采煤沉陷裂缝区土壤含水量变化对柠条叶片叶绿素荧光的响应
|
摘要
采煤塌陷引起的土壤环境因子的变化对矿区植物生长的影响越来越受到人们的关注,快速叶绿素荧光诱导动力学分析技术被称为植物受胁迫状态的有效探针,能够快速获取胁迫下光系统II光化学活性和电子传递的信息。研究采煤塌陷裂缝区植物叶片叶绿素荧光的变化是揭示煤炭开采塌陷胁迫对植物个体生长影响的关键环节,能为大尺度下采煤沉陷区植物损伤机理研究提供基础。对于黄土高原半干旱矿区,土壤水分无疑是植物生长最重要的限制因素,而植物叶片叶绿素荧光变化采煤塌陷影响下土壤含水量变化的响应如何尚不清楚。为了弄清采煤沉陷裂缝影响下土壤含水量变化对柠条叶片叶绿素荧光响应的影响,选取神东煤田大柳塔矿区52302工作面为实验场地,在分析了采煤塌陷裂缝对土壤含水量的影响的基础上,以生态修复物种柠条为研究对象,对采煤塌陷裂缝区不同土壤含水量下柠条叶片快速叶绿素荧光诱导动力学曲线进行监测。结果显示:(1)由于煤炭井工开采在地表形成大量裂缝,破坏了土体结构,增加了土壤水分的蒸发面,加速了土壤水的散失。土壤水分含量随着与裂缝之间距离的增加而增加,从距离裂缝0 cm到300 cm,土壤平均含水量从5.63%增加到15.07%;(2)裂缝区土壤水分降低,柠条受到干旱胁迫程度加重,叶片快速叶绿素荧光诱导动力学曲线由O—J—I—P变形为O—K—J—I—P曲线。干旱胁迫通过干扰柠条叶片PSII电子供体侧、受体侧以及电子传递链的功能,严重的损害了柠条叶片光合机构的正常功能。
Increased attention has been given to the influence of changes in environmental factors caused by coal mining subsidence on plant physiology. Rapid chlorophyll fluorescence-induced kinetic analysis is known as an effective probe to examine the stress state of plants, using which the information of photochemical activity and electron transfer of light System II under stress can be quickly obtained. Studying the change of chlorophyll fluorescence in plant leaves from a coal mining fractured area is a key link for revealing the effects of subsidence stress on individual plant growth, and it can provide the basis for studying the damage mechanism in plants from a mining subsidence area. Soil moisture is undoubtedly the most important limiting factor on plant growth, and the response of chlorophyll fluorescence variation in plant leaves to soil water content under the influence of coal mining subsidence is not clear. In order to ascertain the effects of soil water content on the chlorophyll fluorescence response of Caragana korshinskii under the influence of mining subsidence crack, the 52302 working face of the Daliuta mining area was selected as the experimental site with C. korshinskii as the research target. Based on an analysis of the influence of mining subsidence cracks on soil water content, the fast chlorophyll fluorescence induction kinetics curve of C. korshinskii leaves at different soil moisture contents from the coal mining subsidence fractured area was monitored. The effect of soil water content change on the chlorophyll fluorescence response of C. korshinskii leaves under the influence of coal mining subsidence cracks was studied. The results showed that(1) since coal mining destroys the soil structure, a large number of cracks formed on the surface, which increased the evaporation of surface soil moisture, accelerating the loss of soil water. The soil moisture content increased when the distance between two cracks was from 0 cm to 300 cm, and the average water content increased from 5.63% to 15.07%, respectively.(2) The soil moisture in the fractured area further decreased, and C. korshinskii faced drought stress. The rapid chlorophyll fluorescence induced kinetic curve of the leaves was deformed by O—J—I—P to O—K—J—I—P curves. Drought stress seriously interfered with the normal function of the photosynthetic organs in C. korshinskii leaves by interfering with the functions of the PSII electron donor side, receptor side, and the electron transport chain.
Increased attention has been given to the influence of changes in environmental factors caused by coal mining subsidence on plant physiology. Rapid chlorophyll fluorescence-induced kinetic analysis is known as an effective probe to examine the stress state of plants, using which the information of photochemical activity and electron transfer of light System II under stress can be quickly obtained. Studying the change of chlorophyll fluorescence in plant leaves from a coal mining fractured area is a key link for revealing the effects of subsidence stress on individual plant growth, and it can provide the basis for studying the damage mechanism in plants from a mining subsidence area. Soil moisture is undoubtedly the most important limiting factor on plant growth, and the response of chlorophyll fluorescence variation in plant leaves to soil water content under the influence of coal mining subsidence is not clear. In order to ascertain the effects of soil water content on the chlorophyll fluorescence response of Caragana korshinskii under the influence of mining subsidence crack, the 52302 working face of the Daliuta mining area was selected as the experimental site with C. korshinskii as the research target. Based on an analysis of the influence of mining subsidence cracks on soil water content, the fast chlorophyll fluorescence induction kinetics curve of C. korshinskii leaves at different soil moisture contents from the coal mining subsidence fractured area was monitored. The effect of soil water content change on the chlorophyll fluorescence response of C. korshinskii leaves under the influence of coal mining subsidence cracks was studied. The results showed that(1) since coal mining destroys the soil structure, a large number of cracks formed on the surface, which increased the evaporation of surface soil moisture, accelerating the loss of soil water. The soil moisture content increased when the distance between two cracks was from 0 cm to 300 cm, and the average water content increased from 5.63% to 15.07%, respectively.(2) The soil moisture in the fractured area further decreased, and C. korshinskii faced drought stress. The rapid chlorophyll fluorescence induced kinetic curve of the leaves was deformed by O—J—I—P to O—K—J—I—P curves. Drought stress seriously interfered with the normal function of the photosynthetic organs in C. korshinskii leaves by interfering with the functions of the PSII electron donor side, receptor side, and the electron transport chain.
引文
[1] 卞正富.我国煤矿区土地复垦与生态重建研究.资源·产业,2005,7(2):18- 24.
[2] 张发旺,侯新伟,韩占涛,杨会峰,宋亚欣.采煤塌陷对土壤质量的影响效应及保护技术.地理与地理信息科学,2003,19(3):67- 70.
[3] 雷少刚,卞正富.西部干旱区煤炭开采环境影响研究.生态学报,2014,34(11):2837- 2843.
[4] 吴立新,马保东,刘善军.基于SPOT卫星NDVI数据的神东矿区植被覆盖动态变化分析.煤炭学报,2009,34(9):1217- 1222.
[5] 邱汉周.淮南潘集煤矿区植被恢复模式及其土壤修复效应研究[D].长沙:中南林业科技大学,2012:191- 191.
[6] 钱者东,秦卫华,沈明霞,杨昉婧.毛乌素沙地煤矿开采对植被景观的影响.水土保持通报,2014,34(5):299- 303.
[7] Brestic M,Zivcak M,Kalaji H M,Carpentier R,Allakhverdiev S I.Photosystem II thermostability in situ:environmentally induced acclimation and genotype-specific reactions in Triticum aestivum L.Plant Physiology and Biochemistry,2012,57:93- 105.
[8] 苏晓琼,王美月,束胜,孙锦,郭世荣.外源亚精胺对高温胁迫下番茄幼苗快速叶绿素荧光诱导动力学特性的影响.园艺学报,2013,40(12):2409- 2418.
[9] Jedmowski C,Brüggemann W.Imaging of fast chlorophyll fluorescence induction curve (OJIP) parameters,applied in a screening study with wild barley (Hordeum spontaneum) genotypes under heat stress.Journal of Photochemistry and Photobiology B:Biology,2015,151:153- 160.
[10] Mathobo R,Marais D,Steyn J M.The effect of drought stress on yield,leaf gaseous exchange and chlorophyll fluorescence of dry beans (Phaseolus vulgaris L.).Agricultural Water Management,2017,180:118- 125.
[11] Lukatkin A S,Tyutyaev E V,Sharkaeva E S,Lukatkin A A,da Silva J A T.Mild abiotic stresses have different effects on chlorophyll fluorescence parameters in leaves of young woody and herbaceous invasive plants.Acta Physiologiae Plantarum,2017,39(1):20.
[12] Cambridge M L,Zavala-Perez A,Cawthray G R,Mondon J,Kendrick G A.Effects of high salinity from desalination brine on growth,photosynthesis,water relations and osmolyte concentrations of seagrass Posidonia australis.Marine Pollution Bulletin,2017,115(1/2):252- 260.
[13] Marín-Guirao L,Sandoval-Gil J M,Bernardeau-Esteller J,Ruíz J M,Sánchez-Lizaso J L.Responses of the Mediterranean seagrass Posidonia oceanica to hypersaline stress duration and recovery.Marine Environmental Research,2013,84:60- 75.
[14] Marín-Guirao L,Sandoval-Gil J M,Ruíz J M,Sánchez-Lizaso J L.Photosynthesis,growth and survival of the Mediterranean seagrass Posidonia oceanica in response to simulated salinity increases in a laboratory mesocosm system.Estuarine,Coastal and Shelf Science,2011,92(2):286- 296.
[15] Koch M S,Schopmeyer S A,Kyhn-Hansen C,Madden C J,Peters J S.Tropical seagrass species tolerance to hypersalinity stress.Aquatic Botany,2007,86(1):14- 24.
[16] Bian Z F,Lei S G,Inyang H I,Chang L Q,Zhang R C,Zhou C J,He X.Integrated method of RS and GPR for monitoring the changes in the soil moisture and groundwater environment due to underground coal mining.Environmental Geology,2009,57(1):131- 142.
[17] 邹慧.神东风积沙区煤炭开采对土壤水分运移规律的影响[D].北京:中国矿业大学(北京),2014.
[18] 李王成.石羊河流域中游土壤水分运动规律试验研究[D].北京:中国农业大学,2007.
[19] 王力,卫三平,张青峰,王全九,李世清.榆神府矿区土壤-植被-大气系统中水分的稳定性同位素特征.煤炭学报,2010,35(8):1347- 1353.
[20] 王力,卫三平,王全九.榆神府煤田开采对地下水和植被的影响.煤炭学报,2008,33(12):1408- 1414.
[21] 赵宏宇.采煤塌陷对沙质土壤水分特性的影响[D].呼和浩特:内蒙古农业大学,2008.
[22] 郑秀清,樊贵盛.土壤含水率对季节性冻土入渗特性影响的试验研究.农业工程学报,2000,16(6):52- 55.
[23] Bussotti F.Assessment of stress conditions in Quercus ilex L.leaves by O-J-I-P chlorophyll a fluorescence analysis.Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology,2004,138(2):101- 109.
[24] Strasserf R J,Srivastava A.Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria.Photochemistry and Photobiology,1995,61(1):32- 42.
[25] Schansker G,Srivastava A,Govindjee,Strasser R J.Characterization of the 820-nm transmission signal paralleling the chlorophyll a fluorescence rise (OJIP) in pea leaves.Functional Plant Biology,2003,30(7):785- 796.
[26] 王振兴,陈丽,艾军,刘迎雪,何伟,许培磊,秦红艳,赵滢.不同干旱胁迫对山葡萄的光合作用和光系统II活性的影响.植物生理学报,2014,50(8):1171- 1176.
[27] Christen D,Sch?nmann S,Jermini M,Strasser R J,Défago G.Characterization and early detection of grapevine (Vitis vinifera) stress responses to esca disease by in situ chlorophyll fluorescence and comparison with drought stress.Environmental and Experimental Botany,2007,60(3):504- 514.
[28] Oukarroum A,El Madidi S,Schansker G,Strasser R J.Probing the responses of barley cultivars (Hordeum vulgare L.) by chlorophyll a fluorescence OLKJIP under drought stress and re-watering.Environmental and Experimental Botany,2007,60(3):438- 446.
[29] Foyer C H,Noctor G.Oxidant and antioxidant signalling in plants:a re-valuation of the concept of oxidative stress in a physiological context.Plant,Cell & Environment,2005,28(8):1056- 1071.
[30] Johnson G N,Young A J,Scholes J D,Horton P.The dissipation of excess excitation energy in British plant species.Plant,Cell & Environment,1993,16(6):673- 679.
[31] Demmig B,Bj?rkman O.Comparison of the effect of excessive light on chlorophyll fluorescence (77K) and photon yield of O2 evolution in leaves of higher plants.Planta,1987,171(2):171- 184.
[2] 张发旺,侯新伟,韩占涛,杨会峰,宋亚欣.采煤塌陷对土壤质量的影响效应及保护技术.地理与地理信息科学,2003,19(3):67- 70.
[3] 雷少刚,卞正富.西部干旱区煤炭开采环境影响研究.生态学报,2014,34(11):2837- 2843.
[4] 吴立新,马保东,刘善军.基于SPOT卫星NDVI数据的神东矿区植被覆盖动态变化分析.煤炭学报,2009,34(9):1217- 1222.
[5] 邱汉周.淮南潘集煤矿区植被恢复模式及其土壤修复效应研究[D].长沙:中南林业科技大学,2012:191- 191.
[6] 钱者东,秦卫华,沈明霞,杨昉婧.毛乌素沙地煤矿开采对植被景观的影响.水土保持通报,2014,34(5):299- 303.
[7] Brestic M,Zivcak M,Kalaji H M,Carpentier R,Allakhverdiev S I.Photosystem II thermostability in situ:environmentally induced acclimation and genotype-specific reactions in Triticum aestivum L.Plant Physiology and Biochemistry,2012,57:93- 105.
[8] 苏晓琼,王美月,束胜,孙锦,郭世荣.外源亚精胺对高温胁迫下番茄幼苗快速叶绿素荧光诱导动力学特性的影响.园艺学报,2013,40(12):2409- 2418.
[9] Jedmowski C,Brüggemann W.Imaging of fast chlorophyll fluorescence induction curve (OJIP) parameters,applied in a screening study with wild barley (Hordeum spontaneum) genotypes under heat stress.Journal of Photochemistry and Photobiology B:Biology,2015,151:153- 160.
[10] Mathobo R,Marais D,Steyn J M.The effect of drought stress on yield,leaf gaseous exchange and chlorophyll fluorescence of dry beans (Phaseolus vulgaris L.).Agricultural Water Management,2017,180:118- 125.
[11] Lukatkin A S,Tyutyaev E V,Sharkaeva E S,Lukatkin A A,da Silva J A T.Mild abiotic stresses have different effects on chlorophyll fluorescence parameters in leaves of young woody and herbaceous invasive plants.Acta Physiologiae Plantarum,2017,39(1):20.
[12] Cambridge M L,Zavala-Perez A,Cawthray G R,Mondon J,Kendrick G A.Effects of high salinity from desalination brine on growth,photosynthesis,water relations and osmolyte concentrations of seagrass Posidonia australis.Marine Pollution Bulletin,2017,115(1/2):252- 260.
[13] Marín-Guirao L,Sandoval-Gil J M,Bernardeau-Esteller J,Ruíz J M,Sánchez-Lizaso J L.Responses of the Mediterranean seagrass Posidonia oceanica to hypersaline stress duration and recovery.Marine Environmental Research,2013,84:60- 75.
[14] Marín-Guirao L,Sandoval-Gil J M,Ruíz J M,Sánchez-Lizaso J L.Photosynthesis,growth and survival of the Mediterranean seagrass Posidonia oceanica in response to simulated salinity increases in a laboratory mesocosm system.Estuarine,Coastal and Shelf Science,2011,92(2):286- 296.
[15] Koch M S,Schopmeyer S A,Kyhn-Hansen C,Madden C J,Peters J S.Tropical seagrass species tolerance to hypersalinity stress.Aquatic Botany,2007,86(1):14- 24.
[16] Bian Z F,Lei S G,Inyang H I,Chang L Q,Zhang R C,Zhou C J,He X.Integrated method of RS and GPR for monitoring the changes in the soil moisture and groundwater environment due to underground coal mining.Environmental Geology,2009,57(1):131- 142.
[17] 邹慧.神东风积沙区煤炭开采对土壤水分运移规律的影响[D].北京:中国矿业大学(北京),2014.
[18] 李王成.石羊河流域中游土壤水分运动规律试验研究[D].北京:中国农业大学,2007.
[19] 王力,卫三平,张青峰,王全九,李世清.榆神府矿区土壤-植被-大气系统中水分的稳定性同位素特征.煤炭学报,2010,35(8):1347- 1353.
[20] 王力,卫三平,王全九.榆神府煤田开采对地下水和植被的影响.煤炭学报,2008,33(12):1408- 1414.
[21] 赵宏宇.采煤塌陷对沙质土壤水分特性的影响[D].呼和浩特:内蒙古农业大学,2008.
[22] 郑秀清,樊贵盛.土壤含水率对季节性冻土入渗特性影响的试验研究.农业工程学报,2000,16(6):52- 55.
[23] Bussotti F.Assessment of stress conditions in Quercus ilex L.leaves by O-J-I-P chlorophyll a fluorescence analysis.Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology,2004,138(2):101- 109.
[24] Strasserf R J,Srivastava A.Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria.Photochemistry and Photobiology,1995,61(1):32- 42.
[25] Schansker G,Srivastava A,Govindjee,Strasser R J.Characterization of the 820-nm transmission signal paralleling the chlorophyll a fluorescence rise (OJIP) in pea leaves.Functional Plant Biology,2003,30(7):785- 796.
[26] 王振兴,陈丽,艾军,刘迎雪,何伟,许培磊,秦红艳,赵滢.不同干旱胁迫对山葡萄的光合作用和光系统II活性的影响.植物生理学报,2014,50(8):1171- 1176.
[27] Christen D,Sch?nmann S,Jermini M,Strasser R J,Défago G.Characterization and early detection of grapevine (Vitis vinifera) stress responses to esca disease by in situ chlorophyll fluorescence and comparison with drought stress.Environmental and Experimental Botany,2007,60(3):504- 514.
[28] Oukarroum A,El Madidi S,Schansker G,Strasser R J.Probing the responses of barley cultivars (Hordeum vulgare L.) by chlorophyll a fluorescence OLKJIP under drought stress and re-watering.Environmental and Experimental Botany,2007,60(3):438- 446.
[29] Foyer C H,Noctor G.Oxidant and antioxidant signalling in plants:a re-valuation of the concept of oxidative stress in a physiological context.Plant,Cell & Environment,2005,28(8):1056- 1071.
[30] Johnson G N,Young A J,Scholes J D,Horton P.The dissipation of excess excitation energy in British plant species.Plant,Cell & Environment,1993,16(6):673- 679.
[31] Demmig B,Bj?rkman O.Comparison of the effect of excessive light on chlorophyll fluorescence (77K) and photon yield of O2 evolution in leaves of higher plants.Planta,1987,171(2):171- 184.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |