DPPH法测定树上虾丙酮部位自由基清除能力

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英文篇名：Radical Clearance Ability of Acetone Extract of Pholidota Cantonensis Rolfe Using DPPH Assay 作者：李培源 ; 霍丽妮 ; 彭炳华 ; 莫媛媛 ; 秦一兰 ; 黄晓东 英文作者：Li Peiyuan;Huo Lini;Peng Binghua;Mo Yuanyuan;Qin Yilan;Huang Xiaodong;College of Pharmacy,Guangxi University of Chinese Medicine;College of Chemistry and Materials Science,Guangxi Teachers Education University;The 56th Middle School; 关键词：树上虾 ; DPPH ; 清除能力 ; 总黄酮含量 英文关键词：Pholidota cantonensis rolfe;;DPPH;;clearance ability;;total flavone 中文刊名：山东化工 英文刊名：Shandong Chemical Industry 机构：广西中医药大学药学院;广西师范学院化学与材料学院;广西南宁市第五十六中学; 出版日期：2019-01-08 出版单位：山东化工 年：2019 期：01 基金：广西自然科学基金(2017GXNSFAA198335);; 壮瑶药协同创新中心(桂教科研[2013]20号);; 广西壮瑶药重点实验室(桂科基字[2014]32号);; 广西重点学科壮药学(桂教科研[2013]16号);; 广西八桂学者中药创新理论与药效研究(J13162);; 广西中医药大学校级课题(2017JQ001);广西中医药大学中药学优势学科建设专项课题(ZYX2017003);; 杨世林教授团队人才培养建设项目(YSL17004) 语种：中文; 页：77-78+81 页数：3 CN：37-1212/TQ ISSN：1008-021X 分类号：O657.32;R284

摘要

本论文以丙酮为溶剂,采用冷浸的方式,得到树上虾丙酮提取物。采用DPPH法测定该提取物的自由基清除能力,并测定了该提取物的总黄酮含量。树上虾丙酮提取物的DPPH自由基清除能力与药液浓度和作用时间有关,树上虾丙酮提取物与DPPH自由基的作用迅速,对DPPH清除率最高可达到84. 9%。此外,本论文通过羟自由基法同时研究提取物对其他类型自由基的清除能力,发现该提取物也具有良好的羟自由基清除效果。
        Acetone extract of Pholidota cantonensis Rolfe was obtained using acetone solvent and cold-extraction method. The radical clearance ability of this extract was studied using DPPH assay,and the total flavoniod content was investigated. There are relationships between radical clearance ability and reaction time/concentration. Acetone extract of Pholidota cantonensis Rolfe shows quick reaction with DPPH radicals,and possesses clearance rate as high as 84. 9%. Besides,the clearance ability of the acetone extract of Pholidota cantonensis Rolfe against other radicals was evaluated using hydroxyl radicals,and the extract exhibits good clearance ability.

引文

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