外源褪黑素对高温胁迫下厚皮甜瓜幼苗光合及抗氧化特性的影响
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摘要
以厚皮甜瓜‘迎春’杂交种为试材,采用营养钵种植法,设5种不同浓度(50、100、150、200和250μmol/L)的褪黑素(MT)溶液,以清水为对照,经MT溶液处理后对幼苗进行高温胁迫(昼43±1℃/夜32±1℃)4d。通过测定幼苗叶片光合参数及SOD、POD和CAT等生理生化指标,研究外源MT溶液对高温胁迫下幼苗叶片光合作用及抗氧化特性的影响。结果显示:高温胁迫下喷施不同浓度MT溶液,可不同程度地提高厚皮甜瓜幼苗叶片中光合色素含量、光合能力及抗氧化酶活性,与对照相比,200μmol/LMT处理时,厚皮甜瓜幼苗叶片中叶绿素含量和净光合速率达到最高峰,分别为4.43、8.87μmol/(m~2·s);SOD、POD和CAT活性也达到最大,分别为211.28、24.77和376.92U/g;且MDA含量降到最低,为7.93μmol/g。结果表明,高温胁迫下外源MT能增强厚皮甜瓜幼苗抵抗力,且浓度为200μmol/L时效果最佳。
The hybrid of muskmelon‘Yingchun'was used as the test material,and planted in the nutritious pots for the research.Five different concentrations(50,100,150,200 and 250μmol/L)were set up to prepare the melatonin(MT)solution.With clear water as the control,the seedlings were treated with MT solution for 4 days under high temperature stress(day 43±1℃/night 32±1℃).The effects of exogenous MT solution on photosynthesis and antioxidant properties of seedling leaves under high temperature stress were studied by measuring photosynthesis parameters and physiological and biochemical indices such as SOD,POD and CAT.The results showed that spraying different concentrations of MT solution under high temperature stress could increase photosynthetic pigment content,photosynthetic capacity and antioxidant enzyme activity in leaves of muskmelon seedlings in different degrees.Compared with the control,when treated with 200μmol/L MT,The chlorophyll content and net photosynthetic rate of muskmelon seedlings reached the highest peaks,which were 4.43 mg/gand 8.87μmol/(m~2·s),respectively.The activities of SOD,POD and CAT also reached the maximum,which were 211.28,24.77,and 376.92 U/g respectively;and the MDA content was minimized to 7.93μmol/g.The results showed that exogenous MT could enhance the resistance of muskmelon seedlings under high temperature stress,and the best effect was at the concentration of 200μmol/L.
The hybrid of muskmelon‘Yingchun'was used as the test material,and planted in the nutritious pots for the research.Five different concentrations(50,100,150,200 and 250μmol/L)were set up to prepare the melatonin(MT)solution.With clear water as the control,the seedlings were treated with MT solution for 4 days under high temperature stress(day 43±1℃/night 32±1℃).The effects of exogenous MT solution on photosynthesis and antioxidant properties of seedling leaves under high temperature stress were studied by measuring photosynthesis parameters and physiological and biochemical indices such as SOD,POD and CAT.The results showed that spraying different concentrations of MT solution under high temperature stress could increase photosynthetic pigment content,photosynthetic capacity and antioxidant enzyme activity in leaves of muskmelon seedlings in different degrees.Compared with the control,when treated with 200μmol/L MT,The chlorophyll content and net photosynthetic rate of muskmelon seedlings reached the highest peaks,which were 4.43 mg/gand 8.87μmol/(m~2·s),respectively.The activities of SOD,POD and CAT also reached the maximum,which were 211.28,24.77,and 376.92 U/g respectively;and the MDA content was minimized to 7.93μmol/g.The results showed that exogenous MT could enhance the resistance of muskmelon seedlings under high temperature stress,and the best effect was at the concentration of 200μmol/L.
引文
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[14]高青海,王亚坤,陆晓民,等.低温弱光下外源褪黑素对黄瓜幼苗生长及抗氧化系统的影响[J].西北植物学报,2014,34(8):1608-1613.
[15]刘建龙.外源褪黑素对干旱胁迫下番茄抗氧化系统及产量和果实品质的影响[D].杨凌:西北农林科技大学,2015.
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[17]赵世杰,刘华山,董新纯,等.植物生理学实验指导[M].北京:中国农业科技出版,1998:146-148,156-157.
[18]Tewan A K,Tripathy B C.Temperature-stress-induced impaired of chlorophyll biosynthetic reactions in cucumber and wheat[J].Plant Physiol,1998,117(3):851-858.
[19]郭培国,李荣华.夜间高温胁迫对水稻叶片光合作用的影响[J].植物学公报,2000,42(7):673-678.
[20]徐向东,孙艳,郭晓琴,等.外源褪黑素对高温胁迫下黄瓜幼苗光合作用和叶绿素荧光参数的影响[J].核农业科学杂志,2011,25(1):179-184.
[21]邓沛怡,周杰良,等.干旱胁迫对6种藤本植物光合作用及叶绿素荧光参数的影响[J].南农业大学学报:自然科学版,2015,41(3):263-270.
[22]齐红岩,王丹,齐明芳,等.高温胁迫下不同形式钙对番茄叶片光合作用的影响[J].应用生态学报,2014(12):3540-3546.
[23]徐向东.外源褪黑素对高温胁迫下黄瓜幼苗生理反应的影响[D].杨凌:西北农林科技大学,2010.
[24]沈素贞,聂东伶,吴思政,等.高温胁迫对广东万年青生理特性的影响[J].湖南农业大学学报:自然科学版,2014,40(1):28-31.
[25]Li C,Wei Z W.The mitigation effects of exogenous melatonin on salinity-induced stress in Malushupehensis[J].Journal of Pineal Research,2012,53(3):298-306.
[26]曾庆栋,许忠民,张恩慧,等.外源褪黑素对高温胁迫下甘蓝幼苗生理特性的影响[J].北方园艺,2017(20):12-17.
[2]左彬彬,张彦萍,刘海河,等.茉莉酸甲酯对厚皮甜瓜幼苗耐冷性的影响[J].河北农业大学学报,2018,41(5):75-79.
[3]杜天浩.外源褪黑素对NaCl胁迫下番茄幼苗生理代谢和产量及品质的影响[D].杨凌:西北农林科技大学,2016.
[4]张永平,杨少军,陈幼源,等.2,4-油菜素内酯对高温胁迫下甜瓜幼苗抗氧化酶活性和光合作用的影响[J].西北植物学报,2011,31(07):1347-1354.
[5]张永平,陈幼源,杨少军.高温胁迫对不同甜瓜品种幼苗生长和生理特性的影响[J].上海农业学报,2011,27(2):71-76.
[6]杨秋珍,李军,王金霞,等.高温胁迫下甜瓜生理生态特性研究[J].中国生态农业学报,2003(1):26-28.
[7]左佳琦,谢佳恒,薛宇轩,等.褪黑素缓解植物胁迫的研究进展[J].基因组学与应用生物学,2014,23(3):709-715.
[8]Reiter R J.Oxidative damage in the central nervous system:protection by melatonin[J].Progress in Neurobiology,2006,56(3):359-384.
[9]Arnao M B,Hernández-Ruiz J.Growth conditions influence the melatonin content of tomato plants[J].Food Chemistry,2013,138(2/3):1212-1214.
[10]Dubbels R,Reiter R J,Klenke E,et al.Melatonin in edible plants identified by radio immunoassay and by high performance liquid chromatography-mass spectrometry[J].Journal of Pineal Resewrch,1995,18(1):28-31.
[11]Janas K M,Posmyk M M.Melatonin,an understimated natural substance with great potential for agricultural application[J].Acta Physiologiae Plantarum,2013,35(12):3285-3292.
[12]Li C,Wang P,Wwei Z W,et al.The mitigation effects of exogenous melatonin on salinity-iinduced stress in Malus hupehensis[J].Journal of Pineal Resarch,2012,53(3):298-306.
[13]Posmyk M M,Kuran H,Marciniak K,et al.Presowing seed treatment with melatonin protects red cabbafe seedlings against toxic copper ion concentrations[J].Journal of Pineal Research,2008,45(1):24-31.
[14]高青海,王亚坤,陆晓民,等.低温弱光下外源褪黑素对黄瓜幼苗生长及抗氧化系统的影响[J].西北植物学报,2014,34(8):1608-1613.
[15]刘建龙.外源褪黑素对干旱胁迫下番茄抗氧化系统及产量和果实品质的影响[D].杨凌:西北农林科技大学,2015.
[16]李合生.植物生理生化实验原理与技术[M].北京:高等教育出版社,2001.
[17]赵世杰,刘华山,董新纯,等.植物生理学实验指导[M].北京:中国农业科技出版,1998:146-148,156-157.
[18]Tewan A K,Tripathy B C.Temperature-stress-induced impaired of chlorophyll biosynthetic reactions in cucumber and wheat[J].Plant Physiol,1998,117(3):851-858.
[19]郭培国,李荣华.夜间高温胁迫对水稻叶片光合作用的影响[J].植物学公报,2000,42(7):673-678.
[20]徐向东,孙艳,郭晓琴,等.外源褪黑素对高温胁迫下黄瓜幼苗光合作用和叶绿素荧光参数的影响[J].核农业科学杂志,2011,25(1):179-184.
[21]邓沛怡,周杰良,等.干旱胁迫对6种藤本植物光合作用及叶绿素荧光参数的影响[J].南农业大学学报:自然科学版,2015,41(3):263-270.
[22]齐红岩,王丹,齐明芳,等.高温胁迫下不同形式钙对番茄叶片光合作用的影响[J].应用生态学报,2014(12):3540-3546.
[23]徐向东.外源褪黑素对高温胁迫下黄瓜幼苗生理反应的影响[D].杨凌:西北农林科技大学,2010.
[24]沈素贞,聂东伶,吴思政,等.高温胁迫对广东万年青生理特性的影响[J].湖南农业大学学报:自然科学版,2014,40(1):28-31.
[25]Li C,Wei Z W.The mitigation effects of exogenous melatonin on salinity-induced stress in Malushupehensis[J].Journal of Pineal Research,2012,53(3):298-306.
[26]曾庆栋,许忠民,张恩慧,等.外源褪黑素对高温胁迫下甘蓝幼苗生理特性的影响[J].北方园艺,2017(20):12-17.
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