曝二氧化碳气体对近具刺链带藻在高氨氮废水中生长的影响
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摘要
养猪废水中丰富的氨氮可为微藻生长提供充足的养分,但是高质量浓度的氨氮会抑制微藻生长。以近具刺链带藻为对象,考查微藻在不同质量浓度的氨氮废水中的生长情况,同时探讨曝二氧化碳气体对微藻在高氨氮废水中生长的影响。结果表明,当初始氨氮的质量浓度为350～900 mg·L~(-1)时,近具刺链带藻的生长受到明显抑制,培养7 d后,各处理微藻的生物量仅增加0.08～0.13 g·L~(-1)。对不同质量浓度的氨氮废水进行曝二氧化碳气体处理后,微藻的生长情况明显改善,各处理的生物量增加了0.32～0.47 g·L~(-1)。未曝气处理的废水pH值呈下降趋势,7 d后下降了约0.50;而曝气条件下废水的pH值呈先增加后下降趋势,第1天到第2天上升了约1.50,从第2天开始至第7天,下降了约0.40。试验条件下氨氮的去除率在3.76%～20.10%。研究结果证实,过高质量浓度的氨氮确实会抑制微藻的生长,但通过曝二氧化碳气体的形式可以改善微藻在高质量浓度氨氮废水中的生长情况。
The piggery wastewater can provide sufficient nutrient for the growth of microalgae. However, high concentration of ammonium nitrogen in the piggery wastewater may inhibit the growth of microalgae. In order to solve this problem, the inhibition of ammonia on Desmodesmus sp. CHX1 growth was investigated in the wastewater with initial ammonium concentration of 350-900 mg·L~(-1), as well as the elimination effects of aeration with carbon dioxide. Results showed that the growth of Desmodesmus sp. CHX1 was inhibited by ammonia, with the increment of biomass of only 0.08-0.13 g·L~(-1) after 7 days of culturing. Aeration with carbon dioxide could alleviate the inhibition of ammonia, with biomass increment of 0.32-0.47 g·L~(-1). Decreasing trends of pH were observed in the wastewater without aeration, with the reduction of 0.5. While the pH in the aeration condition increased by 1.50 first and then decreased by 0.40. The ammonium was removed by 3.76%-20.10% in the experiment conditions. These results demonstrated that high concentration of ammonium nitrogen inhibited the growth of Desmodesmus sp. CHX1. Aeration with carbon dioxide could improve the growth of Desmodesmus sp. CHX1 in the wastewater with high concentration of ammonium nitrogen.
The piggery wastewater can provide sufficient nutrient for the growth of microalgae. However, high concentration of ammonium nitrogen in the piggery wastewater may inhibit the growth of microalgae. In order to solve this problem, the inhibition of ammonia on Desmodesmus sp. CHX1 growth was investigated in the wastewater with initial ammonium concentration of 350-900 mg·L~(-1), as well as the elimination effects of aeration with carbon dioxide. Results showed that the growth of Desmodesmus sp. CHX1 was inhibited by ammonia, with the increment of biomass of only 0.08-0.13 g·L~(-1) after 7 days of culturing. Aeration with carbon dioxide could alleviate the inhibition of ammonia, with biomass increment of 0.32-0.47 g·L~(-1). Decreasing trends of pH were observed in the wastewater without aeration, with the reduction of 0.5. While the pH in the aeration condition increased by 1.50 first and then decreased by 0.40. The ammonium was removed by 3.76%-20.10% in the experiment conditions. These results demonstrated that high concentration of ammonium nitrogen inhibited the growth of Desmodesmus sp. CHX1. Aeration with carbon dioxide could improve the growth of Desmodesmus sp. CHX1 in the wastewater with high concentration of ammonium nitrogen.
引文
[1] WANG B,LI Y Q,WU N,et al.CO2 bio-mitigation using microalgae[J].Applied Microbiology and Biotechnology,2008,79(5):707-718.
[2] PRAGYA N,PANDEY K K,SAHOO P K.A review on harvesting,oil extraction and biofuels production technologies from microalgae[J].Renewable and Sustainable Energy Reviews,2013,24:159-171.
[3] 赵立欣,宋成军,董保成,等.基于微藻养殖的沼液资源化利用与高价值生物质生产耦合技术研究[J].安全与环境学报,2012,12(3):61-65.ZHAO L X,SONG C J,DONG B C,et al.On the microalgae-cultivation based coupling of biogas fermentative liquid-resource utilization and high-quality biomass production[J].Journal of Safety and Environment,2012,12(3):61-65.(in Chinese with English abstract)
[4] 李超,冯玉杰,张大伟,等.以市政污水为底物的微藻油脂积累和碳流分析[J].可再生能源,2012,30(6):93-96.LI C,FENG Y J,ZHANG D W,et al.Microalgae lipid accumulation and carbon flux analysis based on municipal wastewater used as medium[J].Renewable Energy Resources,2012,30(6):93-96.(in Chinese with English abstract)
[5] 程海翔.一株栅藻的分离培养及其应用于养猪废水处理的潜力研究[D].杭州:浙江大学,2013.CHENG H X.Research on isolation and cultivation of a new microalga and the potential of its application for treating piggery wastewater[D].Hangzhou:Zhejiang University,2013.(in Chinese with English abstract)
[6] PRANDINI J M,DA SILVA M L B,MEZZARI M P,et al.Enhancement of nutrient removal from swine wastewater digestate coupled to biogas purification by microalgae Scenedesmus spp[J].Bioresource Technology,2016,202:67-75.
[7] WANG Y,GUO W Q,YEN H W,et al.Cultivation of Chlorella vulgaris JSC-6 with swine wastewater for simultaneous nutrient/COD removal and carbohydrate production[J].Bioresource Technology,2015,198:619-625.
[8] PODEVIN M,DE FRANCISCI D,HOLDT S L,et al.Effect of nitrogen source and acclimatization on specific growth rates of microalgae determined by a high-throughput in vivo microplate autofluorescence method[J].Journal of Applied Phycology,2015,27(4):1415-1423.
[9] PECCIA J,HAZNEDAROGLU B,GUTIERREZ J,et al.Nitrogen supply is an important driver of sustainable microalgae biofuel production[J].Trends in Biotechnology,2013,31(3):134-138.
[10] GUTIERREZ J,KWAN T A,ZIMMERMAN J B,et al.Ammonia inhibition in oleaginous microalgae[J].Algal Research,2016,19:123-127.
[11] 朱伟,张俊,赵联芳.底质中氨氮对沉水植物生长的影响[J].生态环境,2006,15(5):914-920.ZHU W,ZHANG J,ZHAO L F.Effect of ammonia in the sediment on the growth and physiological characteristics of submerged macrophytes[J].Ecology and Environment,2006,15(5):914-920.(in Chinese with English abstract)
[12] 刘炎,石小荣,崔益斌,等.高浓度氨氮胁迫对纤细裸藻的毒性效应[J].环境科学,2013,34(11):4386-4391.LIU Y,SHI X R,CUI Y B,et al.Toxic effects of high concentrations of ammonia on euglena gracilis[J].Environmental Science,2013,34(11):4386-4391.(in Chinese with English abstract)
[13] 张庆东,耿如林,戴晔.规模化猪场清粪工艺比选分析[J].中国畜牧兽医,2013,40(2):232-235.ZHANG Q D,GENG R L,DAI Y.Comparison analysis of dung treatment technology on scale pig farms[J].China Animal Husbandry & Veterinary Medicine,2013,40(2):232-235.(in Chinese with English abstract)
[14] JI M K,KIM H C,SAPIREDDY V R,et al.Simultaneous nutrient removal and lipid production from pretreated piggery wastewater by Chlorella vulgaris YSW-04[J].Applied Microbiology and Biotechnology,2013,97(6):2701-2710.
[15] PARK J,JIN H F,LIM B R,et al.Ammonia removal from anaerobic digestion effluent of livestock waste using green alga Scenedesmus sp[J].Bioresource Technology,2010,101(22):8649-8657.
[16] ULUDAG-DEMIRER S,DEMIRER G N,FREAR C,et al.Anaerobic digestion of dairy manure with enhanced ammonia removal[J].Journal of Environmental Management,2008,86(1):193-200.
[17] ZHOU W G,HU B,LI Y C,et al.Mass cultivation of microalgae on animal wastewater:a sequential two-stage cultivation process for energy crop and omega-3-rich animal feed production[J].Applied Biochemistry and Biotechnology,2012,168(2):348-363.
[18] AZOV Y,GOLDMAN J C.Free ammonia inhibition of algal photosynthesis in intensive cultures[J].Applied and Environmental Microbiology,1982,43(4):735-739.
[19] PEREZ NAVARRO M,AMES W M,NILSSON H,et al.Ammonia binding to the oxygen-evolving complex of photosystem II identifies the solvent-exchangeable oxygen bridge (-oxo) of the manganese tetramer[J].Proceedings of the National Academy of Sciences,2013,110(39):15561-15566.
[20] MARKOU G,DEPRAETERE O,MUYLAERT K.Effect of ammonia on the photosynthetic activity of Arthrospira and Chlorella:a study on chlorophyll fluorescence and electron transport[J].Algal Research,2016,16:449-457.
[21] CHENG H X,TIAN G M.Identification of a newly isolated microalga from a local pond and evaluation of its growth and nutrients removal potential in swine breeding effluent[J].Desalination and Water Treatment,2013,51(13/14/15):2768-2775.
[22] LUO L Z,SHAO Y,LUO S,et al.Nutrient removal from piggery wastewater by Desmodesmus sp.CHX1 and its cultivation conditions optimization[J].Environmental Technology,2018,15:1-8.
[23] YAMAMOTO T,TAKAKI K,KOYAMA T,et al.Long-term stability of partial nitritation of swine wastewater digester liquor and its subsequent treatment by Anammox[J].Bioresource Technology,2008,99(14):6419-6425.
[24] VASCONCELOS FERNANDES T,SHRESTHA R,SUI Y X,et al.Closing domestic nutrient cycles using microalgae[J].Environmental Science & Technology,2015,49(20):12450-12456.
[25] 苗亚楠.高二氧化碳处理在亚硫酸氢钠背景下提高小球藻光合产氢的作用机理研究[D].上海:上海师范大学,2017.MIAO Y N.Study on the action mechanism of improving H2 photoproduction in chlorella pyrenoidosa by high CO2 in the background of NaHSO3 treatment[D].Shanghai:Shanghai Normal University,2017.(in Chinese with English abstract)
[26] BRITTO D T,SIDDIQI M Y,GLASS A D M,et al.Futile transmembrane NH+4 cycling:a cellular hypothesis to explain ammonium toxicity in plants[J].Proceedings of the National Academy of Sciences,2001,98(7):4255-4258.
[27] GOLDMAN J C,DENNETT M R,RILEY C B.Effect of nitrogen-mediated changes in alkalinity on pH control and CO2 supply in intensive microalgal cultures[J].Biotechnology and Bioengineering,1982,24(3):619-631.
[28] MARKOU G,VANDAMME D,MUYLAERT K.Ammonia inhibition on Arthrospira platensis in relation to the initial biomass density and pH[J].Bioresource Technology,2014,166:259-265.
[29] OLGUíN E J,GALICIA S,MERCADO G,et al.Annual productivity of Spirulina(Arthrospira) and nutrient removal in a pig wastewater recycling process under tropical conditions[J].Journal of Applied Phycology,2003,15(2/3):249-257.
[2] PRAGYA N,PANDEY K K,SAHOO P K.A review on harvesting,oil extraction and biofuels production technologies from microalgae[J].Renewable and Sustainable Energy Reviews,2013,24:159-171.
[3] 赵立欣,宋成军,董保成,等.基于微藻养殖的沼液资源化利用与高价值生物质生产耦合技术研究[J].安全与环境学报,2012,12(3):61-65.ZHAO L X,SONG C J,DONG B C,et al.On the microalgae-cultivation based coupling of biogas fermentative liquid-resource utilization and high-quality biomass production[J].Journal of Safety and Environment,2012,12(3):61-65.(in Chinese with English abstract)
[4] 李超,冯玉杰,张大伟,等.以市政污水为底物的微藻油脂积累和碳流分析[J].可再生能源,2012,30(6):93-96.LI C,FENG Y J,ZHANG D W,et al.Microalgae lipid accumulation and carbon flux analysis based on municipal wastewater used as medium[J].Renewable Energy Resources,2012,30(6):93-96.(in Chinese with English abstract)
[5] 程海翔.一株栅藻的分离培养及其应用于养猪废水处理的潜力研究[D].杭州:浙江大学,2013.CHENG H X.Research on isolation and cultivation of a new microalga and the potential of its application for treating piggery wastewater[D].Hangzhou:Zhejiang University,2013.(in Chinese with English abstract)
[6] PRANDINI J M,DA SILVA M L B,MEZZARI M P,et al.Enhancement of nutrient removal from swine wastewater digestate coupled to biogas purification by microalgae Scenedesmus spp[J].Bioresource Technology,2016,202:67-75.
[7] WANG Y,GUO W Q,YEN H W,et al.Cultivation of Chlorella vulgaris JSC-6 with swine wastewater for simultaneous nutrient/COD removal and carbohydrate production[J].Bioresource Technology,2015,198:619-625.
[8] PODEVIN M,DE FRANCISCI D,HOLDT S L,et al.Effect of nitrogen source and acclimatization on specific growth rates of microalgae determined by a high-throughput in vivo microplate autofluorescence method[J].Journal of Applied Phycology,2015,27(4):1415-1423.
[9] PECCIA J,HAZNEDAROGLU B,GUTIERREZ J,et al.Nitrogen supply is an important driver of sustainable microalgae biofuel production[J].Trends in Biotechnology,2013,31(3):134-138.
[10] GUTIERREZ J,KWAN T A,ZIMMERMAN J B,et al.Ammonia inhibition in oleaginous microalgae[J].Algal Research,2016,19:123-127.
[11] 朱伟,张俊,赵联芳.底质中氨氮对沉水植物生长的影响[J].生态环境,2006,15(5):914-920.ZHU W,ZHANG J,ZHAO L F.Effect of ammonia in the sediment on the growth and physiological characteristics of submerged macrophytes[J].Ecology and Environment,2006,15(5):914-920.(in Chinese with English abstract)
[12] 刘炎,石小荣,崔益斌,等.高浓度氨氮胁迫对纤细裸藻的毒性效应[J].环境科学,2013,34(11):4386-4391.LIU Y,SHI X R,CUI Y B,et al.Toxic effects of high concentrations of ammonia on euglena gracilis[J].Environmental Science,2013,34(11):4386-4391.(in Chinese with English abstract)
[13] 张庆东,耿如林,戴晔.规模化猪场清粪工艺比选分析[J].中国畜牧兽医,2013,40(2):232-235.ZHANG Q D,GENG R L,DAI Y.Comparison analysis of dung treatment technology on scale pig farms[J].China Animal Husbandry & Veterinary Medicine,2013,40(2):232-235.(in Chinese with English abstract)
[14] JI M K,KIM H C,SAPIREDDY V R,et al.Simultaneous nutrient removal and lipid production from pretreated piggery wastewater by Chlorella vulgaris YSW-04[J].Applied Microbiology and Biotechnology,2013,97(6):2701-2710.
[15] PARK J,JIN H F,LIM B R,et al.Ammonia removal from anaerobic digestion effluent of livestock waste using green alga Scenedesmus sp[J].Bioresource Technology,2010,101(22):8649-8657.
[16] ULUDAG-DEMIRER S,DEMIRER G N,FREAR C,et al.Anaerobic digestion of dairy manure with enhanced ammonia removal[J].Journal of Environmental Management,2008,86(1):193-200.
[17] ZHOU W G,HU B,LI Y C,et al.Mass cultivation of microalgae on animal wastewater:a sequential two-stage cultivation process for energy crop and omega-3-rich animal feed production[J].Applied Biochemistry and Biotechnology,2012,168(2):348-363.
[18] AZOV Y,GOLDMAN J C.Free ammonia inhibition of algal photosynthesis in intensive cultures[J].Applied and Environmental Microbiology,1982,43(4):735-739.
[19] PEREZ NAVARRO M,AMES W M,NILSSON H,et al.Ammonia binding to the oxygen-evolving complex of photosystem II identifies the solvent-exchangeable oxygen bridge (-oxo) of the manganese tetramer[J].Proceedings of the National Academy of Sciences,2013,110(39):15561-15566.
[20] MARKOU G,DEPRAETERE O,MUYLAERT K.Effect of ammonia on the photosynthetic activity of Arthrospira and Chlorella:a study on chlorophyll fluorescence and electron transport[J].Algal Research,2016,16:449-457.
[21] CHENG H X,TIAN G M.Identification of a newly isolated microalga from a local pond and evaluation of its growth and nutrients removal potential in swine breeding effluent[J].Desalination and Water Treatment,2013,51(13/14/15):2768-2775.
[22] LUO L Z,SHAO Y,LUO S,et al.Nutrient removal from piggery wastewater by Desmodesmus sp.CHX1 and its cultivation conditions optimization[J].Environmental Technology,2018,15:1-8.
[23] YAMAMOTO T,TAKAKI K,KOYAMA T,et al.Long-term stability of partial nitritation of swine wastewater digester liquor and its subsequent treatment by Anammox[J].Bioresource Technology,2008,99(14):6419-6425.
[24] VASCONCELOS FERNANDES T,SHRESTHA R,SUI Y X,et al.Closing domestic nutrient cycles using microalgae[J].Environmental Science & Technology,2015,49(20):12450-12456.
[25] 苗亚楠.高二氧化碳处理在亚硫酸氢钠背景下提高小球藻光合产氢的作用机理研究[D].上海:上海师范大学,2017.MIAO Y N.Study on the action mechanism of improving H2 photoproduction in chlorella pyrenoidosa by high CO2 in the background of NaHSO3 treatment[D].Shanghai:Shanghai Normal University,2017.(in Chinese with English abstract)
[26] BRITTO D T,SIDDIQI M Y,GLASS A D M,et al.Futile transmembrane NH+4 cycling:a cellular hypothesis to explain ammonium toxicity in plants[J].Proceedings of the National Academy of Sciences,2001,98(7):4255-4258.
[27] GOLDMAN J C,DENNETT M R,RILEY C B.Effect of nitrogen-mediated changes in alkalinity on pH control and CO2 supply in intensive microalgal cultures[J].Biotechnology and Bioengineering,1982,24(3):619-631.
[28] MARKOU G,VANDAMME D,MUYLAERT K.Ammonia inhibition on Arthrospira platensis in relation to the initial biomass density and pH[J].Bioresource Technology,2014,166:259-265.
[29] OLGUíN E J,GALICIA S,MERCADO G,et al.Annual productivity of Spirulina(Arthrospira) and nutrient removal in a pig wastewater recycling process under tropical conditions[J].Journal of Applied Phycology,2003,15(2/3):249-257.
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