大亚湾大鹏澳牡蛎养殖临近海域自养微微型浮游生物种群分布特征
|
摘要
于2013年5月到2014年6月,在大亚湾大鹏澳牡蛎区及邻近海域开展了为期14个月的采样调查,利用高效液相色谱(HPLC)法对表层水体中微微型浮游植物(0.7—2.7μm)光合色素进行测定,并应用色素化学分类软件CHEMTAX对自养微微型浮游生物(aototrophicpicoplankton,APP)功能类群进行分析。结果表明,该海域APP中共检出了15种光合色素,其中青绿藻素(Pras)和玉米黄素(Zea)是微微型色素中浓度最高的2种特征色素,均具有明显的季节变化特征:Pras主要出现在低温季节(牡蛎养殖期),而Zea主要出现在高温季节(非牡蛎养殖期)。CHEMTAX分析表明,大鹏澳海域APP最主要的类群是硅藻、蓝藻和青绿藻,而甲藻、隐藻、定鞭金藻、绿藻和金藻生物量较低。温度和营养盐浓度是影响大鹏澳海域APP的时空分布的重要因素,青绿藻主要出现在低温季节(主要在冬季牡蛎养殖期间),且其生物量与溶解无机氮呈显著正相关;而蓝藻则主要出现在高温季节,与温度呈显著正相关。另外,贝类养殖也是能够影响APP空间分布的重要因素,在大鹏澳海域牡蛎养殖期间,青绿藻生物量在养殖区明显高于非养殖海域。
Aototrophic picoplankton(APP) pigments in sea surface layer in oyster area and adjacent area of Dapeng Cove,near Daya Bay,Guangdong,South China,were analyzed in high-performance liquid chromatography(HPLC) in four stations from May 2013 to June 2014.We identified the contributions of different picophytoplankton assemblages to chlorophyll a using Chemical Taxonomy software(CHEMTAX).Fifteen pico-sized phytoplankton pigments were detected.Prasinoxanthin and zeaxanthin were the two major pigments,and both of them had obvious seasonal variation.Prasinoxanthin occurred mainly in low temperature seasons(oyster culture period),and zeaxanthin in the high temperature seasons(non-culture period).CHEMTAX results shows the community of APP comprised mainly diatoms,cyanobacteria,prasinophytes,and the biomass of dinoflagellates,cryptophytes,haptophytes,chrysophytes and chlorophytes were quite low.The concentration of nutrients and temperature were important factors affecting the spatial and temporal distribution of APP in Dapeng Cove.Prasinophytes occurred mainly in the low temperature seasons(mainly during winter oyster culture period),and its biomass was significantly positively correlated with dissolved inorganic nitrogen;while cyanobacteria occurred mainly in the high temperature seasons,and significantly positively correlated with temperature.In addition,shellfish culture was also an important factor that could affect the spatial distribution of APP.During the oyster culture period,the biomass of prasinophytes was significantly higher in the culture area than in the non-aquaculture area.
Aototrophic picoplankton(APP) pigments in sea surface layer in oyster area and adjacent area of Dapeng Cove,near Daya Bay,Guangdong,South China,were analyzed in high-performance liquid chromatography(HPLC) in four stations from May 2013 to June 2014.We identified the contributions of different picophytoplankton assemblages to chlorophyll a using Chemical Taxonomy software(CHEMTAX).Fifteen pico-sized phytoplankton pigments were detected.Prasinoxanthin and zeaxanthin were the two major pigments,and both of them had obvious seasonal variation.Prasinoxanthin occurred mainly in low temperature seasons(oyster culture period),and zeaxanthin in the high temperature seasons(non-culture period).CHEMTAX results shows the community of APP comprised mainly diatoms,cyanobacteria,prasinophytes,and the biomass of dinoflagellates,cryptophytes,haptophytes,chrysophytes and chlorophytes were quite low.The concentration of nutrients and temperature were important factors affecting the spatial and temporal distribution of APP in Dapeng Cove.Prasinophytes occurred mainly in the low temperature seasons(mainly during winter oyster culture period),and its biomass was significantly positively correlated with dissolved inorganic nitrogen;while cyanobacteria occurred mainly in the high temperature seasons,and significantly positively correlated with temperature.In addition,shellfish culture was also an important factor that could affect the spatial distribution of APP.During the oyster culture period,the biomass of prasinophytes was significantly higher in the culture area than in the non-aquaculture area.
引文
于宗赫,江涛,夏建军等,2014.大鹏澳牡蛎养殖区生态服务价值评估.水产学报,38(6):853-860
王芳,董双林,张硕,2000.海湾扇贝和太平洋牡蛎的食物选择性及滤除率的实验研究.海洋与湖沼,31(2):139-144
张玲,李政菊,陈飞羽等,2015.大鹏澳牡蛎养殖对浮游植物种群结构的影响研究.海洋与湖沼,46(3):549-555
张勇,张永丰,张万磊等,2012.秦皇岛海域微微型藻华期间叶绿素a分级研究.生态科学,31(4):357-363
陈纪新,黄邦钦,贾锡伟等,2003.利用光合色素研究厦门海域超微型浮游植物群落结构.海洋环境科学,22(3):16-21
郑耀洋,2016.渤海中部海域浮游植物种群结构、光合色素和粒径特征研究.广州:暨南大学硕士学位论文
郭皓,闫启仑,沈亮夫,1999.贝类浮筏养殖区内、外浮游植物生态特征研究.黄渤海海洋,17(3):64-72
Barber R T,2007.Oceans:picoplankton do some heavy lifting.Science,315(5813):777-778
Bec B,Collos Y,Souchu P et al,2011.Distribution of picophytoplankton and nanophytoplankton along an anthropogenic eutrophication gradient in French Mediterranean coastal lagoons.Aquatic Microbial Ecology,63(1):29-45
Calvo-Díaz A,Morán X A G,2006.Seasonal dynamics of picoplankton in shelf waters of the southern Bay of Biscay.Aquatic Microbial Ecology,42(2):159-174
Chisholm S W,Olson R J,Zettler E R et al,1988.A novel free-living prochlorophyte abundant in the oceanic euphotic zone.Nature,334(6180):340-343
Cloern J E,1982.Does the benthos control phytoplankton biomass in South San Francisco bay?Marine Ecology Progress Series,9:191-202
Cranford P J,Li W,Strand?et al,2008.Phytoplankton depletion by mussel aquaculture:high resolution mapping,ecosystem modeling,and potential indicators of ecological carrying capacity.ICES CM
Cranford P J,Ward J E,Shumway S E,2011.Bivalve filter feeding:variability and limits of the aquaculture biofilter.In:Shumway S E ed.Shellfish Aquaculture and the Environment.Oxford,UK:Wiley-Blackwell,81-124
Deslous-Paoli J M,Héral M,Goulletquer P et al,1987.Evolution saisonniere de la filtration de bivalves intertidaux dans des conditions naturelles.Océanis,13:575-579
Gon?alves-Araujo R,de Souza M S,Tavano V M et al,2018.Phyto-and protozooplankton assemblages and hydrographic variability during an early winter survey in the Southern Brazilian Continental Shelf.Journal of Marine Systems,184:36-49
Guenther M,Araújo M,Flores-Montes M et al,2015.Eutrophication effects on phytoplankton size-fractioned biomass and production at a tropical estuary.Marine Pollution Bulletin,91(2):537-547
Hoegh-Guldberg O,Bruno J F,2010.The impact of climate change on the world's marine ecosystems.Science,328(5985):1523-1528
Huang B Q,Lin X J,Hong H S,2009.Spatial and temporal variations of Synechococcus and picoeukaryotes in the Taiwan Strait,China.Chinese Journal of Oceanology and Limnology,27(1):22-30
Jiang T,Chen F Y,Yu Z H et al,2016.Size-dependent depletion and community disturbance of phytoplankton under intensive oyster mariculture based on HPLC pigment analysis in Daya Bay,South China Sea.Environmental Pollution,219:804-814
Kirkham A R,Jardillier L E,Holland R et al,2011.Analysis of photosynthetic picoeukaryote community structure along an extended Ellett Line transect in the northern North Atlantic reveals a dominance of novel prymnesiophyte and prasinophyte phylotypes.Deep Sea Research Part I:Oceanographic Research Papers,58(7):733-744
Lehane C,Davenport J,2006.A 15-month study of zooplankton ingestion by farmed mussels(Mytilus edulis)in Bantry Bay,Southwest Ireland.Estuarine,Coastal&Shelf Science,67(4):645-652
Li T,Liu S,Huang L M et al,2011.Diatom to dinoflagellate shift in the summer phytoplankton community in a bay impacted by nuclear power plant thermal effluent.Marine Ecology Progress Series,424:75-85
Lohrenz S E,Carroll C L,Weidemann A D et al,2003.Variations in phytoplankton pigments,size structure and community composition related to wind forcing and water mass properties on the North Carolina inner shelf.Continental Shelf Research,23(14-15):1447-1464
Mackey M D,Mackey D J,Higgins H W et al,1996.CHEMTAX-A program for estimating class abundances from chemical markers:Application to HPLC measurements of phytoplankton.Marine Ecology Progress Series,144:265-283
Masquelier S,Foulon E,Jouenne F et al,2011.Distribution of eukaryotic plankton in the English Channel and the North Sea in summer.Journal of Sea Research,66(2):111-122
Massana R,2011.Eukaryotic picoplankton in surface oceans.Annual Review of Microbiology,65(1):91-110
Massana R,BalaguéV,Guillou L et al,2004.Picoeukaryotic diversity in an oligotrophic coastal site studied by molecular and culturing approaches.FEMS Microbiology Ecology,50(3):231-243
Mendes C R B,Tavano V M,Kerr R et al,2018.Impact of sea ice on the structure of phytoplankton communities in the northern Antarctic Peninsula.Deep Sea Research Part II:Topical Studies in Oceanography,149:111-123
Navarro E,Iglesias J I P,Camacho A P et al,1996.The effect of diets of phytoplankton and suspended bottom material on feeding and absorption of raft mussels(Mytilus galloprovincialis Lmk).Journal of Experimental Marine Biology&Ecology,198(2):175-189
Petersen J K,Nielsen T G,van Duren L et al,2008.Depletion of plankton in a raft culture of Mytilus galloprovincialis in Ría de Vigo,NW Spain.I.Phytoplankton.Aquatic Biology,4(2):113-125
Piquet M A T,Bolhuis H,Davidson A T et al,2008.Diversity and dynamics of Antarctic marine microbial eukaryotes under manipulated environmental UV radiation.FEMSMicrobiology Ecology,66(2):352-366
Rajaneesh K M,Mitbavkar S,Anil A C,2017.Influence of short-term hydrographic variations during the north-east monsoon on picophytoplankton community structure in the eastern Arabian Sea.Continental Shelf Research,146:28-36
Reynolds C S,1984.Phytoplankton periodicity:the interactions of form,function and environmental variability.Freshwater Biology,14(2):111-142
Riisg?rd H U,1988.Efficiency of particle retention and filtration rate in 6 species of Northeast American bivalves.Marine Ecology Progress Series,45:217-223
Riisg?rd H U,2001.On measurement of filtration rate in bivalves-the stony road to reliable data:review and interpretation.Marine Ecology Progress Series,211(1):275-291
Safi K A,Hayden B,2010.Differential grazing on natural planktonic populations by the mussel Perna canaliculus.Aquatic Biology,11(2):113-125
Souchu P,Vaquer A,Collos Y et al,2001.Influence of shellfish farming activities on the biogeochemical composition of the water column in Thau lagoon.Marine Ecology Progress Series,218(4):141-152
Tamm M,Laas P,Freiberg R et al,2018.Parallel assessment of marine autotrophic picoplankton using flow cytometry and chemotaxonomy.Science of the Total Environment,625:185-193
Taniuchi Y,Watanabe T,Kakehi S et al,2017.Seasonal dynamics of the phytoplankton community in Sendai Bay,northern Japan.Journal of Oceanography,73(1):1-9
Wang L,Huang B Q,Liu X et al,2015.The modification and optimizing of the CHEMTAX running in the South China Sea.Acta Oceanologica Sinica,34(2):124-131
Wang L H,Ou L J,Huang K X et al,2018.Determination of the spatial and temporal variability of phytoplankton community structure in Daya Bay via HPLC-CHEMTAX pigment analysis.Journal of Oceanology&Limnology,36(3):750-760
Ward J E,Shumway S E,2004.Separating the grain from the chaff:particle selection in suspension-and deposit-feeding bivalves.Journal of Experimental Marine Biology&Ecology,300(1-2):83-130
Worden A Z,2006.Picoeukaryote diversity in coastal waters of the Pacific Ocean.Aquatic Microbial Ecology,43(2):165-175
Yu Z G,Deng C M,Yao P et al,2007.Prasinoxanthin-constaining Prasinophyceae discovered in Jiaozhou Bay,China.Journal of Integrative Plant Biology,49(4):497-506
Zapata M,Rodríguez F,Garrido J L,2000.Separation of chlorophylls and carotenoids from marine phytoplankton:a new HPLC method using a reversed phase C8 column and pyridine-containing mobile phases.Marine Ecology Progress Series,195:29-45
Zhao Y,Zhao L,Xiao T et al,2013.Temporal variation of picoplankton in the spring bloom of Yellow Sea,China.Deep-Sea Research Part II:Topical Studies in Oceanography,97:72-84
Zhao Y,Zhao L,Zhang W C et al,2016.Variations of picoplankton abundances during blooms in the East China Sea.Deep Sea Research Part II:Topical Studies in Oceanography,124:100-108
王芳,董双林,张硕,2000.海湾扇贝和太平洋牡蛎的食物选择性及滤除率的实验研究.海洋与湖沼,31(2):139-144
张玲,李政菊,陈飞羽等,2015.大鹏澳牡蛎养殖对浮游植物种群结构的影响研究.海洋与湖沼,46(3):549-555
张勇,张永丰,张万磊等,2012.秦皇岛海域微微型藻华期间叶绿素a分级研究.生态科学,31(4):357-363
陈纪新,黄邦钦,贾锡伟等,2003.利用光合色素研究厦门海域超微型浮游植物群落结构.海洋环境科学,22(3):16-21
郑耀洋,2016.渤海中部海域浮游植物种群结构、光合色素和粒径特征研究.广州:暨南大学硕士学位论文
郭皓,闫启仑,沈亮夫,1999.贝类浮筏养殖区内、外浮游植物生态特征研究.黄渤海海洋,17(3):64-72
Barber R T,2007.Oceans:picoplankton do some heavy lifting.Science,315(5813):777-778
Bec B,Collos Y,Souchu P et al,2011.Distribution of picophytoplankton and nanophytoplankton along an anthropogenic eutrophication gradient in French Mediterranean coastal lagoons.Aquatic Microbial Ecology,63(1):29-45
Calvo-Díaz A,Morán X A G,2006.Seasonal dynamics of picoplankton in shelf waters of the southern Bay of Biscay.Aquatic Microbial Ecology,42(2):159-174
Chisholm S W,Olson R J,Zettler E R et al,1988.A novel free-living prochlorophyte abundant in the oceanic euphotic zone.Nature,334(6180):340-343
Cloern J E,1982.Does the benthos control phytoplankton biomass in South San Francisco bay?Marine Ecology Progress Series,9:191-202
Cranford P J,Li W,Strand?et al,2008.Phytoplankton depletion by mussel aquaculture:high resolution mapping,ecosystem modeling,and potential indicators of ecological carrying capacity.ICES CM
Cranford P J,Ward J E,Shumway S E,2011.Bivalve filter feeding:variability and limits of the aquaculture biofilter.In:Shumway S E ed.Shellfish Aquaculture and the Environment.Oxford,UK:Wiley-Blackwell,81-124
Deslous-Paoli J M,Héral M,Goulletquer P et al,1987.Evolution saisonniere de la filtration de bivalves intertidaux dans des conditions naturelles.Océanis,13:575-579
Gon?alves-Araujo R,de Souza M S,Tavano V M et al,2018.Phyto-and protozooplankton assemblages and hydrographic variability during an early winter survey in the Southern Brazilian Continental Shelf.Journal of Marine Systems,184:36-49
Guenther M,Araújo M,Flores-Montes M et al,2015.Eutrophication effects on phytoplankton size-fractioned biomass and production at a tropical estuary.Marine Pollution Bulletin,91(2):537-547
Hoegh-Guldberg O,Bruno J F,2010.The impact of climate change on the world's marine ecosystems.Science,328(5985):1523-1528
Huang B Q,Lin X J,Hong H S,2009.Spatial and temporal variations of Synechococcus and picoeukaryotes in the Taiwan Strait,China.Chinese Journal of Oceanology and Limnology,27(1):22-30
Jiang T,Chen F Y,Yu Z H et al,2016.Size-dependent depletion and community disturbance of phytoplankton under intensive oyster mariculture based on HPLC pigment analysis in Daya Bay,South China Sea.Environmental Pollution,219:804-814
Kirkham A R,Jardillier L E,Holland R et al,2011.Analysis of photosynthetic picoeukaryote community structure along an extended Ellett Line transect in the northern North Atlantic reveals a dominance of novel prymnesiophyte and prasinophyte phylotypes.Deep Sea Research Part I:Oceanographic Research Papers,58(7):733-744
Lehane C,Davenport J,2006.A 15-month study of zooplankton ingestion by farmed mussels(Mytilus edulis)in Bantry Bay,Southwest Ireland.Estuarine,Coastal&Shelf Science,67(4):645-652
Li T,Liu S,Huang L M et al,2011.Diatom to dinoflagellate shift in the summer phytoplankton community in a bay impacted by nuclear power plant thermal effluent.Marine Ecology Progress Series,424:75-85
Lohrenz S E,Carroll C L,Weidemann A D et al,2003.Variations in phytoplankton pigments,size structure and community composition related to wind forcing and water mass properties on the North Carolina inner shelf.Continental Shelf Research,23(14-15):1447-1464
Mackey M D,Mackey D J,Higgins H W et al,1996.CHEMTAX-A program for estimating class abundances from chemical markers:Application to HPLC measurements of phytoplankton.Marine Ecology Progress Series,144:265-283
Masquelier S,Foulon E,Jouenne F et al,2011.Distribution of eukaryotic plankton in the English Channel and the North Sea in summer.Journal of Sea Research,66(2):111-122
Massana R,2011.Eukaryotic picoplankton in surface oceans.Annual Review of Microbiology,65(1):91-110
Massana R,BalaguéV,Guillou L et al,2004.Picoeukaryotic diversity in an oligotrophic coastal site studied by molecular and culturing approaches.FEMS Microbiology Ecology,50(3):231-243
Mendes C R B,Tavano V M,Kerr R et al,2018.Impact of sea ice on the structure of phytoplankton communities in the northern Antarctic Peninsula.Deep Sea Research Part II:Topical Studies in Oceanography,149:111-123
Navarro E,Iglesias J I P,Camacho A P et al,1996.The effect of diets of phytoplankton and suspended bottom material on feeding and absorption of raft mussels(Mytilus galloprovincialis Lmk).Journal of Experimental Marine Biology&Ecology,198(2):175-189
Petersen J K,Nielsen T G,van Duren L et al,2008.Depletion of plankton in a raft culture of Mytilus galloprovincialis in Ría de Vigo,NW Spain.I.Phytoplankton.Aquatic Biology,4(2):113-125
Piquet M A T,Bolhuis H,Davidson A T et al,2008.Diversity and dynamics of Antarctic marine microbial eukaryotes under manipulated environmental UV radiation.FEMSMicrobiology Ecology,66(2):352-366
Rajaneesh K M,Mitbavkar S,Anil A C,2017.Influence of short-term hydrographic variations during the north-east monsoon on picophytoplankton community structure in the eastern Arabian Sea.Continental Shelf Research,146:28-36
Reynolds C S,1984.Phytoplankton periodicity:the interactions of form,function and environmental variability.Freshwater Biology,14(2):111-142
Riisg?rd H U,1988.Efficiency of particle retention and filtration rate in 6 species of Northeast American bivalves.Marine Ecology Progress Series,45:217-223
Riisg?rd H U,2001.On measurement of filtration rate in bivalves-the stony road to reliable data:review and interpretation.Marine Ecology Progress Series,211(1):275-291
Safi K A,Hayden B,2010.Differential grazing on natural planktonic populations by the mussel Perna canaliculus.Aquatic Biology,11(2):113-125
Souchu P,Vaquer A,Collos Y et al,2001.Influence of shellfish farming activities on the biogeochemical composition of the water column in Thau lagoon.Marine Ecology Progress Series,218(4):141-152
Tamm M,Laas P,Freiberg R et al,2018.Parallel assessment of marine autotrophic picoplankton using flow cytometry and chemotaxonomy.Science of the Total Environment,625:185-193
Taniuchi Y,Watanabe T,Kakehi S et al,2017.Seasonal dynamics of the phytoplankton community in Sendai Bay,northern Japan.Journal of Oceanography,73(1):1-9
Wang L,Huang B Q,Liu X et al,2015.The modification and optimizing of the CHEMTAX running in the South China Sea.Acta Oceanologica Sinica,34(2):124-131
Wang L H,Ou L J,Huang K X et al,2018.Determination of the spatial and temporal variability of phytoplankton community structure in Daya Bay via HPLC-CHEMTAX pigment analysis.Journal of Oceanology&Limnology,36(3):750-760
Ward J E,Shumway S E,2004.Separating the grain from the chaff:particle selection in suspension-and deposit-feeding bivalves.Journal of Experimental Marine Biology&Ecology,300(1-2):83-130
Worden A Z,2006.Picoeukaryote diversity in coastal waters of the Pacific Ocean.Aquatic Microbial Ecology,43(2):165-175
Yu Z G,Deng C M,Yao P et al,2007.Prasinoxanthin-constaining Prasinophyceae discovered in Jiaozhou Bay,China.Journal of Integrative Plant Biology,49(4):497-506
Zapata M,Rodríguez F,Garrido J L,2000.Separation of chlorophylls and carotenoids from marine phytoplankton:a new HPLC method using a reversed phase C8 column and pyridine-containing mobile phases.Marine Ecology Progress Series,195:29-45
Zhao Y,Zhao L,Xiao T et al,2013.Temporal variation of picoplankton in the spring bloom of Yellow Sea,China.Deep-Sea Research Part II:Topical Studies in Oceanography,97:72-84
Zhao Y,Zhao L,Zhang W C et al,2016.Variations of picoplankton abundances during blooms in the East China Sea.Deep Sea Research Part II:Topical Studies in Oceanography,124:100-108
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |