硅质钝化材料与促生菌剂组合施用对水稻累积镉效应研究
|
摘要
在镉污染土壤的钝化修复应用中,由于钝化材料一般不具有特异选择性,在钝化重金属的同时往往会同步钝化土壤中的养分,从而导致作物的生长受到抑制。基于课题组前期研发的对Cd具有强吸附能力的改性钼矿和改性泥岩等两种硅质材料和一种兼具解磷、促生长作用的促生芽孢杆菌菌剂在田间试验条件下验证其降低水稻累积Cd效应的影响,并对钝化材料和功能菌剂的组合应用效果进行评价。结果表明,未添加促生菌剂的处理下土壤有效磷含量与对照组之间无显著差异,而单施促生菌剂及其与改性泥岩组合处理均显著增加了土壤的有效磷含量,增幅分别为21.6%和37.8%。各修复处理对稻米产量的增产效果排序为:改性泥岩+促生菌剂>促生菌剂>改性泥岩>改性钼矿,增产幅度为0.5%-8.8%,其中改性泥岩+促生菌剂的增产效果达到显著水平,而改性钼矿+促生菌剂未表现出增产效应。各修复处理均显著地降低了稻米中Cd含量,降低效果排序为:改性钼矿>改性钼矿+促生菌剂>改性泥岩>改性泥岩+促生菌剂>促生菌剂,降低幅度为35.3%-65.0%。除单独施用促生菌剂处理外,其他处理实施后水稻籽粒Cd含量均低于国家食品安全标准限量值(0.2 mg·kg~(-1))。采用的改性钼矿的作用机理主要是通过降低Cd从土壤向根系的迁移,而改性泥岩的作用机理处理降低Cd从土壤向根系的迁移外,还会通过增加Cd在茎叶中的滞留效应达到降低籽粒Cd累积的效果。综合组合处理对土壤养分的活化和对水稻的促生效果,在该研究试验用地上,具有弱碱性的改性泥岩与促生菌剂的技术集成是一种兼具促生和降低Cd在水稻中累积效应的Cd污染稻田安全利用技术模式。
In most applications of passivation remediation of cadmium-contaminated soils, soil nutrients would be simultaneously immobilized since generally-used passivants don't have specific selectivity on heavy metals, which may have a negative effect on plant growth. Two siliceous materials, modified molybdenum ore and modified mudstone, which were previous developed by our group, were employed in this study, as well as a growth-promoting Bacillus inoculum. Field studies were conducted to investigate the effect of single/combined remediation measures on Cd accumulation in rice grain. The results indicated that there was no significant difference in soil available phosphorus content(Olsen-P) between the control group and the treatments without Bacillus inoculum.Treatments ‘Bacillus inoculum' and ‘Bacillus inoculum+Modified mudstone' significantly increased soil Olsen-P by 21.6% and37.8%, respectively. The order of the effect of different remediation treatments on rice yield was: Modified mudstone+Bacillus inoculum>Bacillus inoculum>Modified mudstone>Modified molybdenum ore. The yield-increasing range was 0.5%-8.8%. Among them, the yield-increasing effect of ‘modified mudstone+Bacillus inoculum' reached a significant level, while no significant effect was found under the treatment of modified molybdenum ore+Bacillus inoculum. Cd accumulation in rice grain were significantly reduced under all treatments. The order of reduction effect was: modified molybdenum ore>modified molybdenum ore+Bacillus inoculum>modified mudstone>modified mudstone+Bacillus inoculum>Bacillus inoculum. The decrease range were 35.3%-65.0%.The Cd content in rice grains was lower than the national food safety standard limit(0.2 mg·kg~(-1)) under all treatments except for‘Bacillus inoculum'. In this study, modified molybdenum ore may reduce Cd in rice grain through inhibiting effect of Cd migrate from soil to rice root, while modified mudstone may reduce the accumulation of Cd by increasing the retention effect of Cd in rice shoot(stems and leaves). By comprehensive consideration of remediation and growth promoting effects, an integrative technique of alkalescent modified mudstone and Bacillus inoculum is an effective passivation method and suitable for extensive application in Pearl River Delta.
In most applications of passivation remediation of cadmium-contaminated soils, soil nutrients would be simultaneously immobilized since generally-used passivants don't have specific selectivity on heavy metals, which may have a negative effect on plant growth. Two siliceous materials, modified molybdenum ore and modified mudstone, which were previous developed by our group, were employed in this study, as well as a growth-promoting Bacillus inoculum. Field studies were conducted to investigate the effect of single/combined remediation measures on Cd accumulation in rice grain. The results indicated that there was no significant difference in soil available phosphorus content(Olsen-P) between the control group and the treatments without Bacillus inoculum.Treatments ‘Bacillus inoculum' and ‘Bacillus inoculum+Modified mudstone' significantly increased soil Olsen-P by 21.6% and37.8%, respectively. The order of the effect of different remediation treatments on rice yield was: Modified mudstone+Bacillus inoculum>Bacillus inoculum>Modified mudstone>Modified molybdenum ore. The yield-increasing range was 0.5%-8.8%. Among them, the yield-increasing effect of ‘modified mudstone+Bacillus inoculum' reached a significant level, while no significant effect was found under the treatment of modified molybdenum ore+Bacillus inoculum. Cd accumulation in rice grain were significantly reduced under all treatments. The order of reduction effect was: modified molybdenum ore>modified molybdenum ore+Bacillus inoculum>modified mudstone>modified mudstone+Bacillus inoculum>Bacillus inoculum. The decrease range were 35.3%-65.0%.The Cd content in rice grains was lower than the national food safety standard limit(0.2 mg·kg~(-1)) under all treatments except for‘Bacillus inoculum'. In this study, modified molybdenum ore may reduce Cd in rice grain through inhibiting effect of Cd migrate from soil to rice root, while modified mudstone may reduce the accumulation of Cd by increasing the retention effect of Cd in rice shoot(stems and leaves). By comprehensive consideration of remediation and growth promoting effects, an integrative technique of alkalescent modified mudstone and Bacillus inoculum is an effective passivation method and suitable for extensive application in Pearl River Delta.
引文
ATAR N,OLGUN A,WANG S B,2012.Adsorption of cadmium(II)and zinc(II)on boron enrichment process waste in aqueous solutions:Batch and fixed-bed system studies[J].Chemical Engineering Journal,192:1-7.
BOLAN N,KUNHIKRISHNAN A,THANGARAJAN R,et al.,2014.Remediation of heavy metal(loid)s contaminated soils-To mobilize or to immobilize?[J].Journal of Hazardous Materials,266:141-166.
BRADL H B,2004.Adsorption of heavy metal ions on soils and soils constituents[J].Journal of Colloid and Interface Science,277(1):1-18.
HE S R,LI Y T,WENG L P,et al.,2018.Competitive adsorption of Cd2+,Pb2+and Ni2+onto Fe3+-modified argillaceous limestone:Influence of pH,ionic strength and natural organic matters[J].Science of The Total Environment,637-638:69-78.
HOUBA V J G,TEMMINGHOFF E J M,GAIKHORST G A,et al.,2000.Soil analysis procedures using 0.01 M calcium chloride as extraction reagent[J].Communications in Soil Science and Plant Analysis,31(9-10):1299-1396.
HUANG G Y,SU X J,RIZWAN M S,et al.,2016.Chemical immobilization of Pb,Cu,and Cd by phosphate materials and calcium carbonate in contaminated soils[J].Environmental Science and Pollution Research,23(16):16845-16856.
KHAN M S,ZAIDI A,WANI P A,2007.Role of phosphate-solubilizing microorganisms in sustainable agriculture-A review[J].Agronomy for Sustainable Development,27(1):29-43.
KUMPIENE J,LAGERKVIST A,MAURICE C,2008.Stabilization of As,Cr,Cu,Pb and Zn in soil using amendments:A review[J].Waste Management,28(1):215-225.
LI D Q,LI W Y,LU Q,et al.,2018.Cadmium bioavailability well assessed by DGT and factors influencing cadmium accumulation in rice grains from paddy soils of three parent materials[J].Journal of Soils and Sediments,18(7):2552-2561.
LIANG Y C,SUN W C,ZHU Y G,et al.,2007.Mechanisms of silicon-mediated alleviation of abiotic stresses in higher plants:Areview[J].Environmental Pollution,147(2):422-428.
LIU J G,ZHANG H M,ZHANG Y X,et al.,2013.Silicon attenuates cadmium toxicity in Solanum nigrum L.by reducing cadmium uptake and oxidative stress[J].Plant Physiology and Biochemistry,68:1-7.
MADEJON E,MADEJON P,BURGOS P,et al.,2009.Trace elements,pHand organic matter evolution in contaminated soils under assisted natural remediation:A 4-year field study[J].Journal of Hazardous Materials,162(2-3):931-938.
MAO X Y,WANG L,GU S Q,et al.,2018.Synthesis of a three-dimensional network sodium alginate-poly(acrylic acid)/attapulgite hydrogel with good mechanic property and reusability for efficient adsorption of Cu2+and Pb2+[J].Environmental Chemistry Letters,16(2):653-658.
MAZóN-MONTIJO D A,SOTELO-LERMA M,RODRíGUEZ-FERNáNDEZ L,et al.,2010.AFM,XPS and RBS studies of the growth process of CdS thin films on ITO/glass substrates deposited using an ammonia-free chemical process[J].Applied Surface Science,256(13):4280-4287.
OLIVEIRA C A,ALVES V M C,MARRIEL I E,et al.,2009.Phosphate solubilizing microorganisms isolated from rhizosphere of maize cultivated in an oxisol of the Brazilian Cerrado Biome[J].Soil Biology and Biochemistry,41(9):1782-1787.
RICHARDSON A E,2001.Prospects for using soil microorganisms to improve the acquisition of phosphorus by plants[J].Functional Plant Biology,28(9):897-906.
RODRíGUEZ H,FRAGA R,1999.Phosphate solubilizing bacteria and their role in plant growth promotion[J].Biotechnology Advances,17(4):319-339.
SATARUG S,BAKER J R,URBENJAPOL S,et al.,2003.A global perspective on cadmium pollution and toxicity in non-occupationally exposed population[J].Toxicology Letters,137(1):65-83.
SONG L M,ZHANG S J,2009.Formation ofα-Fe2O3/FeOOHnanostructures with various morphologies by a hydrothermal route and their photocatalytic properties[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects,348(1-3):217-220.
WANG J J,ZENG X B,ZHANG H,et al.,2018.Effect of exogenous phosphate on the lability and phytoavailability of arsenic in soils[J].Chemosphere,196:540-547.
WANG J,LIN X Y,LUO X G,et al.,2014.A sorbent of carboxymethyl cellulose loaded with zirconium for the removal of fluoride from aqueous solution[J].Chemical Engineering Journal,252:415-422.
WU Y J,ZHOU H,ZOU Z J,et al.,2016.A three-year in-situ study on the persistence of a combined amendment(limestone+sepiolite)for remedying paddy soil polluted with heavy metals[J].Ecotoxicology and Environmental Safety,130:163-170.
XU S X,ZHAO Y C,WANG M Y,et al.,2018.Comparison of multivariate methods for estimating selected soil properties from intact soil cores of paddy fields by Vis-NIR spectroscopy[J].Geoderma,310:29-43.
XU Y,LIANG X F,XU Y M,et al.,2017.Remediation of heavy metal-polluted agricultural soils using clay minerals:A review[J].Pedosphere,27(2):193-204.
YAO A J,WANG Y,LING X D,et al.,2017.Effects of an iron-silicon material,a synthetic zeolite and an alkaline clay on vegetable uptake of As and Cd from a polluted agricultural soil and proposed remediation mechanisms[J].Environmental Geochemistry and Health,39(2):353-367.
YU X,LIU X,ZHU T H,et al.,2011.Isolation and characterization of phosphate-solubilizing bacteria from walnut and their effect on growth and phosphorus mobilization[J].Biology and Fertility of Soils,47(4):437-446.
陈展祥,陈传胜,陈卫平,等,2018.凹凸棒石及其改性材料对土壤镉生物有效性的影响与机制[J].环境科学,39(10):4744-4751.CHEN Z X,CHEN C S,CHEN W P,et al.,2018.Effect and mechanism of attapulgite and its modified materials on bioavailability of cadmium in soil[J].Environmental Science,39(10):4744-4751.
窦磊,杜海燕,游远航,等,2014.珠江三角洲经济区生态地球化学评价[J].现代地质,28(5):915-927.DOU L,DU H Y,YOU Y H,et al.,2014.Eco-geochemical survey and assessment in Pearl River Delta economic zone,Guangdong Province,China[J].Geoscience,28(5):915-927.
胡峥,2017.钼尾砂改性及其对酸性土壤镉的钝化机制研究[D].广州:华南农业大学:12-25.HU Z,2017.Modification of molybdenum tailing and its immobilizing mechanism on cadmium in acid soil[D].Guangzhou:South China Agricultural University:12-25.
李冬琴,2018.水分调控下Fe和DOM对重金属高背景土壤Cd活性形态的影响机制[D].广州:华南农业大学:18-20.LI D Q,2018.Effect mechanisms of Fe and DOM on Cd lability in high heavy metal background soil under water regulations[D].Guangzhou:South China Agricultural University:18-20.
林钰栅,范缙,蔡邦平,等,2016.解磷微生物在重金属污染原位修复中的作用及其机理研究进展[J].厦门大学学报(自然科学版),55(5):697-706.LIN Y S,FAN J,CAI B P,et al.,2016.Progress on roles and mechanisms of phosphate-solubilizing microorganisms in remediation of heavy metal contaminated soils[J].Journal of Xiamen University(Natural Science),55(5):697-706.
蒲强,2017.多功能芽孢杆菌筛选及其促生长和吸附镉效果与机理研究[D].广州:华南农业大学:9-31.PU Q,2017.Study on screening of multifunctional bacillus and its effect and mechanism on plant growth and biosorption of cadmium[D].Guangzhou:South China Agricultural University:9-31.
王进进,杨行健,胡峥,等,2019.基于风险等级的重金属污染耕地土壤修复技术集成体系研究[J].农业环境科学学报,38(2):249-256.WANG J J,YANG X J,HU Z,et al.,2019.Research on the risk level-based technology integration for the remediation of heavy metals polluted farmland[J].Journal of Agro-Environment Science,38(2):249-256.
BOLAN N,KUNHIKRISHNAN A,THANGARAJAN R,et al.,2014.Remediation of heavy metal(loid)s contaminated soils-To mobilize or to immobilize?[J].Journal of Hazardous Materials,266:141-166.
BRADL H B,2004.Adsorption of heavy metal ions on soils and soils constituents[J].Journal of Colloid and Interface Science,277(1):1-18.
HE S R,LI Y T,WENG L P,et al.,2018.Competitive adsorption of Cd2+,Pb2+and Ni2+onto Fe3+-modified argillaceous limestone:Influence of pH,ionic strength and natural organic matters[J].Science of The Total Environment,637-638:69-78.
HOUBA V J G,TEMMINGHOFF E J M,GAIKHORST G A,et al.,2000.Soil analysis procedures using 0.01 M calcium chloride as extraction reagent[J].Communications in Soil Science and Plant Analysis,31(9-10):1299-1396.
HUANG G Y,SU X J,RIZWAN M S,et al.,2016.Chemical immobilization of Pb,Cu,and Cd by phosphate materials and calcium carbonate in contaminated soils[J].Environmental Science and Pollution Research,23(16):16845-16856.
KHAN M S,ZAIDI A,WANI P A,2007.Role of phosphate-solubilizing microorganisms in sustainable agriculture-A review[J].Agronomy for Sustainable Development,27(1):29-43.
KUMPIENE J,LAGERKVIST A,MAURICE C,2008.Stabilization of As,Cr,Cu,Pb and Zn in soil using amendments:A review[J].Waste Management,28(1):215-225.
LI D Q,LI W Y,LU Q,et al.,2018.Cadmium bioavailability well assessed by DGT and factors influencing cadmium accumulation in rice grains from paddy soils of three parent materials[J].Journal of Soils and Sediments,18(7):2552-2561.
LIANG Y C,SUN W C,ZHU Y G,et al.,2007.Mechanisms of silicon-mediated alleviation of abiotic stresses in higher plants:Areview[J].Environmental Pollution,147(2):422-428.
LIU J G,ZHANG H M,ZHANG Y X,et al.,2013.Silicon attenuates cadmium toxicity in Solanum nigrum L.by reducing cadmium uptake and oxidative stress[J].Plant Physiology and Biochemistry,68:1-7.
MADEJON E,MADEJON P,BURGOS P,et al.,2009.Trace elements,pHand organic matter evolution in contaminated soils under assisted natural remediation:A 4-year field study[J].Journal of Hazardous Materials,162(2-3):931-938.
MAO X Y,WANG L,GU S Q,et al.,2018.Synthesis of a three-dimensional network sodium alginate-poly(acrylic acid)/attapulgite hydrogel with good mechanic property and reusability for efficient adsorption of Cu2+and Pb2+[J].Environmental Chemistry Letters,16(2):653-658.
MAZóN-MONTIJO D A,SOTELO-LERMA M,RODRíGUEZ-FERNáNDEZ L,et al.,2010.AFM,XPS and RBS studies of the growth process of CdS thin films on ITO/glass substrates deposited using an ammonia-free chemical process[J].Applied Surface Science,256(13):4280-4287.
OLIVEIRA C A,ALVES V M C,MARRIEL I E,et al.,2009.Phosphate solubilizing microorganisms isolated from rhizosphere of maize cultivated in an oxisol of the Brazilian Cerrado Biome[J].Soil Biology and Biochemistry,41(9):1782-1787.
RICHARDSON A E,2001.Prospects for using soil microorganisms to improve the acquisition of phosphorus by plants[J].Functional Plant Biology,28(9):897-906.
RODRíGUEZ H,FRAGA R,1999.Phosphate solubilizing bacteria and their role in plant growth promotion[J].Biotechnology Advances,17(4):319-339.
SATARUG S,BAKER J R,URBENJAPOL S,et al.,2003.A global perspective on cadmium pollution and toxicity in non-occupationally exposed population[J].Toxicology Letters,137(1):65-83.
SONG L M,ZHANG S J,2009.Formation ofα-Fe2O3/FeOOHnanostructures with various morphologies by a hydrothermal route and their photocatalytic properties[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects,348(1-3):217-220.
WANG J J,ZENG X B,ZHANG H,et al.,2018.Effect of exogenous phosphate on the lability and phytoavailability of arsenic in soils[J].Chemosphere,196:540-547.
WANG J,LIN X Y,LUO X G,et al.,2014.A sorbent of carboxymethyl cellulose loaded with zirconium for the removal of fluoride from aqueous solution[J].Chemical Engineering Journal,252:415-422.
WU Y J,ZHOU H,ZOU Z J,et al.,2016.A three-year in-situ study on the persistence of a combined amendment(limestone+sepiolite)for remedying paddy soil polluted with heavy metals[J].Ecotoxicology and Environmental Safety,130:163-170.
XU S X,ZHAO Y C,WANG M Y,et al.,2018.Comparison of multivariate methods for estimating selected soil properties from intact soil cores of paddy fields by Vis-NIR spectroscopy[J].Geoderma,310:29-43.
XU Y,LIANG X F,XU Y M,et al.,2017.Remediation of heavy metal-polluted agricultural soils using clay minerals:A review[J].Pedosphere,27(2):193-204.
YAO A J,WANG Y,LING X D,et al.,2017.Effects of an iron-silicon material,a synthetic zeolite and an alkaline clay on vegetable uptake of As and Cd from a polluted agricultural soil and proposed remediation mechanisms[J].Environmental Geochemistry and Health,39(2):353-367.
YU X,LIU X,ZHU T H,et al.,2011.Isolation and characterization of phosphate-solubilizing bacteria from walnut and their effect on growth and phosphorus mobilization[J].Biology and Fertility of Soils,47(4):437-446.
陈展祥,陈传胜,陈卫平,等,2018.凹凸棒石及其改性材料对土壤镉生物有效性的影响与机制[J].环境科学,39(10):4744-4751.CHEN Z X,CHEN C S,CHEN W P,et al.,2018.Effect and mechanism of attapulgite and its modified materials on bioavailability of cadmium in soil[J].Environmental Science,39(10):4744-4751.
窦磊,杜海燕,游远航,等,2014.珠江三角洲经济区生态地球化学评价[J].现代地质,28(5):915-927.DOU L,DU H Y,YOU Y H,et al.,2014.Eco-geochemical survey and assessment in Pearl River Delta economic zone,Guangdong Province,China[J].Geoscience,28(5):915-927.
胡峥,2017.钼尾砂改性及其对酸性土壤镉的钝化机制研究[D].广州:华南农业大学:12-25.HU Z,2017.Modification of molybdenum tailing and its immobilizing mechanism on cadmium in acid soil[D].Guangzhou:South China Agricultural University:12-25.
李冬琴,2018.水分调控下Fe和DOM对重金属高背景土壤Cd活性形态的影响机制[D].广州:华南农业大学:18-20.LI D Q,2018.Effect mechanisms of Fe and DOM on Cd lability in high heavy metal background soil under water regulations[D].Guangzhou:South China Agricultural University:18-20.
林钰栅,范缙,蔡邦平,等,2016.解磷微生物在重金属污染原位修复中的作用及其机理研究进展[J].厦门大学学报(自然科学版),55(5):697-706.LIN Y S,FAN J,CAI B P,et al.,2016.Progress on roles and mechanisms of phosphate-solubilizing microorganisms in remediation of heavy metal contaminated soils[J].Journal of Xiamen University(Natural Science),55(5):697-706.
蒲强,2017.多功能芽孢杆菌筛选及其促生长和吸附镉效果与机理研究[D].广州:华南农业大学:9-31.PU Q,2017.Study on screening of multifunctional bacillus and its effect and mechanism on plant growth and biosorption of cadmium[D].Guangzhou:South China Agricultural University:9-31.
王进进,杨行健,胡峥,等,2019.基于风险等级的重金属污染耕地土壤修复技术集成体系研究[J].农业环境科学学报,38(2):249-256.WANG J J,YANG X J,HU Z,et al.,2019.Research on the risk level-based technology integration for the remediation of heavy metals polluted farmland[J].Journal of Agro-Environment Science,38(2):249-256.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |