鸡枞菌多糖对鲫的生长性能、抗氧化及免疫功能的影响
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摘要
为了探讨鸡枞菌多糖(TAP)对鲫(Carassius auratus)的生物学活性,以饲料里添加1%的黄芪多糖(APS)为对照,连续14 d在鲫饲料里分别添加0.1%、0.5%和1%的TAP,观察其在第7和14天对鲫肥满度、肝体比、头肾体比、肝胰脏中MDA、CAT、SOD以及LZM的影响。结果显示:实验第7天,TAP高剂量组的肥满度极显著高于其余各组,其余各组在实验期间的肥满度与肝体比均无显著差异。与空白组比,实验第7天,APS组与TAP中剂量组的肝胰脏MDA含量分别极显著和显著降低;在给药期间两种多糖均使CAT和SOD水平有不同程度的升高。与空白组比,给药期间,TAP和APS均能不同程度提高鲫头肾体比,且二者差异不显著;TAP高剂量组的LZM水平显著提高,且显著高于APS组。结果表明,TAP对鲫连续给药14 d的促生长作用不显著,但可通过上调CAT、SOD来降低MDA,且对非特异性免疫功能有显著提高。
The biologic activity of Termitornyces albuminosus polysaccharide(TAP)in Carassius auratus was investigated in this research.The C.auratus in APS group were fed diet with 1% APS,and in three ATP groups were fed diet with 0.1%、0.5% and 1% ATP,respectively.The fish fullness,hepatopancreas index,head kidney body index,MDA,CAT,SOD and LZM in hepatopancreas of C.auratus were detected in the seventh and fourteenth day after administration.The results showed that the fullness of TAP high dose group was significantly higher than that of other groups at the seventh day compared with the blank group,.There was no significant difference in fullness and hepatopancreas index between the other groups during administration.MDA in hepatopancreas of APS group and TAP medium dose group were significantly decreased compared with blank group.Two kinds of polysaccharides increased the levels of CAT and SOD to varying degrees during the administration.Both TAP and APS could improve the head kidney body index of Carassius auratus in different degrees during administration compared with blank group.The difference between the two groups was not significant.The consequences showed that TAP had no effect on growth promotion during 14 days,but that could decrease MDA by increased CAT and SOD of hepatopancreas and raise the nonspecific immunity function of Carassius auratus.
The biologic activity of Termitornyces albuminosus polysaccharide(TAP)in Carassius auratus was investigated in this research.The C.auratus in APS group were fed diet with 1% APS,and in three ATP groups were fed diet with 0.1%、0.5% and 1% ATP,respectively.The fish fullness,hepatopancreas index,head kidney body index,MDA,CAT,SOD and LZM in hepatopancreas of C.auratus were detected in the seventh and fourteenth day after administration.The results showed that the fullness of TAP high dose group was significantly higher than that of other groups at the seventh day compared with the blank group,.There was no significant difference in fullness and hepatopancreas index between the other groups during administration.MDA in hepatopancreas of APS group and TAP medium dose group were significantly decreased compared with blank group.Two kinds of polysaccharides increased the levels of CAT and SOD to varying degrees during the administration.Both TAP and APS could improve the head kidney body index of Carassius auratus in different degrees during administration compared with blank group.The difference between the two groups was not significant.The consequences showed that TAP had no effect on growth promotion during 14 days,but that could decrease MDA by increased CAT and SOD of hepatopancreas and raise the nonspecific immunity function of Carassius auratus.
引文
[1]陈昌福,姚娟,吴凡华,等.免疫多糖(酵母细胞壁)对受免异育银鲫免疫应答的调节作用[J].淡水渔业,2004,34(4):55-57.
[2]黄玉章.黄芪多糖对奥尼罗非鱼生长性能和免疫功能的影响[D].福州:福建农林大学,2009.
[3]王思芦,汪开毓,耿毅,等.鸡枞菌多糖对小鼠T细胞免疫功能的影响[J].中国兽医科学,2013,43(1):77-83.
[4]王思芦,汪开毓,赵玲,等.鸡枞菌多糖对免疫抑制小鼠免疫功能的影响[J].中国药理学通报,2013,29(1):59-63.
[5]卢彩会,郭晓萌,牟德华.黑皮鸡枞菌水提物和醇提物的主要成分及抗氧化性研究[J].食品工程,2016,(3):35-40+57.
[6]Manna D K,Nandi A K,Manabendra Pattanayak,et al.A water soluble β-glucan of an edible mushroom Termitomyces heimii:Structural and biological investigation[J].Carbohyd Polym,2015,134:375–384.
[7] Zhao H J,Juan L,Zhang J J,et al.Hepatoprotective and in vitro antioxidant effects of native depolymerised-exopolysaccharides derived from Termitomyces albuminosus[J].Sci Rep,2017,7(1):3910-3923.
[8]向枭,陈建,周兴华,等.黄芪多糖对齐口裂腹鱼生长、体组成和免疫指标的影响[J].水生生物学报,2011,35(2):291-299.
[9]夏广清,韩晓娟.黄芪多糖对斑马鱼发育及与衰老相关基因表达的影响[J].中国药学杂志,2012,47(13):1039-1041.
[10]吴旋.四种中草药多糖对黄颡鱼生长、体成分及部分生理生化指标的影响[D].天津:天津农学院,2011.
[11]白东清,吴旋,郭永军,等.长期投喂黄芪多糖对黄颡鱼抗氧化及非特异性免疫指标的影响[J].动物营养学报,2011,23(9):1622-1630.
[12]龚全,许国焕,付天玺,等.云芝多糖对奥尼罗非鱼生长、血清溶菌酶活性和补体活性的影响[J].淡水渔业,2008,38(1):16-19.
[13]庞素风,吴志新,王艳,等.云芝多糖对银鲫非特异性免疫功能的影响[J].淡水渔业,2008,38(2):17-22.
[14]陈昌福,姚娟,李兆文.免疫多糖的理化特性及其对水产动物免疫系统的作用机理[J].淡水渔业,2004,34(1):59-61.
[2]黄玉章.黄芪多糖对奥尼罗非鱼生长性能和免疫功能的影响[D].福州:福建农林大学,2009.
[3]王思芦,汪开毓,耿毅,等.鸡枞菌多糖对小鼠T细胞免疫功能的影响[J].中国兽医科学,2013,43(1):77-83.
[4]王思芦,汪开毓,赵玲,等.鸡枞菌多糖对免疫抑制小鼠免疫功能的影响[J].中国药理学通报,2013,29(1):59-63.
[5]卢彩会,郭晓萌,牟德华.黑皮鸡枞菌水提物和醇提物的主要成分及抗氧化性研究[J].食品工程,2016,(3):35-40+57.
[6]Manna D K,Nandi A K,Manabendra Pattanayak,et al.A water soluble β-glucan of an edible mushroom Termitomyces heimii:Structural and biological investigation[J].Carbohyd Polym,2015,134:375–384.
[7] Zhao H J,Juan L,Zhang J J,et al.Hepatoprotective and in vitro antioxidant effects of native depolymerised-exopolysaccharides derived from Termitomyces albuminosus[J].Sci Rep,2017,7(1):3910-3923.
[8]向枭,陈建,周兴华,等.黄芪多糖对齐口裂腹鱼生长、体组成和免疫指标的影响[J].水生生物学报,2011,35(2):291-299.
[9]夏广清,韩晓娟.黄芪多糖对斑马鱼发育及与衰老相关基因表达的影响[J].中国药学杂志,2012,47(13):1039-1041.
[10]吴旋.四种中草药多糖对黄颡鱼生长、体成分及部分生理生化指标的影响[D].天津:天津农学院,2011.
[11]白东清,吴旋,郭永军,等.长期投喂黄芪多糖对黄颡鱼抗氧化及非特异性免疫指标的影响[J].动物营养学报,2011,23(9):1622-1630.
[12]龚全,许国焕,付天玺,等.云芝多糖对奥尼罗非鱼生长、血清溶菌酶活性和补体活性的影响[J].淡水渔业,2008,38(1):16-19.
[13]庞素风,吴志新,王艳,等.云芝多糖对银鲫非特异性免疫功能的影响[J].淡水渔业,2008,38(2):17-22.
[14]陈昌福,姚娟,李兆文.免疫多糖的理化特性及其对水产动物免疫系统的作用机理[J].淡水渔业,2004,34(1):59-61.
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