采前高浓度赤霉素处理对“贵妃”杧果实色泽发育的抑制效应
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摘要
以10年生"贵妃"杧植株为材料,设置赤霉素(GA_3)0、0.5、1.0、2.0和3.0 g/L等处理,分别于盛花期、幼果期和果实膨大期进行喷洒,比较不同处理对采后杧果果实色泽变化的影响。结果表明,与对照相比,不同浓度GA_3处理对果皮色度a~*值均具有明显影响,浓度越高,抑制a~*值增大的效果越显著;GA_3处理可以显著抑制果皮叶绿素的降解,降低类胡萝卜素及花青素的合成;高浓度GA_3对叶绿素酶、查尔酮异构酶活性增加、5-氨基乙酰脱氢酶活性的降低具有明显的抑制效果。说明采前GA_3处理可以抑制采后"贵妃"杧果皮色泽转变,浓度越高对果皮转色的抑制效果越显著。
引文
[1]黄铭慧,冯舒涵,冯颜,等.采前喷洒赤霉素对‘红贵妃’杧果贮藏品质和采后生理的影响[J].食品科学,2015,36(10):239-244
[2]董绪.黄瓜嫩果皮白色基因的精细定位及果实硬度的遗传研究[D].武汉:华中农业大学,2015
[3]文良娟,刘昊,王维.不同品种杧果总黄酮的抗氧化活性[J].食品科学,2011,32(19):79-82
[4]杨杨,范蓓,申琳,等.杧果采后冷害发生及控制技术研究进展[J].食品科学,2014,35(7):292-297
[5]黄桃鹏,李媚娟,王睿,等.赤霉素生物合成及信号转导途径研究进展[J].植物生理学报,2015,51(8):1241-1247
[6]黄森,张继澍,张院民.赤霉素处理对采后柿果实乙烯生物合成的影响[J].中国农学通报,2006,22(2):88-90
[7]武红霞,许文天,罗纯,等.Irwin杧果果皮着色与相关酶活性的关系[J].中国南方果树,2016,45(6):61-64
[8]MEDLICOTT A P,SIGRIST J M M,SY O.Ripening of mangoes following low-temperature storage[J].Journal of the American Society for Horticultural Science,1990,115(3):430-434
[9]张波,赵志常,高爱平,等.杧果二氢黄酮醇4-还原酶(DFR)基因的克隆及其表达分析[J].分子植物育种,2015,13(4):816-821
[10]FADEEL A A.Location and properties of chloroplasts and pigment determination in roots[J].Physiologia Plantarum,2006,15(1):130-146
[11]周凤丽.银杏叶绿素降解酶的诱导表达研究[D].无锡:江南大学,2008
[12]MAUZERALL D,GRANICK S.The occurrence and determination of 5-aminolevulinic acid and porphobilinogen in urine[J].Journal of Biological Chemistry,1956,219(1):435-446
[13]王惠聪,黄旭明,胡桂兵,等.荔枝果皮花青苷合成与相关酶的关系研究[J].中国农业科学,2004,37(12):2028-2032
[14]杨清,艾沙江·买买提,王志霞,等.DA-6对桃树叶片叶绿素合成途径的调控研究[J].园艺学报,2012,39(4):621-628
[15]卢晓明.5-氨基酮戊酸脱水酶基因的克隆表达及与大蒜绿变关系的研究[D].泰安:山东农业大学,2013
[16]许璇.δ-氨基酮戊酸脱水酶性质及其与大蒜绿变的关系[D].泰安:山东农业大学,2014
[17]赵婉.海南龙血树查尔酮异构酶基因(DcCHI)的克隆及其启动子功能分析[D].大庆:黑龙江八一农垦大学,2017
[18]LISTER C E,LANCASTER J E,JRL W.Developmental changes in enzymes of flavonoid biosynthesis in the skins of red and green apple cultivars[J].Journal of the Science of Food&Agriculture,2015,71(3):313-320
[19]曾凤,张淑娟,李雯.采前喷洒赤霉素对采后“贵妃”杧果外观品质的影响[J].保鲜与加工,2017,17(2):7-11
[20]王延书.葡萄果实着色前后ABA代谢合成关键酶的基因表达分析[D].泰安:山东农业大学,2011
[21]霍珊珊,惠竹梅,马立娜,等.植物生长调节剂对赤霞珠葡萄果实品质的影响[J].西北农林科技大学学报(自然科学版),2012,40(1):183-189
[22]ROKAYA P R,BARAl D R,GAUTAM D M,et al.Effect of pre-harvest application of gibberellic acid on fruit quality and shelf life of mandarin(Citrus reticulata Blanco)[J].American Journal of Plant Sciences,2016,7(7):1033-1039
[23]王贵元,夏仁学.外源ABA和GA3对红肉脐橙果皮色素含量的影响[J].植物生理学通讯,2005,41(2):168-170
[24]任邦来,张灵.不同浓度赤霉素处理对油桃保鲜效果的影响[J].中国食物与营养,2013,19(4):29-32
[25]MCDONALD R E,GREANY P D,SHAW P E,et al.Preharvest applications of gibberellic acid delay senescence of Florida grapefruit[J].Journal of Pomology&Horticultural Science,1997,72(3):461-468
[26]JOSéA H-H,MARíA J F.Degradation kinetics of pigment,colour and stability of the antioxidant capacity in juice model systems from six anthocyanin sources[J].International Journal of Food Science&Technology,2011,46(12):2550-2557
[27]GROSS J,BAZAK H,BLUMENFELD A,et al.Changes in chlorophyll and carotenoid pigments in the peel of‘Triumph’persimmon(Diospyros kaki,L.)induced by pre-harvest gibberellin(GA3)treatment[J].Scientia Horticulturae,1984,24(3):305-314
[28]MARTíNEZ G A,CHAVES A R,ANON M C.Effect of gibberellic acid on ripening of strawberry fruits(Fragaria annanassa,Duch.)[J].Journal of Plant Growth Regulation,1994,13(2):87-91
[29]LISTER C E,LANCASTER J E,WALKER J R L.Phenylalanine ammonia-lyase(PAL)activity and its relationship to anthocyanin and flavonoid levels in New Zealand-grown apple cultivars[J].American Society for Horticultural Science,1996,121(2):281-285
[30]崔艳涛,孟庆瑞,王文凤,等.安哥诺李果皮花青苷与内源激素、酶活性变化规律及其相关性[J].果树学报,2006,23(5):699-702
[31]朱敏,邓穗生,麦贤家,等.GA3和CPPU对海南贵妃杧产量和果实品质的影响[J].热带作物学报,2014,35(9):1784-1790
[2]董绪.黄瓜嫩果皮白色基因的精细定位及果实硬度的遗传研究[D].武汉:华中农业大学,2015
[3]文良娟,刘昊,王维.不同品种杧果总黄酮的抗氧化活性[J].食品科学,2011,32(19):79-82
[4]杨杨,范蓓,申琳,等.杧果采后冷害发生及控制技术研究进展[J].食品科学,2014,35(7):292-297
[5]黄桃鹏,李媚娟,王睿,等.赤霉素生物合成及信号转导途径研究进展[J].植物生理学报,2015,51(8):1241-1247
[6]黄森,张继澍,张院民.赤霉素处理对采后柿果实乙烯生物合成的影响[J].中国农学通报,2006,22(2):88-90
[7]武红霞,许文天,罗纯,等.Irwin杧果果皮着色与相关酶活性的关系[J].中国南方果树,2016,45(6):61-64
[8]MEDLICOTT A P,SIGRIST J M M,SY O.Ripening of mangoes following low-temperature storage[J].Journal of the American Society for Horticultural Science,1990,115(3):430-434
[9]张波,赵志常,高爱平,等.杧果二氢黄酮醇4-还原酶(DFR)基因的克隆及其表达分析[J].分子植物育种,2015,13(4):816-821
[10]FADEEL A A.Location and properties of chloroplasts and pigment determination in roots[J].Physiologia Plantarum,2006,15(1):130-146
[11]周凤丽.银杏叶绿素降解酶的诱导表达研究[D].无锡:江南大学,2008
[12]MAUZERALL D,GRANICK S.The occurrence and determination of 5-aminolevulinic acid and porphobilinogen in urine[J].Journal of Biological Chemistry,1956,219(1):435-446
[13]王惠聪,黄旭明,胡桂兵,等.荔枝果皮花青苷合成与相关酶的关系研究[J].中国农业科学,2004,37(12):2028-2032
[14]杨清,艾沙江·买买提,王志霞,等.DA-6对桃树叶片叶绿素合成途径的调控研究[J].园艺学报,2012,39(4):621-628
[15]卢晓明.5-氨基酮戊酸脱水酶基因的克隆表达及与大蒜绿变关系的研究[D].泰安:山东农业大学,2013
[16]许璇.δ-氨基酮戊酸脱水酶性质及其与大蒜绿变的关系[D].泰安:山东农业大学,2014
[17]赵婉.海南龙血树查尔酮异构酶基因(DcCHI)的克隆及其启动子功能分析[D].大庆:黑龙江八一农垦大学,2017
[18]LISTER C E,LANCASTER J E,JRL W.Developmental changes in enzymes of flavonoid biosynthesis in the skins of red and green apple cultivars[J].Journal of the Science of Food&Agriculture,2015,71(3):313-320
[19]曾凤,张淑娟,李雯.采前喷洒赤霉素对采后“贵妃”杧果外观品质的影响[J].保鲜与加工,2017,17(2):7-11
[20]王延书.葡萄果实着色前后ABA代谢合成关键酶的基因表达分析[D].泰安:山东农业大学,2011
[21]霍珊珊,惠竹梅,马立娜,等.植物生长调节剂对赤霞珠葡萄果实品质的影响[J].西北农林科技大学学报(自然科学版),2012,40(1):183-189
[22]ROKAYA P R,BARAl D R,GAUTAM D M,et al.Effect of pre-harvest application of gibberellic acid on fruit quality and shelf life of mandarin(Citrus reticulata Blanco)[J].American Journal of Plant Sciences,2016,7(7):1033-1039
[23]王贵元,夏仁学.外源ABA和GA3对红肉脐橙果皮色素含量的影响[J].植物生理学通讯,2005,41(2):168-170
[24]任邦来,张灵.不同浓度赤霉素处理对油桃保鲜效果的影响[J].中国食物与营养,2013,19(4):29-32
[25]MCDONALD R E,GREANY P D,SHAW P E,et al.Preharvest applications of gibberellic acid delay senescence of Florida grapefruit[J].Journal of Pomology&Horticultural Science,1997,72(3):461-468
[26]JOSéA H-H,MARíA J F.Degradation kinetics of pigment,colour and stability of the antioxidant capacity in juice model systems from six anthocyanin sources[J].International Journal of Food Science&Technology,2011,46(12):2550-2557
[27]GROSS J,BAZAK H,BLUMENFELD A,et al.Changes in chlorophyll and carotenoid pigments in the peel of‘Triumph’persimmon(Diospyros kaki,L.)induced by pre-harvest gibberellin(GA3)treatment[J].Scientia Horticulturae,1984,24(3):305-314
[28]MARTíNEZ G A,CHAVES A R,ANON M C.Effect of gibberellic acid on ripening of strawberry fruits(Fragaria annanassa,Duch.)[J].Journal of Plant Growth Regulation,1994,13(2):87-91
[29]LISTER C E,LANCASTER J E,WALKER J R L.Phenylalanine ammonia-lyase(PAL)activity and its relationship to anthocyanin and flavonoid levels in New Zealand-grown apple cultivars[J].American Society for Horticultural Science,1996,121(2):281-285
[30]崔艳涛,孟庆瑞,王文凤,等.安哥诺李果皮花青苷与内源激素、酶活性变化规律及其相关性[J].果树学报,2006,23(5):699-702
[31]朱敏,邓穗生,麦贤家,等.GA3和CPPU对海南贵妃杧产量和果实品质的影响[J].热带作物学报,2014,35(9):1784-1790
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