碳化硅量子点荧光标记技术及其生物学应用
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摘要
通过一步法完成SiC量子点的合成和表面改性,并对其微观结构、光学性质和理化性质进行了表征,结果表明该量子点半径小于激子波尔半径,导致了量子限制效应现象而产生光致发光,通过对其红外光谱的分析发现碳化硅量子点表面既已耦合了巯基,因此该量子点可以作为量子点标记技术中又一种新型的标记材料,然后用SiC量子水相溶液对有、无根皮苷环境下的串珠镰刀菌进行标记并长时程荧光成像,同时让已成功标记的该菌侵染苹果植株幼苗,试验表明,根皮苷能够促进串珠镰刀菌的生长,主要表现在菌落成长的速度和数量上,进一步研究发现,串珠镰刀菌生长态势随周围环境中根皮苷含量的增加而更趋旺盛,此外串珠镰刀菌对苹果幼苗侵染的动态示踪过程表明幼苗的第一感染部位为根毛区。
The synthesis and surface modification of SiC quantum dots were completed by one-step method. The microstructure,optical properties and physicochemical properties of SiC quantum dots were characterized. The results show that the size of silicon crabide quantum dots was smaller than its exciton Bohr diameter,which leads to the quantum confinement effect,and the photoluminescence is produced.The infrared spectrum shows that the surface of the silicon carbide quantum dots is coupled with the mercapto group,so it can be used as quantum dot labeling technology is another new type of marking materials,then the fluorescence labeling and long-term fluorescence imaging of fusarium moniliforme grown in the presence or absence of phloridzin were carried out by using the silicon carbide quantum dot aqueous solution,and to complete the mark of the bacteria on the apple plant seedlings for infecting,and to fusarium oxysporum which had marked with SiC QDs infecting apple plant seedings,the results show that the technology of labeling of silicon carbide quantum dots achieved the labeling and long-term tracing to fusarium moniliforme successfully,fusarium moniliforme growth slow and slow split in the environment without phloridzine; but grow quickly,split rapidly under the environment with phlorizin,further studies have found that the growth trend of fusarium moniliforme was related to the environment surrounding,the increase in the content of phlorizin. In addition,the dynamic tracing of fusarium moniliforme to apple seedling indicate that the first infection site of the seedlings was root hair.
The synthesis and surface modification of SiC quantum dots were completed by one-step method. The microstructure,optical properties and physicochemical properties of SiC quantum dots were characterized. The results show that the size of silicon crabide quantum dots was smaller than its exciton Bohr diameter,which leads to the quantum confinement effect,and the photoluminescence is produced.The infrared spectrum shows that the surface of the silicon carbide quantum dots is coupled with the mercapto group,so it can be used as quantum dot labeling technology is another new type of marking materials,then the fluorescence labeling and long-term fluorescence imaging of fusarium moniliforme grown in the presence or absence of phloridzin were carried out by using the silicon carbide quantum dot aqueous solution,and to complete the mark of the bacteria on the apple plant seedlings for infecting,and to fusarium oxysporum which had marked with SiC QDs infecting apple plant seedings,the results show that the technology of labeling of silicon carbide quantum dots achieved the labeling and long-term tracing to fusarium moniliforme successfully,fusarium moniliforme growth slow and slow split in the environment without phloridzine; but grow quickly,split rapidly under the environment with phlorizin,further studies have found that the growth trend of fusarium moniliforme was related to the environment surrounding,the increase in the content of phlorizin. In addition,the dynamic tracing of fusarium moniliforme to apple seedling indicate that the first infection site of the seedlings was root hair.
引文
[1]Botsoa J,Lysenko V,Geloen A.Application of 3C-SiC Quantum Dots for Living Cell Imaging[J].Appled Physics Letters,2008,92:173902(1-3).
[2]李常艳,李倩,刘海涛,等.量子点荧光标记技术的研究热点及面临的挑战[J].生物化学与生物物理进展,2010,37(1):103-110.
[3]沈生文,叶盛英,宋贤良,等.60Co-g辐照改性银掺杂纳米Ti O2及光催化降解乙烯[J].农业工程学报,2012,28(9):236-241.
[4]Chen N,He Y,Su Y,et al.The Cytotoxicity of Cadmium-based Quantum Dots[J].Biomaterials,2012,33(5):1238-1244.
[5]Li Y,Chen C X,Li J T,et al.Surface Charges and Optical Characteristic of Colloidal Cubic Si C Nanocrystals[J].Nanoscale Research Letters,2011(6):454-461.
[6]Fan J Y,Li H X,Jiang J,et al.3C-SiC Nanocrystals as Fluorescent Biological Labels[J].Small,2008,4(8):1058-1062.
[7]Rosales R C P,Pietro D.Vegetative Hyphal Fusion is Not Essential for Plant Infection by Fusarium Oxysporum Eukaryot[J].Cell,2008,(7):162-171.
[8]Manici L M,Ciavatta C,Kelderer M,et al.Replant Problems in South Tyrol:Role of Fungal Pathogens and Microbial Population in Conventional and Organic Apple Orchards[J].Plant and Soil,2003,256:315-324.
[9]孙祥鸣,宋月鹏,高东升,等.碳化硅量子点制备新工艺及其活体细胞荧光成像[J].农业工程学报,2012,28(24):260-264.
[10]康杰,宋月鹏,高东升,等.腐蚀法制备碳化硅量子点标记材料及其光学性能[J].硅酸盐学报,2013,41(12):1714-1719.
[11]宋月鹏,康杰,高东升,等.串珠镰刀菌碳化硅量子点标记及其长时程荧光成像[J].农业工程学报,2013,29(17):286-292.
[12]康杰,宋月鹏,朱彦敏,等.碳化硅量子点表面物化特性调控及其光学特性[J].硅酸盐学报,2015,43(4):431-436.
[13]Sahu T,Ghosh B,Prdhan S K,et al.Diverse Role of Silicon Carbide in the Domain of Nanomaterials[J].International Journal of Electrochemistry,2012.
[14]Mazzola M.Elucidation of The Microbial Complex Having Acausal Role in the Development of Apple Replant Disease in Washington[J].Phytopathology,1998,88:930-938.
[15]Lagopodi A L,Arthur F J R,Lamers G E M,et al.Novel Aspects of Tomato Root Colonization and Infection by Fusarium Oxysporum RadicisLycopersici Revealed by Confocal Laser Scanning Microscopic Analysis Using the Green Fluorescent Protein as a Marker[J].Molecular PlantMicrobe Interactions,2002,15(2):172-179.
[2]李常艳,李倩,刘海涛,等.量子点荧光标记技术的研究热点及面临的挑战[J].生物化学与生物物理进展,2010,37(1):103-110.
[3]沈生文,叶盛英,宋贤良,等.60Co-g辐照改性银掺杂纳米Ti O2及光催化降解乙烯[J].农业工程学报,2012,28(9):236-241.
[4]Chen N,He Y,Su Y,et al.The Cytotoxicity of Cadmium-based Quantum Dots[J].Biomaterials,2012,33(5):1238-1244.
[5]Li Y,Chen C X,Li J T,et al.Surface Charges and Optical Characteristic of Colloidal Cubic Si C Nanocrystals[J].Nanoscale Research Letters,2011(6):454-461.
[6]Fan J Y,Li H X,Jiang J,et al.3C-SiC Nanocrystals as Fluorescent Biological Labels[J].Small,2008,4(8):1058-1062.
[7]Rosales R C P,Pietro D.Vegetative Hyphal Fusion is Not Essential for Plant Infection by Fusarium Oxysporum Eukaryot[J].Cell,2008,(7):162-171.
[8]Manici L M,Ciavatta C,Kelderer M,et al.Replant Problems in South Tyrol:Role of Fungal Pathogens and Microbial Population in Conventional and Organic Apple Orchards[J].Plant and Soil,2003,256:315-324.
[9]孙祥鸣,宋月鹏,高东升,等.碳化硅量子点制备新工艺及其活体细胞荧光成像[J].农业工程学报,2012,28(24):260-264.
[10]康杰,宋月鹏,高东升,等.腐蚀法制备碳化硅量子点标记材料及其光学性能[J].硅酸盐学报,2013,41(12):1714-1719.
[11]宋月鹏,康杰,高东升,等.串珠镰刀菌碳化硅量子点标记及其长时程荧光成像[J].农业工程学报,2013,29(17):286-292.
[12]康杰,宋月鹏,朱彦敏,等.碳化硅量子点表面物化特性调控及其光学特性[J].硅酸盐学报,2015,43(4):431-436.
[13]Sahu T,Ghosh B,Prdhan S K,et al.Diverse Role of Silicon Carbide in the Domain of Nanomaterials[J].International Journal of Electrochemistry,2012.
[14]Mazzola M.Elucidation of The Microbial Complex Having Acausal Role in the Development of Apple Replant Disease in Washington[J].Phytopathology,1998,88:930-938.
[15]Lagopodi A L,Arthur F J R,Lamers G E M,et al.Novel Aspects of Tomato Root Colonization and Infection by Fusarium Oxysporum RadicisLycopersici Revealed by Confocal Laser Scanning Microscopic Analysis Using the Green Fluorescent Protein as a Marker[J].Molecular PlantMicrobe Interactions,2002,15(2):172-179.
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