微生物菌体包埋固定化技术在废水处理中的应用
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摘要
介绍了污废水生物处理中的微生物菌体包埋固定化技术,总结了该技术的研究现状及其在污水处理领域(脱氮、脱氨、降解有毒物质等)的应用。微生物菌体包埋固定化技术因具有的生物量含量高、包埋微生物类型及污水处理范围广、单位体积微生物菌体或酶活性高、固液分离方便及下游处理操作简单、抗冲击负荷及抵抗搅拌剪切力强等优点,在水处理领域受到广泛研究及关注。但目前研究多集中在包埋材料的制备、包埋菌种的选择等方面,未见将其大规模用于工业应用的报道。在今后的研究中,应对微生物菌体包埋技术的应用领域进行扩增,并可将其与细胞生物学、酶工程、生物催化剂和环境工程等领域进行结合。
The microorganism immobilization technology for wastewater treatment was introduced, the current research status of this technology and its application in wastewater treatment field such as denitrification, deamination and toxic substance degradation were summarized. Due to microorganism immobilization technology have high biomass content, wide range of embedded microorganism types and wastewater treatment, high activity of unit volume microorganism and enzyme, convenient solid-liquid separation, simplicity of downstream processing operation, strong resistance to impact load and mixing shear force, it has received much research and attention. Most of its research still focuses on the preparation of immobilizing materials and the selection of immobilizing bacteria at present, and there is no report about its industrial application. In future studies, the application field of microorganism immobilization technology should be augmented, and it would be combined with cytobiology, enzyme engineering, biocatalyst and environmental engineering.
The microorganism immobilization technology for wastewater treatment was introduced, the current research status of this technology and its application in wastewater treatment field such as denitrification, deamination and toxic substance degradation were summarized. Due to microorganism immobilization technology have high biomass content, wide range of embedded microorganism types and wastewater treatment, high activity of unit volume microorganism and enzyme, convenient solid-liquid separation, simplicity of downstream processing operation, strong resistance to impact load and mixing shear force, it has received much research and attention. Most of its research still focuses on the preparation of immobilizing materials and the selection of immobilizing bacteria at present, and there is no report about its industrial application. In future studies, the application field of microorganism immobilization technology should be augmented, and it would be combined with cytobiology, enzyme engineering, biocatalyst and environmental engineering.
引文
[1]CHO Y H,KNORR D,Development of a gel and foam matrix as immobilization system for cells for microbial denitrification of water[J].Food Biotechnology,1993,7(2):115-126.
[2]NAKANO K,IWASAWA H,ITO O,et al.Improved simultaneous nitrification and denitrification in a single reactor by using two different immobilization carriers with specific oxygen transfer characteristics[J].Bioprocess and Biosystems Engineering,2004,26(3):141-145.
[3]SUN S Y,FAN S L,SHEN K X,et al.Laboratory assessment of bioleaching of shallow eutrophic sediment by immobilized photosynthetic bacteria[J].Environmental Science and Pollution Research2017,24(28):22143-22151.
[4]SUN S Y,FAN S L,SHEN K X,et al.Tannin-immobilized cellulose microspheres as effective adsorbents for removing cationic dye(Methylene Blue)from aqueous solution[J].Journal of Chemical Technology and Biotechnology,2017,92(6):1276-1284.
[5]陈珍妮,唐明阳.一种固定化微生物处理高氨氮及有机废水系统CN106587353A[P].2017-04-26.
[6]HERKOMMEROV魣K,ZEMAN魱KOV魣J,SYCHROV魣H,et al Immobilization in polyvinyl alcohol hydrogel enhances yeast storage stability and reusability of recombinant laccase-producing S-cerevisiae[J].Biotechnology Letters,2018,40(2):405-411.
[7]ZUKI S N S M,Tan L L,AZMI N S,et al.A whole cell bio-optode based on immobilized nitrite-degrading microorganism on the acrylic microspheres for visual quantitation of nitrite ion[J].Sensors and Actuators B-Chemical,2018,255:2844-2852.
[8]RAJESH BANU J,USHANIA U,RAJKUMARB M,et al.Impac of mild alkali dosage on immobilized Exiguobacterium spp.mediated cost and energy efficient sludge disi ntegration[J].Bioresource Technology,2017,245:434-441.
[9]DONG W J,LU G,YAN L,et al.Characteristics of pellets with immobilized activated sludge and its performance in increasing nitrification in sequencing batch reactors at low temperatures[J].Journal of Environmental Sciences,2016,42:202-209.
[10]NISHIDA V S,OLIVEIRA DE R F,BRUGNARI T,et al.Immobilization of Aspergillus awamori beta-glucosidase on commercial gelatin:An inexpensive and efficient process[J].International Journal of Biological Macromolecules,2018,111:1206-1213.
[11]AKPOR O B.Effect of nitrogen source concentration on decolouration rates of laboratory dyes by immobilized cells of two bacterial species[J].World Journal of Microbiology&Biotechnology,2018,34(1):18
[12]LIU Y K,MAO Y,WANG J.Methods and application of immobilized microorganism in PVA[J].Science&Technology Review,2016,34(22):56-61.
[13]ZDARTA J,EDRZAK A J,KLAPISZEWSKI覵,et al.Immobilization of Cellulase on a functional inorganic-organic hybrid support:stability and kinetic study[J].Catalysts,2017,7(12)374.
[14]单晓静,于德爽,李津,等.海水优化ANAMMOX包埋固定化及其处理含海水污水的脱氮性能[J].环境科学,2018,39(4):1-16.
[15]CHEN G H,LI J,SALMA T,et al.Anaerobic ammonium oxidation(ANAMMOX)sludge immobilized by waterborne polyurethane and its nitrogen removal performance-a lab scale study[J].Rsc Advances,2015,5(32):25372-25381.
[16]NOZHEVNIKOVA A,YU L,NEKRASOVA V K,et al.Anaerobic ammonium oxidation(Anammox)in immobilized activated sludge biofilms during the treatment of weak wastewater[J].Microbiology,2012,81(1):25-34.
[17]高华崇,乔丽丽,尹莉,等.包埋微生物固定化载体的结构性能研究[J].能源环境保护,2017,31(1):29-33.
[18]朱曦,衣萌萌,王淼,等.微生物固定化载体筛选及其水质处理效果研究[J].淡水渔业,2017,47(3):58-65.
[19]ZHU G L,HU Y Y,WANG Q R.Nitrogen removal performance of anaerobic ammonia oxidation co-culture immobilized in different gel carriers[J].Water Science and Technology,2009,59(12):2379-2386.
[20]Han W,Hu Y Y,Li S Y,et al.Effect of organic loading rate on dark fermentative hydrogen production in the continuous stirred tank reactor and continuous mixed immobilized sludge reactor from waste pastry hydrolysate[J].Waste Management,2016,58:335-340.
[21]YANG H,GUAN Q.Study on the diffusion coefficients for ammonia nitrogen and nitrite and nitrate in PVA gels[J].Water Science and Technology,2016,74(8):1773-1779.
[22]蔡昌凤,梁磊.混合固定化硝化菌和好氧反硝化菌处理焦化废水[J].环境工程学报,2009,30(8):1391-1394.
[23]于濛雨,刘毅,田玉斌,等.包埋氨氧化细菌短程硝化的高效稳定运行[J].环境科学,2017,38(7):2925-2930.
[24]李辉军.包埋氨氧化细菌处理合成氨工业废水的研究[D].南京:南京理工大学,2014.
[25]陈光辉,李军,邓海亮,等.厌氧氨氧化污泥包埋固定化及其脱氮效能[J].北京工业大学学报,2015(4):612-620.
[26]边阁,徐笑笑,苗敬芝,等.共固定化亚硝化-反硝化细菌工艺及特性研究[J].湖北农业科学,2016(1):170-175.
[27]MA F,SUN Y,LI A,et al.Activation of accumulated nitrite reduction by immobilized Pseudomonas stutzeri T13 during aerobic denitrification[J].Bioresource Technology,2015,187:30-36.
[28]MAGRI A,VANOTTI M B,SZOEGI A A.Anammox sludge immobilized in polyvinyl alcohol(PVA)cryogel carriers[J].Bioresource Technology,2012,114:231-240.
[29]魣LAMO A C D,PARIENTE M I,VASILIADOU I,et al.Removal of pharmaceutical compounds from urban wastewater by an advanced bio-oxidation process based on fungi Trametes versicolor immobilized in a continuous RBC system[J].Environmental Science and Pollution Research International,2017,10:1-9.
[30]RAMLI N M,VERDEGEM M C J,YUSOFF F M,et al.Removal of ammonium and nitrate in recirculating aquaculture systems by the epiphyte Stigeoclonium nanum immobilized in alginate beads[J].Aquaculture Environment Interactions,2017,9:213-222.
[31]杨宏,胡银龙.硝化细菌的培养及包埋固定化中试[J].环境科学,2018,39(6):1-11.
[32]ZINATIZADEH A A,MOHAMMADI P,MIRGHORAYSHI M,et al.An anaerobic hybrid bioreactor of granular and immobilized biomass for anaerobic digestion(AD)and dark fermentation(DF)of palm oil mill effluent:Mass transfer evaluation in granular sludge and role of internal packing[J].Biomass&Bioenergy,2017,103:1-10.
[33]USHANI U,BANU J R,KAVITHA S,et al.Immobilized and Mg SO4induced cost effective bacterial disintegration of waste activated sludge for effective anaerobic digestion[J].Chemosphere,2017,175:66-75.
[34]许琼琳,陈珍妮.一种固定化微生物处理高氨氮及有机废水系统:CN206538256U[P].2017-10-03.
[35]刘波,盛世雄,杜凌峰,等.一种固定化厌氧氨氧化菌泥的制备方法及其用于污水处理的方法:CN107162187A[P].2017-09-15.
[36]胡安杨,刘勇,何志群.一种聚乙烯醇固定化微生物凝胶处理污水的曝气装置:CN206457309U[P].2017-09-01.
[37]ZENG M,HU J,WANG D H,et al.Improving a compact biofilm reactor to realize efficient nitrogen removal performance:step-feed,intermittent aeration,and immobilization technique[J].Environmental Science and Pollution Research,2018,25(7):6240-6250.
[38]SEIFAN M,SAMANI A K,HEWITT S,et al.The effect of cell immobilization by calcium alginate on bacterially induced calcium carbonate precipitation[J].Fermentation-Basel,2017,3(4):57.
[39]许晓毅,尤晓露,吕晨培,等.包埋固定化活性污泥脱氮特性与微生物群落分析[J].环境科学,2017,38(5):2052-2058.
[40]DONG H H,WANG W,SONG Z Z,et al.A high-efficiency denitrification bioreactor for the treatment of acrylonitrile wastewater using waterborne polyurethane immobilized activated sludge[J].Bioresource Technology,2017,239:472-481.
[41]邵勇,赵茂杰,曾文超,等.包埋固定化硝化菌剂处理合成氨工业废水[J].给水排水,2017(4):68-73.
[42]乔丽丽,李璐,贾琰,等.固定化氨氮降解菌处理氨氮废水的试验研究[J].环境污染与防治,2017,39(7):784-788.
[43]SEKOA P T,AWOSUSI A A,YORO K O,et al.Microbial cell immobilization in biohydrogen production:a short overview[J].Critical Reviews in Biotechnology,2018,38(2):157-171.
[44]WANG H,JIN L,CHENG H,et al.Removal of phenol and chlorophenols in aqueous solutions by immobilizated polyphenol oxidase[J].Environmental Chemistry,2015,34(3):571-577.
[45]JIANG J,LIU Y,LIU Y,et al.Novel Zn ONPs/PVA-functionalized biomaterials for bacterial cells immobilization and its strengthening effects on quinoline biodegradation[J].Current Microbiology,2018,75(3):316-322.
[46]SATAR I.Immobilized mixed-culture reactor(IMc R)for hydrogen and methane production from glucose[J].Energy,2017,139:1188-1196.
[47]ZHANG K,LIU Y,LUO H,et al.Bacterial community dynamics and enhanced degradation of di-n-octyl phthalate(DOP)by corncobsodium alginate immobilized bacteria[J].Geoderma,2017,305:264-274.
[48]SHEN Y,GAO J,LI L.Municipal wastewater treatment via coimmobilized microalgal-bacterial symbiosis:Microorganism growth and nutrients removal[J].Bioresource Technology,2017,243:905-913.
[49]JIANG Y,YANG K,DENG T,et al.Immobilization of halophilic yeast for effective removal of phenol in hypersaline conditions[J].Water Science and Technology,2018,77(3):706-713.
[50]MUJTABA G,LEE K.Treatment of real wastewater using co-culture of immobilized Chlorella vulgaris and suspended activated sludge[J].Water Research,2017,120:174-184.
[51]KUMAR A,SKS P,MARDAN B,et al.Immobilization of xylanase using a protein-inorganic hybrid system[J].Journal of Microbiology and Biotechnology,2018,28(4):638-644.
[52]TRELLES J A,LAPPONIAB M J.Immobilization techniques applied to the development of biocatalysts for the synthesis of nucleoside analogue derivatives[J].Current Pharmaceutical Design,2017,24(45):6879-6897.
[53]BELGRANO F,DIEGEL O,PEREIRA J N,et al.Cell immobilization on 3D-printed matrices:A model study on propionic acid fermentation[J].Bioresource Technology,2018,249:777-782.
[54]CHAO C,GUAN H,ZHANG J,et al.Immobilization of laccase onto porous polyvinyl alcohol/halloysite hybrid beads for dye removal[J].Water Science and Technology,2018,77(3):809-818.
[2]NAKANO K,IWASAWA H,ITO O,et al.Improved simultaneous nitrification and denitrification in a single reactor by using two different immobilization carriers with specific oxygen transfer characteristics[J].Bioprocess and Biosystems Engineering,2004,26(3):141-145.
[3]SUN S Y,FAN S L,SHEN K X,et al.Laboratory assessment of bioleaching of shallow eutrophic sediment by immobilized photosynthetic bacteria[J].Environmental Science and Pollution Research2017,24(28):22143-22151.
[4]SUN S Y,FAN S L,SHEN K X,et al.Tannin-immobilized cellulose microspheres as effective adsorbents for removing cationic dye(Methylene Blue)from aqueous solution[J].Journal of Chemical Technology and Biotechnology,2017,92(6):1276-1284.
[5]陈珍妮,唐明阳.一种固定化微生物处理高氨氮及有机废水系统CN106587353A[P].2017-04-26.
[6]HERKOMMEROV魣K,ZEMAN魱KOV魣J,SYCHROV魣H,et al Immobilization in polyvinyl alcohol hydrogel enhances yeast storage stability and reusability of recombinant laccase-producing S-cerevisiae[J].Biotechnology Letters,2018,40(2):405-411.
[7]ZUKI S N S M,Tan L L,AZMI N S,et al.A whole cell bio-optode based on immobilized nitrite-degrading microorganism on the acrylic microspheres for visual quantitation of nitrite ion[J].Sensors and Actuators B-Chemical,2018,255:2844-2852.
[8]RAJESH BANU J,USHANIA U,RAJKUMARB M,et al.Impac of mild alkali dosage on immobilized Exiguobacterium spp.mediated cost and energy efficient sludge disi ntegration[J].Bioresource Technology,2017,245:434-441.
[9]DONG W J,LU G,YAN L,et al.Characteristics of pellets with immobilized activated sludge and its performance in increasing nitrification in sequencing batch reactors at low temperatures[J].Journal of Environmental Sciences,2016,42:202-209.
[10]NISHIDA V S,OLIVEIRA DE R F,BRUGNARI T,et al.Immobilization of Aspergillus awamori beta-glucosidase on commercial gelatin:An inexpensive and efficient process[J].International Journal of Biological Macromolecules,2018,111:1206-1213.
[11]AKPOR O B.Effect of nitrogen source concentration on decolouration rates of laboratory dyes by immobilized cells of two bacterial species[J].World Journal of Microbiology&Biotechnology,2018,34(1):18
[12]LIU Y K,MAO Y,WANG J.Methods and application of immobilized microorganism in PVA[J].Science&Technology Review,2016,34(22):56-61.
[13]ZDARTA J,EDRZAK A J,KLAPISZEWSKI覵,et al.Immobilization of Cellulase on a functional inorganic-organic hybrid support:stability and kinetic study[J].Catalysts,2017,7(12)374.
[14]单晓静,于德爽,李津,等.海水优化ANAMMOX包埋固定化及其处理含海水污水的脱氮性能[J].环境科学,2018,39(4):1-16.
[15]CHEN G H,LI J,SALMA T,et al.Anaerobic ammonium oxidation(ANAMMOX)sludge immobilized by waterborne polyurethane and its nitrogen removal performance-a lab scale study[J].Rsc Advances,2015,5(32):25372-25381.
[16]NOZHEVNIKOVA A,YU L,NEKRASOVA V K,et al.Anaerobic ammonium oxidation(Anammox)in immobilized activated sludge biofilms during the treatment of weak wastewater[J].Microbiology,2012,81(1):25-34.
[17]高华崇,乔丽丽,尹莉,等.包埋微生物固定化载体的结构性能研究[J].能源环境保护,2017,31(1):29-33.
[18]朱曦,衣萌萌,王淼,等.微生物固定化载体筛选及其水质处理效果研究[J].淡水渔业,2017,47(3):58-65.
[19]ZHU G L,HU Y Y,WANG Q R.Nitrogen removal performance of anaerobic ammonia oxidation co-culture immobilized in different gel carriers[J].Water Science and Technology,2009,59(12):2379-2386.
[20]Han W,Hu Y Y,Li S Y,et al.Effect of organic loading rate on dark fermentative hydrogen production in the continuous stirred tank reactor and continuous mixed immobilized sludge reactor from waste pastry hydrolysate[J].Waste Management,2016,58:335-340.
[21]YANG H,GUAN Q.Study on the diffusion coefficients for ammonia nitrogen and nitrite and nitrate in PVA gels[J].Water Science and Technology,2016,74(8):1773-1779.
[22]蔡昌凤,梁磊.混合固定化硝化菌和好氧反硝化菌处理焦化废水[J].环境工程学报,2009,30(8):1391-1394.
[23]于濛雨,刘毅,田玉斌,等.包埋氨氧化细菌短程硝化的高效稳定运行[J].环境科学,2017,38(7):2925-2930.
[24]李辉军.包埋氨氧化细菌处理合成氨工业废水的研究[D].南京:南京理工大学,2014.
[25]陈光辉,李军,邓海亮,等.厌氧氨氧化污泥包埋固定化及其脱氮效能[J].北京工业大学学报,2015(4):612-620.
[26]边阁,徐笑笑,苗敬芝,等.共固定化亚硝化-反硝化细菌工艺及特性研究[J].湖北农业科学,2016(1):170-175.
[27]MA F,SUN Y,LI A,et al.Activation of accumulated nitrite reduction by immobilized Pseudomonas stutzeri T13 during aerobic denitrification[J].Bioresource Technology,2015,187:30-36.
[28]MAGRI A,VANOTTI M B,SZOEGI A A.Anammox sludge immobilized in polyvinyl alcohol(PVA)cryogel carriers[J].Bioresource Technology,2012,114:231-240.
[29]魣LAMO A C D,PARIENTE M I,VASILIADOU I,et al.Removal of pharmaceutical compounds from urban wastewater by an advanced bio-oxidation process based on fungi Trametes versicolor immobilized in a continuous RBC system[J].Environmental Science and Pollution Research International,2017,10:1-9.
[30]RAMLI N M,VERDEGEM M C J,YUSOFF F M,et al.Removal of ammonium and nitrate in recirculating aquaculture systems by the epiphyte Stigeoclonium nanum immobilized in alginate beads[J].Aquaculture Environment Interactions,2017,9:213-222.
[31]杨宏,胡银龙.硝化细菌的培养及包埋固定化中试[J].环境科学,2018,39(6):1-11.
[32]ZINATIZADEH A A,MOHAMMADI P,MIRGHORAYSHI M,et al.An anaerobic hybrid bioreactor of granular and immobilized biomass for anaerobic digestion(AD)and dark fermentation(DF)of palm oil mill effluent:Mass transfer evaluation in granular sludge and role of internal packing[J].Biomass&Bioenergy,2017,103:1-10.
[33]USHANI U,BANU J R,KAVITHA S,et al.Immobilized and Mg SO4induced cost effective bacterial disintegration of waste activated sludge for effective anaerobic digestion[J].Chemosphere,2017,175:66-75.
[34]许琼琳,陈珍妮.一种固定化微生物处理高氨氮及有机废水系统:CN206538256U[P].2017-10-03.
[35]刘波,盛世雄,杜凌峰,等.一种固定化厌氧氨氧化菌泥的制备方法及其用于污水处理的方法:CN107162187A[P].2017-09-15.
[36]胡安杨,刘勇,何志群.一种聚乙烯醇固定化微生物凝胶处理污水的曝气装置:CN206457309U[P].2017-09-01.
[37]ZENG M,HU J,WANG D H,et al.Improving a compact biofilm reactor to realize efficient nitrogen removal performance:step-feed,intermittent aeration,and immobilization technique[J].Environmental Science and Pollution Research,2018,25(7):6240-6250.
[38]SEIFAN M,SAMANI A K,HEWITT S,et al.The effect of cell immobilization by calcium alginate on bacterially induced calcium carbonate precipitation[J].Fermentation-Basel,2017,3(4):57.
[39]许晓毅,尤晓露,吕晨培,等.包埋固定化活性污泥脱氮特性与微生物群落分析[J].环境科学,2017,38(5):2052-2058.
[40]DONG H H,WANG W,SONG Z Z,et al.A high-efficiency denitrification bioreactor for the treatment of acrylonitrile wastewater using waterborne polyurethane immobilized activated sludge[J].Bioresource Technology,2017,239:472-481.
[41]邵勇,赵茂杰,曾文超,等.包埋固定化硝化菌剂处理合成氨工业废水[J].给水排水,2017(4):68-73.
[42]乔丽丽,李璐,贾琰,等.固定化氨氮降解菌处理氨氮废水的试验研究[J].环境污染与防治,2017,39(7):784-788.
[43]SEKOA P T,AWOSUSI A A,YORO K O,et al.Microbial cell immobilization in biohydrogen production:a short overview[J].Critical Reviews in Biotechnology,2018,38(2):157-171.
[44]WANG H,JIN L,CHENG H,et al.Removal of phenol and chlorophenols in aqueous solutions by immobilizated polyphenol oxidase[J].Environmental Chemistry,2015,34(3):571-577.
[45]JIANG J,LIU Y,LIU Y,et al.Novel Zn ONPs/PVA-functionalized biomaterials for bacterial cells immobilization and its strengthening effects on quinoline biodegradation[J].Current Microbiology,2018,75(3):316-322.
[46]SATAR I.Immobilized mixed-culture reactor(IMc R)for hydrogen and methane production from glucose[J].Energy,2017,139:1188-1196.
[47]ZHANG K,LIU Y,LUO H,et al.Bacterial community dynamics and enhanced degradation of di-n-octyl phthalate(DOP)by corncobsodium alginate immobilized bacteria[J].Geoderma,2017,305:264-274.
[48]SHEN Y,GAO J,LI L.Municipal wastewater treatment via coimmobilized microalgal-bacterial symbiosis:Microorganism growth and nutrients removal[J].Bioresource Technology,2017,243:905-913.
[49]JIANG Y,YANG K,DENG T,et al.Immobilization of halophilic yeast for effective removal of phenol in hypersaline conditions[J].Water Science and Technology,2018,77(3):706-713.
[50]MUJTABA G,LEE K.Treatment of real wastewater using co-culture of immobilized Chlorella vulgaris and suspended activated sludge[J].Water Research,2017,120:174-184.
[51]KUMAR A,SKS P,MARDAN B,et al.Immobilization of xylanase using a protein-inorganic hybrid system[J].Journal of Microbiology and Biotechnology,2018,28(4):638-644.
[52]TRELLES J A,LAPPONIAB M J.Immobilization techniques applied to the development of biocatalysts for the synthesis of nucleoside analogue derivatives[J].Current Pharmaceutical Design,2017,24(45):6879-6897.
[53]BELGRANO F,DIEGEL O,PEREIRA J N,et al.Cell immobilization on 3D-printed matrices:A model study on propionic acid fermentation[J].Bioresource Technology,2018,249:777-782.
[54]CHAO C,GUAN H,ZHANG J,et al.Immobilization of laccase onto porous polyvinyl alcohol/halloysite hybrid beads for dye removal[J].Water Science and Technology,2018,77(3):809-818.
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