处理含油脂污水对单细胞蛋白合成的影响
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摘要
将全细胞脂肪酶(菌)制剂投加到活性污泥中,用SBR法处理餐厨含油脂污水,研究温度、p H值与活性污泥的SV30值的关系,研究CODCr去除率、油脂去除率与活性污泥中单细胞蛋白含量之间的关系.实验控制温度约为21℃,p H值约为7,活性污泥的SV30值≤40%,活性污泥性能良好; CODCr去除率在24 h时达到最高值99. 33%,油脂去除率在60h时达到最高值99. 37%,油脂去除率和CODCr去除率在急剧增加到一定值后增幅减缓直至稳定.对餐厨含油脂污水和自配含油脂污水的CODCr去除率及油脂去除率进行单因素方差分析,CODCr去除率及油脂去除率均无显著性差异,说明实验中可以用自配含油脂污水代替餐厨含油脂污水.出水中蛋白含量在处理72 h后降到最低值78. 75 87. 50 mg/L,活性污泥中蛋白含量在处理72 h后> 50%,随着餐厨含油脂污水中CODCr和油脂的不断去除,活性污泥中蛋白含量也不断增加,说明在处理含油脂污水同时联产单细胞蛋白是可行的.
The whole cell lipase( bacteria) preparation was added to the activated sludge,the SBR method was used to treat the oily sewage of the kitchen,and the relationship between temperature and p H value and the SV30 value of the activated sludge was studied to study the CODCrremoval rate and the grease removal rate. Relationship with the content of single-cell protein in activated sludge. The experimental control temperature is 21 ℃ approximately,the pH value is 7 approximately,the SV30 value of activated sludge is ≤40%,the performance of activated sludge is good; the removal rate of CODCris 24 h. The highest value was99. 33%,and the grease removal rate reached the highest value of 99. 37% at 60 h. The grease removal rate and CODCrremoval rate increased sharply to a certain value and then slowed down until it stabilized. The CODCrremoval rate and oil removal rate of wastewater were analyzed by one-way ANOVA. There was no significant difference in CODCrremoval rate and oil removal rate,indicating that the oily sewage can be replaced by self-contained oil-containing sewage in the experiment. The protein content in the effluent is after72 h treatment,the minimum value was 78. 75-87. 50 mg/L,and the protein content in the activated sludge was > 50% after 72 h of treatment. With the continuous removal of CODCrand oil in the oily sewage of the kitchen,the eggs in the activated sludge content is increased,the processing described in the fat-containing wastewater cogeneration single cell protein is feasible.
The whole cell lipase( bacteria) preparation was added to the activated sludge,the SBR method was used to treat the oily sewage of the kitchen,and the relationship between temperature and p H value and the SV30 value of the activated sludge was studied to study the CODCrremoval rate and the grease removal rate. Relationship with the content of single-cell protein in activated sludge. The experimental control temperature is 21 ℃ approximately,the pH value is 7 approximately,the SV30 value of activated sludge is ≤40%,the performance of activated sludge is good; the removal rate of CODCris 24 h. The highest value was99. 33%,and the grease removal rate reached the highest value of 99. 37% at 60 h. The grease removal rate and CODCrremoval rate increased sharply to a certain value and then slowed down until it stabilized. The CODCrremoval rate and oil removal rate of wastewater were analyzed by one-way ANOVA. There was no significant difference in CODCrremoval rate and oil removal rate,indicating that the oily sewage can be replaced by self-contained oil-containing sewage in the experiment. The protein content in the effluent is after72 h treatment,the minimum value was 78. 75-87. 50 mg/L,and the protein content in the activated sludge was > 50% after 72 h of treatment. With the continuous removal of CODCrand oil in the oily sewage of the kitchen,the eggs in the activated sludge content is increased,the processing described in the fat-containing wastewater cogeneration single cell protein is feasible.
引文
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[12]宋慧婷,袁珍,王蕊,等.全细胞脂肪酶(菌)对SBR系统中活性污泥性能的影响[J].环境工程学报,2013,7(7):2511-2516.
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[14]刘妮妮. SBR法连续处理油脂加工废水的小试研究[J].中国油脂,2004,29(5):61-66.
[15]张飞,聂麦茜.废弃食用油脂生产单细胞蛋白的研究[J].环保科技,2011,17(3):1-4.
[16]马丹.凯氏定氮法测定食品中蛋白质含量[J].计量与测试技术,2008,35(6):57-58.
[2]李爱阳,蔡玲,朱志杰. PFS絮凝—膜分离法处理含油食品废水的研究[J].环境工程学报,2007,1(10):51-55.
[3]刘国防,梁志伟,杨尚源,等.油脂废水生物处理研究进展[J].应用生态学报,2011,22(8):2219-2226.
[4]刘以珍,葛刚,吴兰.固定化解脂耶氏酵母间歇式处理油脂废水研究[J].环境工程学报,2010,4(3):616-620.
[5]Pendashteh A R,Fakhru’1-Razi A,Chuah T G,et al. Biological treatment of produced water in a sequencing batch reactor by a consortium of isolated halophilic microorganisms[J]. Environ Technol,2010,31(11):1229-1239.
[6]Jeganathan J,Nakhla G,Bassi A. Oily waste water treatment using a novel hybrid PBR-UASB system[J]. Chemosphere,2007,67(8):1492-1501.
[7]Huiting Song,Lichao Zhou,Lujia Zhang,et al. Construction of a whole-cell catalyst displaying a fungal lipase for effective treatment of oily waste water[J]. Journal of Molecular Catalysis B:Enzymatic,2011,71:166-170.
[8] Salvi B L,Panwarn L. Biodiesel resources and production technologies-a review[J]. Renewable and Sustainable Energy Reviews,2012,16(6):368.
[9]覃亚宏,李蕾,唐波,等.油脂含量和有机负荷对餐厨垃圾干式厌氧消化的影响[J].环境工程学报,2016,10(2):887-892.
[10]李寒波.含油废水及其处理技术的研究进展[J].低碳世界,2018,2(2):22-23.
[11]许青青,何成达,余梦宇,等.反硝化法降解油脂废水中的恶臭物质[J].环境工程学报,2017,11(2):867-871.
[12]宋慧婷,袁珍,王蕊,等.全细胞脂肪酶(菌)对SBR系统中活性污泥性能的影响[J].环境工程学报,2013,7(7):2511-2516.
[13]国家环境保护总局.水和废水监测分析方法[M]. 4版.北京:中国环境科学出版社,2002.
[14]刘妮妮. SBR法连续处理油脂加工废水的小试研究[J].中国油脂,2004,29(5):61-66.
[15]张飞,聂麦茜.废弃食用油脂生产单细胞蛋白的研究[J].环保科技,2011,17(3):1-4.
[16]马丹.凯氏定氮法测定食品中蛋白质含量[J].计量与测试技术,2008,35(6):57-58.
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