Ca~(2+)的缓解作用没有影响。此外,干旱胁迫下,H_2S可诱导Ca~(2+)信号途径相关基因表达(如CcaMK,CPK10,CIPK2和CIPK15),而Ca~(2+)对小麦叶片内源H_2S含量无显著影响。由此推测,Ca~(2+)可能位于H_2S信号的下游,部分介导了H_2S对小麦干旱胁迫的调节作用,H_2S可通过促进Ca~(2+)信号途径相关基因的表达调节干旱胁迫。
The interaction between calciumion(Ca~(2+)) and hydrogen sulfide(H_2S) in regulation drought stress for wheat was investigated in this paper. The results showed that both H_2S and Ca~(2+) could relieve the drought-induced oxidative damage. Compared with the drought-treated group, H_2S and Ca~(2+) pretreatment significantly reduced the content of MDA and H_2O_2 in the leaves, increased SOD enzyme activity and decreased drought-inducted POD and CAT activities. However, the treatment of EGTA(calcium chelator) significantly attenuated the alleviating effect of H_2S on drought stress, while, HT(H_2S scavenger) treatment did not affect the regulation of Ca~(2+) on drought stress. In addition, under drought stress, H_2S could induce the expression of Ca~(2+) signaling pathway-related genes(such as CcaMK, CPK10, CIPK2 and CIPK15) under drought stress, but Ca~(2+) had no significant effect on the production of endogenous H_2S in wheat leaves. These results showed that Ca~(2+) may be located downstream of the H_2S signal, partially mediates drought stress regulation by H_2S, H_2S may regulate drought stress by promoting the expression of Ca~(2+) signaling related genes.
The interaction between calciumion(Ca~(2+)) and hydrogen sulfide(H_2S) in regulation drought stress for wheat was investigated in this paper. The results showed that both H_2S and Ca~(2+) could relieve the drought-induced oxidative damage. Compared with the drought-treated group, H_2S and Ca~(2+) pretreatment significantly reduced the content of MDA and H_2O_2 in the leaves, increased SOD enzyme activity and decreased drought-inducted POD and CAT activities. However, the treatment of EGTA(calcium chelator) significantly attenuated the alleviating effect of H_2S on drought stress, while, HT(H_2S scavenger) treatment did not affect the regulation of Ca~(2+) on drought stress. In addition, under drought stress, H_2S could induce the expression of Ca~(2+) signaling pathway-related genes(such as CcaMK, CPK10, CIPK2 and CIPK15) under drought stress, but Ca~(2+) had no significant effect on the production of endogenous H_2S in wheat leaves. These results showed that Ca~(2+) may be located downstream of the H_2S signal, partially mediates drought stress regulation by H_2S, H_2S may regulate drought stress by promoting the expression of Ca~(2+) signaling related genes.
引文
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[21] 方慧慧,裴雁曦,田保华,等.H2S与Ca2+协同增强谷子对Cr6+胁迫的耐受 [J].中国细胞生物学学报,2014,36(6):758-765.
[22] FANG H,JING T,LIU Z,et al.Hydrogen sulfide inte-racts with calcium signaling to enhance the chromium tolerance in Setaria italica[J].Cell Calcium,2014,56(6):472-481.
[23] ZHANG H,JIAO H,JIANG C X,et al.Hydrogen sulfide protects soybean seedlings against drought-induced oxidative stress [J].Acta Physiologiae Plantarum,2010,32(5):849-857.
[24] JIANG M,ZHANG J.Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves[J].Journal of Experimental Botany [J],2002,53(379):2401-2410.
[25] LI H,XU H,ZHANG P,et al.High temperature effects on D1 protein turnover in three wheat varieties with di-fferent heat susceptibility[J].Plant Growth Regulation,2017,81(1):1-9.
[26] FANG T,CAO Z Y,LI J A,et al.Auxin-induced hydrogen sulfide generation is involved in lateral root formation in tomato [J].Plant Physiology and Biochemistry,2014,76:44-51.
[27] KOLUPAEV Y E,FIRSOVA E N,YASTREB T O,et al.The participation of calcium ions and reactive oxygen species in the induction of antioxidant enzymes and heat resistance in plant cells by hydrogen sulfide donor[J].Applied Biochemistry & Microbiology,2017,53(5):573-579.
[2] HUA Z,YE Y K,WANG S H,et al.Hydrogen sulfide counteracts chlorophyll loss in sweetpotato seedling leaves and alleviates oxidative damage against osmotic stress [J].Plant Growth Regulation,2009,58(3):243-250.
[3] ZHANG H,HU L Y,LI P,et al.Hydrogen sulfide alleviated chromium toxicity in wheat[J].Biologia Plantarum,2010,54(4):743-747.
[4] ZHANG H,JIAO H,JIANG C X,et al.Hydrogen sulfide protects soybean seedlings against drought-induced oxidative stress [J].Acta Physiologiae Plantarum,2010,32(5):849-857.
[5] JONES R L.Hydrogen sulfide alleviates aluminum toxi-city in germinating wheat seedlings [J].Chinese Bulletin of Botany ,2010,52(6):556-67.
[6] LI Z G,GONG M,XIE H,et al.Hydrogen sulfide donor sodium hydrosulfide-induced heat tolerance in tobacco (Nicotiana tabacum L.) suspension cultured cells and involvement of Ca2+ and calmodulin [J].Plant Science,2012,185/186(4):185-189.
[7] JIN Z,SHEN J J,QIAO Z J,et al.Hydrogen sulfide improves drought resistance in Arabidopsis thaliana[J].Biochemical and Biophysical Research Communications,2011,414(3):481-486.
[8] CHRISTOU A,MANGANARIS G A,PAPADOPOULOS I,et al.Hydrogen sulfide induces systemic tolerance to salinity and non-ionic osmotic stress in strawberry plants through modification of reactive species biosynthesis and transcriptional regulation of multiple defence pathways [J].Journal of Experimental Botany,2013,64(7):1953-1966.
[9] HUA L,MING Q G,RUI L X,et al.Effect of hydrogen sulfide on D1 protein in wheat under drought stress [J].Acta Physiologiae Plantarum,2015,37(11):225.
[10] 王兰香,侯智慧,侯丽霞,等.H2O2介导的H2S产生参与干旱诱导的拟南芥气孔关闭 [J].植物学报,2012,47(3):217-225.
[11] 郑仲仲,沈金秋,潘伟槐,等.植物钙感受器及其介导的逆境信号途径 [J].遗传,2013,35(7):875-884.
[12] KNIGHT H,TREWAVAS A J,KNIGHT M R.Calcium signalling in Arabidopsis thaliana responding to drought and salinity [J].Plant Journal for Cell & Molecular Biology,1998,12(5):1067-1078.
[13] SHAO H B,CHU L Y,SHAO M A.Calcium as a versatile plant signal transducer under soil water stress [J].Bioessays,2010,30(7):634-641.
[14] SHAO H B,SONG W Y,CHU L Y.Advances of calcium signals involved in plant anti-drought [J].Comptes Rendus-Biologies,2008,331(8):587-596.
[15] NAYYAR H.Variation in osmoregulation in differentially drought-sensitive wheat genotypes involves calcium [J].Biologia Plantarum,2003,47(4):541-547.
[16] XU C,LI X,ZHANG L.The effect of calcium chloride on growth,photosynthesis,and antioxidant responses of Zoysia japonica under drought conditions [J].Plos One,2013,8(7):e68214.
[17] JIN Z,XUE S,LUO Y,ET AL.Hydrogen sulfide interacting with abscisic acid in stomatal regulation responses to drought stress in Arabidopsis[J].Plant Physiology & Biochemistry,2013,62(1):41-46.
[18] GARCíAMATA C,LAMATTINA L.Hydrogen sulphide,a novel gasotransmitter involved in guard cell signalling [J].New Phytologist,2010,188(4):977-984.
[19] SHEN J,XING T,YUAN H,et al.Hydrogen sulfide improves drought tolerance in Arabidopsis thaliana by microRNA expressions [J].Plos One,2013,8(10):e77047.
[20] SHI H,YE T,HAN N,et al.Hydrogen sulfide regulates abiotic stress tolerance and biotic stress resistance in Arabidopsis[J].Chinese Bulletin of Botany,2015,57(7):628-640.
[21] 方慧慧,裴雁曦,田保华,等.H2S与Ca2+协同增强谷子对Cr6+胁迫的耐受 [J].中国细胞生物学学报,2014,36(6):758-765.
[22] FANG H,JING T,LIU Z,et al.Hydrogen sulfide inte-racts with calcium signaling to enhance the chromium tolerance in Setaria italica[J].Cell Calcium,2014,56(6):472-481.
[23] ZHANG H,JIAO H,JIANG C X,et al.Hydrogen sulfide protects soybean seedlings against drought-induced oxidative stress [J].Acta Physiologiae Plantarum,2010,32(5):849-857.
[24] JIANG M,ZHANG J.Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves[J].Journal of Experimental Botany [J],2002,53(379):2401-2410.
[25] LI H,XU H,ZHANG P,et al.High temperature effects on D1 protein turnover in three wheat varieties with di-fferent heat susceptibility[J].Plant Growth Regulation,2017,81(1):1-9.
[26] FANG T,CAO Z Y,LI J A,et al.Auxin-induced hydrogen sulfide generation is involved in lateral root formation in tomato [J].Plant Physiology and Biochemistry,2014,76:44-51.
[27] KOLUPAEV Y E,FIRSOVA E N,YASTREB T O,et al.The participation of calcium ions and reactive oxygen species in the induction of antioxidant enzymes and heat resistance in plant cells by hydrogen sulfide donor[J].Applied Biochemistry & Microbiology,2017,53(5):573-579.
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