毒死蜱和重金属对近海沉积物碱性磷酸酶和过氧化氢酶活性的影响
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摘要
选择连云港近海临洪河口河心浅滩沉积物,采用不同浓度毒死蜱和重金属于室内培养,研究了毒死蜱和重金属对沉积物碱性磷酸酶和过氧化氢酶酶活性及其动力学特征的影响。研究结果表明:1)碱性磷酸酶活性受毒死蜱影响显著,峰值出现在10-200mg/kg浓度区间,培养第三周时V_(max)显著下降,酶活性抑制作用最显著。2)毒死蜱对过氧化氢酶激活作用的峰值出现在50mg/kg处。培养三周,过氧化氢酶活性逐渐回归正常值。3)Cd处理对沉积物碱性过氧化氢酶和过氧化氢酶抑制作用显著,明显强于Pb处理以及Cd、Pb复合处理,而Pb在相当程度上缓解了Cd对两种酶的抑制作用。4)酶活性可以用来指示沉积物的早期污染状况。
Effects of pesticide?chlorpyrifos and heavy metals on the activities and kinetics characteristics of alkaline phosphatase and hydrogen peroxidase in sediments from the shoal of Linhong river mouth in Lianyungang were investigated. The results showed that 1) alkaline phosphatase activity was significantly affected by chlorpyrifos with the peak appeared within the concentration range from 10 to 200 mg/kg. After third weeks of culture, V_(max) decreased significantly, and alkaline phosphatase enzyme activity was inhibited significantly; 2) the peak of catalase activaty by chlorpyrifos appeared at 50 mg/kg. After three weeks culture, catalase activity gradually returned to the normal; 3) the inhibition of Cd treatment on alkaline catalase and catalase in sediments was significantly stronger than that of Pb treatment and Cd-Pb complex treatment while Pb alleviated the inhibition of Cd on two enzymes considerably; 4)enzyme activity can be used to indicate early contamination of sediments.
Effects of pesticide?chlorpyrifos and heavy metals on the activities and kinetics characteristics of alkaline phosphatase and hydrogen peroxidase in sediments from the shoal of Linhong river mouth in Lianyungang were investigated. The results showed that 1) alkaline phosphatase activity was significantly affected by chlorpyrifos with the peak appeared within the concentration range from 10 to 200 mg/kg. After third weeks of culture, V_(max) decreased significantly, and alkaline phosphatase enzyme activity was inhibited significantly; 2) the peak of catalase activaty by chlorpyrifos appeared at 50 mg/kg. After three weeks culture, catalase activity gradually returned to the normal; 3) the inhibition of Cd treatment on alkaline catalase and catalase in sediments was significantly stronger than that of Pb treatment and Cd-Pb complex treatment while Pb alleviated the inhibition of Cd on two enzymes considerably; 4)enzyme activity can be used to indicate early contamination of sediments.
引文
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[2] Rao M A,Scelza R,Acevedo F,et al.Enzymes as useful tools for environmental purposes.[J].Chemosphere,Oxford ,2014,107:145-162.
[3] Soldatkin O O,Kucherenko I S,Pyeshkova V M,et al.Novel conductometric biosensor based on three-enzyme system for selective determination of heavy metal ions[J].Bioelectrochemistry,Lausanne,2011,83(1):25-30.
[4] 赵志强,侯宪文,李勤奋,等.毒死蜱和丁硫克百威对香蕉根际土壤酶活性的影响[J].农业环境科学学报,天津,2010,29(b03):98-103.
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[7] Riah W,Laval K,Laroche-Ajzenberg E.Effects of pesticides on soil enzymes:a review[J].Environmental Chemistry Letters,Heidelberg,2014,12(2):257-273.
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[9] 罗虹,刘鹏,宋小敏.重金属镉、铜、镍复合污染对土壤酶活性的影响[J].水土保持学报,杨凌,2006,20(2):94-96.
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[11] 余凯敏,冯为民,李国超,等.毒死蜱的环境生物学效应分析[J].生物技术通报,北京,2015,8:225-230.
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[14] 刘腾飞,邓金花,周峰杰,等.毒死蜱在土壤中的降解及分析研究进展[J].中国农学通报,北京,2014,30(9):26-34.
[15] Wang C,Zhou Z,Liu H,et al.Application of acclimated sewage sludge as a bio-augmentation/bio-stimulation strategy forremediating chlorpyrifos contamination in soil with/without cadmium[J].Science of the Total Environment,Netherlands,2017,579:657-666.
[16] Kumar U,Berliner J,Adak T,et al.Non-target effect of continuous application of chlorpyrifos on soil microbes,nematodes and its persistence under sub-humid tropical rice-rice cropping system.[J].Ecotoxicology & Environmental Safety,SanDiego,2017,135:225-235.
[17] 王川,周巧红,吴振斌.有机磷农药毒死蜱研究进展[J].环境科学与技术,武汉,2011,34(7):123-127.
[18] Mallick K,Bharati K,Banerji A,et al.Bacterial Degradation of Chlorpyrifos in Pure Cultures and in soil [J].Bulletin of Environmental Contamination and Toxicology,United States,1992,62(1):48-54
[19] 倪志鑫,张霞,蔡伟叙,等.珠江口沉积物中重金属分布、形态特征及风险分析[J].海洋环境科学,大连,2016,35(3):321-328.
[20] 安立会,郑丙辉,张雷,等.渤海湾河口沉积物重金属污染及潜在生态风险评价[J].中国环境科学,北京,2010,30(5):666-670.
[21] 刘兴健,葛晨东,崔雁玲,等.连云港潮滩表层沉积物中有机氯农药残留特征与风险评估[J].环境化学,北京,2012,7:966-972.
[22] 李玉,李宏观.连云港近海沉积物重金属历史分布特征及其来源[J].水生态学杂志,武汉,2016,37(6):59-67.
[23] 赵兰坡,姜岩.土壤磷酸酶活性测定方法的探讨[J].土壤通报,沈阳,1986,3:138-141.
[24] 关松荫.土壤酶及其研究法[M].北京:农业出版社.1986
[25] 于庆云,王悠,徐彦,等.镉和铅对菲律宾蛤仔脂质过氧化及抗氧化酶活性的影响[J].生态毒理学报,沈阳,2013,8(4):504-512.
[26] 彭玲,曾江宁,黄伟,等.厚壳贻贝抗氧化酶活性和脂质过氧化物含量对Cd和Aroclor 1254胁迫的响应[J].生态学杂志,沈阳,2015,34(3):718-726.
[27] 孔龙,谭向平,和文祥,等.黄土高原沟壑区宅基地复垦土壤酶动力学研究[J].西北农林科技大学学报(自然科学版),杨凌,2013,41(2):123-129.
[28] 于海霞,戴伟,夏良放,等.亚热带不同人工林对土壤酶活性及其动力学特征的影响[J].北京林业大学学报,北京,2007,29(1):114-118.
[29] 张昀,可欣,张广才,等.乙草胺对土壤脲酶动力学特征的影响[J].植物营养与肥料学报,北京,2012,18(4):915-921.
[30] 张心明,刘贤进,余向阳,等.毒死蜱对土壤中三种酶活性的影响[J].江苏农业学报,南京,2007,23(3):196-199.
[31] 李霞,张小平,喻晓,等.代森锰锌类农药对生姜种植地土壤酶活性及微生物群落结构的影响[J].生态环境学报,2016,25(9):1569-1574.
[31] 李霞,张小平,喻晓,等.代森锰锌类农药对生姜种植地土壤酶活性及微生物群落结构的影响[J].生态环境学报,广州,2016,25(9):1569-1574.
[32] 黄云凤,高扬,毛亮,等.Cd、Pb单一及复合污染下土壤酶生态抑制效应及生态修复基准研究[J].农业环境科学学报,天津,2011,30(11):2258-2264.
[2] Rao M A,Scelza R,Acevedo F,et al.Enzymes as useful tools for environmental purposes.[J].Chemosphere,Oxford ,2014,107:145-162.
[3] Soldatkin O O,Kucherenko I S,Pyeshkova V M,et al.Novel conductometric biosensor based on three-enzyme system for selective determination of heavy metal ions[J].Bioelectrochemistry,Lausanne,2011,83(1):25-30.
[4] 赵志强,侯宪文,李勤奋,等.毒死蜱和丁硫克百威对香蕉根际土壤酶活性的影响[J].农业环境科学学报,天津,2010,29(b03):98-103.
[5] Van Dyk J S,Pletschke B.Review on the use of enzymes for the detection of organochlorine,organophosphate and carbamate pesticides in the environment.[J].Chemosphere,Oxford ,2011,82(3):291-307.
[6] Gianfreda,L.,Rao,M.A..Interactions between xenobiotics and microbial andenzymatic soil activity.critical reviews in environmental science and technology,Tehran,2008,38,269-310.
[7] Riah W,Laval K,Laroche-Ajzenberg E.Effects of pesticides on soil enzymes:a review[J].Environmental Chemistry Letters,Heidelberg,2014,12(2):257-273.
[8] Tejada M,Rodr guez-Morgado B,G mez I,et al.Degradation of chlorpyrifos using different biostimulants/biofertilizers:Effects on soil biochemical properties and microbial community[J].Applied Soil Ecology,Amsterdam,2014,84(84):158-165.
[9] 罗虹,刘鹏,宋小敏.重金属镉、铜、镍复合污染对土壤酶活性的影响[J].水土保持学报,杨凌,2006,20(2):94-96.
[10] 周世萍,段昌群,余泽芬,等.毒死蜱对土壤酶活性的影响[J].土壤通报,沈阳,2008,39(6):1486-1488.
[11] 余凯敏,冯为民,李国超,等.毒死蜱的环境生物学效应分析[J].生物技术通报,北京,2015,8:225-230.
[12] Racke K D,Laskowski D A,Schultz M R.Resistance of chlorpyriphos to enhanced biodegradation in soil[J].Journal of Agricultural and Food Chemistry,Washington,1990,38:1430-1436.
[13] 周世萍,段昌群,韩青辉,等.毒死蜱对土壤蔗糖酶活性的影响[J].生态环境,广州,2005,14(5):672-674.
[14] 刘腾飞,邓金花,周峰杰,等.毒死蜱在土壤中的降解及分析研究进展[J].中国农学通报,北京,2014,30(9):26-34.
[15] Wang C,Zhou Z,Liu H,et al.Application of acclimated sewage sludge as a bio-augmentation/bio-stimulation strategy forremediating chlorpyrifos contamination in soil with/without cadmium[J].Science of the Total Environment,Netherlands,2017,579:657-666.
[16] Kumar U,Berliner J,Adak T,et al.Non-target effect of continuous application of chlorpyrifos on soil microbes,nematodes and its persistence under sub-humid tropical rice-rice cropping system.[J].Ecotoxicology & Environmental Safety,SanDiego,2017,135:225-235.
[17] 王川,周巧红,吴振斌.有机磷农药毒死蜱研究进展[J].环境科学与技术,武汉,2011,34(7):123-127.
[18] Mallick K,Bharati K,Banerji A,et al.Bacterial Degradation of Chlorpyrifos in Pure Cultures and in soil [J].Bulletin of Environmental Contamination and Toxicology,United States,1992,62(1):48-54
[19] 倪志鑫,张霞,蔡伟叙,等.珠江口沉积物中重金属分布、形态特征及风险分析[J].海洋环境科学,大连,2016,35(3):321-328.
[20] 安立会,郑丙辉,张雷,等.渤海湾河口沉积物重金属污染及潜在生态风险评价[J].中国环境科学,北京,2010,30(5):666-670.
[21] 刘兴健,葛晨东,崔雁玲,等.连云港潮滩表层沉积物中有机氯农药残留特征与风险评估[J].环境化学,北京,2012,7:966-972.
[22] 李玉,李宏观.连云港近海沉积物重金属历史分布特征及其来源[J].水生态学杂志,武汉,2016,37(6):59-67.
[23] 赵兰坡,姜岩.土壤磷酸酶活性测定方法的探讨[J].土壤通报,沈阳,1986,3:138-141.
[24] 关松荫.土壤酶及其研究法[M].北京:农业出版社.1986
[25] 于庆云,王悠,徐彦,等.镉和铅对菲律宾蛤仔脂质过氧化及抗氧化酶活性的影响[J].生态毒理学报,沈阳,2013,8(4):504-512.
[26] 彭玲,曾江宁,黄伟,等.厚壳贻贝抗氧化酶活性和脂质过氧化物含量对Cd和Aroclor 1254胁迫的响应[J].生态学杂志,沈阳,2015,34(3):718-726.
[27] 孔龙,谭向平,和文祥,等.黄土高原沟壑区宅基地复垦土壤酶动力学研究[J].西北农林科技大学学报(自然科学版),杨凌,2013,41(2):123-129.
[28] 于海霞,戴伟,夏良放,等.亚热带不同人工林对土壤酶活性及其动力学特征的影响[J].北京林业大学学报,北京,2007,29(1):114-118.
[29] 张昀,可欣,张广才,等.乙草胺对土壤脲酶动力学特征的影响[J].植物营养与肥料学报,北京,2012,18(4):915-921.
[30] 张心明,刘贤进,余向阳,等.毒死蜱对土壤中三种酶活性的影响[J].江苏农业学报,南京,2007,23(3):196-199.
[31] 李霞,张小平,喻晓,等.代森锰锌类农药对生姜种植地土壤酶活性及微生物群落结构的影响[J].生态环境学报,2016,25(9):1569-1574.
[31] 李霞,张小平,喻晓,等.代森锰锌类农药对生姜种植地土壤酶活性及微生物群落结构的影响[J].生态环境学报,广州,2016,25(9):1569-1574.
[32] 黄云凤,高扬,毛亮,等.Cd、Pb单一及复合污染下土壤酶生态抑制效应及生态修复基准研究[J].农业环境科学学报,天津,2011,30(11):2258-2264.
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