大鼠产前母体冷应激对子代自发、探索及焦虑行为的影响
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摘要
目的:研究产前冷应激对妊娠大鼠子代行为及情绪的影响。方法:将6只SPF级Wister妊娠母鼠,随机分为常温对照组和冷应激组,每组3只。常温对照组妊娠母鼠在(22±2)℃的环境中饲养,冷应激组妊娠母鼠在产前7 d置于人工智能气候室(4±0.1)℃中饲养,待产下幼鼠以后,分为常温对照组公鼠(MR,22只),常温对照组母鼠(FR,15只),冷应激组公鼠(MC,15只),冷应激组母鼠(FC,15只)四组,在子代第四周龄时进行旷场实验、高架十字迷宫实验。结果:在旷场实验中,常温对照组公鼠、母鼠与冷应激组公鼠、母鼠的自发活动、探索行为之间无明显差异(P>0.05)。在高架十字迷宫实验中,冷应激组公鼠、母鼠的开臂滞留时间、开臂进入次数及路程等总体上显著高于常温对照组公鼠、母鼠(P<0.05)。结论:产前母体冷应激对子代自发活动、探索行为及活跃程度无显著影响,但子代出现明显的焦虑行为减少的异常行为。
Objective: To study the effects of prenatal cold stress on the behavior and mood of offspring in pregnant rats. Methods: Six SPF-class Wister pregnant rats were randomly divided into normal temperature control group and cold stress group with 3 rats in each group. The pregnant female rats in the normal temperature control group were kept in the environment of( 22±2) ℃,and the pregnant female rats in the cold stress group were placed in the artificial intelligence climate chamber at( 4±0.1) ℃ for 7 days before the birth,and the young rats were divided into normal temperature after the young rats were born. After the young rats were born,they were divided into normal temperature control group of male rats( MR,22),normal temperature control group of mother rats( FR,15),cold stress group of male rats( MC,15),and cold stress group of female rats( FC,15). In the fourth generation of the offspring,the open field experiment and the elevated cross maze test were carried out. Results: In the open field experiment,there was no significant difference in spontaneous activity and exploration behavior between the normal temperature control group and the cold stress group( P>0.05). In the elevated plus maze experiment,the retention time of the open arms,the number of open arms and the distance of the male and female rats in the cold stress group were significantly higher than those in the normal temperature control group( P<0.05). Conclusion: Prenatal maternal cold stress has no significant effect on spontaneous activity,exploration behavior and activity level of offspring,but the offspring have obvious abnormal behaviors with reduced anxiety behavior.
Objective: To study the effects of prenatal cold stress on the behavior and mood of offspring in pregnant rats. Methods: Six SPF-class Wister pregnant rats were randomly divided into normal temperature control group and cold stress group with 3 rats in each group. The pregnant female rats in the normal temperature control group were kept in the environment of( 22±2) ℃,and the pregnant female rats in the cold stress group were placed in the artificial intelligence climate chamber at( 4±0.1) ℃ for 7 days before the birth,and the young rats were divided into normal temperature after the young rats were born. After the young rats were born,they were divided into normal temperature control group of male rats( MR,22),normal temperature control group of mother rats( FR,15),cold stress group of male rats( MC,15),and cold stress group of female rats( FC,15). In the fourth generation of the offspring,the open field experiment and the elevated cross maze test were carried out. Results: In the open field experiment,there was no significant difference in spontaneous activity and exploration behavior between the normal temperature control group and the cold stress group( P>0.05). In the elevated plus maze experiment,the retention time of the open arms,the number of open arms and the distance of the male and female rats in the cold stress group were significantly higher than those in the normal temperature control group( P<0.05). Conclusion: Prenatal maternal cold stress has no significant effect on spontaneous activity,exploration behavior and activity level of offspring,but the offspring have obvious abnormal behaviors with reduced anxiety behavior.
引文
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[17]王凌星,黄红红,吕国荣.产前850-1900MHz手机暴露对子代大鼠齿状回PCNA和DCX表达的影响[J].中国应用生理学杂志,2018,34(1):35-38.
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[19]Maliszewski-Hall AM,Alexander M,Tká-I,et al.Differential effects of intrauterine growth restriction on the regional neurochemical profile of the developing rat brain[J].Neurochem Res,2017,42(1):133-140.
[20]王凌星,黄红红,陈雅芳,等.产前应激对子代大鼠脑缺血/再灌注后星形胶质细胞的影响[J].中国应用生理学杂志,2016,32(4):301-304.
[21]Gemmel M,Hazlett M,B9gi E,et al.Perinatal fluoxetine effects on social play,the HPA system,and hippocampal plasticity in pre-adolescent male and female rats:interactions with pre-gestational maternal stress[J].Psychoneuroendocrinology,2017,84:159-171.
[22]Lian S,Wang D,Xu B,et al.Prenatal cold stress:Effect on maternal hippocampus and offspring behavior in rats[J].Behav Brain Res,2018,346:1-10.
[2]Umryukhin PE,Grigorchuk OS.Behavior of rats in an open field test as a prognostic indicator of corticosterone levels before and after stress[J].Neuroscience and Behavioral Physiology,2017,47(4):456-458.
[3]Schaffner U,Smith L,Cristofaro M.A review of openfield host range testing to evaluate non-target use by herbivorous biological control candidates[J].Biocontrol,2018,63(3):405-416.
[4]王维刚,吴文婷,周嘉斌,等.小鼠动物实验方法系列专题(五)应用高架十字迷宫分析小鼠焦虑行为[J].中国细胞生物学学报,2011,33(5):466-472.
[5]王维刚,刘震泽,吴文婷,等.小鼠动物实验方法系列专题(七)旷场实验在小鼠行为分析中的应用[J].中国细胞生物学学报,2011,33(11):1191-1196.
[6]Walf AA,Frye CA.Estradiol reduces anxiety-and depression-like behavior of aged female mice[J].Physiol Behav,2010,99(2):169-174.
[7]Masoumi-Ardakani Y,Mahmoudvand H,Mirzaei A,et al.The effect of Elettaria cardamomum extract on anxietylike behavior in a rat model of post-traumatic stress disorder[J].Biomed Pharmacother,2017,87:489-495.
[8]Tazumi T,Hori E,Uwano T,et al.Effects of prenatal maternal stress by repeated cold environment on behavioral and emotional development in the rat offspring[J].Behav Brain Res,2005,162(1):153-160.
[9]Babri S,Doosti MH,Salari AA.Strain-dependent effects of prenatal maternal immune activation on anxiety-and depression-like behaviors in offspring[J].Brain Behav Immun,2014,37:164-176.
[10]Walz K,Spencer C,Kaasik K,et al.Behavioral characterization of mouse models for Smith-Magenis syndrome and dup(17)(p11.2p11.2)[J].Hum Mol Genet,2004,13(4):367-378.
[11]Tazumi T,Hori E,Uwano T,et al.Effects of prenatal maternal stress by repeated cold environment on behavioral and emotional development in the rat offspring[J].Behav Brain Res,2005,162(1):153-160.
[12]Bannerman DM,Deacon RM,Offen S,et al.Double dissociation of function within the hippocampus:spatial memory and hyponeophagia[J].Behav Neurosci,2002,116(5):884-901.
[13]Estanislau C,Morato S.Prenatal stress produces more behavioral alterations than maternal separation in the elevated plus-maze and in the elevated T-maze[J].Behav Brain Res,2005,163(1):70-77.
[14]Lalji H,Almatroudi AMI,Bailey C,et al.Investigating the effects of gender on anxiety related behaviour evoked by kappa opioid receptor activation in the elevated plus maze[J].J Psychopharmacol,2017,31(8):A21.
[15]Van den Hove DL,Leibold NK,Strackx E,et al.Prenatal stress and subsequent exposure to chronic mild stress in rats;interdependent effects on emotional behavior and the serotonergic system[J].Eur Neuropsychopharmcol,2014,24(4):595-607.
[16]Jarvis S,Moinard C,Robson SK,et al.Programming the offspring of the pig by prenatal social stress:neuroendocrine activity and behaviour[J].Horm Behav,2006,49(1):68-80.
[17]王凌星,黄红红,吕国荣.产前850-1900MHz手机暴露对子代大鼠齿状回PCNA和DCX表达的影响[J].中国应用生理学杂志,2018,34(1):35-38.
[18]O’Donnell KJ,Reissland N,Glover V.New Insights into Prenatal Stress:Immediate-and Long-Term Effects on the Fetus and Their Timing[M].Cham:Springer International Publishing,2017:75-85.
[19]Maliszewski-Hall AM,Alexander M,Tká-I,et al.Differential effects of intrauterine growth restriction on the regional neurochemical profile of the developing rat brain[J].Neurochem Res,2017,42(1):133-140.
[20]王凌星,黄红红,陈雅芳,等.产前应激对子代大鼠脑缺血/再灌注后星形胶质细胞的影响[J].中国应用生理学杂志,2016,32(4):301-304.
[21]Gemmel M,Hazlett M,B9gi E,et al.Perinatal fluoxetine effects on social play,the HPA system,and hippocampal plasticity in pre-adolescent male and female rats:interactions with pre-gestational maternal stress[J].Psychoneuroendocrinology,2017,84:159-171.
[22]Lian S,Wang D,Xu B,et al.Prenatal cold stress:Effect on maternal hippocampus and offspring behavior in rats[J].Behav Brain Res,2018,346:1-10.
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