绵羊FSTL3基因g.40674542C>T位点多态性与产羔数的关联分析
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摘要
文章旨在探究绵羊FSTL3基因g.40674542C>T位点多态性与绵羊产羔数之间的关系,以期为绵羊高繁殖力分子育种提供新的遗传标记。利用全基因组重测序结合Sequenom MassARRAY~SNP技术对常年发情绵羊品种(小尾寒羊、策勒黑羊和湖羊)和季节性发情绵羊品种(滩羊、苏尼特羊和草原型藏羊)FSTL3基因g.40674542C>T位点多态性进行了检测,并与小尾寒羊产羔数进行了关联分析。结果表明:FSTL3基因g.40674542C>T位点存在CC、TC和TT三种基因型,基因型频率和等位基因频率在两种发情模式绵羊品种间差异均不显著(P>0.05);g.40674542C>T位点在6个绵羊品种中均表现为低度多态(PIC<0.25),经卡方适合性检验,该位点在小尾寒羊、湖羊和草原型藏羊中处于哈代温伯格平衡状态(P>0.05),在其余3个绵羊品种中处于哈代温伯格不平衡状态;g.40674542C>T位点不同基因型与小尾寒羊第一、第二以及第三胎产羔数均无显著关联(P>0.05)。说明FSTL3基因g.40674542C>T位点不适合用于小尾寒羊产羔数选育。
To elucidate the association between FSTL3 gene polymorphism and litter size in sheep. Year-round estrous sheep breeds(Small-tailed Han sheep,Cele black sheep and Hu sheep)and seasonal estrous sheep breeds(Tan sheep,Sunite sheep and Prairie Tibetan sheep)were selected and Sequenom Mass ARRAY~SNP assay was applied to genotype g.40674542C>T locus of FSTL3 gene. Then the association was analyzed between FSTL3 and litter size in Small-tailed Han sheep. The result of genotyping showed that three genotypes were found at g.40674542C>T locus in both year-round estrous and seasonal estrous sheep,there was no significant difference between year-round estrous and seasonal estrous sheep in both genotype frequency and allele frequency(P >0.05) of g.40674542C>T locus of FSTL3 gene. Population genetic analysis showed that the g.40674542C>T locus was at low polymorphism(PIC<0.25)in all sheep breeds.The χ2 test indicated that the g.40674542C>T locus of Small-tailed Han sheep,Hu sheep and Prairie Tibetan sheep was under Hardy-Weinberg equilibrium(P >0.05). Association analysis indicated that the polymorphism of g.40674542C>T had no correlation with the litter size of the first,second or third parity in Small-tailed Han sheep(P >0.05). Polymorphism of the g.40674542C>T locus of FSTL3 gene had no significant effect on litter size of Small-tailed Han sheep.
To elucidate the association between FSTL3 gene polymorphism and litter size in sheep. Year-round estrous sheep breeds(Small-tailed Han sheep,Cele black sheep and Hu sheep)and seasonal estrous sheep breeds(Tan sheep,Sunite sheep and Prairie Tibetan sheep)were selected and Sequenom Mass ARRAY~SNP assay was applied to genotype g.40674542C>T locus of FSTL3 gene. Then the association was analyzed between FSTL3 and litter size in Small-tailed Han sheep. The result of genotyping showed that three genotypes were found at g.40674542C>T locus in both year-round estrous and seasonal estrous sheep,there was no significant difference between year-round estrous and seasonal estrous sheep in both genotype frequency and allele frequency(P >0.05) of g.40674542C>T locus of FSTL3 gene. Population genetic analysis showed that the g.40674542C>T locus was at low polymorphism(PIC<0.25)in all sheep breeds.The χ2 test indicated that the g.40674542C>T locus of Small-tailed Han sheep,Hu sheep and Prairie Tibetan sheep was under Hardy-Weinberg equilibrium(P >0.05). Association analysis indicated that the polymorphism of g.40674542C>T had no correlation with the litter size of the first,second or third parity in Small-tailed Han sheep(P >0.05). Polymorphism of the g.40674542C>T locus of FSTL3 gene had no significant effect on litter size of Small-tailed Han sheep.
引文
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[14]Oldknow K J,Seebacher J,Goswami T,et al.Follistatin-like 3(FSTL3)mediated silencing of transforming growth factorβ(TGFβ)signaling is essential for testicular aging and regulating testis size[J].Endocrinology,2013,154(3):1310-1320.
[15] Yu L,Hébert M C,Zhang Y E.TGFβreceptor activated p38 MAP kinase mediates Smad independent TGFβresponses[J].EMBO Journal,2014,21(14):3749-3759.
[16] Otsuka F.Modulation of bone morphogenetic protein activity by melatonin in ovarian steroidogenesis[J].Reproductive Medicine&Biology,2018,17(3):228-233.
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[18] Hamidabadi H G,Pasbakhsh P,Amidi F,et al.Functional concentrations of BMP4 on differentiation of mouse embryonic stem cells to primordial germ cells[J].International Journal of Fertility&Sterility,2011,5(2):104.
[19] Tang X,Xu S,Zhang H,et al.Retinoic acid promotes expression of germline-specific genes in chicken blastoderm cells by stimulating Smad1/5 phosphorylation in a feeder-free culture system[J].BMC Biotechnology,2017,17(1):17.
[20] Yu M,Xu Y,Yu D,et al.Comparative analysis of temporal gene expression patterns in the developing ovary of the embryonic chicken[J].J Reprod Dev,2015,61(2):123-133.
[21]Maypanloup P,Ferrél'Hotellier V,Morinière C,et al.Molecular characterization of corona radiata cells from patients with diminished ovarian reserve using microarray and microfluidic-based gene expression profiling[J].Human Reproduction,2012,27(3):829-843.
[22] Nevalainen J,Korpimaki T,Kouru H,et al.Performance of first trimester biochemical markers and mean arterial pressure in prediction of early-onset pre-eclampsia[J].Metabolism-clinical&Experimental,2017,75:6-15.
[23] Kelaini S,Vilàgonzález M,Caines R,et al.FSTL3 enhances the function of endothelial cells derived from pluripotent stem cells by facilitatingβ-catenin nuclear translocation through inhibition of GSK3βactivity[J].Stem Cells,2018,36(7):1033-1044.
[24] Brandt C,Hansen R H,Hansen J B,et al.Over-expression of follistatin-like 3 attenuates fat accumulation and improves insulin sensitivity in mice[J].Metabolism-clinical&Experimental,2015,64(2):283-295.
[25] Xia Y,Sidis Y,Schneyer A.Over-expression of follistatin-like 3 in gonads causes defects in gonadal development and function in transgenic mice[J].Molecular Endocrinology,2004,18(4):979-994.
[26]潘章源,狄冉,刘秋月,等.绵羊多羔主效基因BMPR1B的研究进展[J].家畜生态学报,2015,36(5):1-6.
[27] Moura G R,Pinheiro M,Freitas A,et al.Species-specific codon context rules unveil non-neutrality effects of synonymous mutations[J].PLo S ONE,2011,6(10):e26817.
[28] Rudolph K L M,Schmitt B M,Villar D,et al.Codon-driven translational efficiency is stable across diverse mammalian cell states[J].PLo S Genetics,2016,12(5):e1006024.
[2]吕晓阳,孙伟,张想想,等.利用9个微卫星标记分析海门山羊和徐淮山羊的遗传多样性[J].中国畜牧杂志,2016,52(15):5-11.
[3] Vries F D,Hamann H,Drogemüller C,et al.Analysis of associations between the prion protein genotype and reproduction traits in meat sheep breeds[J].Animal Science,2016,79(3):397-404.
[4] Vázquezarmijo J F,Rojo R,López D,et al.Trace elements in sheep and goats reproduction:A review[J].Tropical&Subtropical Agroecosystems,2016,14(1):1-13.
[5]贺小云,狄冉,胡文萍,等.绵羊季节性繁殖的神经内分泌研究进展[J].畜牧兽医学报,2018,49(1):18-25.
[6] Kralisch S,Hoffmann A,Kloting N,et al.FSTL3 is increased in renal dysfunction[J].Nephrol Dial Transplant,2017,32(10):1637-1644.
[7] Robertson R D,Mukherjee A.Synexpression group analyses identify new functions of FSTL3,a TGFβligand inhibitor[J].Biochemical&Biophysical Research Communications,2012,427(3):568-573.
[8]雷梦媛.FSTL3基因在绵羊常年发情中的功能研究[D].成都:四川农业大学,2015.
[9] An Z,Blockx H,Arnhem W V,et al.Characterization of a rat C6glioma secreted follistatin related protein(FRP)[J].European Journal of Biochemistry,2010,225(3):937-946.
[10] Wang H Q,Takebayashi K,Tsuchida K,et al.Follistatin-related gene(FLRG)expression in human endometrium:sex steroid hormones regulate the expression of FLRG in cultured human endometrial stromal cells[J].Journal of Clinical Endocrinology&Metabolism,2003,88(9):4432.
[11] Florio P,Ciarmela P,Toti P,et al.Human endometrium and decidua express follistatin-related gene(FLRG)m RNA and peptide[J].Molecular&Cellular Endocrinology,2004,218(1):129-135.
[12]周梅,曹晓涵,贺小云,等.绵羊FGF7基因组织表达及其多态性与产羔数之间的关系[J].畜牧兽医学报,2018,49(3):525-533.
[13]Thadhani R,Powe C E,Tjoa M L,et al.First-trimester follistatinlike-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus[J].Diabetes Care,2010,33(3):664-669.
[14]Oldknow K J,Seebacher J,Goswami T,et al.Follistatin-like 3(FSTL3)mediated silencing of transforming growth factorβ(TGFβ)signaling is essential for testicular aging and regulating testis size[J].Endocrinology,2013,154(3):1310-1320.
[15] Yu L,Hébert M C,Zhang Y E.TGFβreceptor activated p38 MAP kinase mediates Smad independent TGFβresponses[J].EMBO Journal,2014,21(14):3749-3759.
[16] Otsuka F.Modulation of bone morphogenetic protein activity by melatonin in ovarian steroidogenesis[J].Reproductive Medicine&Biology,2018,17(3):228-233.
[17]徐业芬.Smad信号转导通路相关基因表达与湖羊高繁殖力关系的研究[D].南京:南京农业大学,2010.
[18] Hamidabadi H G,Pasbakhsh P,Amidi F,et al.Functional concentrations of BMP4 on differentiation of mouse embryonic stem cells to primordial germ cells[J].International Journal of Fertility&Sterility,2011,5(2):104.
[19] Tang X,Xu S,Zhang H,et al.Retinoic acid promotes expression of germline-specific genes in chicken blastoderm cells by stimulating Smad1/5 phosphorylation in a feeder-free culture system[J].BMC Biotechnology,2017,17(1):17.
[20] Yu M,Xu Y,Yu D,et al.Comparative analysis of temporal gene expression patterns in the developing ovary of the embryonic chicken[J].J Reprod Dev,2015,61(2):123-133.
[21]Maypanloup P,Ferrél'Hotellier V,Morinière C,et al.Molecular characterization of corona radiata cells from patients with diminished ovarian reserve using microarray and microfluidic-based gene expression profiling[J].Human Reproduction,2012,27(3):829-843.
[22] Nevalainen J,Korpimaki T,Kouru H,et al.Performance of first trimester biochemical markers and mean arterial pressure in prediction of early-onset pre-eclampsia[J].Metabolism-clinical&Experimental,2017,75:6-15.
[23] Kelaini S,Vilàgonzález M,Caines R,et al.FSTL3 enhances the function of endothelial cells derived from pluripotent stem cells by facilitatingβ-catenin nuclear translocation through inhibition of GSK3βactivity[J].Stem Cells,2018,36(7):1033-1044.
[24] Brandt C,Hansen R H,Hansen J B,et al.Over-expression of follistatin-like 3 attenuates fat accumulation and improves insulin sensitivity in mice[J].Metabolism-clinical&Experimental,2015,64(2):283-295.
[25] Xia Y,Sidis Y,Schneyer A.Over-expression of follistatin-like 3 in gonads causes defects in gonadal development and function in transgenic mice[J].Molecular Endocrinology,2004,18(4):979-994.
[26]潘章源,狄冉,刘秋月,等.绵羊多羔主效基因BMPR1B的研究进展[J].家畜生态学报,2015,36(5):1-6.
[27] Moura G R,Pinheiro M,Freitas A,et al.Species-specific codon context rules unveil non-neutrality effects of synonymous mutations[J].PLo S ONE,2011,6(10):e26817.
[28] Rudolph K L M,Schmitt B M,Villar D,et al.Codon-driven translational efficiency is stable across diverse mammalian cell states[J].PLo S Genetics,2016,12(5):e1006024.
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